AT3G25420

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002596
Physical Location & Seq
Forward (+)
9217832 .. 9220836
3005 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25420.1

Sequence Viewer

Length: 1485 bp
ATGGGTCGGTTAGTAGAAGCTATCATTGCTTCGATATTATTGTCTTTGTGTTTTACAATAACAAAGTCAGCTCCAAAATCTGCTCTCATTACCAATCTTCCTGGTTTCAACGGCACTTTTCCATCTAAACACTATGCCGGGTATGTGGCAATAGATAAACATCGTAATAAGAATCTATGGTATTACTTTGTGGAATCTGAGAGAAATGCTTCGGTAGATCCAGTTGTGCTTTGGCTTAATGGTGGTCCAGGTTGCTCTAGCATGGATGGATTTGTCTACGAGCACGGTCCATTCAATTTCGAACCAAAAAAGAAGAACTCACATCTTTTGCATCTCAATCCTTATAGTTGGTCCAAGGTGTCCAACATCATATACCTTGACTCTCCAGTTGGTGTGGGATTCTCTTACTCAAATGACAACGCCGATTATACAACTGATGATACAAAAACCGCGTCTGATACTCACACATTCCTTCTCGAGTGGTTTAAAATGTTTCCGGAGTTTCAGTCAAATCCGTTCTTTATATCAGGAGAATCATATGCTGGTATCTATGTGCCAACTCTTGCGGCCGAGGTTGTCAAAGGTCACAAGAATGTGACAAAGCCGGTTATCAACTTCAAGGGATATTTGGTGGGTAATGGTGTTACCGATGAGGTGTTTGATGGTAATGCTCTTGTTCCCTTCACTCATGGCATGGGACTCATCTCTGATGAACTCTATGAGGAAACTAAATTAGTGTGCAACGGAACATACTATACCGGCGGCCAGTCAGGAGTGAGTAAAGAATGTGCTGGCAAACTTAAAACAGTGTCAGATACTGTGAACTTATTGAACTTATATAACATTCTAGAACCGTGCTACCACGGAACATCTCTATCCGCCTTAGATATCGAGTTTCTGCCTAAGAGTCTCCTTACTCTAGGCAAAACAGAAAAGCCAATGGCGGTGAGAAAGAGAATGTTTGGTCGTGCATGGCCTCTAGGTGCCGTTGTGCGTCCAGGCATTGTACCTAGTTGGTCTCAACTCCTCGCTGGCTTTGGTGTTCCTTGCATTGACGATACAGTTGCGACCAAATGGCTAAACGATCCAGCTGTAAGGAAAGCGGTTCATGCTAAAGAGGAAAAAGCGATTGGAAATTGGGAGTTATGTTCAAGCAACCTCGAATATAGGCACGACACCGGTAGCATGATCGAATACCATAGAAACCTCACTCTTAGTGGCTTTCGAGCTCTCATTTTCAGTGGAGATCATGATATGTGTGTACCATATACTGGCTCTGAAGCATGGACTAAGGCAATGGGATACAAAGTGGTTGATGAATGGAGGCCATGGATGTCAAATAACCAAGTCGCCGGGTTTACACAAGGGTATGCCAATAATCTCACATTTCTAACCATCAAGGGTGCAGGACATACCGTTCCAGAATACAAACCGCGTGAATCTTTGGACTTCTATAGTCGTTTCCTAGCAGGAGAAAAGATATAG

Protein Analysis

494

Amino Acids

54.88

Weight (kDa)

