FvH4_2g17690

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002596
Physical Location & Seq
Reverse (-)
15197929 .. 15201823
3895 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g17690.t1

Sequence Viewer

Length: 1491 bp
ATGGCCAAGACTAGTATTACTGTATTATATTCACTCTTATCAGTCACGTTCTTGAGCTTGGTGCCAACCCAATCAGCTCCTGAGGCAGCTCTCATAACCCAGATTCCTGGATTCAACGGCACTCTTCCTTCAAAGCACCGTTCTGGGTATGTGACAATTGATGAGAGTCATGGGAGGAACTTGTTTTACTATTTTGTGGAATCGGAGAGGAAGCCGTCGGAGGATCCTGTGGTGCTGTGGCTGAATGGTGGACCTGGTTGCTCCAGTTTTGATGGCTTTGTCTATGAGCATGGGCCATTCAATTTTGAAGCAGCAAAGACCAAGGGAGGTCTGCCCAAATTGCACCTTAATCCATACAGCTGGTCAAAGGTTTCCAACATTTTATATTTGGATTCTCCTGCTGGTGTTGGGCTTTCTTACTCCAAAAACAAATCTGACTATATTACTGGTGATCTAAAGACTGCATCTGATAGCCACACATTTCTCCTAAAGTGGTTTGAACTTTACCCAGAGTTCCTCTCCAACCCGTTTTTCATTTCGGGAGAGTCTTATGCTGGTGTATATGTGCCAACACTTGCTTCAGCAGTTGTGAAAGGAATTGATGCTGGTACAAAGCCCAAAATCAACTTCAAGGGTTATCTTGTGGGAAATGGAGTCACAGATGAGGAATTCGATGGGAATGCCATTGTTCCATTTGCTCATGGGATGGGACTTATATCAGATGATCTATTTGAGGGGGTAATCAATGAGTGCAATGGAAACTACTATGAGCCAAATAGTGAAACTTGTGACAACAAGCTTGAAAAAGTTGACAACGCCATAGAAGGTTTGAACATCTATGACATTCTAGAACCATGCTATCACAGCACAGATTCTATGAAAATTTCAACCGTAACTAGTAACATACCATCTAGCTTTCGAAGGTTGGGTGAAACTGAAAGGCCTCTTGCTGTGAGAAAAAGGATGTTTGGTCGAGCCTGGCCTCTTAGAGCTCCTGTAAGAGCTGGACTGGTTCCAACTTGGCCACAGCTTATGAATAGTGAGGAAGTTCCATGCACTGATGATGAGGTTGCAACTGCATGGTTGAACAATGCAGCAGTTAGGAAGGCAATCCATGCAGAAGATGCAAGCGTGATTCCCAGTTGGGAATTGTGTACAGACAACATTCGTTTTTACCATGATGCTGGGAGTATGATCCCATACCACAAGAATCTGACTTCGAAGGGATACCGTGCACTTATATATAGTGGTGATCATGATTTGTGTGTTCCATTCACCGGGAGTGAAGCATGGACTAGATCACTTGGTTACAAAATTGTGGATGAATGGAGGCCATGGACTTCCAAGGGACAAATTGCTGGGTATACACAAGGGTACGAAAAAAACCTCACATTTTCAACCGTAAAGGGCTCCGGTCATACGGTTCCAGAATACAAACCTCAAGAGGCATTAGATTTATATAGCCGATTCTTGGCTGGAAAACCACTTTAG

Protein Analysis

497

Amino Acids

54.95

Weight (kDa)

