FvH4_6g54380

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004578
Physical Location & Seq
Reverse (-)
39717214 .. 39723224
6011 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54380.t1

Sequence Viewer

Length: 975 bp
ATGGCTTCGCAGTCCCAAGGAACATCATCGGAGGGCAGCCCCTCGGCCACGGTGAGCCACGAGTACCGCCTCGCCGTCCAAACCCGTTCCTACACTGAGATTTGGTCAAAGATTCACCAACGAGATGGAGATGAGGAGCTGGTGCTGGAGCAAGTGCTTCAACCCAATCGAGAGTGCGTGGAAGAGGCCCTCCGCCACGCCCAACCCAACTCCCTCACTCGTCTCGTCTCCGACTACTTCCGCCACACCGAGGACGCCACCCACCTCTGTCTGCTCCTCCACCGCAGCGTCTACCGGGCGCGTGACCTCTACGCCCCTCTCCACGAACTCCTCGACGTTTTCGAGGACCTCAGCCTCAACCACTCTCAGTGCACCCACGCTTTTAACGTCTTCACCCAATTCGACCGCCACGACAACCCTTTCCCTTCCCCCGACTCCCACCACAACTTGGACGACACCCGCCGCCTCTTCTCCGACCTCAAGAACAAGCTCGATCGCCGCCTCCGCAAAGCTCGCCGCATTCGCCGGGCCACAGTCATATGTCTGATTGCAACTGCGGTGGGAGTGGTAGGTGCTGCTGCGGCGGCCATCACAGGCCACGCTCTGACGGCTGCGTTTGTTGTGGGCGGGCCTTGTTGTGTGTGCACCAGAAGCAAGGACCATATGAAACAACTGGATGCTGCGGCCAAGGGGACTTGTGTGCTCAACGGTTATCTGGGTAACATTGACAGGCTGGCGCAGCGCCTACGTACGGCGGTGGAGAGTGACAAGCATTTCATTCGGCTGGGATTGGAGAGGGGTGCTGAAGACAAGCATCCCATCCAGGAGGTGCTTAAACAGCTGCGCAAGGGCCACTCCAATTTCCTTCAACTTCTCAATGAACTTGATCAACAAATATGTCTCTGCTTCAACACCGTTAACAGGGCCAGGTCGCTGCTTCTCCAACACATCTATACAACACACTACACTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

325

Amino Acids

36.69

Weight (kDa)

