Belongs to the peptidase S10 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1802, 1856 |
| AccBSI | CCGCTC | 1 cut(s) | 228 |
| AciI | CCGC | 7 cut(s) | 228, 239, 755, 1771, 1774, 1844, 1847 |
| AclWI | GGATC | 2 cut(s) | 282, 1843 |
| AcoI | YGGCCR | 1 cut(s) | 426 |
| AcsI | RAATTY | 3 cut(s) | 522, 559, 1233 |
| AcuI | CTGAAG | 2 cut(s) | 254, 945 |
| AfaI | GTAC | 3 cut(s) | 972, 994, 1502 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 218, 1186, 1802, 1856 |
| AflII | CTTAAG | 1 cut(s) | 805 |
| AgsI | TTSAA | 8 cut(s) | 68, 364, 442, 493, 536, 615, 1048, 1127 |
| AjnI | CCWGG | 1 cut(s) | 1657 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 422, 454, 536, 568 |
| AleI | CACNNNNGTG | 1 cut(s) | 1882 |
| Alw21I | GWGCWC | 2 cut(s) | 1415, 1456 |
| Alw26I | GTCTC | 1 cut(s) | 719 |
| AlwI | GGATC | 2 cut(s) | 282, 1843 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 277, 556, 758 |
| AoxI | GGCC | 3 cut(s) | 229, 377, 426 |
| ApeKI | GCWGC | 5 cut(s) | 752, 1081, 1277, 1439, 1481 |
| ApoI | RAATTY | 3 cut(s) | 522, 559, 1233 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 466, 498, 692, 724 |
| AseI | ATTAAT | 1 cut(s) | 300 |
| AspS9I | GGNCC | 2 cut(s) | 230, 377 |
| AsuC2I | CCSGG | 2 cut(s) | 213, 835 |
| AsuHPI | GGTGA | 6 cut(s) | 403, 530, 734, 883, 1048, 1577 |
| BaeGI | GKGCMC | 2 cut(s) | 309, 543 |
| BalI | TGGCCA | 1 cut(s) | 428 |
| BanII | GRGCYC | 2 cut(s) | 1415, 1456 |
| Bbv12I | GWGCWC | 2 cut(s) | 1415, 1456 |
| BbvCI | CCTCAGC | 2 cut(s) | 666, 966 |
| BbvI | GCAGC | 5 cut(s) | 764, 1093, 1289, 1426, 1493 |
| BccI | CCATC | 5 cut(s) | 818, 1159, 1556, 1771, 1804 |
| BceAI | ACGGC | 1 cut(s) | 185 |
| BciT130I | CCWGG | 1 cut(s) | 1659 |
| BciVI | GTATCC | 2 cut(s) | 1243, 1665 |
| BclI | TGATCA | 1 cut(s) | 96 |
| BcnI | CCSGG | 2 cut(s) | 213, 835 |
| BcoDI | GTCTC | 1 cut(s) | 719 |
| BfaI | CTAG | 1 cut(s) | 737 |
| BfmI | CTRYAG | 1 cut(s) | 602 |
| BfrI | CTTAAG | 1 cut(s) | 805 |
| BfuI | GTATCC | 2 cut(s) | 1243, 1665 |
| BisI | GCNGC | 9 cut(s) | 229, 753, 756, 1082, 1278, 1440, 1482, 1772, 1847 |
| BlsI | GCNGC | 9 cut(s) | 230, 754, 757, 1083, 1279, 1441, 1483, 1773, 1848 |
| Bme1390I | CCNGG | 3 cut(s) | 213, 835, 1659 |
| BmgT120I | GGNCC | 2 cut(s) | 230, 377 |
| BmiI | GGNNCC | 2 cut(s) | 672, 1422 |
| BmrFI | CCNGG | 3 cut(s) | 213, 835, 1659 |
| BmrI | ACTGGG | 1 cut(s) | 56 |
| BmsI | GCATC | 3 cut(s) | 880, 1009, 1728 |
| BmuI | ACTGGG | 1 cut(s) | 56 |
| BpmI | CTGGAG | 3 cut(s) | 183, 962, 1440 |
| Bpu10I | CCTNAGC | 2 cut(s) | 666, 966 |
| BpuEI | CTTGAG | 1 cut(s) | 1115 |
| BpuMI | CCSGG | 2 cut(s) | 213, 835 |
| BsaJI | CCNNGG | 2 cut(s) | 1327, 1831 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 935, 965, 1512, 1542 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 218, 1186, 1802, 1856 |
