Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002596

Physical Location & Seq

Genomic Coordinates Reverse (-)

23811362 .. 23818065

Gene Structure 6704 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1151200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGGCTGGGAGAATATTATATTTATTTTTTGTGCCATTAATTTACACTTTCTTGAGCATTTTGCCAACCCAATCAGCTCCTCAATCTGCACTCGTATCCCAGATTCCTGGATTCAGTGGCACTCTTCCTTCAAAACATTATGCTGGGTATGTGACAGTTGACCAGAGTCATGGGAGGAACCTGTTCTACTATTTCGTCGAATCAGAAGGGAAGCCGGCGGAGGATCCGGTGGTTCTGTGGCTCAATGGTGGACCTGGATGCTCCAGCTTTGATGGCTTTGTTTATGAGCATGGGCCATTCAATTTTGAAAAGGCCAAGACCAAGGGAAGTCTGACCCAACTCCACCTTAATCCATATAGCTGGTCGAAGGTTTCCAACATTATATATCTGGACTCTCCTGCTGGTGTTGGATTTTCTTACTCAAAAAATGAGACTGACTATGAAACTGGGGATCTAAAGACTGCCTCTGATTCCCACGCATTTCTCCTCAAGTGGTTTGAACTTTATCCTGAGTTCCTCTCCAACCCATTTTTTATTGCTGGGGAGTCATATGCCGGAGTCTACGTGCCGACGCTTTCTTCTGAAGTAGTGAAAGGAATTGATGCTGGTGTAACGCCTGTTCTCAACTTCAAGGGTTATCTGGTGGGAAATGGCGTTACGGATGATAAATTCGATGGTAACGCTCTTGTTCCGTTTGCACACGGGATGGGAATGATATCAGATGATCTATACGAGGTTAACACGGCGTGCGGAGGAAACTACATTTATCCGGTGAATGATAATTGTGAGAGCAAGCTTAAAAAAGTTGACAAGAACATAGAAGATTTGAACATATATGACATTCTGGAACCATGCTATCACAGCACAGATTCGAGTAAGAACAGAAAAGTTACAAAGAGCGGATTGCCATCTAGCTTTCGACGGTTGGGTGAGACTGAAAGGCCACTTGCTGTCAGAAAAAGAATGTTTGGTCGTGCCTGGCCTCTTAGAGCTCCTGTGAGGGATGGTATTGTTCCAACTTGGCCGGAGCTGATGGATAGTGAAAGTGTTCCGTGTATTAATGATGAGGTTGCTACTGAATGGTTGAACAACGAAACTGTTAGGAAGGCAATCCATGCAGAAACGGATTTTTCATGGGAATTATGCACAAGCAGAATTGAATTTTATCATGATTCTGGAAGTATGATCAAGTACCACAAGAACCTCACTGCAAAGGGTTATCGGGCACTTATATATAGCGGTGATCATGATATGTGTGTTCCTTTCACTGGGAGTGAAGCATGGACTAGATCACTTGGTTATAAGATTGTGGATGAATGGAGGCCATGGACTTCGAACGGACAAGTTGCTGGGTACACACAGGGTTACGAGAATAACCTCACCTTTCTAACTGTAAAGGGGTCTGGACATACAGTTCCAGAGTACAAACCACGAGAGGCGTTTGATTTGTTTAGCCGGTTTTTGGCTGGAAAACCACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005777 GO:0008150 GO:0008152 GO:0016740 GO:0016746 GO:0016747 GO:0019748 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

494

Amino Acids

55.18

Weight (kDa)

5.61

Isoelectric Point (pI)

