Rroxscaffold_6G00425950

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002596
Physical Location & Seq
Forward (+)
46883481 .. 46887611
4131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00425950.1

Sequence Viewer

Length: 1524 bp
ATGGCGGGCATGGCCAGACCGTTGGCTACAATTCCCACCTGCTTCTCAATCATAATCATATGCTTAAGCTTGATAGCAATGAGAATTGAGGCAGCCCCTCAAGGCTCTCTTGTCACGCAGCTTCCCGGCTTCAATGGCACTTTTCCGTCCAGCCACTATTCCGGATATGTAAGCATAGGCGCAAAGAATCTTTTCTACTACTTCGTAGTGTCAGAAGGGAGCCCCGCAGACGATCCTGTTGTCTTGTGGCTCAATGGCGGACCTGGCTGCTCTAGCTTTGACGGATTCGTCTACGAACACGGGCCGTTCAATTTTGAACAAAAATCGAAAGGAGCATTGCCCACTTTACAACTTAATCCTTACAGCTGGTCCAAGGTTTCCAACATGCTGTATTTGGATTCTCCTGCTGGTGTTGGGTTTTCCTTCTCAGAAGACCCAAGCCAATACGAAACCGGGGATCTTCAAACTGCTGCCGACACCCACGCCTTCCTCCTCAGCTGGTTTAAGCAGTACCCGGAGTTTCTTTCCAATCCCTTTTACATTTCCGGGGAGTCTTATGCTGGAGTCTACGTCCCTACTCTTGCCTTTCAAGTTGCCACAGGTATTCAAAATGGAACAAAACCAGTTGTAAATCTCAAGGGTTACATGGTGGGGAATGGAGTCACAGACAGAAACTTTGATGACAATGCTCTTGTCCCTTTTGCTCATGGGATGGCCCTCATTTCAGATCAAATTTATGAGGGAGTTATAGAAGAATGTGGGATCAACTACTCCAACTCATCTAGTCCCAAGTGCAATGAGAAACTTAATCTGGCTCGCGGGGCTCTTAGTGGACTAAATATCTACAACGTCCTTGAGCCTTGTTACCACAACCCTGGAGCTGAATCAGCAAATGATACTTCTACAACAACCAAAAGCAGCAACTTACCCTTTAGCTTTCAGCAACTAGGCAAGTCGCCTGAGAAGCCTCTCGGAGTGAGAAAGAGGATGTTTGGCCGCGCTTGGCCTTATCAGATGTCTGTAGAAGATGGCTTAGTCCCTTTGTGGCCACAGCTAGCTGCTAAATCTAACTCACGCAGTGTTCCATGCTTTAATGATGAAGTCGCAACTAAATGGCTGAACGACCCCGCAGTTAGGGAAGCAATTCATGCCAAGCCTGCAAGTGTGTCTGGTCCCTGGGAATTATGCTCGGATAGAATAAGTTATTATCATGATGCTGGAAGTATGATCCCTTACCACCGAAACCTTACAACCAAAGGATACAAAGCAATCATTTTCAGTGGAGACCATGATATGTGTGTGCCATATACTGGAAGCCAGGCATGGACTAGCTCACTTGGGTATAAGGTTATAGACCAATGGAGGTCTTGGCTATCCAATGACCAAGTTGCAGGCTACTTGCAAGCATATGAAAACGACCTCACCTTTCTCACTGTCAAGGGAGCTGGACACACCGTCCCTGAATACAAGCCACGGGAGGCACTCGACTTCTACAGCCGCTGGTTGGCAGGAAAACCGATATGA

Protein Analysis

507

Amino Acids

55.71

Weight (kDa)