| Bse1I | ACTGG | 6 cut(s) | 62, 161, 166, 1422, 1540, 1850 |
| Bse3DI | GCAATG | 2 cut(s) | 380, 731 |
| BseBI | CCWGG | 1 cut(s) | 1659 |
| BseDI | CCNNGG | 2 cut(s) | 1327, 1831 |
| BseGI | GGATG | 6 cut(s) | 926, 1024, 1057, 1548, 1750, 1782 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 218, 1186, 1802, 1856 |
| BseMI | GCAATG | 2 cut(s) | 380, 731 |
| BseNI | ACTGG | 6 cut(s) | 62, 161, 166, 1422, 1540, 1850 |
| BseRI | GAGGAG | 4 cut(s) | 895, 1236, 1520, 1628 |
| BseSI | GKGCMC | 2 cut(s) | 309, 543 |
| BseXI | GCAGC | 5 cut(s) | 764, 1093, 1289, 1426, 1493 |
| BseYI | CCCAGC | 1 cut(s) | 25 |
| BsgI | GTGCAG | 1 cut(s) | 198 |
| BshFI | GGCC | 3 cut(s) | 231, 379, 428 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1415, 1456 |
| BsiSI | CCGG | 4 cut(s) | 213, 662, 834, 863 |
| BslFI | GGGAC | 2 cut(s) | 172, 997 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 218, 1186, 1802, 1856 |
| BsmAI | GTCTC | 1 cut(s) | 719 |
| BsmFI | GGGAC | 2 cut(s) | 172, 997 |
| BsmI | GAATGC | 2 cut(s) | 450, 1722 |
| BsnI | GGCC | 3 cut(s) | 231, 379, 428 |
| Bsp1286I | GDGCHC | 4 cut(s) | 309, 543, 1415, 1456 |
| Bsp1407I | TGTACA | 1 cut(s) | 1500 |
| Bsp143I | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| Bsp19I | CCATGG | 1 cut(s) | 1327 |
| BspACI | CCGC | 7 cut(s) | 228, 239, 755, 1771, 1774, 1844, 1847 |
| BspANI | GGCC | 3 cut(s) | 231, 379, 428 |
| BspLI | GGNNCC | 2 cut(s) | 672, 1422 |
| BspMAI | CTGCAG | 1 cut(s) | 606 |
| BspPI | GGATC | 2 cut(s) | 282, 1843 |
| BspTI | CTTAAG | 1 cut(s) | 805 |
| BsrBI | CCGCTC | 1 cut(s) | 228 |
| BsrDI | GCAATG | 2 cut(s) | 380, 731 |
| BsrGI | TGTACA | 1 cut(s) | 1500 |
| BsrI | ACTGG | 6 cut(s) | 62, 161, 166, 1422, 1540, 1850 |
| BssECI | CCNNGG | 2 cut(s) | 1327, 1831 |
| BssMI | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| BssT1I | CCWWGG | 1 cut(s) | 1327 |
| Bst2UI | CCWGG | 1 cut(s) | 1659 |
| Bst4CI | ACNGT | 3 cut(s) | 415, 1214, 1371 |
| Bst6I | CTCTTC | 2 cut(s) | 1034, 1213 |
| BstAFI | CTTAAG | 1 cut(s) | 805 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 82, 1490 |
| BstAUI | TGTACA | 1 cut(s) | 1500 |
| BstC8I | GCNNGC | 2 cut(s) | 78, 1491 |
| BstDSI | CCRYGG | 1 cut(s) | 1327 |
| BstEII | GGTNACC | 1 cut(s) | 1445 |
| BstF5I | GGATG | 6 cut(s) | 926, 1024, 1057, 1548, 1750, 1782 |
| BstKTI | GATC | 6 cut(s) | 99, 290, 940, 1726, 1814, 1838 |
| BstMAI | GTCTC | 1 cut(s) | 719 |
| BstMBI | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 82, 547, 1004, 1274, 1490, 1726 |
| BstNI | CCWGG | 1 cut(s) | 1659 |
| BstNSI | RCATGY | 1 cut(s) | 786 |
| BstPI | GGTNACC | 1 cut(s) | 1445 |
| BstSCI | CCNGG | 3 cut(s) | 211, 833, 1657 |
| BstSFI | CTRYAG | 1 cut(s) | 602 |
| BstSLI | GKGCMC | 2 cut(s) | 309, 543 |