38.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	36 - 489	5.2e-144	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002596)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25420 AT4G12910 AT4G12910
fragaria_vesca	FvH4_2g17690 FvH4_6g00420 FvH4_6g00420 FvH4_6g00420
malus_domestica	MD00G1170000.v1.1 MD04G1245700.v1.1 MD05G1158200.v1.1 MD10G1151200.v1.1 MD12G1264200.v1.1 MD13G1061200.v1.1
prunus_persica	Prupe.6G362200_v2.0.a1 Prupe.8G189600_v2.0.a1
pyrus_communis	pycom04g21660 pycom05g15020 pycom10g13030 pycom12g23930
rosa_chinensis	RchiOBHm_Chr3g0447751 RchiOBHm_Chr6g0282671
rosa_laevigata	RLG00000002738
rosa_multiflora	Rmu_co8425633.1_g000001 Rmu_ssc0000391.1_g000016
rosa_roxburghii	Rroxscaffold_3G00245270 Rroxscaffold_6G00425950
rosa_rugosa	Rorug02G0605300 Rorug06G0148500 Rorug07G0145800
rosa_samantha	Rh3AG005000 Rh3BG004600 Rh3CG005000 Rh3DG004900 Rh6AG261900 Rh6BG265000 Rh6CG264100 Rh6DG256300 Rh7BG273200
rosa_wichuraiana	Rw3G000370 Rw6G022640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1302
AccBSI	CCGCTC	1 cut(s)	900
AccI	GTMKAC	1 cut(s)	561
AciI	CCGC	4 cut(s)	218, 750, 900, 1239
AclWI	GGATC	3 cut(s)	218, 231, 459
AcoI	YGGCCR	1 cut(s)	1022
AcsI	RAATTY	2 cut(s)	668, 1160
AcuI	CTGAAG	1 cut(s)	603
AdeI	CACNNNGTG	1 cut(s)	747
AfaI	GTAC	3 cut(s)	1193, 1355, 1424
AfiI	CCNNNNNNNGG	2 cut(s)	1268, 1462
AgsI	TTSAA	8 cut(s)	132, 301, 308, 500, 631, 829, 1087, 1160
AjnI	CCWGG	3 cut(s)	106, 253, 977
AloI	GAACNNNNNNTCC	2 cut(s)	1398, 1430
AluBI	AGCT	7 cut(s)	77, 267, 360, 796, 915, 992, 1030
AluI	AGCT	7 cut(s)	77, 267, 360, 796, 915, 992, 1030
Alw21I	GWGCWC	1 cut(s)	994
Alw26I	GTCTC	2 cut(s)	425, 926
AlwI	GGATC	3 cut(s)	218, 231, 459
AoxI	GGCC	6 cut(s)	293, 312, 941, 980, 1022, 1322
ApoI	RAATTY	2 cut(s)	668, 1160
AseI	ATTAAT	2 cut(s)	38, 1059
Asp700I	GAANNNNTTC	2 cut(s)	182, 1047
AspS9I	GGNCC	2 cut(s)	251, 293
AsuHPI	GGTGA	4 cut(s)	784, 941, 1253, 1372
AsuII	TTCGAA	1 cut(s)	1334
AvaII	GGWCC	1 cut(s)	251
BaeGI	GKGCMC	1 cut(s)	1228
BamHI	GGATCC	1 cut(s)	223
BanII	GRGCYC	1 cut(s)	994
BauI	CACGAG	1 cut(s)	1431
Bbv12I	GWGCWC	1 cut(s)	994
BccI	CCATC	6 cut(s)	266, 668, 700, 916, 998, 1027
BceAI	ACGGC	1 cut(s)	759
BciT130I	CCWGG	3 cut(s)	108, 255, 979
BciVI	GTATCC	1 cut(s)	106