6.54

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 34 - 490 1.9e-146 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 983
AccB7I CCANNNNNTGG 1 cut(s) 1271
AccI GTMKAC 1 cut(s) 276
AccII CGCG 2 cut(s) 452, 1435
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 7 cut(s) 450, 566, 762, 879, 944, 1103, 1433
AclWI GGATC 2 cut(s) 212, 1079
AcoI YGGCCR 2 cut(s) 567, 763
AcuI CTGAAG 1 cut(s) 1299
AfaI GTAC 2 cut(s) 1008, 1263
AfiI CCNNNNNNNGG 1 cut(s) 1271
AgeI ACCGGT 1 cut(s) 1178
AgsI TTSAA 5 cut(s) 109, 295, 619, 832, 1152
AjnI CCWGG 3 cut(s) 100, 247, 997
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
AloI GAACNNNNNNTCC 2 cut(s) 1401, 1433
AluBI AGCT 4 cut(s) 20, 71, 1091, 1229
AluI AGCT 4 cut(s) 20, 71, 1091, 1229
Alw21I GWGCWC 2 cut(s) 285, 1231
Alw26I GTCTC 2 cut(s) 914, 1023
AlwI GGATC 2 cut(s) 212, 1079
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 476
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 4 cut(s) 567, 763, 974, 1325
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
AsiGI ACCGGT 1 cut(s) 1178
Asp700I GAANNNNTTC 1 cut(s) 208
AspS9I GGNCC 3 cut(s) 245, 287, 351
AsuC2I CCSGG 2 cut(s) 139, 1354
AsuHPI GGTGA 1 cut(s) 958
AsuII TTCGAA 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 3 cut(s) 245, 287, 351
BanI GGYRCC 1 cut(s) 983
BanII GRGCYC 1 cut(s) 1231
Bbv12I GWGCWC 2 cut(s) 285, 1231
BccI CCATC 4 cut(s) 130, 260, 656, 1403
BceAI ACGGC 2 cut(s) 127, 971
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 3 cut(s) 102, 249, 999
BciVI GTATCC 1 cut(s) 1295
BcnI CCSGG 2 cut(s) 139, 1354
BcoDI GTCTC 2 cut(s) 914, 1023
BfaI CTAG 6 cut(s) 258, 848, 920, 980, 1011, 1466
BfmI CTRYAG 1 cut(s) 1453
BfuI GTATCC 1 cut(s) 1295
BisI GCNGC 2 cut(s) 567, 763
BlsI GCNGC 2 cut(s) 568, 764
Bme1390I CCNGG 5 cut(s) 102, 139, 249, 999, 1354
Bme18I GGWCC 3 cut(s) 245, 287, 351
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 3 cut(s) 245, 287, 351
BmiI GGNNCC 1 cut(s) 985
BmrFI CCNGG 5 cut(s) 102, 139, 249, 999, 1354
BmsI GCATC 1 cut(s) 340
BpmI CTGGAG 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 300
BpuMI CCSGG 2 cut(s) 139, 1354
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 1023
BsaJI CCNNGG 4 cut(s) 354, 570, 862, 1328
BsaWI WCCGGW 2 cut(s) 496, 1178
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 1271
Bse118I RCCGGY 3 cut(s) 604, 758, 1178
Bse1I ACTGG 4 cut(s) 221, 386, 766, 1276
Bse3DI GCAATG 2 cut(s) 24, 1302
Bse8I GATNNNNATC 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 3 cut(s) 102, 249, 999
BseDI CCNNGG 4 cut(s) 354, 570, 862, 1328
BseGI GGATG 2 cut(s) 271, 1338
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 1271
BseMI GCAATG 2 cut(s) 24, 1302