| BstV1I | GCAGC | 5 cut(s) | 764, 1093, 1289, 1426, 1493 |
| BstX2I | RGATCY | 1 cut(s) | 287 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 161 |
| BstYI | RGATCY | 1 cut(s) | 287 |
| BsuI | GTATCC | 2 cut(s) | 1243, 1665 |
| BsuRI | GGCC | 3 cut(s) | 231, 379, 428 |
| BtgI | CCRYGG | 1 cut(s) | 1327 |
| BtsCI | GGATG | 6 cut(s) | 926, 1024, 1057, 1548, 1750, 1782 |
| BtsI | GCAGTG | 2 cut(s) | 181, 627 |
| BtsIMutI | CAGTG | 5 cut(s) | 154, 181, 627, 1415, 1533 |
| Cac8I | GCNNGC | 2 cut(s) | 78, 1491 |
| CaiI | CAGNNNCTG | 3 cut(s) | 277, 556, 758 |
| Cfr13I | GGNCC | 2 cut(s) | 230, 377 |
| Csp6I | GTAC | 3 cut(s) | 971, 993, 1501 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1011, 1046 |
| CviAII | CATG | 5 cut(s) | 47, 191, 783, 1112, 1328 |
| CviQI | GTAC | 3 cut(s) | 971, 993, 1501 |
| DpnI | GATC | 6 cut(s) | 98, 289, 939, 1725, 1813, 1837 |
| DpnII | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| EaeI | YGGCCR | 1 cut(s) | 426 |
| Eam1104I | CTCTTC | 2 cut(s) | 1034, 1213 |
| EarI | CTCTTC | 2 cut(s) | 1034, 1213 |
| EciI | GGCGGA | 1 cut(s) | 1789 |
| Ecl136II | GAGCTC | 2 cut(s) | 1413, 1454 |
| Eco130I | CCWWGG | 1 cut(s) | 1327 |
| Eco24I | GRGCYC | 2 cut(s) | 1415, 1456 |
| Eco53kI | GAGCTC | 2 cut(s) | 1413, 1454 |
| Eco57I | CTGAAG | 2 cut(s) | 254, 945 |
| Eco91I | GGTNACC | 1 cut(s) | 1445 |
| EcoICRI | GAGCTC | 2 cut(s) | 1413, 1454 |
| EcoO65I | GGTNACC | 1 cut(s) | 1445 |
| EcoRI | GAATTC | 1 cut(s) | 1233 |
| EcoRII | CCWGG | 1 cut(s) | 1657 |
| EcoT14I | CCWWGG | 1 cut(s) | 1327 |
| EcoT22I | ATGCAT | 1 cut(s) | 934 |
| EcoT38I | GRGCYC | 2 cut(s) | 1415, 1456 |
| ErhI | CCWWGG | 1 cut(s) | 1327 |
| FaeI | CATG | 5 cut(s) | 50, 194, 786, 1115, 1331 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 65, 97, 69, 101, 813, 845, 1058, 1090 |
| FaqI | GGGAC | 2 cut(s) | 172, 997 |
| FatI | CATG | 5 cut(s) | 46, 190, 782, 1111, 1327 |
| FauNDI | CATATG | 1 cut(s) | 1086 |
| FbaI | TGATCA | 1 cut(s) | 96 |
| Fnu4HI | GCNGC | 9 cut(s) | 229, 753, 756, 1082, 1278, 1440, 1482, 1772, 1847 |
| FokI | GGATG | 6 cut(s) | 933, 1031, 1064, 1535, 1757, 1789 |
| FriOI | GRGCYC | 2 cut(s) | 1415, 1456 |
| Fsp4HI | GCNGC | 9 cut(s) | 229, 753, 756, 1082, 1278, 1440, 1482, 1772, 1847 |
| FspBI | CTAG | 1 cut(s) | 737 |
| GluI | GCNGC | 9 cut(s) | 229, 753, 756, 1082, 1278, 1440, 1482, 1772, 1847 |
| GsaI | CCCAGC | 1 cut(s) | 29 |
| GsuI | CTGGAG | 3 cut(s) | 183, 962, 1440 |
| HaeIII | GGCC | 3 cut(s) | 231, 379, 428 |
| HapII | CCGG | 4 cut(s) | 213, 662, 834, 863 |
| Hin1II | CATG | 5 cut(s) | 50, 194, 786, 1115, 1331 |
| HindIII | AAGCTT | 2 cut(s) | 716, 1319 |
| HpaII | CCGG | 4 cut(s) | 213, 662, 834, 863 |
| HphI | GGTGA | 6 cut(s) | 403, 530, 734, 883, 1048, 1577 |
| Hpy166II | GTNNAC | 3 cut(s) | 1501, 1579, 1620 |