8.32

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 70 - 317 3.2e-10 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 448
AccI GTMKAC 1 cut(s) 291
AccII CGCG 1 cut(s) 301
AcoI YGGCCR 3 cut(s) 45, 585, 684
AcuI CTGAAG 1 cut(s) 825
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 2 cut(s) 65, 751
AfiI CCNNNNNNNGG 4 cut(s) 250, 448, 751, 921
AgsI TTSAA 3 cut(s) 161, 869, 910
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 2 cut(s) 822, 926
AluBI AGCT 4 cut(s) 139, 490, 512, 841
AluI AGCT 4 cut(s) 139, 490, 512, 841
Alw21I GWGCWC 3 cut(s) 374, 647, 705
Alw26I GTCTC 3 cut(s) 227, 232, 905
Alw44I GTGCAC 2 cut(s) 370, 643
AoxI GGCC 9 cut(s) 45, 186, 528, 585, 595, 629, 684, 850, 924
ApaLI GTGCAC 2 cut(s) 370, 643
ApeKI GCWGC 9 cut(s) 36, 285, 575, 578, 611, 680, 739, 841, 934
AspLEI GCGC 4 cut(s) 301, 739, 744, 846
AspS9I GGNCC 7 cut(s) 187, 346, 528, 629, 658, 850, 924
AsuC2I CCSGG 2 cut(s) 296, 527
AsuHPI GGTGA 3 cut(s) 64, 107, 385
AvaII GGWCC 2 cut(s) 346, 658
BaeGI GKGCMC 2 cut(s) 374, 647
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 382, 813
Bbv12I GWGCWC 3 cut(s) 374, 647, 705
BbvCI CCTCAGC 1 cut(s) 350
BbvI GCAGC 9 cut(s) 48, 297, 562, 565, 598, 667, 751, 828, 921
BccI CCATC 3 cut(s) 119, 596, 827
BceAI ACGGC 3 cut(s) 59, 624, 768
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 2 cut(s) 824, 928
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 2 cut(s) 296, 527
BcoDI GTCTC 3 cut(s) 227, 232, 905
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 1 cut(s) 969
BfoI RGCGCY 1 cut(s) 745
Bme1390I CCNGG 4 cut(s) 296, 527, 824, 928
Bme18I GGWCC 2 cut(s) 346, 658
BmgT120I GGNCC 7 cut(s) 187, 346, 528, 629, 658, 850, 924
BmrFI CCNGG 4 cut(s) 296, 527, 824, 928
BmsI GCATC 2 cut(s) 667, 823
BpiI GAAGAC 2 cut(s) 382, 813
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 2 cut(s) 296, 527
BsaAI YACGTR 1 cut(s) 749
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 5 cut(s) 16, 42, 48, 249, 687
Bsc4I CCNNNNNNNGG 4 cut(s) 250, 448, 751, 921
Bse1I ACTGG 1 cut(s) 678
BseBI CCWGG 2 cut(s) 824, 928
BseDI CCNNGG 5 cut(s) 16, 42, 48, 249, 687
BseGI GGATG 3 cut(s) 682, 814, 819
BseLI CCNNNNNNNGG 4 cut(s) 250, 448, 751, 921
BseMII CTCAG 3 cut(s) 87, 364, 380
BseNI ACTGG 1 cut(s) 678
BseRI GAGGAG 3 cut(s) 149, 266, 320
BseSI GKGCMC 2 cut(s) 374, 647
BseXI GCAGC 9 cut(s) 48, 297, 562, 565, 598, 667, 751, 828, 921
BseYI CCCAGC 1 cut(s) 784
Bsh1236I CGCG 1 cut(s) 301
Bsh1285I CGRYCG 2 cut(s) 406, 496
BshFI GGCC 9 cut(s) 47, 188, 530, 587, 597, 631, 686, 852, 926
BsiEI CGRYCG 2 cut(s) 406, 496
BsiHKAI GWGCWC 3 cut(s) 374, 647, 705
BsiSI CCGG 2 cut(s) 295, 526
BsiWI CGTACG 1 cut(s) 749
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 4 cut(s) 250, 448, 751, 921
BsmAI GTCTC 3 cut(s) 227, 232, 905
BsmBI CGTCTC 2 cut(s) 227, 232
BsmFI GGGAC 1 cut(s) 706
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 9 cut(s) 47, 188, 530, 587, 597, 631, 686, 852, 926
Bsp1286I GDGCHC 3 cut(s) 374, 647, 705
Bsp143I GATC 2 cut(s) 493, 886
BspANI GGCC 9 cut(s) 47, 188, 530, 587, 597, 631, 686, 852, 926
BspCNI CTCAG 3 cut(s) 88, 363, 379
BspFNI CGCG 1 cut(s) 301