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 4 cut(s) 221, 386, 766, 1276
BseRI GAGGAG 1 cut(s) 1016
BseX3I CGGCCG 1 cut(s) 567
BsgI GTGCAG 1 cut(s) 1425
Bsh1236I CGCG 2 cut(s) 452, 1435
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 4 cut(s) 569, 765, 976, 1327
BshNI GGYRCC 1 cut(s) 983
BshTI ACCGGT 1 cut(s) 1178
BsiEI CGRYCG 1 cut(s) 570
BsiHKAI GWGCWC 2 cut(s) 285, 1231
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 6 cut(s) 138, 497, 605, 759, 1179, 1353
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 1 cut(s) 1271
BsmAI GTCTC 2 cut(s) 914, 1023
BsmFI GGGAC 1 cut(s) 711
BsnI GGCC 4 cut(s) 569, 765, 976, 1327
Bso31I GGTCTC 1 cut(s) 1023
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 285, 1231
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 4 cut(s) 217, 1084, 1188, 1246
Bsp19I CCATGG 1 cut(s) 1328
BspACI CCGC 7 cut(s) 450, 566, 762, 879, 944, 1103, 1433
BspANI GGCC 4 cut(s) 569, 765, 976, 1327
BspCNI CTCAG 1 cut(s) 190
BspEI TCCGGA 1 cut(s) 496
BspFNI CGCG 2 cut(s) 452, 1435
BspHI TCATGA 1 cut(s) 1249
BspLI GGNNCC 1 cut(s) 985
BspPI GGATC 2 cut(s) 212, 1079
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 983
BspTNI GGTCTC 1 cut(s) 1023
BsrDI GCAATG 2 cut(s) 24, 1302
BsrFI RCCGGY 3 cut(s) 604, 758, 1178
BsrI ACTGG 4 cut(s) 221, 386, 766, 1276
BssAI RCCGGY 3 cut(s) 604, 758, 1178
BssECI CCNNGG 4 cut(s) 354, 570, 862, 1328
BssMI GATC 4 cut(s) 217, 1084, 1188, 1246
BssT1I CCWWGG 2 cut(s) 354, 1328
Bst2UI CCWGG 3 cut(s) 102, 249, 999
Bst4CI ACNGT 6 cut(s) 287, 808, 820, 855, 1063, 1417
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 793, 1033
BstDEI CTNAG 5 cut(s) 198, 883, 903, 1214, 1290
BstDSI CCRYGG 2 cut(s) 862, 1328
BstF5I GGATG 2 cut(s) 271, 1338
BstFNI CGCG 2 cut(s) 452, 1435
BstKTI GATC 4 cut(s) 220, 1087, 1191, 1249
BstMAI GTCTC 2 cut(s) 914, 1023
BstMBI GATC 4 cut(s) 217, 1084, 1188, 1246
BstMCI CGRYCG 1 cut(s) 570
BstMWI GCNNNNNNNGC 2 cut(s) 26, 1109
BstNI CCWGG 3 cut(s) 102, 249, 999
BstSCI CCNGG 5 cut(s) 100, 137, 247, 997, 1352
BstSFI CTRYAG 1 cut(s) 1453
BstUI CGCG 2 cut(s) 452, 1435
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BstZI CGGCCG 1 cut(s) 567
BsuI GTATCC 1 cut(s) 1295
BsuRI GGCC 4 cut(s) 569, 765, 976, 1327
BtgI CCRYGG 2 cut(s) 862, 1328
BtsCI GGATG 2 cut(s) 271, 1338
BtsIMutI CAGTG 2 cut(s) 813, 1246
Cac8I GCNNGC 2 cut(s) 793, 1033
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 1249
Cfr10I RCCGGY 3 cut(s) 604, 758, 1178
Cfr13I GGNCC 3 cut(s) 245, 287, 351
CseI GACGC 2 cut(s) 441, 983
Csp6I GTAC 2 cut(s) 1007, 1262
CspAI ACCGGT 1 cut(s) 1178
CviAII CATG 9 cut(s) 262, 689, 694, 972, 1109, 1186, 1250, 1284, 1329
CviQI GTAC 2 cut(s) 1007, 1262
DdeI CTNAG 5 cut(s) 198, 883, 903, 1214, 1290
DpnI GATC 4 cut(s) 219, 1086, 1190, 1248
