MD05G1158200.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002596
Physical Location & Seq
Reverse (-)
28737671 .. 28741235
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1158200.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGGGCGGGAGAGTATTATCTTTATTTTTGGTGCCATTAATCTACATCTTCTTGAGCTTTTTGCCAACCCAATCAGCTCCTCAATCTGCTCTCATAACCCAGATTCCTGGATTCAGTGGCACTCTTCCTTCAAAACACTATTCTGGGTATGTGACAGTTAACCAGAGTCATGGGAGGAACCTGTTCTACTATTTTGTCGAATCGGAAGGGAAGCCTGCGGAGGATCCGGTGGTTCTGTGGCTGAATGGTGGACCTGGTTGCTCCAGCTTTGATGGCTTTGTTTATGAGCATGGGCCATTCAATTTTGAAGAGGCCAAGATCAAGGGAAGTCTGCCTCAGCTCCACCTTAACCCATACAGCTGGTCAAAGGTTTCCAACATTATATATCTGGATTCTCCGGCTGGTGTTGGATTGTCTTACTCAAAAAATGAGACCGACTATAAAACTGGGGATCTAAAGACTGCCTCTGATTCCCACACATTTCTCCTCAAGTGGTTTGAACTGTACCCTGAGTTCCTCTCCAACCCATTTTTTATTGCTGGGGAGTCATATGCTGGAGTCTACGTGCCAACGCTTTCTTCTGAAGTAGTGAAAGGAATTGATGCAGGTGTAACGCCCGTTCTAAACTTCAAGGGTTATCTGGTGGGAAATGGAGTTACTGATGATAAATTCGATGGTAATGCTCTTGTTCCGTTTGCACATGGGATGGGACTGATATCAGATGATCTATATGAGGAGGTTAACACAGCGTGTGGAGGAAACTACCTTGATCCGGTGAGCGATCCTTGTGGAAGCAAGCTTGAAAAAGTTGACAAGAACATCGACGGTTTGAACATATATAACATTCTGGAACCATGCTATCACAGCACAGATTCGAGTAAGATTAGAAGAGTTACAAACAGTGGATTGCCATCTAGCTTTCGCCAATTAGGTGAGACTGAAAGGCCTCTTGCTGTGAGAAAAAGGATGTTTGGTCGTGCCTGGCCTCTTAGAGCTCCTGTGAGAGATGGTATTGTTCCAACTTGGCCGGAGCTGATGAATAGCGAAGAGGTTCCATGTACTGATGATGAGGTTGCTACTGAATGGTTGAACAACGAGACAGTTAGGAAGGCAATCCATGCAGAAACGGATCTTTCATGGGAACTATGCACAAACAGAATCAGATTTTATCATGATTCTGGAAGTATGATCAAGTACCACAGGAACCTCACCGCTAAGGGTTATCGGGCACTTATATTTAGCGGTGATCATGATATGTGTGTTCCTTTCACCGGGAGCGAAGCATGGACCAGATCACTTGGTTATAAGATTGTGGACGAATGGAGGCCATGGACTTCGAATGGACAAGTTGCTGGGTATACACAGGCTTACGAGAACAACCTCACCTTTCTAACCGTAAAGGGATCTGGACATACAGTTCCGGAGTACAAACCACGGGAGGCGTTAGATTTATTTAGCCGGTTTTTGGCCGGAAAACCACAATAA

Protein Analysis

495

Amino Acids

55.04

Weight (kDa)

5.53

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 36 - 490 3.3e-144 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1305
AarI CACCTGC 1 cut(s) 596
Acc36I ACCTGC 1 cut(s) 596
AccB1I GGYRCC 1 cut(s) 31
AccI GTMKAC 2 cut(s) 561, 1358
AccIII TCCGGA 1 cut(s) 1420
AciI CCGC 4 cut(s) 6, 218, 1212, 1242
AclWI GGATC 7 cut(s) 218, 231, 459, 764, 776, 1137, 1411
AcoI YGGCCR 2 cut(s) 1025, 1467
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 1 cut(s) 603
AdeI CACNNNGTG 1 cut(s) 750
AfaI GTAC 4 cut(s) 506, 1060, 1196, 1427
AfiI CCNNNNNNNGG 3 cut(s) 772, 1271, 1465
AgsI TTSAA 8 cut(s) 132, 301, 308, 500, 631, 803, 832, 1090