5.61

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 42 - 503 4.7e-135 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
AasI GACNNNNNNGTC 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 47
AccB7I CCANNNNNTGG 1 cut(s) 1310
AccI GTMKAC 2 cut(s) 291, 567
AccII CGCG 2 cut(s) 819, 999
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 7 cut(s) 5, 225, 258, 819, 997, 1128, 1498
AclWI GGATC 4 cut(s) 227, 465, 770, 1222
AcoI YGGCCR 3 cut(s) 12, 994, 1046
AcsI RAATTY 1 cut(s) 732
AdeI CACNNNGTG 1 cut(s) 1079
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 3 cut(s) 1134, 1310, 1504
AflII CTTAAG 1 cut(s) 64
AgsI TTSAA 6 cut(s) 133, 310, 317, 464, 590, 608
AhdI GACNNNNNGTC 1 cut(s) 1454
AjnI CCWGG 4 cut(s) 262, 874, 1175, 1317
Alw26I GTCTC 1 cut(s) 1278
AlwI GGATC 4 cut(s) 227, 465, 770, 1222
AlwNI CAGNNNCTG 1 cut(s) 1500
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 6 cut(s) 12, 302, 714, 994, 1004, 1046
ApeKI GCWGC 6 cut(s) 92, 118, 267, 470, 918, 1058
ApoI RAATTY 1 cut(s) 732
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
Asp700I GAANNNNTTC 2 cut(s) 191, 1143
AspLEI GCGC 2 cut(s) 182, 1001
AspS9I GGNCC 5 cut(s) 260, 302, 369, 715, 1172
AsuC2I CCSGG 4 cut(s) 126, 454, 515, 547
AsuHPI GGTGA 1 cut(s) 1414
AsuNHI GCTAGC 1 cut(s) 1054
AvaII GGWCC 3 cut(s) 260, 369, 1172
BalI TGGCCA 2 cut(s) 14, 1048
BanII GRGCYC 2 cut(s) 224, 826
BbsI GAAGAC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 6 cut(s) 104, 130, 254, 457, 930, 1045
BccI CCATC 2 cut(s) 706, 1022
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 4 cut(s) 264, 876, 1177, 1319
BciVI GTATCC 1 cut(s) 1253
BcnI CCSGG 4 cut(s) 126, 454, 515, 547
BcoDI GTCTC 1 cut(s) 1278
BfaI CTAG 5 cut(s) 273, 783, 947, 1055, 1329
BfmI CTRYAG 2 cut(s) 1020, 1492
BfrI CTTAAG 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 47
BfuI GTATCC 1 cut(s) 1253
BisI GCNGC 8 cut(s) 93, 119, 268, 471, 919, 997, 1059, 1498
BlsI GCNGC 8 cut(s) 94, 120, 269, 472, 920, 998, 1060, 1499
Bme1390I CCNGG 8 cut(s) 126, 264, 454, 515, 547, 876, 1177, 1319
Bme18I GGWCC 3 cut(s) 260, 369, 1172
BmeRI GACNNNNNGTC 1 cut(s) 1454
BmgT120I GGNCC 5 cut(s) 260, 302, 369, 715, 1172
BmiI GGNNCC 2 cut(s) 221, 1174
BmrFI CCNGG 8 cut(s) 126, 264, 454, 515, 547, 876, 1177, 1319
BmsI GCATC 1 cut(s) 1204
BmtI GCTAGC 1 cut(s) 1058
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 2 cut(s) 582, 897
Bpu10I CCTNAGC 1 cut(s) 494
BpuEI CTTGAG 3 cut(s) 84, 620, 875
BpuMI CCSGG 4 cut(s) 126, 454, 515, 547
BsaI GGTCTC 1 cut(s) 1278
BsaJI CCNNGG 7 cut(s) 372, 453, 546, 874, 1175, 1176, 1472
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 3 cut(s) 1134, 1310, 1504
Bse1I ACTGG 2 cut(s) 623, 1315
Bse3DI GCAATG 3 cut(s) 84, 335, 802
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 4 cut(s) 264, 876, 1177, 1319
BseDI CCNNGG 7 cut(s) 372, 453, 546, 874, 1175, 1176, 1472
BseGI GGATG 2 cut(s) 717, 993
BseLI CCNNNNNNNGG 3 cut(s) 1134, 1310, 1504