| Hpy188III | TCNNGA | 9 cut(s) | 40, 455, 527, 556, 887, 941, 1457, 1610, 1803 |
| Hpy8I | GTNNAC | 3 cut(s) | 1501, 1579, 1620 |
| HpyAV | CCTTC | 6 cut(s) | 324, 804, 841, 862, 1042, 1063 |
| HpyCH4III | ACNGT | 3 cut(s) | 415, 1214, 1371 |
| HpyCH4IV | ACGT | 2 cut(s) | 1335, 1767 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 82, 547, 1004, 1274, 1490, 1726 |
| HpySE526I | ACGT | 2 cut(s) | 1335, 1767 |
| Hsp92II | CATG | 5 cut(s) | 50, 194, 786, 1115, 1331 |
| Ksp22I | TGATCA | 1 cut(s) | 96 |
| Kzo9I | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| LmnI | GCTCC | 6 cut(s) | 447, 688, 1191, 1274, 1426, 1459 |
| Lsp1109I | GCAGC | 5 cut(s) | 764, 1093, 1289, 1426, 1493 |
| LweI | GCATC | 3 cut(s) | 880, 1009, 1728 |
| MaeI | CTAG | 1 cut(s) | 737 |
| MaeII | ACGT | 2 cut(s) | 1335, 1767 |
| MaeIII | GTNAC | 9 cut(s) | 149, 166, 632, 923, 1206, 1445, 1462, 1518, 1686 |
| MalI | GATC | 6 cut(s) | 98, 289, 939, 1725, 1813, 1837 |
| MbiI | CCGCTC | 1 cut(s) | 228 |
| MboI | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| MboII | GAAGA | 8 cut(s) | 80, 833, 1051, 1078, 1230, 1682, 1765, 1791 |
| MfeI | CAATTG | 1 cut(s) | 124 |
| MflI | RGATCY | 1 cut(s) | 287 |
| MhlI | GDGCHC | 4 cut(s) | 309, 543, 1415, 1456 |
| MlsI | TGGCCA | 1 cut(s) | 428 |
| MluCI | AATT | 7 cut(s) | 124, 359, 522, 559, 655, 741, 1233 |
| MluNI | TGGCCA | 1 cut(s) | 428 |
| MlyI | GAGTC | 4 cut(s) | 268, 467, 469, 892 |
| MmeI | TCCRAC | 2 cut(s) | 591, 1837 |
| Mox20I | TGGCCA | 1 cut(s) | 428 |
| Mph1103I | ATGCAT | 1 cut(s) | 934 |
| MscI | TGGCCA | 1 cut(s) | 428 |
| MseI | TTAA | 4 cut(s) | 300, 806, 1056, 1107 |
| MslI | CAYNNNNRTG | 4 cut(s) | 51, 536, 1116, 1882 |
| Msp20I | TGGCCA | 1 cut(s) | 428 |
| MspA1I | CMGCKG | 2 cut(s) | 755, 965 |
| MspCI | CTTAAG | 1 cut(s) | 805 |
| MspI | CCGG | 4 cut(s) | 213, 662, 834, 863 |
| MspR9I | CCNGG | 3 cut(s) | 213, 835, 1659 |
| MunI | CAATTG | 1 cut(s) | 124 |
| Mva1269I | GAATGC | 2 cut(s) | 450, 1722 |
| MvaI | CCWGG | 1 cut(s) | 1659 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 82, 547, 1004, 1274, 1490, 1726 |
| NciI | CCSGG | 2 cut(s) | 213, 835 |
| NcoI | CCATGG | 1 cut(s) | 1327 |
| NdeI | CATATG | 1 cut(s) | 1086 |
| NdeII | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| NlaIII | CATG | 5 cut(s) | 50, 194, 786, 1115, 1331 |
| NlaIV | GGNNCC | 2 cut(s) | 672, 1422 |
| NmuCI | GTSAC | 4 cut(s) | 149, 166, 923, 1462 |
| NsiI | ATGCAT | 1 cut(s) | 934 |
| NspI | RCATGY | 1 cut(s) | 786 |
| OliI | CACNNNNGTG | 1 cut(s) | 1882 |
| PctI | GAATGC | 2 cut(s) | 450, 1722 |
| PfeI | GAWTC | 6 cut(s) | 43, 216, 493, 700, 1287, 1789 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1802, 1856 |
| PfoI | TCCNGGA | 1 cut(s) | 833 |
| PkrI | GCNGC | 9 cut(s) | 230, 754, 757, 1083, 1279, 1441, 1483, 1773, 1848 |
