Rorug02G0596300
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004578
Physical Location & Seq
Reverse (-)
71704505 .. 71709507
5003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0596300.1

Sequence Viewer

Length: 2871 bp
ATGGATGAGAAAGCCGAAGCGTTAGAGGCGGTGGTGAAGGAAGCTGTGGATTTGGAGAACGTACCCATTGAAGAGGTGTTTCTGACCTTGAGGTGTGACGAACATGGACTCACATCTGAGGCTGCTCAGCAGAGGCTGACCATTTTTGGGTACAACAAGCTTGAGGAGAAGCAGGAAAGTAAGGTTCTGAAGTTCTTGGGGTTTATGTGGAACCCTCTCTCATGGGTGATGGAAGCTGCAGCTATCATGGCCATTGCTCTTGCCAATGGAGGAGGAAAGCCTCCAGATTGGCAAGACTTTGTTGGGATCATTACTCTGCTCATCATCAATTCAACTATAAGTTTTATTGAAGAGAACAACGCTGGAAATGCTGCTGCTGCTCTCATGGCCCGTCTCGCCCCCAAAGCCAAGGTTCTCCGAGATGGGAGGTGGAATGAGGAGGATGCTTCCGTTCTTGTTCCTGGTGATATAATCAGCATTAAGCTTGGGGACATTATTCCAGCAGATGCTCGCCTCCTCGATGGTGATCCACTGAAAATTGATCAGTCTGCACTTACCGGTGAGTCACTTCCTGTGACCAAAGGCCCTGGTGGCAGTGTGTATGCGGGTTCTACATGCAAACAAGGAGAGATTGAAGCTGTGGTGATTGCCACTGGTGTTCATACCTTTTTCGGCAAGGCTGCTCACCTTGTTGATAGCACGAATCAACAGGGACACTTTCAAAAGGTCTTGACTGCAATTGGGAATTTCTGTATATGTACTATCGCTGTGGGAATGGTCGTAGAGATCATAGTCATGTACCCAGTTCAACACAGAAGTTACCGACCTGGTATTGACAACCTGCTAGTGCTACTTATTGGAGGAATTCCCATTGCAATGCCCACAGTTTTATCTGTGACAATGGCTATTGGTTCCCATCGCTTAGCTGAGCAGGGTGCTATCACTAAACGAATGACGGCAATTGAAGAAATGGCAGGCATGGATGTTCTTTGCAGTGACAAAACTGGGACTCTGACATTAAATAAGCTTTCAGTTGACAAGACTCTTTTAGAGGTCTTTGTTAAAGGGGTGGACATAGATACTGTTGTTCTAATGGCAGCTCGAGCTTCACGAATCGAAAATCAAGATGCTATAGATGCTGCTATTGTTGGAATGTTGGCCGATCCAAAGGAGGCAAGAGCCAATATTCAAGAGGTGCACTTCCTTCCATTCAACCCAACTGACAAGCGAACAGCTCTTACATATATTGACCTTGAAGGTAAAATGCATCGTGTCAGCAAAGGTGCACCGGAACAGATTTTGAATCTTGTACCCAATAAATCAGAGCTAGAACGTAGAGTTCATGTTATGATTGATAAATATGCGGAGCGAGGACTAAGATCCCTAGCAGTAGCATACCAGGAAGTTCCAGATGGAAGGAAGGAGAGCCCAGGAGGGTCTTGGCAATTCATTGCACTCGTGCCTTTGTTTGATCCTCCTAGACATGACAGTGCAGAGACAATTAAAAGAGCATTGAATCTTGGTGTGAATGTTAAGATGATCACAGGTGATCAGCTCGCAATAGCGAAGGAGACAGGACGCCGTCTTGGTATGGGAACAAACATGTACCCCTCATCAGCTCTTTTGGGACAGAAAAAGGATGAGTCACTTGCTGGCTTGCCAGTTGATGAGCTTATTGAAAAAGCTGATGGATTTGCTGGTGTTTTTCCTGAGCACAAGTATGAGATTGTGAAGCGTTTGCAAGCAAGAGGACATATATGTGGGATGACCGGTGATGGAGTTAATGATGCTCCTGCTCTTAAAAAAGCTGATATTGGCATAGCTGTTGCTGATGCAACTGATGCAGCTCGAAGTGCTTCTGACATTGTACTCACAGAACCTGGCCTTAGTGTCATCATAAGCGCTGTACTAACTAGTCGAGCGATATTCCAGAGAATGAAAAATTACACGATATATGCTGTTTCCATTACAATCCGTATTGTGCTTGGTTTCATGTTATTGGCACTCATATGGCACTTCGACTTTCCACCCTTCATGGTTCTGGTCATTGCTATTCTTAATGATGGTACTATCATGACAATTTCGAAGGATAGAGTGAAACCCTCTCCTCTGCCAGACAGTTGGAAACTAGCAGAGATCTTTACCACCGGTATCATCCTTGGTAGTTACCTAGCGGTGATGACGGTCATTTTCTTTTGGGCAGCATACGAAACAGACTTCTTCCCTAGTAAATTTGGGGTTTCAAGCCTTCATAAAAAGGATAACGACAGCTACAGGAAGCTTGCCGCTGCAATGTACCTGCAAGTGAGTACAATCAGTCAGGCTCTCATATTCGTCACACGAGCGCGTAGTTGGTCTTTTATGGAGCGTCCTGGTCTATTACTTGTATCAGCTTTTGTTATTGCTCAGCTGATTGCTACACTCATCGCTGTCTATGCTGATTGGAGTTTCGCTGCGATAGAGGGAATTGGGTGGGGTTGGGCTGGTGTTGTTTGGTTATATAACCTCGTCTTCTATCTGCCACTTGATATTATCAAGTTCTTCATCCGATATGCACTGAGTGGGAGAGCTTGGGATCTCGTATTTGAGCAGAGGACTGCCTTCACAAGGAAGAAGCATTTTGGGAAGGAAGAAAGGGAGCTCAAATGGGCTCACGCGCAGAGGACTCTCCACGGTCTGCAGCCACCGGACACCAAGATGTTCGGTGAACGCGCTTCTAACACAGAACTTAATCAGATGGCCGAAGAGGCAAGAAGACGCGCTGAAATTGCCAGACTGAGGGAGATGCGCACCCTGAAAGGGCATGTCGAATCAGTGGTGAGGCTGAAGGGCCTGGACATCGACACAATTCAGCAATCATACACGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

956

Amino Acids

105.15

Weight (kDa)

6.2

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 20 - 83 1.5e-13 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 132 - 231 1.6e-29 P-type ATPase actuator domain
Hydrolase PF00702 327 - 604 4.8e-15 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 375 - 495 8.2e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2789
Acc36I ACCTGC 2 cut(s) 849, 2307
AccII CGCG 4 cut(s) 2347, 2657, 2712, 2760
AciI CCGC 5 cut(s) 29, 605, 1364, 2174, 2286
AclWI GGATC 6 cut(s) 314, 521, 1157, 1374, 1466, 2583
AcoI YGGCCR 3 cut(s) 249, 1158, 2739
AcsI RAATTY 3 cut(s) 745, 864, 2231
AcuI CTGAAG 2 cut(s) 209, 2846
AcyI GRCGYC 1 cut(s) 1579
AdeI CACNNNGTG 1 cut(s) 2561
AfeI AGCGCT 1 cut(s) 1903
AfiI CCNNNNNNNGG 4 cut(s) 147, 2607, 2778, 2799
AflIII ACRYGT 1 cut(s) 1602
AgeI ACCGGT 3 cut(s) 557, 1769, 2147
AhlI ACTAGT 1 cut(s) 1913
AjnI CCWGG 8 cut(s) 460, 586, 826, 1398, 1429, 1879, 2371, 2832
Alw21I GWGCWC 4 cut(s) 1200, 1288, 1716, 2643
Alw26I GTCTC 3 cut(s) 398, 1490, 1565
Alw44I GTGCAC 2 cut(s) 1196, 1284
AlwI GGATC 6 cut(s) 314, 521, 1157, 1374, 1466, 2583
AlwNI CAGNNNCTG 2 cut(s) 136, 1880
Ama87I CYCGRG 1 cut(s) 1101
Aor51HI AGCGCT 1 cut(s) 1903
AoxI GGCC 7 cut(s) 249, 387, 583, 1158, 1882, 2739, 2830
ApaLI GTGCAC 2 cut(s) 1196, 1284
ApoI RAATTY 3 cut(s) 745, 864, 2231
AsiGI ACCGGT 3 cut(s) 557, 1769, 2147
Asp700I GAANNNNTTC 1 cut(s) 1855
AspLEI GCGC 6 cut(s) 1904, 2347, 2659, 2714, 2762, 2790
AspS9I GGNCC 3 cut(s) 388, 584, 2830
AsuII TTCGAA 1 cut(s) 2084
AvaI CYCGRG 1 cut(s) 1101
BaeGI GKGCMC 2 cut(s) 1200, 1288
BaeI ACNNNNGTAYC 2 cut(s) 1071, 1104