BclI	TGATCA	2 cut(s)	1185, 1243
BcoDI	GTCTC	2 cut(s)	425, 926
BfaI	CTAG	2 cut(s)	912, 1287
BfuI	GTATCC	1 cut(s)	106
Bme1390I	CCNGG	3 cut(s)	108, 255, 979
Bme18I	GGWCC	1 cut(s)	251
BmgT120I	GGNCC	2 cut(s)	251, 293
BmiI	GGNNCC	3 cut(s)	179, 225, 849
BmrFI	CCNGG	3 cut(s)	108, 255, 979
BmrI	ACTGGG	2 cut(s)	456, 1278
BmsI	GCATC	2 cut(s)	248, 592
BmuI	ACTGGG	2 cut(s)	456, 1278
BoxI	GACNNNNGTC	1 cut(s)	165
BplI	GAGNNNNNCTC	2 cut(s)	503, 535
BpmI	CTGGAG	1 cut(s)	247
Bpu14I	TTCGAA	1 cut(s)	1334
BpuEI	CTTGAG	2 cut(s)	73, 473
BsaAI	YACGTR	1 cut(s)	565
BsaBI	GATNNNNATC	1 cut(s)	907
BsaJI	CCNNGG	2 cut(s)	321, 1325
BsaWI	WCCGGW	2 cut(s)	226, 769
BsaXI	ACNNNNNCTCC	2 cut(s)	549, 579
Bsc4I	CCNNNNNNNGG	2 cut(s)	1268, 1462
Bse118I	RCCGGY	2 cut(s)	214, 1455
Bse1I	ACTGG	2 cut(s)	451, 1273
Bse8I	GATNNNNATC	1 cut(s)	907
BseBI	CCWGG	3 cut(s)	108, 255, 979
BseDI	CCNNGG	2 cut(s)	321, 1325
BseGI	GGATG	5 cut(s)	263, 667, 711, 1009, 1318
BseJI	GATNNNNATC	1 cut(s)	907
BseLI	CCNNNNNNNGG	2 cut(s)	1268, 1462
BseMII	CTCAG	1 cut(s)	501
BseNI	ACTGG	2 cut(s)	451, 1273
BseRI	GAGGAG	2 cut(s)	69, 476
BseSI	GKGCMC	1 cut(s)	1228
BseYI	CCCAGC	4 cut(s)	5, 143, 539, 1349
BsgI	GTGCAG	1 cut(s)	72
BshFI	GGCC	6 cut(s)	295, 314, 943, 982, 1024, 1324
BsiHKAI	GWGCWC	1 cut(s)	994
BsiSI	CCGG	6 cut(s)	215, 227, 555, 770, 1025, 1456
BslI	CCNNNNNNNGG	2 cut(s)	1268, 1462
BsmAI	GTCTC	2 cut(s)	425, 926
BsnI	GGCC	6 cut(s)	295, 314, 943, 982, 1024, 1324
Bsp119I	TTCGAA	1 cut(s)	1334
Bsp1286I	GDGCHC	2 cut(s)	994, 1228
Bsp143I	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
Bsp19I	CCATGG	1 cut(s)	1325
BspACI	CCGC	4 cut(s)	218, 750, 900, 1239
BspANI	GGCC	6 cut(s)	295, 314, 943, 982, 1024, 1324
BspCNI	CTCAG	1 cut(s)	502
BspHI	TCATGA	2 cut(s)	1168, 1246
BspLI	GGNNCC	3 cut(s)	179, 225, 849
BspPI	GGATC	3 cut(s)	218, 231, 459
BspT104I	TTCGAA	1 cut(s)	1334
BsrBI	CCGCTC	1 cut(s)	900
BsrFI	RCCGGY	2 cut(s)	214, 1455
BsrI	ACTGG	2 cut(s)	451, 1273
BssAI	RCCGGY	2 cut(s)	214, 1455
BssECI	CCNNGG	2 cut(s)	321, 1325
BssMI	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
BssSI	CACGAG	1 cut(s)	1431
BssT1I	CCWWGG	2 cut(s)	321, 1325
Bst2BI	CACGAG	1 cut(s)	1431
Bst2UI	CCWGG	3 cut(s)	108, 255, 979
Bst4CI	ACNGT	5 cut(s)	157, 924, 1099, 1393, 1414
Bst6I	CTCTTC	1 cut(s)	129