BseMI GCAATG 3 cut(s) 84, 335, 802
BseMII CTCAG 3 cut(s) 441, 508, 951
BseNI ACTGG 2 cut(s) 623, 1315
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 6 cut(s) 104, 130, 254, 457, 930, 1045
Bsh1236I CGCG 2 cut(s) 819, 999
BshFI GGCC 6 cut(s) 14, 304, 716, 996, 1006, 1048
BsiSI CCGG 5 cut(s) 126, 162, 453, 515, 546
BslFI GGGAC 6 cut(s) 557, 680, 771, 1022, 1158, 1442
BslI CCNNNNNNNGG 3 cut(s) 1134, 1310, 1504
BsmAI GTCTC 1 cut(s) 1278
BsmFI GGGAC 6 cut(s) 557, 680, 771, 1022, 1158, 1442
BsnI GGCC 6 cut(s) 14, 304, 716, 996, 1006, 1048
Bso31I GGTCTC 1 cut(s) 1278
Bsp1286I GDGCHC 2 cut(s) 224, 826
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 5 cut(s) 232, 457, 727, 762, 1227
BspACI CCGC 7 cut(s) 5, 225, 258, 819, 997, 1128, 1498
BspANI GGCC 6 cut(s) 14, 304, 716, 996, 1006, 1048
BspCNI CTCAG 3 cut(s) 440, 507, 952
BspEI TCCGGA 1 cut(s) 161
BspFNI CGCG 2 cut(s) 819, 999
BspHI TCATGA 1 cut(s) 1210
BspLI GGNNCC 2 cut(s) 221, 1174
BspMI ACCTGC 1 cut(s) 47
BspOI GCTAGC 1 cut(s) 1058
BspPI GGATC 4 cut(s) 227, 465, 770, 1222
BspTI CTTAAG 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 1278
BsrDI GCAATG 3 cut(s) 84, 335, 802
BsrI ACTGG 2 cut(s) 623, 1315
BssECI CCNNGG 7 cut(s) 372, 453, 546, 874, 1175, 1176, 1472
BssMI GATC 5 cut(s) 232, 457, 727, 762, 1227
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 4 cut(s) 264, 876, 1177, 1319
Bst4CI ACNGT 3 cut(s) 21, 1435, 1456
BstAFI CTTAAG 1 cut(s) 64
BstAPI GCANNNNNTGC 1 cut(s) 1148
BstC8I GCNNGC 6 cut(s) 7, 817, 1056, 1158, 1393, 1404
BstDEI CTNAG 5 cut(s) 427, 494, 827, 960, 1033
BstDSI CCRYGG 1 cut(s) 1472
BstF5I GGATG 2 cut(s) 717, 993
BstFNI CGCG 2 cut(s) 819, 999
BstHHI GCGC 2 cut(s) 182, 1001
BstKTI GATC 5 cut(s) 235, 460, 730, 765, 1230
BstMAI GTCTC 1 cut(s) 1278
BstMBI GATC 5 cut(s) 232, 457, 727, 762, 1227
BstMWI GCNNNNNNNGC 9 cut(s) 11, 135, 264, 273, 821, 887, 964, 1148, 1157
BstNI CCWGG 4 cut(s) 264, 876, 1177, 1319
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 8 cut(s) 124, 262, 452, 513, 545, 874, 1175, 1317
BstSFI CTRYAG 2 cut(s) 1020, 1492
BstUI CGCG 2 cut(s) 819, 999
BstV1I GCAGC 6 cut(s) 104, 130, 254, 457, 930, 1045
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 457
BstXI CCANNNNNNTGG 2 cut(s) 22, 875
BstYI RGATCY 1 cut(s) 457
BsuI GTATCC 1 cut(s) 1253
BsuRI GGCC 6 cut(s) 14, 304, 716, 996, 1006, 1048
BtgI CCRYGG 1 cut(s) 1472
BtsCI GGATG 2 cut(s) 717, 993
BtsI GCAGTG 1 cut(s) 1084
BtsIMutI CAGTG 3 cut(s) 1084, 1285, 1431
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 6 cut(s) 7, 817, 1056, 1158, 1393, 1404
CaiI CAGNNNCTG 1 cut(s) 1500
CciI TCATGA 1 cut(s) 1210
CfoI GCGC 2 cut(s) 182, 1001
Cfr13I GGNCC 5 cut(s) 260, 302, 369, 715, 1172
Csp6I GTAC 1 cut(s) 511
CviAII CATG 9 cut(s) 10, 385, 646, 707, 1086, 1148, 1211, 1289, 1323
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 5 cut(s) 427, 494, 827, 960, 1033
DpnI GATC 5 cut(s) 234, 459, 729, 764, 1229
DpnII GATC 5 cut(s) 232, 457, 727, 762, 1227