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 3 cut(s) 1430, 2643, 2653
BauI CACGAG 2 cut(s) 1457, 2340
BbsI GAAGAC 2 cut(s) 2503, 2761
Bbv12I GWGCWC 4 cut(s) 1200, 1288, 1716, 2643
BccI CCATC 9 cut(s) 223, 416, 515, 924, 1406, 1682, 1769, 2057, 2731
BceAI ACGGC 2 cut(s) 972, 1566
BciT130I CCWGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
BclI TGATCA 3 cut(s) 541, 1539, 1549
BcoDI GTCTC 3 cut(s) 398, 1490, 1565
BcuI ACTAGT 1 cut(s) 1913
BfaI CTAG 8 cut(s) 845, 1328, 1385, 1479, 1914, 2129, 2171, 2226
BfmI CTRYAG 4 cut(s) 237, 1131, 2272, 2678
BfoI RGCGCY 1 cut(s) 1905
BfuAI ACCTGC 2 cut(s) 849, 2307
BglI GCCNNNNNGGC 2 cut(s) 591, 2747
BglII AGATCT 1 cut(s) 2136
BlpI GCTNAGC 4 cut(s) 126, 922, 927, 2406
Bme1390I CCNGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
BmeT110I CYCGRG 1 cut(s) 1101
BmgT120I GGNCC 3 cut(s) 388, 584, 2830
BmiI GGNNCC 2 cut(s) 212, 913
BmrFI CCNGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
BmrI ACTGGG 2 cut(s) 797, 1014
BmsI GCATC 9 cut(s) 433, 496, 1117, 1126, 1276, 1777, 1822, 1831, 2775
BmuI ACTGGG 2 cut(s) 797, 1014
BpiI GAAGAC 2 cut(s) 2503, 2761
BpmI CTGGAG 1 cut(s) 267
Bpu10I CCTNAGC 1 cut(s) 1710
Bpu1102I GCTNAGC 4 cut(s) 126, 922, 927, 2406
Bpu14I TTCGAA 1 cut(s) 2084
BpuEI CTTGAG 2 cut(s) 109, 182
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 1579
BsaJI CCNNGG 5 cut(s) 408, 586, 1429, 2158, 2671
BsaWI WCCGGW 5 cut(s) 557, 1288, 1769, 2147, 2686
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 2607, 2778, 2799
Bse118I RCCGGY 3 cut(s) 557, 1769, 2147
Bse1I ACTGG 4 cut(s) 658, 803, 1009, 1661
Bse3DI GCAATG 6 cut(s) 252, 870, 882, 1449, 2046, 2298
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
BseDI CCNNGG 5 cut(s) 408, 586, 1429, 2158, 2671
BseGI GGATG 7 cut(s) 10, 448, 988, 1645, 1770, 2154, 2544
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 4 cut(s) 147, 2607, 2778, 2799
BseMI GCAATG 6 cut(s) 252, 870, 882, 1449, 2046, 2298
BseMII CTCAG 7 cut(s) 108, 140, 918, 1701, 2420, 2549, 2768
BseNI ACTGG 4 cut(s) 658, 803, 1009, 1661
BseRI GAGGAG 5 cut(s) 179, 285, 452, 506, 2097
BseSI GKGCMC 2 cut(s) 1200, 1288
BsgI GTGCAG 2 cut(s) 534, 1512
Bsh1236I CGCG 4 cut(s) 2347, 2657, 2712, 2760
BshFI GGCC 7 cut(s) 251, 389, 585, 1160, 1884, 2741, 2832
BshTI ACCGGT 3 cut(s) 557, 1769, 2147
BsiHKAI GWGCWC 4 cut(s) 1200, 1288, 1716, 2643
BsiHKCI CYCGRG 1 cut(s) 1101