5.61

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 36 - 492 2.5e-143 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 1364
AclWI GGATC 3 cut(s) 218, 231, 1189
AcoI YGGCCR 2 cut(s) 3, 1022
AcsI RAATTY 2 cut(s) 668, 882
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 3 cut(s) 610, 1156, 1376
AfiI CCNNNNNNNGG 2 cut(s) 1277, 1471
AhlI ACTAGT 2 cut(s) 11, 896
AjnI CCWGG 3 cut(s) 106, 253, 977
AjuI GAANNNNNNNTTGG 6 cut(s) 515, 547, 562, 594, 611, 643
AluBI AGCT 9 cut(s) 57, 77, 89, 360, 799, 915, 992, 1004, 1030
AluI AGCT 9 cut(s) 57, 77, 89, 360, 799, 915, 992, 1004, 1030
Alw21I GWGCWC 2 cut(s) 994, 1237
Alw44I GTGCAC 1 cut(s) 1233
AlwI GGATC 3 cut(s) 218, 231, 1189
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 6 cut(s) 3, 293, 941, 980, 1022, 1331
ApaLI GTGCAC 1 cut(s) 1233
ApeKI GCWGC 3 cut(s) 86, 311, 1094
ApoI RAATTY 2 cut(s) 668, 882
AspS9I GGNCC 2 cut(s) 251, 293
AsuC2I CCSGG 1 cut(s) 1279
AsuHPI GGTGA 4 cut(s) 461, 941, 1262, 1267
AsuII TTCGAA 2 cut(s) 919, 1220
AvaII GGWCC 1 cut(s) 251
AxyI CCTNAGG 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 1237
BalI TGGCCA 2 cut(s) 5, 1024
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 2 cut(s) 994, 1412
Bbv12I GWGCWC 2 cut(s) 994, 1237
BbvI GCAGC 3 cut(s) 98, 323, 1106
BccI CCATC 4 cut(s) 266, 668, 700, 916
BceAI ACGGC 2 cut(s) 133, 199
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 3 cut(s) 108, 255, 979
BciVI GTATCC 1 cut(s) 1220
BclI TGATCA 1 cut(s) 1252
BcnI CCSGG 1 cut(s) 1279
BcuI ACTAGT 2 cut(s) 11, 896
BfaI CTAG 5 cut(s) 12, 848, 897, 912, 1296
BfuI GTATCC 1 cut(s) 1220
BisI GCNGC 3 cut(s) 87, 312, 1095
BlsI GCNGC 3 cut(s) 88, 313, 1096
Bme1390I CCNGG 4 cut(s) 108, 255, 979, 1279
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 251, 293
BmiI GGNNCC 5 cut(s) 63, 225, 1014, 1411, 1425
BmrFI CCNGG 4 cut(s) 108, 255, 979, 1279
BmrI ACTGGG 1 cut(s) 1134
BmsI GCATC 4 cut(s) 473, 592, 1114, 1171
BmuI ACTGGG 1 cut(s) 1134
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 1 cut(s) 247
Bpu14I TTCGAA 2 cut(s) 919, 1220
BpuEI CTTGAG 2 cut(s) 73, 1425
BpuMI CCSGG 1 cut(s) 1279
BsaJI CCNNGG 3 cut(s) 321, 1334, 1344
BsaWI WCCGGW 1 cut(s) 1412
Bsc4I CCNNNNNNNGG 2 cut(s) 1277, 1471
Bse1I ACTGG 4 cut(s) 264, 451, 1014, 1140
Bse21I CCTNAGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 760
BseBI CCWGG 3 cut(s) 108, 255, 979
BseDI CCNNGG 3 cut(s) 321, 1334, 1344
BseGI GGATG 3 cut(s) 711, 969, 1327
BseLI CCNNNNNNNGG 2 cut(s) 1277, 1471
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 4 cut(s) 264, 451, 1014, 1140
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 3 cut(s) 98, 323, 1106
BseYI CCCAGC 2 cut(s) 1184, 1358
BshFI GGCC 6 cut(s) 5, 295, 943, 982, 1024, 1333
BshNI GGYRCC 1 cut(s) 61
BsiHKAI GWGCWC 2 cut(s) 994, 1237
BsiSI CCGG 2 cut(s) 1278, 1413
BslFI GGGAC 2 cut(s) 723, 1362
BslI CCNNNNNNNGG 2 cut(s) 1277, 1471
BsmFI GGGAC 2 cut(s) 723, 1362
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 6 cut(s) 5, 295, 943, 982, 1024, 1333
Bsp119I TTCGAA 2 cut(s) 919, 1220
Bsp1286I GDGCHC 3 cut(s) 994, 1237, 1412
Bsp1407I TGTACA 1 cut(s) 1154
Bsp143I GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
Bsp19I CCATGG 1 cut(s) 1334
BspANI GGCC 6 cut(s) 5, 295, 943, 982, 1024, 1333
BspCNI CTCAG 1 cut(s) 73
BspHI TCATGA 1 cut(s) 1255
BspLI GGNNCC 5 cut(s) 63, 225, 1014, 1411, 1425
BspPI GGATC 3 cut(s) 218, 231, 1189
BspT104I TTCGAA 2 cut(s) 919, 1220
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 760