AjnI CCWGG 3 cut(s) 106, 253, 980
AjuI GAANNNNNNNTTGG 2 cut(s) 562, 594
AloI GAACNNNNNNTCC 2 cut(s) 1401, 1433
AluBI AGCT 9 cut(s) 57, 77, 267, 340, 360, 799, 918, 995, 1033
AluI AGCT 9 cut(s) 57, 77, 267, 340, 360, 799, 918, 995, 1033
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 3 cut(s) 425, 929, 1091
AlwI GGATC 7 cut(s) 218, 231, 459, 764, 776, 1137, 1411
Aor13HI TCCGGA 1 cut(s) 1420
AoxI GGCC 7 cut(s) 293, 312, 944, 983, 1025, 1325, 1467
ApoI RAATTY 1 cut(s) 668
AseI ATTAAT 1 cut(s) 38
Asp700I GAANNNNTTC 2 cut(s) 182, 1050
AspS9I GGNCC 3 cut(s) 251, 293, 1287
AsuC2I CCSGG 1 cut(s) 1273
AsuHPI GGTGA 6 cut(s) 787, 944, 1201, 1256, 1261, 1375
AsuII TTCGAA 1 cut(s) 1337
AvaII GGWCC 2 cut(s) 251, 1287
BaeGI GKGCMC 1 cut(s) 1231
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 997
Bbv12I GWGCWC 1 cut(s) 997
BbvCI CCTCAGC 1 cut(s) 336
BccI CCATC 5 cut(s) 266, 668, 700, 919, 1001
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 3 cut(s) 108, 255, 982
BclI TGATCA 2 cut(s) 1188, 1246
BcnI CCSGG 1 cut(s) 1273
BcoDI GTCTC 3 cut(s) 425, 929, 1091
BfaI CTAG 1 cut(s) 915
BfuAI ACCTGC 1 cut(s) 596
Bme1390I CCNGG 4 cut(s) 108, 255, 982, 1273
Bme18I GGWCC 2 cut(s) 251, 1287
BmgT120I GGNCC 3 cut(s) 251, 293, 1287
BmiI GGNNCC 6 cut(s) 33, 179, 225, 852, 1053, 1205
BmrFI CCNGG 4 cut(s) 108, 255, 982, 1273
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 592
BmuI ACTGGG 1 cut(s) 456
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 2 cut(s) 247, 576
Bpu10I CCTNAGC 2 cut(s) 336, 1215
Bpu14I TTCGAA 1 cut(s) 1337
BpuEI CTTGAG 2 cut(s) 73, 473
BpuMI CCSGG 1 cut(s) 1273
BsaAI YACGTR 1 cut(s) 565
BsaBI GATNNNNATC 1 cut(s) 910
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 2 cut(s) 1328, 1433
BsaWI WCCGGW 3 cut(s) 226, 772, 1420
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 3 cut(s) 772, 1271, 1465
Bse118I RCCGGY 1 cut(s) 1458
Bse1I ACTGG 1 cut(s) 451
Bse8I GATNNNNATC 1 cut(s) 910
BseAI TCCGGA 1 cut(s) 1420
BseBI CCWGG 3 cut(s) 108, 255, 982
BseDI CCNNGG 2 cut(s) 1328, 1433
BseGI GGATG 2 cut(s) 711, 972
BseJI GATNNNNATC 1 cut(s) 910
BseLI CCNNNNNNNGG 3 cut(s) 772, 1271, 1465
BseMII CTCAG 2 cut(s) 350, 501
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 3 cut(s) 69, 476, 749
BseSI GKGCMC 1 cut(s) 1231
BseYI CCCAGC 2 cut(s) 539, 1352
BshFI GGCC 7 cut(s) 295, 314, 946, 985, 1027, 1327, 1469
BshNI GGYRCC 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 997