BsiSI CCGG 5 cut(s) 558, 1289, 1770, 2148, 2687
BslFI GGGAC 4 cut(s) 503, 726, 1021, 1641
BslI CCNNNNNNNGG 4 cut(s) 147, 2607, 2778, 2799
BsmAI GTCTC 3 cut(s) 398, 1490, 1565
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 4 cut(s) 503, 726, 1021, 1641
BsnI GGCC 7 cut(s) 251, 389, 585, 1160, 1884, 2741, 2832
BsoBI CYCGRG 1 cut(s) 1101
Bsp119I TTCGAA 1 cut(s) 2084
Bsp1286I GDGCHC 6 cut(s) 1200, 1288, 1430, 1716, 2643, 2653
Bsp1720I GCTNAGC 4 cut(s) 126, 922, 927, 2406
BspACI CCGC 5 cut(s) 29, 605, 1364, 2174, 2286
BspANI GGCC 7 cut(s) 251, 389, 585, 1160, 1884, 2741, 2832
BspCNI CTCAG 7 cut(s) 109, 139, 919, 1702, 2419, 2550, 2769
BspFNI CGCG 4 cut(s) 2347, 2657, 2712, 2760
BspHI TCATGA 1 cut(s) 2073
BspLI GGNNCC 2 cut(s) 212, 913
BspMAI CTGCAG 2 cut(s) 241, 2682
BspMI ACCTGC 2 cut(s) 849, 2307
BspPI GGATC 6 cut(s) 314, 521, 1157, 1374, 1466, 2583
BspT104I TTCGAA 1 cut(s) 2084
BsrDI GCAATG 6 cut(s) 252, 870, 882, 1449, 2046, 2298
BsrFI RCCGGY 3 cut(s) 557, 1769, 2147
BsrI ACTGG 4 cut(s) 658, 803, 1009, 1661
BssAI RCCGGY 3 cut(s) 557, 1769, 2147
BssECI CCNNGG 5 cut(s) 408, 586, 1429, 2158, 2671
BssNI GRCGYC 1 cut(s) 1579
BssSI CACGAG 2 cut(s) 1457, 2340
BssT1I CCWWGG 2 cut(s) 408, 2158
Bst2BI CACGAG 2 cut(s) 1457, 2340
Bst2UI CCWGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
Bst4CI ACNGT 7 cut(s) 886, 1084, 1490, 2120, 2185, 2675, 2866
Bst6I CTCTTC 3 cut(s) 66, 345, 2739
BstACI GRCGYC 1 cut(s) 1579
BstAPI GCANNNNNTGC 1 cut(s) 1841
BstBI TTCGAA 1 cut(s) 2084
BstC8I GCNNGC 7 cut(s) 511, 976, 1557, 1654, 1658, 1743, 2283
BstDSI CCRYGG 1 cut(s) 2671
BstENI CCTNNNNNAGG 1 cut(s) 2605
BstF5I GGATG 7 cut(s) 10, 448, 988, 1645, 1770, 2154, 2544
BstFNI CGCG 4 cut(s) 2347, 2657, 2712, 2760
BstH2I RGCGCY 1 cut(s) 1905
BstHHI GCGC 6 cut(s) 1904, 2347, 2659, 2714, 2762, 2790
BstMAI GTCTC 3 cut(s) 398, 1490, 1565
BstNI CCWGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
BstNSI RCATGY 3 cut(s) 618, 1606, 2807
BstSCI CCNGG 8 cut(s) 460, 586, 826, 1398, 1429, 1879, 2371, 2832
BstSFI CTRYAG 4 cut(s) 237, 1131, 2272, 2678
BstSLI GKGCMC 2 cut(s) 1200, 1288
BstUI CGCG 4 cut(s) 2347, 2657, 2712, 2760
BstV2I GAAGAC 2 cut(s) 2503, 2761
BstX2I RGATCY 3 cut(s) 1379, 2136, 2575
BstXI CCANNNNNNTGG 1 cut(s) 2121
BstYI RGATCY 3 cut(s) 1379, 2136, 2575
BsuRI GGCC 7 cut(s) 251, 389, 585, 1160, 1884, 2741, 2832
BtgI CCRYGG 1 cut(s) 2671
BtgZI GCGATG 2 cut(s) 902, 2410