BsrGI TGTACA 1 cut(s) 1154
BsrI ACTGG 4 cut(s) 264, 451, 1014, 1140
BssECI CCNNGG 3 cut(s) 321, 1334, 1344
BssMI GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
BssNAI GTATAC 1 cut(s) 1365
BssT1I CCWWGG 3 cut(s) 321, 1334, 1344
Bst1107I GTATAC 1 cut(s) 1365
Bst2UI CCWGG 3 cut(s) 108, 255, 979
Bst4CI ACNGT 6 cut(s) 22, 140, 892, 1232, 1402, 1423
Bst6I CTCTTC 1 cut(s) 129
BstAPI GCANNNNNTGC 2 cut(s) 1115, 1124
BstAUI TGTACA 1 cut(s) 1154
BstBI TTCGAA 2 cut(s) 919, 1220
BstC8I GCNNGC 1 cut(s) 1129
BstDEI CTNAG 2 cut(s) 81, 986
BstDSI CCRYGG 1 cut(s) 1334
BstF5I GGATG 3 cut(s) 711, 969, 1327
BstKTI GATC 6 cut(s) 226, 454, 727, 1197, 1255, 1301
BstMBI GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
BstMWI GCNNNNNNNGC 5 cut(s) 83, 340, 864, 1115, 1124
BstNI CCWGG 3 cut(s) 108, 255, 979
BstSCI CCNGG 4 cut(s) 106, 253, 977, 1277
BstSLI GKGCMC 1 cut(s) 1237
BstV1I GCAGC 3 cut(s) 98, 323, 1106
BstX2I RGATCY 1 cut(s) 223
BstXI CCANNNNNNTGG 3 cut(s) 107, 360, 1184
BstYI RGATCY 1 cut(s) 223
BstZ17I GTATAC 1 cut(s) 1365
Bsu36I CCTNAGG 1 cut(s) 81
BsuI GTATCC 1 cut(s) 1220
BsuRI GGCC 6 cut(s) 5, 295, 943, 982, 1024, 1333
BtgI CCRYGG 1 cut(s) 1334
BtsCI GGATG 3 cut(s) 711, 969, 1327
BtsIMutI CAGTG 1 cut(s) 1056
Cac8I GCNNGC 1 cut(s) 1129
CaiI CAGNNNCTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 1255
Cfr13I GGNCC 2 cut(s) 251, 293
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 3 cut(s) 609, 1155, 1375
CviQI GTAC 3 cut(s) 609, 1155, 1375
DdeI CTNAG 2 cut(s) 81, 986
DpnI GATC 6 cut(s) 225, 453, 726, 1196, 1254, 1300
DpnII GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
EaeI YGGCCR 2 cut(s) 3, 1022
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 992
Eco130I CCWWGG 3 cut(s) 321, 1334, 1344
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 994, 1412
Eco47I GGWCC 1 cut(s) 251
Eco53kI GAGCTC 1 cut(s) 992
Eco57I CTGAAG 1 cut(s) 564
Eco81I CCTNAGG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 668
EcoRII CCWGG 3 cut(s) 106, 253, 977
EcoT14I CCWWGG 3 cut(s) 321, 1334, 1344
EcoT38I GRGCYC 2 cut(s) 994, 1412
ErhI CCWWGG 3 cut(s) 321, 1334, 1344
FaqI GGGAC 2 cut(s) 723, 1362
FbaI TGATCA 1 cut(s) 1252
FblI GTMKAC 1 cut(s) 1364
Fnu4HI GCNGC 3 cut(s) 87, 312, 1095
FokI GGATG 3 cut(s) 718, 976, 1334
FriOI GRGCYC 2 cut(s) 994, 1412
Fsp4HI GCNGC 3 cut(s) 87, 312, 1095
FspBI CTAG 5 cut(s) 12, 848, 897, 912, 1296
GluI GCNGC 3 cut(s) 87, 312, 1095
GsaI CCCAGC 2 cut(s) 1188, 1362
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 6 cut(s) 5, 295, 943, 982, 1024, 1333
HapII CCGG 2 cut(s) 1278, 1413
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 797
HpaII CCGG 2 cut(s) 1278, 1413
HphI GGTGA 4 cut(s) 461, 941, 1262, 1267
Hpy166II GTNNAC 5 cut(s) 251, 811, 1155, 1235, 1365
Hpy188I TCNGA 6 cut(s) 205, 220, 436, 469, 721, 1215
Hpy188III TCNNGA 7 cut(s) 52, 80, 540, 848, 1256, 1427, 1442
Hpy8I GTNNAC 5 cut(s) 251, 811, 1155, 1235, 1365
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 5 cut(s) 138, 818, 915, 1099, 1216
HpyCH4III ACNGT 6 cut(s) 22, 140, 892, 1232, 1402, 1423
HpyCH4IV ACGT 1 cut(s) 47
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 340, 864, 1115, 1124
HpyF3I CTNAG 2 cut(s) 81, 986
HpySE526I ACGT 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 1252
Kzo9I GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
LmnI GCTCC 4 cut(s) 82, 266, 997, 1415
Lsp1109I GCAGC 3 cut(s) 98, 323, 1106
LweI GCATC 4 cut(s) 473, 592, 1114, 1171