BsiSI CCGG 8 cut(s) 227, 398, 773, 1028, 1272, 1421, 1459, 1470
BslFI GGGAC 1 cut(s) 723
BslI CCNNNNNNNGG 3 cut(s) 772, 1271, 1465
BsmAI GTCTC 3 cut(s) 425, 929, 1091
BsmFI GGGAC 1 cut(s) 723
BsnI GGCC 7 cut(s) 295, 314, 946, 985, 1027, 1327, 1469
Bso31I GGTCTC 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 1337
Bsp1286I GDGCHC 2 cut(s) 997, 1231
Bsp13I TCCGGA 1 cut(s) 1420
Bsp19I CCATGG 1 cut(s) 1328
BspACI CCGC 4 cut(s) 6, 218, 1212, 1242
BspANI GGCC 7 cut(s) 295, 314, 946, 985, 1027, 1327, 1469
BspCNI CTCAG 2 cut(s) 349, 502
BspEI TCCGGA 1 cut(s) 1420
BspHI TCATGA 2 cut(s) 1171, 1249
BspLI GGNNCC 6 cut(s) 33, 179, 225, 852, 1053, 1205
BspMI ACCTGC 1 cut(s) 596
BspPI GGATC 7 cut(s) 218, 231, 459, 764, 776, 1137, 1411
BspT104I TTCGAA 1 cut(s) 1337
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 425
BsrFI RCCGGY 1 cut(s) 1458
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 1458
BssECI CCNNGG 2 cut(s) 1328, 1433
BssNAI GTATAC 1 cut(s) 1359
BssT1I CCWWGG 1 cut(s) 1328
Bst1107I GTATAC 1 cut(s) 1359
Bst2UI CCWGG 3 cut(s) 108, 255, 982
Bst4CI ACNGT 7 cut(s) 157, 504, 827, 902, 1102, 1396, 1417
Bst6I CTCTTC 4 cut(s) 129, 303, 883, 1041
BstAPI GCANNNNNTGC 1 cut(s) 1118
BstBAI YACGTR 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 1337
BstC8I GCNNGC 2 cut(s) 216, 797
BstDEI CTNAG 4 cut(s) 336, 510, 989, 1215
BstDSI CCRYGG 2 cut(s) 1328, 1433
BstF5I GGATG 2 cut(s) 711, 972
BstMAI GTCTC 3 cut(s) 425, 929, 1091
BstMWI GCNNNNNNNGC 3 cut(s) 273, 864, 1118
BstNI CCWGG 3 cut(s) 108, 255, 982
BstSCI CCNGG 4 cut(s) 106, 253, 980, 1271
BstSLI GKGCMC 1 cut(s) 1231
BstX2I RGATCY 4 cut(s) 223, 451, 1129, 1403
BstXI CCANNNNNNTGG 3 cut(s) 107, 170, 360
BstYI RGATCY 4 cut(s) 223, 451, 1129, 1403
BstZ17I GTATAC 1 cut(s) 1359
BsuRI GGCC 7 cut(s) 295, 314, 946, 985, 1027, 1327, 1469
BtgI CCRYGG 2 cut(s) 1328, 1433
BtsCI GGATG 2 cut(s) 711, 972
BtsIMutI CAGTG 2 cut(s) 121, 907
BveI ACCTGC 1 cut(s) 596
Cac8I GCNNGC 2 cut(s) 216, 797
CciI TCATGA 2 cut(s) 1171, 1249
Cfr10I RCCGGY 1 cut(s) 1458
Cfr13I GGNCC 3 cut(s) 251, 293, 1287
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 4 cut(s) 505, 1059, 1195, 1426
CviQI GTAC 4 cut(s) 505, 1059, 1195, 1426
DdeI CTNAG 4 cut(s) 336, 510, 989, 1215
DraIII CACNNNGTG 1 cut(s) 750
EaeI YGGCCR 2 cut(s) 1025, 1467
Eam1104I CTCTTC 4 cut(s) 129, 303, 883, 1041
EarI CTCTTC 4 cut(s) 129, 303, 883, 1041
Ecl136II GAGCTC 1 cut(s) 995