DraIII CACNNNGTG 1 cut(s) 1079
DrdI GACNNNNNNGTC 1 cut(s) 287
DriI GACNNNNNGTC 1 cut(s) 1454
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 3 cut(s) 12, 994, 1046
Eam1105I GACNNNNNGTC 1 cut(s) 1454
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 224, 826
Eco31I GGTCTC 1 cut(s) 1278
Eco47I GGWCC 3 cut(s) 260, 369, 1172
EcoRII CCWGG 4 cut(s) 262, 874, 1175, 1317
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 2 cut(s) 224, 826
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 9 cut(s) 13, 388, 649, 710, 1089, 1151, 1214, 1292, 1326
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 6 cut(s) 557, 680, 771, 1022, 1158, 1442
FatI CATG 9 cut(s) 9, 384, 645, 706, 1085, 1147, 1210, 1288, 1322
FauI CCCGC 3 cut(s) 232, 812, 1135
FauNDI CATATG 2 cut(s) 59, 1408
FblI GTMKAC 2 cut(s) 291, 567
Fnu4HI GCNGC 8 cut(s) 93, 119, 268, 471, 919, 997, 1059, 1498
FokI GGATG 2 cut(s) 724, 1000
FriOI GRGCYC 2 cut(s) 224, 826
Fsp4HI GCNGC 8 cut(s) 93, 119, 268, 471, 919, 997, 1059, 1498
FspBI CTAG 5 cut(s) 273, 783, 947, 1055, 1329
GlaI GCGC 2 cut(s) 181, 1000
GluI GCNGC 8 cut(s) 93, 119, 268, 471, 919, 997, 1059, 1498
GsuI CTGGAG 2 cut(s) 582, 897
HaeIII GGCC 6 cut(s) 14, 304, 716, 996, 1006, 1048
HapII CCGG 5 cut(s) 126, 162, 453, 515, 546
HhaI GCGC 2 cut(s) 182, 1001
Hin1II CATG 9 cut(s) 13, 388, 649, 710, 1089, 1151, 1214, 1292, 1326
Hin6I GCGC 2 cut(s) 180, 999
HinP1I GCGC 2 cut(s) 180, 999
HindIII AAGCTT 1 cut(s) 67
HinfI GANTC 7 cut(s) 187, 285, 398, 551, 564, 660, 884
HpaII CCGG 5 cut(s) 126, 162, 453, 515, 546
HphI GGTGA 1 cut(s) 1414
Hpy166II GTNNAC 3 cut(s) 292, 568, 833
Hpy188I TCNGA 6 cut(s) 214, 430, 727, 974, 1014, 1192
Hpy188III TCNNGA 2 cut(s) 162, 1211
Hpy8I GTNNAC 3 cut(s) 292, 568, 833
HpyAV CCTTC 3 cut(s) 209, 433, 496
HpyCH4III ACNGT 3 cut(s) 21, 1435, 1456
HpyCH4IV ACGT 2 cut(s) 570, 849
HpyCH4V TGCA 4 cut(s) 795, 1160, 1391, 1402
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 135, 264, 273, 821, 887, 964, 1148, 1157
HpyF3I CTNAG 5 cut(s) 427, 494, 827, 960, 1033
HpySE526I ACGT 2 cut(s) 570, 849
Hsp92II CATG 9 cut(s) 13, 388, 649, 710, 1089, 1151, 1214, 1292, 1326
HspAI GCGC 2 cut(s) 180, 999
Kpn2I TCCGGA 1 cut(s) 161
Kzo9I GATC 5 cut(s) 232, 457, 727, 762, 1227
LmnI GCTCC 4 cut(s) 219, 332, 878, 1442
Lsp1109I GCAGC 6 cut(s) 104, 130, 254, 457, 930, 1045
LweI GCATC 1 cut(s) 1204
MaeI CTAG 5 cut(s) 273, 783, 947, 1055, 1329
MaeII ACGT 2 cut(s) 570, 849
MaeIII GTNAC 4 cut(s) 112, 641, 661, 863
MalI GATC 5 cut(s) 234, 459, 729, 764, 1229
MboI GATC 5 cut(s) 232, 457, 727, 762, 1227
MboII GAAGA 4 cut(s) 443, 452, 764, 1037
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 2 cut(s) 224, 826
MlsI TGGCCA 2 cut(s) 14, 1048
MluCI AATT 6 cut(s) 30, 84, 310, 732, 1143, 1181
MluNI TGGCCA 2 cut(s) 14, 1048
MlyI GAGTC 3 cut(s) 560, 573, 669
MmeI TCCRAC 2 cut(s) 405, 798
Mox20I TGGCCA 2 cut(s) 14, 1048
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 2 cut(s) 191, 1143