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 5 cut(s) 12, 848, 897, 912, 1296
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 7 cut(s) 43, 151, 655, 788, 892, 899, 1307
MalI GATC 6 cut(s) 225, 453, 726, 1196, 1254, 1300
MboI GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
MboII GAAGA 2 cut(s) 116, 1133
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 3 cut(s) 994, 1237, 1412
MlsI TGGCCA 2 cut(s) 5, 1024
MluCI AATT 9 cut(s) 156, 301, 338, 597, 668, 882, 1148, 1314, 1353
MluNI TGGCCA 2 cut(s) 5, 1024
MlyI GAGTC 3 cut(s) 175, 554, 663
MmeI TCCRAC 4 cut(s) 198, 399, 546, 1040
Mox20I TGGCCA 2 cut(s) 5, 1024
MscI TGGCCA 2 cut(s) 5, 1024
MseI TTAA 1 cut(s) 348
Msp20I TGGCCA 2 cut(s) 5, 1024
MspA1I CMGCKG 1 cut(s) 360
MspI CCGG 2 cut(s) 1278, 1413
MspR9I CCNGG 4 cut(s) 108, 255, 979, 1279
MunI CAATTG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 3 cut(s) 108, 255, 979
MwoI GCNNNNNNNGC 5 cut(s) 83, 340, 864, 1115, 1124
NciI CCSGG 1 cut(s) 1279
NcoI CCATGG 1 cut(s) 1334
NdeII GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
NlaIV GGNNCC 5 cut(s) 63, 225, 1014, 1411, 1425
NmuCI GTSAC 4 cut(s) 43, 151, 655, 788
NspV TTCGAA 2 cut(s) 919, 1220
PagI TCATGA 1 cut(s) 1255
PceI AGGCCT 1 cut(s) 943
PctI GAATGC 1 cut(s) 685
PfeI GAWTC 8 cut(s) 103, 111, 200, 392, 872, 1135, 1210, 1467
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 3 cut(s) 88, 313, 1096
PleI GAGTC 3 cut(s) 174, 553, 662
PpsI GAGTC 3 cut(s) 174, 553, 662
Psp124BI GAGCTC 1 cut(s) 994
Psp6I CCWGG 3 cut(s) 106, 253, 977
PspFI CCCAGC 2 cut(s) 1184, 1358
PspGI CCWGG 3 cut(s) 106, 253, 977
PspN4I GGNNCC 5 cut(s) 63, 225, 1014, 1411, 1425
PspPI GGNCC 2 cut(s) 251, 293
PsrI GAACNNNNNNTAC 2 cut(s) 170, 202
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 223
PvuII CAGCTG 1 cut(s) 360
RsaI GTAC 3 cut(s) 610, 1156, 1376
RsaNI GTAC 3 cut(s) 609, 1155, 1375
SacI GAGCTC 1 cut(s) 994
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 87, 312, 1095
Sau3AI GATC 6 cut(s) 223, 451, 724, 1194, 1252, 1298
Sau96I GGNCC 2 cut(s) 251, 293
SchI GAGTC 3 cut(s) 175, 554, 663
ScrFI CCNGG 4 cut(s) 108, 255, 979, 1279
SduI GDGCHC 3 cut(s) 994, 1237, 1412
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 4 cut(s) 473, 592, 1114, 1171
SfuI TTCGAA 2 cut(s) 919, 1220
SinI GGWCC 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 52, 1440
SmoI CTYRAG 2 cut(s) 52, 1440
SpeI ACTAGT 2 cut(s) 11, 896
Sse9I AATT 9 cut(s) 156, 301, 338, 597, 668, 882, 1148, 1314, 1353
SseBI AGGCCT 1 cut(s) 943
SspMI CTAG 5 cut(s) 12, 848, 897, 912, 1296
SstI GAGCTC 1 cut(s) 994
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 4 cut(s) 106, 253, 977, 1277
StyI CCWWGG 3 cut(s) 321, 1334, 1344
TaaI ACNGT 6 cut(s) 22, 140, 892, 1232, 1402, 1423
TaiI ACGT 1 cut(s) 50
TaqI TCGA 4 cut(s) 672, 919, 973, 1220
TasI AATT 9 cut(s) 156, 301, 338, 597, 668, 882, 1148, 1314, 1353
TatI WGTACW 1 cut(s) 1154
TfiI GAWTC 8 cut(s) 103, 111, 200, 392, 872, 1135, 1210, 1467
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 1063
TseFI GTSAC 4 cut(s) 43, 151, 655, 788
TseI GCWGC 3 cut(s) 86, 311, 1094
Tsp45I GTSAC 4 cut(s) 43, 151, 655, 788
TspDTI ATGAA 4 cut(s) 523, 893, 1049, 1338
TspRI CASTG 1 cut(s) 1063
VneI GTGCAC 1 cut(s) 1233
VpaK11BI GGWCC 1 cut(s) 251
XapI RAATTY 2 cut(s) 668, 882
XbaI TCTAGA 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 1364
XspI CTAG 5 cut(s) 12, 848, 897, 912, 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.