Eco130I CCWWGG 1 cut(s) 1328
Eco147I AGGCCT 1 cut(s) 946
Eco24I GRGCYC 1 cut(s) 997
Eco31I GGTCTC 1 cut(s) 425
Eco32I GATATC 1 cut(s) 717
Eco47I GGWCC 2 cut(s) 251, 1287
Eco53kI GAGCTC 1 cut(s) 995
Eco57I CTGAAG 1 cut(s) 603
EcoICRI GAGCTC 1 cut(s) 995
EcoRII CCWGG 3 cut(s) 106, 253, 980
EcoRV GATATC 1 cut(s) 717
EcoT14I CCWWGG 1 cut(s) 1328
EcoT38I GRGCYC 1 cut(s) 997
ErhI CCWWGG 1 cut(s) 1328
FaqI GGGAC 1 cut(s) 723
FauNDI CATATG 1 cut(s) 550
FbaI TGATCA 2 cut(s) 1188, 1246
FblI GTMKAC 2 cut(s) 561, 1358
FokI GGATG 2 cut(s) 718, 979
FriOI GRGCYC 1 cut(s) 997
FspBI CTAG 1 cut(s) 915
GsaI CCCAGC 2 cut(s) 543, 1356
GsuI CTGGAG 2 cut(s) 247, 576
HaeIII GGCC 7 cut(s) 295, 314, 946, 985, 1027, 1327, 1469
HapII CCGG 8 cut(s) 227, 398, 773, 1028, 1272, 1421, 1459, 1470
HincII GTYRAC 3 cut(s) 160, 742, 811
HindII GTYRAC 3 cut(s) 160, 742, 811
HindIII AAGCTT 1 cut(s) 797
HpaI GTTAAC 2 cut(s) 160, 742
HpaII CCGG 8 cut(s) 227, 398, 773, 1028, 1272, 1421, 1459, 1470
HphI GGTGA 6 cut(s) 787, 944, 1201, 1256, 1261, 1375
Hpy166II GTNNAC 7 cut(s) 160, 251, 562, 742, 811, 1315, 1359
Hpy188I TCNGA 5 cut(s) 205, 469, 583, 721, 1163
Hpy188III TCNNGA 8 cut(s) 52, 389, 848, 1172, 1179, 1250, 1407, 1421
Hpy8I GTNNAC 7 cut(s) 160, 251, 562, 742, 811, 1315, 1359
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 3 cut(s) 138, 200, 1102
HpyCH4III ACNGT 7 cut(s) 157, 504, 827, 902, 1102, 1396, 1417
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 4 cut(s) 605, 698, 1121, 1149
HpyF10VI GCNNNNNNNGC 3 cut(s) 273, 864, 1118
HpyF3I CTNAG 4 cut(s) 336, 510, 989, 1215
HpySE526I ACGT 1 cut(s) 564
Kpn2I TCCGGA 1 cut(s) 1420
Ksp22I TGATCA 2 cut(s) 1188, 1246
KspAI GTTAAC 2 cut(s) 160, 742
LmnI GCTCC 6 cut(s) 82, 266, 345, 1000, 1030, 1275
LweI GCATC 1 cut(s) 592
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 1 cut(s) 915
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 4 cut(s) 151, 610, 655, 892
MboII GAAGA 6 cut(s) 40, 116, 320, 570, 900, 1058
MflI RGATCY 4 cut(s) 223, 451, 1129, 1403
MhlI GDGCHC 2 cut(s) 997, 1231
MluCI AATT 4 cut(s) 301, 597, 668, 926
MlyI GAGTC 3 cut(s) 175, 554, 567
MmeI TCCRAC 4 cut(s) 388, 399, 546, 1043
MroI TCCGGA 1 cut(s) 1420
MroXI GAANNNNTTC 2 cut(s) 182, 1050
MseI TTAA 4 cut(s) 38, 159, 348, 741
MspA1I CMGCKG 1 cut(s) 360
MspI CCGG 8 cut(s) 227, 398, 773, 1028, 1272, 1421, 1459, 1470
MspR9I CCNGG 4 cut(s) 108, 255, 982, 1273
MvaI CCWGG 3 cut(s) 108, 255, 982