MscI TGGCCA 2 cut(s) 14, 1048
MseI TTAA 5 cut(s) 65, 354, 504, 807, 1092
Msp20I TGGCCA 2 cut(s) 14, 1048
MspA1I CMGCKG 3 cut(s) 366, 498, 1500
MspCI CTTAAG 1 cut(s) 64
MspI CCGG 5 cut(s) 126, 162, 453, 515, 546
MspR9I CCNGG 8 cut(s) 126, 264, 454, 515, 547, 876, 1177, 1319
MvaI CCWGG 4 cut(s) 264, 876, 1177, 1319
MvnI CGCG 2 cut(s) 819, 999
MwoI GCNNNNNNNGC 9 cut(s) 11, 135, 264, 273, 821, 887, 964, 1148, 1157
NciI CCSGG 4 cut(s) 126, 454, 515, 547
NdeI CATATG 2 cut(s) 59, 1408
NdeII GATC 5 cut(s) 232, 457, 727, 762, 1227
NheI GCTAGC 1 cut(s) 1054
NlaIII CATG 9 cut(s) 13, 388, 649, 710, 1089, 1151, 1214, 1292, 1326
NlaIV GGNNCC 2 cut(s) 221, 1174
NmuCI GTSAC 2 cut(s) 112, 661
NspI RCATGY 1 cut(s) 388
PagI TCATGA 1 cut(s) 1210
PaqCI CACCTGC 1 cut(s) 47
PasI CCCWGGG 1 cut(s) 1176
PdmI GAANNNNTTC 2 cut(s) 191, 1143
PfeI GAWTC 4 cut(s) 187, 285, 398, 884
PflMI CCANNNNNTGG 1 cut(s) 1310
PkrI GCNGC 8 cut(s) 94, 120, 269, 472, 920, 998, 1060, 1499
PleI GAGTC 3 cut(s) 559, 572, 668
PpsI GAGTC 3 cut(s) 559, 572, 668
Psp6I CCWGG 4 cut(s) 262, 874, 1175, 1317
PspGI CCWGG 4 cut(s) 262, 874, 1175, 1317
PspN4I GGNNCC 2 cut(s) 221, 1174
PspPI GGNCC 5 cut(s) 260, 302, 369, 715, 1172
PstNI CAGNNNCTG 1 cut(s) 1500
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 2 cut(s) 366, 498
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SaqAI TTAA 5 cut(s) 65, 354, 504, 807, 1092
SatI GCNGC 8 cut(s) 93, 119, 268, 471, 919, 997, 1059, 1498
Sau3AI GATC 5 cut(s) 232, 457, 727, 762, 1227
Sau96I GGNCC 5 cut(s) 260, 302, 369, 715, 1172
SchI GAGTC 3 cut(s) 560, 573, 669
ScrFI CCNGG 8 cut(s) 126, 264, 454, 515, 547, 876, 1177, 1319
SduI GDGCHC 2 cut(s) 224, 826
SfaNI GCATC 1 cut(s) 1204
SfcI CTRYAG 2 cut(s) 1020, 1492
SinI GGWCC 3 cut(s) 260, 369, 1172
SmlI CTYRAG 4 cut(s) 64, 99, 635, 854
SmoI CTYRAG 4 cut(s) 64, 99, 635, 854
Sse9I AATT 6 cut(s) 30, 84, 310, 732, 1143, 1181
SsiI CCGC 7 cut(s) 5, 225, 258, 819, 997, 1128, 1498
SspMI CTAG 5 cut(s) 273, 783, 947, 1055, 1329
StyD4I CCNGG 8 cut(s) 124, 262, 452, 513, 545, 874, 1175, 1317
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 3 cut(s) 21, 1435, 1456
TaiI ACGT 2 cut(s) 573, 852
TaqI TCGA 2 cut(s) 326, 1485
TasI AATT 6 cut(s) 30, 84, 310, 732, 1143, 1181
TauI GCSGC 2 cut(s) 999, 1500
TfiI GAWTC 4 cut(s) 187, 285, 398, 884
Tru1I TTAA 5 cut(s) 65, 354, 504, 807, 1092
Tru9I TTAA 5 cut(s) 65, 354, 504, 807, 1092
TscAI CASTG 3 cut(s) 1084, 1285, 1438
TseFI GTSAC 2 cut(s) 112, 661
TseI GCWGC 6 cut(s) 92, 118, 267, 470, 918, 1058
Tsp45I GTSAC 2 cut(s) 112, 661
TspDTI ATGAA 3 cut(s) 1113, 1136, 1425
TspGWI ACGGA 2 cut(s) 135, 297
TspRI CASTG 3 cut(s) 1084, 1285, 1438
Van91I CCANNNNNTGG 1 cut(s) 1310
Vha464I CTTAAG 1 cut(s) 64
VpaK11BI GGWCC 3 cut(s) 260, 369, 1172
XapI RAATTY 1 cut(s) 732
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 2 cut(s) 291, 567
XmnI GAANNNNTTC 2 cut(s) 191, 1143
XspI CTAG 5 cut(s) 273, 783, 947, 1055, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.