BtsCI GGATG 7 cut(s) 10, 448, 988, 1645, 1770, 2154, 2544
BtsI GCAGTG 2 cut(s) 601, 1000
BtsIMutI CAGTG 7 cut(s) 530, 601, 651, 1000, 1495, 2555, 2820
BveI ACCTGC 2 cut(s) 849, 2307
Cac8I GCNNGC 7 cut(s) 511, 976, 1557, 1654, 1658, 1743, 2283
CaiI CAGNNNCTG 2 cut(s) 136, 1880
CciI TCATGA 1 cut(s) 2073
CfoI GCGC 6 cut(s) 1904, 2347, 2659, 2714, 2762, 2790
Cfr10I RCCGGY 3 cut(s) 557, 1769, 2147
Cfr13I GGNCC 3 cut(s) 388, 584, 2830
CseI GACGC 3 cut(s) 1587, 2357, 2766
CsiI ACCWGGT 1 cut(s) 826
CspAI ACCGGT 3 cut(s) 557, 1769, 2147
DraIII CACNNNGTG 1 cut(s) 2561
EaeI YGGCCR 3 cut(s) 249, 1158, 2739
Eam1104I CTCTTC 3 cut(s) 66, 345, 2739
EarI CTCTTC 3 cut(s) 66, 345, 2739
Ecl136II GAGCTC 1 cut(s) 2641
Eco130I CCWWGG 2 cut(s) 408, 2158
Eco24I GRGCYC 3 cut(s) 1430, 2643, 2653
Eco47III AGCGCT 1 cut(s) 1903
Eco53kI GAGCTC 1 cut(s) 2641
Eco57I CTGAAG 2 cut(s) 209, 2846
Eco88I CYCGRG 1 cut(s) 1101
EcoICRI GAGCTC 1 cut(s) 2641
EcoNI CCTNNNNNAGG 1 cut(s) 2605
EcoO109I RGGNCCY 2 cut(s) 584, 2830
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 8 cut(s) 460, 586, 826, 1398, 1429, 1879, 2371, 2832
EcoT14I CCWWGG 2 cut(s) 408, 2158
EcoT22I ATGCAT 1 cut(s) 1269
EcoT38I GRGCYC 3 cut(s) 1430, 2643, 2653
ErhI CCWWGG 2 cut(s) 408, 2158
Esp3I CGTCTC 1 cut(s) 398
FaqI GGGAC 4 cut(s) 503, 726, 1021, 1641
FauI CCCGC 1 cut(s) 598
FauNDI CATATG 1 cut(s) 2009
FbaI TGATCA 3 cut(s) 541, 1539, 1549
FokI GGATG 7 cut(s) 17, 455, 995, 1652, 1777, 2141, 2531
FriOI GRGCYC 3 cut(s) 1430, 2643, 2653
FspAI RTGCGCAY 1 cut(s) 2789
FspBI CTAG 8 cut(s) 845, 1328, 1385, 1479, 1914, 2129, 2171, 2226
FspI TGCGCA 1 cut(s) 2789
GlaI GCGC 6 cut(s) 1903, 2346, 2658, 2713, 2761, 2789
GsuI CTGGAG 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 1905
HaeIII GGCC 7 cut(s) 251, 389, 585, 1160, 1884, 2741, 2832
HapII CCGG 5 cut(s) 558, 1289, 1770, 2148, 2687
HgaI GACGC 3 cut(s) 1587, 2357, 2766
HhaI GCGC 6 cut(s) 1904, 2347, 2659, 2714, 2762, 2790
Hin1I GRCGYC 1 cut(s) 1579
Hin6I GCGC 6 cut(s) 1902, 2345, 2657, 2712, 2760, 2788
HinP1I GCGC 6 cut(s) 1902, 2345, 2657, 2712, 2760, 2788
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HindIII AAGCTT 4 cut(s) 158, 482, 1025, 2279
HpaII CCGG 5 cut(s) 558, 1289, 1770, 2148, 2687
Hpy166II GTNNAC 5 cut(s) 1036, 1072, 1198, 1286, 2708
Hpy188I TCNGA 9 cut(s) 84, 118, 189, 419, 1014, 1324, 1861, 2549, 2736