BstAPI	GCANNNNNTGC	1 cut(s)	1115
BstBAI	YACGTR	1 cut(s)	565
BstBI	TTCGAA	1 cut(s)	1334
BstC8I	GCNNGC	3 cut(s)	216, 748, 794
BstDEI	CTNAG	2 cut(s)	510, 986
BstDSI	CCRYGG	1 cut(s)	1325
BstF5I	GGATG	5 cut(s)	263, 667, 711, 1009, 1318
BstKTI	GATC	6 cut(s)	226, 454, 727, 1188, 1246, 1292
BstMAI	GTCTC	2 cut(s)	425, 926
BstMBI	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
BstMWI	GCNNNNNNNGC	3 cut(s)	273, 861, 1115
BstNI	CCWGG	3 cut(s)	108, 255, 979
BstPAI	GACNNNNGTC	1 cut(s)	165
BstSCI	CCNGG	3 cut(s)	106, 253, 977
BstSLI	GKGCMC	1 cut(s)	1228
BstX2I	RGATCY	2 cut(s)	223, 451
BstXI	CCANNNNNNTGG	3 cut(s)	107, 170, 360
BstYI	RGATCY	2 cut(s)	223, 451
BsuI	GTATCC	1 cut(s)	106
BsuRI	GGCC	6 cut(s)	295, 314, 943, 982, 1024, 1324
BtgI	CCRYGG	1 cut(s)	1325
BtsCI	GGATG	5 cut(s)	263, 667, 711, 1009, 1318
BtsI	GCAGTG	1 cut(s)	1206
BtsIMutI	CAGTG	3 cut(s)	121, 1206, 1266
Cac8I	GCNNGC	3 cut(s)	216, 748, 794
CciI	TCATGA	2 cut(s)	1168, 1246
Cfr10I	RCCGGY	2 cut(s)	214, 1455
Cfr13I	GGNCC	2 cut(s)	251, 293
CseI	GACGC	1 cut(s)	580
Csp6I	GTAC	3 cut(s)	1192, 1354, 1423
CviAII	CATG	9 cut(s)	170, 290, 852, 1115, 1134, 1169, 1247, 1281, 1326
CviQI	GTAC	3 cut(s)	1192, 1354, 1423
DdeI	CTNAG	2 cut(s)	510, 986
DpnI	GATC	6 cut(s)	225, 453, 726, 1187, 1245, 1291
DpnII	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
DraIII	CACNNNGTG	1 cut(s)	747
EaeI	YGGCCR	1 cut(s)	1022
Eam1104I	CTCTTC	1 cut(s)	129
EarI	CTCTTC	1 cut(s)	129
EciI	GGCGGA	1 cut(s)	233
Ecl136II	GAGCTC	1 cut(s)	992
Eco130I	CCWWGG	2 cut(s)	321, 1325
Eco24I	GRGCYC	1 cut(s)	994
Eco32I	GATATC	1 cut(s)	717
Eco47I	GGWCC	1 cut(s)	251
Eco53kI	GAGCTC	1 cut(s)	992
Eco57I	CTGAAG	1 cut(s)	603
EcoICRI	GAGCTC	1 cut(s)	992
EcoRII	CCWGG	3 cut(s)	106, 253, 977
EcoRV	GATATC	1 cut(s)	717
EcoT14I	CCWWGG	2 cut(s)	321, 1325
EcoT38I	GRGCYC	1 cut(s)	994
ErhI	CCWWGG	2 cut(s)	321, 1325
FaeI	CATG	9 cut(s)	173, 293, 855, 1118, 1137, 1172, 1250, 1284, 1329
FatI	CATG	9 cut(s)	169, 289, 851, 1114, 1133, 1168, 1246, 1280, 1325
FauNDI	CATATG	1 cut(s)	550
FbaI	TGATCA	2 cut(s)	1185, 1243
FblI	GTMKAC	1 cut(s)	561
FokI	GGATG	5 cut(s)	270, 674, 718, 1016, 1325
FriOI	GRGCYC	1 cut(s)	994
FspBI	CTAG	2 cut(s)	912, 1287
GsaI	CCCAGC	4 cut(s)	9, 147, 543, 1353
GsuI	CTGGAG	1 cut(s)	247