DpnII GATC 4 cut(s) 217, 1084, 1188, 1246
DraI TTTAAA 1 cut(s) 487
EaeI YGGCCR 2 cut(s) 567, 763
EagI CGGCCG 1 cut(s) 567
EciI GGCGGA 1 cut(s) 868
Ecl136II GAGCTC 1 cut(s) 1229
EclXI CGGCCG 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 354, 1328
Eco24I GRGCYC 1 cut(s) 1231
Eco31I GGTCTC 1 cut(s) 1023
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 3 cut(s) 245, 287, 351
Eco52I CGGCCG 1 cut(s) 567
Eco53kI GAGCTC 1 cut(s) 1229
Eco57I CTGAAG 1 cut(s) 1299
Eco88I CYCGRG 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 1229
EcoRII CCWGG 3 cut(s) 100, 247, 997
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 2 cut(s) 354, 1328
EcoT38I GRGCYC 1 cut(s) 1231
ErhI CCWWGG 2 cut(s) 354, 1328
FaeI CATG 9 cut(s) 265, 692, 697, 975, 1112, 1189, 1253, 1287, 1332
FaqI GGGAC 1 cut(s) 711
FatI CATG 9 cut(s) 261, 688, 693, 971, 1108, 1185, 1249, 1283, 1328
FauNDI CATATG 1 cut(s) 538
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 567, 763
FokI GGATG 2 cut(s) 278, 1345
FriOI GRGCYC 1 cut(s) 1231
Fsp4HI GCNGC 2 cut(s) 567, 763
FspBI CTAG 6 cut(s) 258, 848, 920, 980, 1011, 1466
GluI GCNGC 2 cut(s) 567, 763
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 4 cut(s) 569, 765, 976, 1327
HapII CCGG 6 cut(s) 138, 497, 605, 759, 1179, 1353
HgaI GACGC 2 cut(s) 441, 983
Hin1II CATG 9 cut(s) 265, 692, 697, 975, 1112, 1189, 1253, 1287, 1332
HinfI GANTC 8 cut(s) 172, 194, 380, 399, 533, 699, 907, 1439
HpaII CCGG 6 cut(s) 138, 497, 605, 759, 1179, 1353
HphI GGTGA 1 cut(s) 958
Hpy166II GTNNAC 4 cut(s) 277, 823, 1262, 1359
Hpy188I TCNGA 5 cut(s) 199, 457, 709, 814, 1279
Hpy188III TCNNGA 7 cut(s) 476, 497, 528, 771, 848, 1250, 1421
Hpy8I GTNNAC 4 cut(s) 277, 823, 1262, 1359
HpyAV CCTTC 2 cut(s) 482, 691
HpyCH4III ACNGT 6 cut(s) 287, 808, 820, 855, 1063, 1417
HpyCH4V TGCA 5 cut(s) 331, 741, 971, 1050, 1406
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 1109
HpyF3I CTNAG 5 cut(s) 198, 883, 903, 1214, 1290
Hsp92II CATG 9 cut(s) 265, 692, 697, 975, 1112, 1189, 1253, 1287, 1332
Kpn2I TCCGGA 1 cut(s) 496
Kzo9I GATC 4 cut(s) 217, 1084, 1188, 1246
LmnI GCTCC 1 cut(s) 76
LweI GCATC 1 cut(s) 340
MaeI CTAG 6 cut(s) 258, 848, 920, 980, 1011, 1466
MaeIII GTNAC 3 cut(s) 584, 595, 643
MalI GATC 4 cut(s) 219, 1086, 1190, 1248
MboI GATC 4 cut(s) 217, 1084, 1188, 1246
MboII GAAGA 2 cut(s) 89, 325
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 285, 1231
MluCI AATT 3 cut(s) 295, 731, 1135
MlyI GAGTC 3 cut(s) 374, 693, 916
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 9 cut(s) 565, 646, 715, 987, 1037, 1111, 1169, 1217, 1317
MroI TCCGGA 1 cut(s) 496
MroXI GAANNNNTTC 1 cut(s) 208
MseI TTAA 3 cut(s) 237, 486, 801
MslI CAYNNNNRTG 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 1091