Hpy188III TCNNGA 9 cut(s) 284, 730, 1110, 1124, 1190, 1409, 1709, 1930, 2074
Hpy8I GTNNAC 5 cut(s) 1036, 1072, 1198, 1286, 2708
HpyCH4III ACNGT 7 cut(s) 886, 1084, 1490, 2120, 2185, 2675, 2866
HpyCH4IV ACGT 2 cut(s) 60, 1333
HpySE526I ACGT 2 cut(s) 60, 1333
Hsp92I GRCGYC 1 cut(s) 1579
HspAI GCGC 6 cut(s) 1902, 2345, 2657, 2712, 2760, 2788
Ksp22I TGATCA 3 cut(s) 541, 1539, 1549
LmnI GCTCC 4 cut(s) 1366, 1795, 2365, 2638
LweI GCATC 9 cut(s) 433, 496, 1117, 1126, 1276, 1777, 1822, 1831, 2775
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 8 cut(s) 845, 1328, 1385, 1479, 1914, 2129, 2171, 2226
MaeII ACGT 2 cut(s) 60, 1333
MaeIII GTNAC 9 cut(s) 95, 564, 574, 818, 895, 995, 1644, 2165, 2335
MfeI CAATTG 2 cut(s) 738, 960
MflI RGATCY 3 cut(s) 1379, 2136, 2575
MhlI GDGCHC 6 cut(s) 1200, 1288, 1430, 1716, 2643, 2653
MlsI TGGCCA 1 cut(s) 251
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 6 cut(s) 102, 572, 1003, 1036, 1652, 2659
MmeI TCCRAC 2 cut(s) 1129, 2102
Mox20I TGGCCA 1 cut(s) 251
Mph1103I ATGCAT 1 cut(s) 1269
MroXI GAANNNNTTC 1 cut(s) 1855
MscI TGGCCA 1 cut(s) 251
MseI TTAA 9 cut(s) 480, 1019, 1062, 1503, 1533, 1782, 1800, 2058, 2730
MslI CAYNNNNRTG 7 cut(s) 794, 875, 1488, 1719, 1758, 2008, 2696
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 2 cut(s) 2288, 2410
MspI CCGG 5 cut(s) 558, 1289, 1770, 2148, 2687
MspR9I CCNGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
MunI CAATTG 2 cut(s) 738, 960
MvaI CCWGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
MvnI CGCG 4 cut(s) 2347, 2657, 2712, 2760
NdeI CATATG 1 cut(s) 2009
NlaIV GGNNCC 2 cut(s) 212, 913
NmuCI GTSAC 7 cut(s) 95, 564, 574, 895, 995, 1644, 2335
NsbI TGCGCA 1 cut(s) 2789
NsiI ATGCAT 1 cut(s) 1269
NspI RCATGY 3 cut(s) 618, 1606, 2807
NspV TTCGAA 1 cut(s) 2084
PaeR7I CTCGAG 1 cut(s) 1101
PagI TCATGA 1 cut(s) 2073
PciI ACATGT 1 cut(s) 1602
PcsI WCGNNNNNNNCGW 1 cut(s) 1108
PdmI GAANNNNTTC 1 cut(s) 1855
PfeI GAWTC 5 cut(s) 703, 1113, 1303, 1516, 2810
PflFI GACNNNGTC 1 cut(s) 1581
PinAI ACCGGT 3 cut(s) 557, 1769, 2147
PleI GAGTC 6 cut(s) 102, 571, 1003, 1036, 1651, 2659
PpsI GAGTC 6 cut(s) 102, 571, 1003, 1036, 1651, 2659
PscI ACATGT 1 cut(s) 1602
Psp124BI GAGCTC 1 cut(s) 2643
Psp6I CCWGG 8 cut(s) 460, 586, 826, 1398, 1429, 1879, 2371, 2832
PspGI CCWGG 8 cut(s) 460, 586, 826, 1398, 1429, 1879, 2371, 2832
PspN4I GGNNCC 2 cut(s) 212, 913
PspPI GGNCC 3 cut(s) 388, 584, 2830
PspXI VCTCGAGB 1 cut(s) 1101