HaeIII	GGCC	6 cut(s)	295, 314, 943, 982, 1024, 1324
HapII	CCGG	6 cut(s)	215, 227, 555, 770, 1025, 1456
HgaI	GACGC	1 cut(s)	580
Hin1II	CATG	9 cut(s)	173, 293, 855, 1118, 1137, 1172, 1250, 1284, 1329
HincII	GTYRAC	3 cut(s)	160, 739, 808
HindII	GTYRAC	3 cut(s)	160, 739, 808
HindIII	AAGCTT	1 cut(s)	794
HpaI	GTTAAC	1 cut(s)	739
HpaII	CCGG	6 cut(s)	215, 227, 555, 770, 1025, 1456
HphI	GGTGA	4 cut(s)	784, 941, 1253, 1372
Hpy166II	GTNNAC	6 cut(s)	160, 251, 562, 739, 808, 1356
Hpy188I	TCNGA	6 cut(s)	205, 333, 469, 583, 721, 956
Hpy188III	TCNNGA	9 cut(s)	52, 389, 509, 845, 1169, 1176, 1247, 1404, 1418
Hpy8I	GTNNAC	6 cut(s)	160, 251, 562, 739, 808, 1356
Hpy99I	CGWCG	3 cut(s)	200, 574, 924
HpyAV	CCTTC	4 cut(s)	138, 200, 361, 1099
HpyCH4III	ACNGT	5 cut(s)	157, 924, 1099, 1393, 1414
HpyCH4IV	ACGT	1 cut(s)	564
HpyCH4V	TGCA	5 cut(s)	89, 698, 1118, 1146, 1211
HpyF10VI	GCNNNNNNNGC	3 cut(s)	273, 861, 1115
HpyF3I	CTNAG	2 cut(s)	510, 986
HpySE526I	ACGT	1 cut(s)	564
Hsp92II	CATG	9 cut(s)	173, 293, 855, 1118, 1137, 1172, 1250, 1284, 1329
KroI	GCCGGC	1 cut(s)	214
KroNI	GCCGGC	1 cut(s)	216
Ksp22I	TGATCA	2 cut(s)	1185, 1243
KspAI	GTTAAC	1 cut(s)	739
Kzo9I	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
LmnI	GCTCC	4 cut(s)	82, 266, 997, 1027
LweI	GCATC	2 cut(s)	248, 592
MaeI	CTAG	2 cut(s)	912, 1287
MaeII	ACGT	1 cut(s)	564
MaeIII	GTNAC	6 cut(s)	151, 610, 655, 677, 889, 1364
MalI	GATC	6 cut(s)	225, 453, 726, 1187, 1245, 1291
MbiI	CCGCTC	1 cut(s)	900
MboI	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
MboII	GAAGA	3 cut(s)	116, 570, 833
MflI	RGATCY	2 cut(s)	223, 451
MhlI	GDGCHC	2 cut(s)	994, 1228
MluCI	AATT	8 cut(s)	39, 301, 597, 668, 781, 1139, 1155, 1160
MlyI	GAGTC	4 cut(s)	175, 386, 554, 567
MmeI	TCCRAC	4 cut(s)	388, 399, 546, 1040
MroNI	GCCGGC	1 cut(s)	214
MroXI	GAANNNNTTC	2 cut(s)	182, 1047
MseI	TTAA	5 cut(s)	38, 348, 738, 798, 1059
MspI	CCGG	6 cut(s)	215, 227, 555, 770, 1025, 1456
MspR9I	CCNGG	3 cut(s)	108, 255, 979
MvaI	CCWGG	3 cut(s)	108, 255, 979
MwoI	GCNNNNNNNGC	3 cut(s)	273, 861, 1115
NaeI	GCCGGC	1 cut(s)	216
NcoI	CCATGG	1 cut(s)	1325
NdeI	CATATG	1 cut(s)	550
NdeII	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
NgoMIV	GCCGGC	1 cut(s)	214
NlaIII	CATG	9 cut(s)	173, 293, 855, 1118, 1137, 1172, 1250, 1284, 1329