MwoI GCNNNNNNNGC 3 cut(s) 273, 864, 1118
NciI CCSGG 1 cut(s) 1273
NcoI CCATGG 1 cut(s) 1328
NdeI CATATG 1 cut(s) 550
NlaIV GGNNCC 6 cut(s) 33, 179, 225, 852, 1053, 1205
NmuCI GTSAC 1 cut(s) 151
NspV TTCGAA 1 cut(s) 1337
PagI TCATGA 2 cut(s) 1171, 1249
PaqCI CACCTGC 1 cut(s) 596
PceI AGGCCT 1 cut(s) 946
PdmI GAANNNNTTC 2 cut(s) 182, 1050
PfeI GAWTC 8 cut(s) 103, 111, 200, 392, 470, 872, 1158, 1175
PfoI TCCNGGA 1 cut(s) 106
PleI GAGTC 3 cut(s) 174, 553, 566
PpsI GAGTC 3 cut(s) 174, 553, 566
Ppu21I YACGTR 1 cut(s) 565
PshBI ATTAAT 1 cut(s) 38
PsiI TTATAA 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 997
Psp6I CCWGG 3 cut(s) 106, 253, 980
PspFI CCCAGC 2 cut(s) 539, 1352
PspGI CCWGG 3 cut(s) 106, 253, 980
PspN4I GGNNCC 6 cut(s) 33, 179, 225, 852, 1053, 1205
PspPI GGNCC 3 cut(s) 251, 293, 1287
PsrI GAACNNNNNNTAC 6 cut(s) 170, 202, 497, 529, 603, 635
PsuI RGATCY 4 cut(s) 223, 451, 1129, 1403
PvuII CAGCTG 1 cut(s) 360
RsaI GTAC 4 cut(s) 506, 1060, 1196, 1427
RsaNI GTAC 4 cut(s) 505, 1059, 1195, 1426
SacI GAGCTC 1 cut(s) 997
SaqAI TTAA 4 cut(s) 38, 159, 348, 741
Sau96I GGNCC 3 cut(s) 251, 293, 1287
SchI GAGTC 3 cut(s) 175, 554, 567
ScrFI CCNGG 4 cut(s) 108, 255, 982, 1273
SduI GDGCHC 2 cut(s) 997, 1231
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 1 cut(s) 592
SfuI TTCGAA 1 cut(s) 1337
SinI GGWCC 2 cut(s) 251, 1287
SmlI CTYRAG 2 cut(s) 52, 488
SmoI CTYRAG 2 cut(s) 52, 488
Sse9I AATT 4 cut(s) 301, 597, 668, 926
SseBI AGGCCT 1 cut(s) 946
SsiI CCGC 4 cut(s) 6, 218, 1212, 1242
SspMI CTAG 1 cut(s) 915
SstI GAGCTC 1 cut(s) 997
StuI AGGCCT 1 cut(s) 946
StyD4I CCNGG 4 cut(s) 106, 253, 980, 1271
StyI CCWWGG 1 cut(s) 1328
TaaI ACNGT 7 cut(s) 157, 504, 827, 902, 1102, 1396, 1417
TaiI ACGT 1 cut(s) 567
TaqI TCGA 5 cut(s) 198, 672, 822, 875, 1337
TaqII GACCGA 1 cut(s) 449
TasI AATT 4 cut(s) 301, 597, 668, 926
TatI WGTACW 2 cut(s) 1058, 1425
TfiI GAWTC 8 cut(s) 103, 111, 200, 392, 470, 872, 1158, 1175
Tru1I TTAA 4 cut(s) 38, 159, 348, 741
Tru9I TTAA 4 cut(s) 38, 159, 348, 741
TscAI CASTG 2 cut(s) 121, 907
TseFI GTSAC 1 cut(s) 151
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 2 cut(s) 1052, 1125
TspGWI ACGGA 2 cut(s) 681, 1142
TspRI CASTG 2 cut(s) 121, 907
VpaK11BI GGWCC 2 cut(s) 251, 1287
VspI ATTAAT 1 cut(s) 38
XapI RAATTY 1 cut(s) 668
XmiI GTMKAC 2 cut(s) 561, 1358
XmnI GAANNNNTTC 2 cut(s) 182, 1050
XspI CTAG 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.