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 5 cut(s) 16, 42, 48, 249, 687
BssMI GATC 2 cut(s) 493, 886
BssNI GRCGYC 1 cut(s) 255
BssSI CACGAG 1 cut(s) 59
BssT1I CCWWGG 2 cut(s) 16, 687
Bst2BI CACGAG 1 cut(s) 59
Bst2UI CCWGG 2 cut(s) 824, 928
Bst4CI ACNGT 4 cut(s) 52, 535, 710, 916
Bst6I CTCTTC 2 cut(s) 177, 473
BstACI GRCGYC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 514, 629, 735
BstDEI CTNAG 3 cut(s) 96, 350, 366
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 3 cut(s) 682, 814, 819
BstFNI CGCG 1 cut(s) 301
BstH2I RGCGCY 1 cut(s) 745
BstHHI GCGC 4 cut(s) 301, 739, 744, 846
BstKTI GATC 2 cut(s) 496, 889
BstMAI GTCTC 3 cut(s) 227, 232, 905
BstMBI GATC 2 cut(s) 493, 886
BstMCI CGRYCG 2 cut(s) 406, 496
BstMWI GCNNNNNNNGC 9 cut(s) 504, 513, 522, 581, 584, 608, 651, 739, 838
BstNI CCWGG 2 cut(s) 824, 928
BstSCI CCNGG 4 cut(s) 294, 525, 822, 926
BstSLI GKGCMC 2 cut(s) 374, 647
BstSNI TACGTA 1 cut(s) 749
BstUI CGCG 1 cut(s) 301
BstV1I GCAGC 9 cut(s) 48, 297, 562, 565, 598, 667, 751, 828, 921
BstV2I GAAGAC 2 cut(s) 382, 813
BstXI CCANNNNNNTGG 1 cut(s) 125
BsuRI GGCC 9 cut(s) 47, 188, 530, 587, 597, 631, 686, 852, 926
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 3 cut(s) 682, 814, 819
BtsIMutI CAGTG 2 cut(s) 93, 374
Cac8I GCNNGC 3 cut(s) 514, 629, 735
CfoI GCGC 4 cut(s) 301, 739, 744, 846
Cfr13I GGNCC 7 cut(s) 187, 346, 528, 629, 658, 850, 924
CseI GACGC 2 cut(s) 263, 277
Csp6I GTAC 2 cut(s) 64, 750
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviQI GTAC 2 cut(s) 64, 750
DdeI CTNAG 3 cut(s) 96, 350, 366
DpnI GATC 2 cut(s) 495, 888
DpnII GATC 2 cut(s) 493, 886
EaeI YGGCCR 3 cut(s) 45, 585, 684
Eam1104I CTCTTC 2 cut(s) 177, 473
EarI CTCTTC 2 cut(s) 177, 473
EciI GGCGGA 2 cut(s) 182, 230
Eco105I TACGTA 1 cut(s) 749
Eco130I CCWWGG 2 cut(s) 16, 687
Eco47I GGWCC 2 cut(s) 346, 658
Eco57I CTGAAG 1 cut(s) 825
EcoO109I RGGNCCY 2 cut(s) 187, 346
EcoRII CCWGG 2 cut(s) 822, 926
EcoT14I CCWWGG 2 cut(s) 16, 687
ErhI CCWWGG 2 cut(s) 16, 687
Esp3I CGTCTC 2 cut(s) 227, 232
FaiI YATR 6 cut(s) 539, 541, 663, 665, 898, 954
FaqI GGGAC 1 cut(s) 706
FauI CCCGC 2 cut(s) 467, 620
FauNDI CATATG 2 cut(s) 539, 663
FbaI TGATCA 1 cut(s) 886
FblI GTMKAC 1 cut(s) 291
FokI GGATG 3 cut(s) 689, 801, 806
FspBI CTAG 1 cut(s) 969
FspI TGCGCA 1 cut(s) 845
GlaI GCGC 4 cut(s) 300, 738, 743, 845
GsaI CCCAGC 1 cut(s) 788
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 745
HaeIII GGCC 9 cut(s) 47, 188, 530, 587, 597, 631, 686, 852, 926
HapII CCGG 2 cut(s) 295, 526
HgaI GACGC 2 cut(s) 263, 277
HhaI GCGC 4 cut(s) 301, 739, 744, 846
Hin1I GRCGYC 1 cut(s) 255
Hin6I GCGC 4 cut(s) 299, 737, 742, 844
HinP1I GCGC 4 cut(s) 299, 737, 742, 844
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HinfI GANTC 2 cut(s) 112, 434
HpaI GTTAAC 1 cut(s) 919
HpaII CCGG 2 cut(s) 295, 526
HphI GGTGA 3 cut(s) 64, 107, 385
Hpy166II GTNNAC 4 cut(s) 292, 372, 645, 919
Hpy188I TCNGA 5 cut(s) 31, 232, 475, 546, 606
Hpy188III TCNNGA 2 cut(s) 170, 481
Hpy8I GTNNAC 4 cut(s) 292, 372, 645, 919
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 2 cut(s) 435, 875
HpyCH4III ACNGT 4 cut(s) 52, 535, 710, 916