NlaIV	GGNNCC	3 cut(s)	179, 225, 849
NmuCI	GTSAC	1 cut(s)	151
NspV	TTCGAA	1 cut(s)	1334
PagI	TCATGA	2 cut(s)	1168, 1246
PdiI	GCCGGC	1 cut(s)	216
PdmI	GAANNNNTTC	2 cut(s)	182, 1047
PfeI	GAWTC	6 cut(s)	103, 111, 200, 470, 869, 1172
PfoI	TCCNGGA	1 cut(s)	106
PleI	GAGTC	4 cut(s)	174, 386, 553, 566
PpsI	GAGTC	4 cut(s)	174, 386, 553, 566
Ppu21I	YACGTR	1 cut(s)	565
PshAI	GACNNNNGTC	1 cut(s)	165
PshBI	ATTAAT	2 cut(s)	38, 1059
PsiI	TTATAA	1 cut(s)	1302
Psp124BI	GAGCTC	1 cut(s)	994
Psp6I	CCWGG	3 cut(s)	106, 253, 977
PspFI	CCCAGC	4 cut(s)	5, 143, 539, 1349
PspGI	CCWGG	3 cut(s)	106, 253, 977
PspN4I	GGNNCC	3 cut(s)	179, 225, 849
PspPI	GGNCC	2 cut(s)	251, 293
PsrI	GAACNNNNNNTAC	4 cut(s)	170, 202, 603, 635
PsuI	RGATCY	2 cut(s)	223, 451
RsaI	GTAC	3 cut(s)	1193, 1355, 1424
RsaNI	GTAC	3 cut(s)	1192, 1354, 1423
SacI	GAGCTC	1 cut(s)	994
SaqAI	TTAA	5 cut(s)	38, 348, 738, 798, 1059
Sau3AI	GATC	6 cut(s)	223, 451, 724, 1185, 1243, 1289
Sau96I	GGNCC	2 cut(s)	251, 293
SchI	GAGTC	4 cut(s)	175, 386, 554, 567
ScrFI	CCNGG	3 cut(s)	108, 255, 979
SduI	GDGCHC	2 cut(s)	994, 1228
SfaNI	GCATC	2 cut(s)	248, 592
SfuI	TTCGAA	1 cut(s)	1334
SinI	GGWCC	1 cut(s)	251
SmlI	CTYRAG	2 cut(s)	52, 488
SmoI	CTYRAG	2 cut(s)	52, 488
Sse9I	AATT	8 cut(s)	39, 301, 597, 668, 781, 1139, 1155, 1160
SsiI	CCGC	4 cut(s)	218, 750, 900, 1239
SspI	AATATT	1 cut(s)	15
SspMI	CTAG	2 cut(s)	912, 1287
SstI	GAGCTC	1 cut(s)	994
StyD4I	CCNGG	3 cut(s)	106, 253, 977
StyI	CCWWGG	2 cut(s)	321, 1325
TaaI	ACNGT	5 cut(s)	157, 924, 1099, 1393, 1414
TaiI	ACGT	1 cut(s)	567
TaqI	TCGA	6 cut(s)	198, 365, 672, 872, 919, 1334
TasI	AATT	8 cut(s)	39, 301, 597, 668, 781, 1139, 1155, 1160
TatI	WGTACW	1 cut(s)	1422
TfiI	GAWTC	6 cut(s)	103, 111, 200, 470, 869, 1172
Tru1I	TTAA	5 cut(s)	38, 348, 738, 798, 1059
Tru9I	TTAA	5 cut(s)	38, 348, 738, 798, 1059
TscAI	CASTG	3 cut(s)	121, 1213, 1273
TseFI	GTSAC	1 cut(s)	151
Tsp45I	GTSAC	1 cut(s)	151
TspDTI	ATGAA	3 cut(s)	456, 1122, 1329
TspGWI	ACGGA	5 cut(s)	674, 681, 1041, 1139, 1353
TspRI	CASTG	3 cut(s)	121, 1213, 1273
VpaK11BI	GGWCC	1 cut(s)	251
VspI	ATTAAT	2 cut(s)	38, 1059
XapI	RAATTY	2 cut(s)	668, 1160
XmiI	GTMKAC	1 cut(s)	561
XmnI	GAANNNNTTC	2 cut(s)	182, 1047
XspI	CTAG	2 cut(s)	912, 1287

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.