PsrI GAACNNNNNNTAC 2 cut(s) 1222, 1254
PstI CTGCAG 2 cut(s) 241, 2682
PstNI CAGNNNCTG 2 cut(s) 136, 1880
PsuI RGATCY 3 cut(s) 1379, 2136, 2575
PsyI GACNNNGTC 1 cut(s) 1581
PvuII CAGCTG 1 cut(s) 2410
RseI CAYNNNNRTG 7 cut(s) 794, 875, 1488, 1719, 1758, 2008, 2696
SacI GAGCTC 1 cut(s) 2643
SaqAI TTAA 9 cut(s) 480, 1019, 1062, 1503, 1533, 1782, 1800, 2058, 2730
Sau96I GGNCC 3 cut(s) 388, 584, 2830
SchI GAGTC 6 cut(s) 102, 572, 1003, 1036, 1652, 2659
ScrFI CCNGG 8 cut(s) 462, 588, 828, 1400, 1431, 1881, 2373, 2834
SduI GDGCHC 6 cut(s) 1200, 1288, 1430, 1716, 2643, 2653
SexAI ACCWGGT 1 cut(s) 826
SfaNI GCATC 9 cut(s) 433, 496, 1117, 1126, 1276, 1777, 1822, 1831, 2775
SfcI CTRYAG 4 cut(s) 237, 1131, 2272, 2678
Sfr274I CTCGAG 1 cut(s) 1101
SfuI TTCGAA 1 cut(s) 2084
SlaI CTCGAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 7 cut(s) 794, 875, 1488, 1719, 1758, 2008, 2696
SmlI CTYRAG 3 cut(s) 88, 161, 1101
SmoI CTYRAG 3 cut(s) 88, 161, 1101
SpeI ACTAGT 1 cut(s) 1913
SsiI CCGC 5 cut(s) 29, 605, 1364, 2174, 2286
SspI AATATT 1 cut(s) 1186
SspMI CTAG 8 cut(s) 845, 1328, 1385, 1479, 1914, 2129, 2171, 2226
SstI GAGCTC 1 cut(s) 2643
StyD4I CCNGG 8 cut(s) 460, 586, 826, 1398, 1429, 1879, 2371, 2832
StyI CCWWGG 2 cut(s) 408, 2158
TaaI ACNGT 7 cut(s) 886, 1084, 1490, 2120, 2185, 2675, 2866
TaiI ACGT 2 cut(s) 63, 1336
TaqI TCGA 9 cut(s) 519, 1102, 1116, 1849, 1918, 2019, 2084, 2808, 2841
TatI WGTACW 4 cut(s) 758, 1867, 1906, 2309
TauI GCSGC 1 cut(s) 2288
TfiI GAWTC 5 cut(s) 703, 1113, 1303, 1516, 2810
Tru1I TTAA 9 cut(s) 480, 1019, 1062, 1503, 1533, 1782, 1800, 2058, 2730
Tru9I TTAA 9 cut(s) 480, 1019, 1062, 1503, 1533, 1782, 1800, 2058, 2730
TscAI CASTG 7 cut(s) 537, 601, 658, 1000, 1495, 2562, 2820
TseFI GTSAC 7 cut(s) 95, 564, 574, 895, 995, 1644, 2335
Tsp45I GTSAC 7 cut(s) 95, 564, 574, 895, 995, 1644, 2335
TspDTI ATGAA 8 cut(s) 650, 1331, 1438, 1952, 1981, 2023, 2240, 2533
TspGWI ACGGA 2 cut(s) 439, 1964
TspRI CASTG 7 cut(s) 537, 601, 658, 1000, 1495, 2562, 2820
Tth111I GACNNNGTC 1 cut(s) 1581
VneI GTGCAC 2 cut(s) 1196, 1284
XagI CCTNNNNNAGG 1 cut(s) 2605
XapI RAATTY 3 cut(s) 745, 864, 2231
XceI RCATGY 3 cut(s) 618, 1606, 2807
XcmI CCANNNNNNNNNTGG 1 cut(s) 1437
XhoI CTCGAG 1 cut(s) 1101
XmnI GAANNNNTTC 1 cut(s) 1855
XspI CTAG 8 cut(s) 845, 1328, 1385, 1479, 1914, 2129, 2171, 2226
Zsp2I ATGCAT 1 cut(s) 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.