| PleI | GAGTC | 4 cut(s) | 268, 466, 469, 891 |
| PpsI | GAGTC | 4 cut(s) | 268, 466, 469, 891 |
| PshBI | ATTAAT | 1 cut(s) | 300 |
| Psp124BI | GAGCTC | 2 cut(s) | 1415, 1456 |
| Psp6I | CCWGG | 1 cut(s) | 1657 |
| PspEI | GGTNACC | 1 cut(s) | 1445 |
| PspFI | CCCAGC | 1 cut(s) | 25 |
| PspGI | CCWGG | 1 cut(s) | 1657 |
| PspN4I | GGNNCC | 2 cut(s) | 672, 1422 |
| PspPI | GGNCC | 2 cut(s) | 230, 377 |
| PstI | CTGCAG | 1 cut(s) | 606 |
| PstNI | CAGNNNCTG | 3 cut(s) | 277, 556, 758 |
| PsuI | RGATCY | 1 cut(s) | 287 |
| PvuII | CAGCTG | 1 cut(s) | 965 |
| RsaI | GTAC | 3 cut(s) | 972, 994, 1502 |
| RsaNI | GTAC | 3 cut(s) | 971, 993, 1501 |
| RseI | CAYNNNNRTG | 4 cut(s) | 51, 536, 1116, 1882 |
| SacI | GAGCTC | 2 cut(s) | 1415, 1456 |
| SaqAI | TTAA | 4 cut(s) | 300, 806, 1056, 1107 |
| SatI | GCNGC | 9 cut(s) | 229, 753, 756, 1082, 1278, 1440, 1482, 1772, 1847 |
| Sau3AI | GATC | 6 cut(s) | 96, 287, 937, 1723, 1811, 1835 |
| Sau96I | GGNCC | 2 cut(s) | 230, 377 |
| SchI | GAGTC | 4 cut(s) | 268, 467, 469, 892 |
| ScrFI | CCNGG | 3 cut(s) | 213, 835, 1659 |
| SduI | GDGCHC | 4 cut(s) | 309, 543, 1415, 1456 |
| SfaNI | GCATC | 3 cut(s) | 880, 1009, 1728 |
| SfcI | CTRYAG | 1 cut(s) | 602 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 51, 536, 1116, 1882 |
| SmlI | CTYRAG | 2 cut(s) | 805, 1130 |
| SmoI | CTYRAG | 2 cut(s) | 805, 1130 |
| Sse9I | AATT | 7 cut(s) | 124, 359, 522, 559, 655, 741, 1233 |
| SsiI | CCGC | 7 cut(s) | 228, 239, 755, 1771, 1774, 1844, 1847 |
| SspI | AATATT | 2 cut(s) | 1011, 1105 |
| SspMI | CTAG | 1 cut(s) | 737 |
| SstI | GAGCTC | 2 cut(s) | 1415, 1456 |
| StyD4I | CCNGG | 3 cut(s) | 211, 833, 1657 |
| StyI | CCWWGG | 1 cut(s) | 1327 |
| TaaI | ACNGT | 3 cut(s) | 415, 1214, 1371 |
| TaiI | ACGT | 2 cut(s) | 1338, 1770 |
| TaqI | TCGA | 4 cut(s) | 461, 878, 1524, 1871 |
| TasI | AATT | 7 cut(s) | 124, 359, 522, 559, 655, 741, 1233 |
| TatI | WGTACW | 1 cut(s) | 1500 |
| TauI | GCSGC | 4 cut(s) | 231, 758, 1774, 1849 |
| TfiI | GAWTC | 6 cut(s) | 43, 216, 493, 700, 1287, 1789 |
| Tru1I | TTAA | 4 cut(s) | 300, 806, 1056, 1107 |
| Tru9I | TTAA | 4 cut(s) | 300, 806, 1056, 1107 |
| TscAI | CASTG | 5 cut(s) | 161, 181, 627, 1422, 1540 |
| TseFI | GTSAC | 4 cut(s) | 149, 166, 923, 1462 |
| TseI | GCWGC | 5 cut(s) | 752, 1081, 1277, 1439, 1481 |
| Tsp45I | GTSAC | 4 cut(s) | 149, 166, 923, 1462 |
| TspDTI | ATGAA | 3 cut(s) | 35, 1128, 1586 |
| TspRI | CASTG | 5 cut(s) | 161, 181, 627, 1422, 1540 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1802, 1856 |
| Vha464I | CTTAAG | 1 cut(s) | 805 |
| VspI | ATTAAT | 1 cut(s) | 300 |
| XapI | RAATTY | 3 cut(s) | 522, 559, 1233 |
| XceI | RCATGY | 1 cut(s) | 786 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1559 |
| XspI | CTAG | 1 cut(s) | 737 |
| Zsp2I | ATGCAT | 1 cut(s) | 934 |