MspI CCGG 6 cut(s) 138, 497, 605, 759, 1179, 1353
MspR9I CCNGG 5 cut(s) 102, 139, 249, 999, 1354
MvaI CCWGG 3 cut(s) 102, 249, 999
MvnI CGCG 2 cut(s) 452, 1435
MwoI GCNNNNNNNGC 2 cut(s) 26, 1109
NciI CCSGG 2 cut(s) 139, 1354
NcoI CCATGG 1 cut(s) 1328
NdeI CATATG 1 cut(s) 538
NdeII GATC 4 cut(s) 217, 1084, 1188, 1246
NlaIII CATG 9 cut(s) 265, 692, 697, 975, 1112, 1189, 1253, 1287, 1332
NlaIV GGNNCC 1 cut(s) 985
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 2 cut(s) 584, 595
NspV TTCGAA 1 cut(s) 300
PaeR7I CTCGAG 1 cut(s) 476
PagI TCATGA 1 cut(s) 1249
PdmI GAANNNNTTC 1 cut(s) 208
PfeI GAWTC 5 cut(s) 172, 194, 399, 533, 1439
PflMI CCANNNNNTGG 1 cut(s) 1271
PinAI ACCGGT 1 cut(s) 1178
PkrI GCNGC 2 cut(s) 568, 764
PleI GAGTC 3 cut(s) 374, 693, 915
PpsI GAGTC 3 cut(s) 374, 693, 915
Psp124BI GAGCTC 1 cut(s) 1231
Psp6I CCWGG 3 cut(s) 100, 247, 997
PspGI CCWGG 3 cut(s) 100, 247, 997
PspN4I GGNNCC 1 cut(s) 985
PspPI GGNCC 3 cut(s) 245, 287, 351
PsrI GAACNNNNNNTAC 2 cut(s) 739, 771
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 217
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 2 cut(s) 1008, 1263
RsaNI GTAC 2 cut(s) 1007, 1262
RseI CAYNNNNRTG 1 cut(s) 591
SacI GAGCTC 1 cut(s) 1231
SaqAI TTAA 3 cut(s) 237, 486, 801
SatI GCNGC 2 cut(s) 567, 763
Sau3AI GATC 4 cut(s) 217, 1084, 1188, 1246
Sau96I GGNCC 3 cut(s) 245, 287, 351
SchI GAGTC 3 cut(s) 374, 693, 916
ScrFI CCNGG 5 cut(s) 102, 139, 249, 999, 1354
SduI GDGCHC 2 cut(s) 285, 1231
SfaNI GCATC 1 cut(s) 340
SfcI CTRYAG 1 cut(s) 1453
Sfr274I CTCGAG 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 3 cut(s) 245, 287, 351
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 3 cut(s) 295, 731, 1135
SsiI CCGC 7 cut(s) 450, 566, 762, 879, 944, 1103, 1433
SspMI CTAG 6 cut(s) 258, 848, 920, 980, 1011, 1466
SstI GAGCTC 1 cut(s) 1231
StyD4I CCNGG 5 cut(s) 100, 137, 247, 997, 1352
StyI CCWWGG 2 cut(s) 354, 1328
TaaI ACNGT 6 cut(s) 287, 808, 820, 855, 1063, 1417
TaqI TCGA 7 cut(s) 32, 300, 477, 891, 1161, 1191, 1225
TasI AATT 3 cut(s) 295, 731, 1135
TauI GCSGC 2 cut(s) 569, 765
TfiI GAWTC 5 cut(s) 172, 194, 399, 533, 1439
Tru1I TTAA 3 cut(s) 237, 486, 801
Tru9I TTAA 3 cut(s) 237, 486, 801
TscAI CASTG 2 cut(s) 813, 1246
TseFI GTSAC 2 cut(s) 584, 595
Tsp45I GTSAC 2 cut(s) 584, 595
TspDTI ATGAA 3 cut(s) 726, 1097, 1332
TspGWI ACGGA 3 cut(s) 504, 759, 879
TspRI CASTG 2 cut(s) 813, 1246
Van91I CCANNNNNTGG 1 cut(s) 1271
VpaK11BI GGWCC 3 cut(s) 245, 287, 351
XbaI TCTAGA 1 cut(s) 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 228
XhoI CTCGAG 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 208
XspI CTAG 6 cut(s) 258, 848, 920, 980, 1011, 1466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.