HpyCH4IV ACGT 3 cut(s) 336, 387, 748
HpyCH4V TGCA 3 cut(s) 372, 551, 645
HpyF10VI GCNNNNNNNGC 9 cut(s) 504, 513, 522, 581, 584, 608, 651, 739, 838
HpyF3I CTNAG 3 cut(s) 96, 350, 366
HpySE526I ACGT 3 cut(s) 336, 387, 748
Hsp92I GRCGYC 1 cut(s) 255
HspAI GCGC 4 cut(s) 299, 737, 742, 844
Ksp22I TGATCA 1 cut(s) 886
KspAI GTTAAC 1 cut(s) 919
Kzo9I GATC 2 cut(s) 493, 886
LmnI GCTCC 3 cut(s) 136, 148, 279
Lsp1109I GCAGC 9 cut(s) 48, 297, 562, 565, 598, 667, 751, 828, 921
LweI GCATC 2 cut(s) 667, 823
MaeI CTAG 1 cut(s) 969
MaeII ACGT 3 cut(s) 336, 387, 748
MaeIII GTNAC 3 cut(s) 302, 719, 764
MalI GATC 2 cut(s) 495, 888
MboI GATC 2 cut(s) 493, 886
MboII GAAGA 4 cut(s) 194, 382, 460, 818
MhlI GDGCHC 3 cut(s) 374, 647, 705
MluCI AATT 2 cut(s) 398, 859
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 3 cut(s) 255, 498, 967
MseI TTAA 3 cut(s) 384, 834, 918
MspA1I CMGCKG 1 cut(s) 841
MspI CCGG 2 cut(s) 295, 526
MspR9I CCNGG 4 cut(s) 296, 527, 824, 928
MteI GCGCNGCGC 1 cut(s) 740
Mva1269I GAATGC 1 cut(s) 519
MvaI CCWGG 2 cut(s) 824, 928
MvnI CGCG 1 cut(s) 301
MwoI GCNNNNNNNGC 9 cut(s) 504, 513, 522, 581, 584, 608, 651, 739, 838
NciI CCSGG 2 cut(s) 296, 527
NdeI CATATG 2 cut(s) 539, 663
NdeII GATC 2 cut(s) 493, 886
NmeAIII GCCGAG 1 cut(s) 23
NmuCI GTSAC 2 cut(s) 302, 764
NsbI TGCGCA 1 cut(s) 845
PcsI WCGNNNNNNNCGW 4 cut(s) 330, 339, 384, 408
PctI GAATGC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 112
Pfl23II CGTACG 1 cut(s) 749
PflMI CCANNNNNTGG 1 cut(s) 448
PfoI TCCNGGA 1 cut(s) 822
Ple19I CGATCG 1 cut(s) 496
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 749
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 822, 926
PspFI CCCAGC 1 cut(s) 784
PspGI CCWGG 2 cut(s) 822, 926
PspLI CGTACG 1 cut(s) 749
PspPI GGNCC 7 cut(s) 187, 346, 528, 629, 658, 850, 924
PspPPI RGGWCCY 1 cut(s) 346
PvuI CGATCG 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 841
RsaI GTAC 2 cut(s) 65, 751
RsaNI GTAC 2 cut(s) 64, 750
SaqAI TTAA 3 cut(s) 384, 834, 918
Sau3AI GATC 2 cut(s) 493, 886
Sau96I GGNCC 7 cut(s) 187, 346, 528, 629, 658, 850, 924
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 4 cut(s) 296, 527, 824, 928
SduI GDGCHC 3 cut(s) 374, 647, 705
SfaNI GCATC 2 cut(s) 667, 823
SinI GGWCC 2 cut(s) 346, 658
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SnaBI TACGTA 1 cut(s) 749
SpeI ACTAGT 1 cut(s) 968
Sse9I AATT 2 cut(s) 398, 859
SspMI CTAG 1 cut(s) 969
StyD4I CCNGG 4 cut(s) 294, 525, 822, 926
StyI CCWWGG 2 cut(s) 16, 687
TaaI ACNGT 4 cut(s) 52, 535, 710, 916
TaiI ACGT 3 cut(s) 339, 390, 751
TaqI TCGA 5 cut(s) 169, 333, 342, 402, 492
TasI AATT 2 cut(s) 398, 859
TauI GCSGC 6 cut(s) 465, 501, 519, 584, 587, 686
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 384, 834, 918
Tru9I TTAA 3 cut(s) 384, 834, 918
TscAI CASTG 2 cut(s) 100, 374
TseFI GTSAC 2 cut(s) 302, 764
TseI GCWGC 9 cut(s) 36, 285, 575, 578, 611, 680, 739, 841, 934
Tsp45I GTSAC 2 cut(s) 302, 764
TspDTI ATGAA 3 cut(s) 680, 766, 894
TspRI CASTG 2 cut(s) 100, 374
Van91I CCANNNNNTGG 1 cut(s) 448
VneI GTGCAC 2 cut(s) 370, 643
VpaK11BI GGWCC 2 cut(s) 346, 658
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.