FvH4_6g05392

Benzyl alcohol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000190
Physical Location & Seq
Reverse (-)
3042305 .. 3044292
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05392.t1

Sequence Viewer

Length: 1209 bp
ATGTCGTTGCCATCCTCCCTAATATTTCAGGGTTATCGAATGCAACCACAACTTGTAACTCCGGCAAGGCCAACTCCTCGTGAAATAAAGATGCTGTCTGATATAGATGACCAGCAAGGCCTTAGGTTTCAGATACCAGCCATCATAGCCTACAAGAACAATCTTTCGATGTCGAACCGAAAAGACCCCGTTCTGGTGATCATAAGGGAAGCAATAAGTAGAGCATCGGTGTACTACTACCCTTTAGCTGGTAGGCTCAGGGAAGGGCCTAACAAAAAGCTTATGGTGGATTGCAATGGAGAAGGCGTCTTGTTCGTCGAGGCTAAAGCTGACGTCACTCTCGAGCAACTCGGAGATGCTATTCGACCCCCATGCCCTTTCTTAGAAGGTTTCATGTGTGATGTTCCCGGCTCTGATGGCATTCTTGGTTGCCCTTTGTTGTTATTTCAGGTGAGCATTTTGACATGTGGAGGTTTCATACTTGCATTGCGCCTCAACCATACAATGTGCTATGCGCCTAGATTGGTCCAGTTCTTGAACACCGTAGGGGAGATGGCTCAAGGAAAAGATGCACCATCTATTCCACCAGTGTGGAAGCGAGAGGAAATGAAGGCCTTGAAGAAACATCTTCCGCTACACCTTTCAGCTTGCTCCACATTTGGCCTAATGACATCTTGTTTATGGAAATGCCGCACAGCTGCACTTGAGCTTCATCCGAAACAGGTTGTTCGGGTTTCATGCTTAGTCAATGCACGGGGCAAGGGCAACAATCTACATCTTCCTTTGGGTTACTACGGCAATGCGTTCGCATACCCAGCTGCTGTTTCGGAAGTGAAACAACTGTGTGAGAGTTCGTTGGGATATGCAGTGGAGTTGGTGATGAAGGCAAAAGCTGAAGTGAATGAAGAGTACATGCGATCAGTGGCGGATCTTATGGTATTAAGAGGAAGGCCTCCATATACATCGACAGGGAACTTCCTAGTTTCAGATAATAGACATACTGGCTTTGGAGAGGTGAACTTGGGGTGGGAAAAGTCTGTATTTTCTGGACCTTCCAAGGCCTGGAGCAATATTAGTTTCTATATATGTTCAGCACAAAATGGAGAAAGAAGATGGAATTTCGGTGTCAGTGTGCTTGCCCTTGTGCAGTATGGAGAGATTCCAGCAGGAACTCGAGAAGATGACATCTCAGGAGATAGTGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

403

Amino Acids

44.35

Weight (kDa)

8.64

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 15 - 205 7.8e-39 Transferase family
Transferase PF02458 218 - 371 1.4e-17 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G03480 AT5G17540
fragaria_vesca FvH4_2g12510 FvH4_3g38880 FvH4_3g40890 FvH4_5g24240 FvH4_5g24250 FvH4_5g24270 FvH4_5g24273 FvH4_6g05391 FvH4_6g05392
malus_domestica MD00G1201900.v1.1 MD00G1202100.v1.1 MD02G1001400.v1.1 MD02G1013900.v1.1 MD02G1014800.v1.1 MD02G1015000.v1.1 MD02G1015200.v1.1 MD03G1080200.v1.1 MD06G1016200.v1.1 MD06G1016500.v1.1 MD06G1017700.v1.1 MD06G1018100.v1.1 MD06G1018200.v1.1 MD06G1018400.v1.1 MD06G1018700.v1.1 MD06G1018800.v1.1 MD06G1019000.v1.1 MD06G1019100.v1.1 MD06G1019300.v1.1 MD06G1019800.v1.1 MD06G1020400.v1.1 MD06G1020700.v1.1 MD06G1020800.v1.1 MD06G1020900.v1.1 MD12G1038300.v1.1 MD12G1039400.v1.1
prunus_persica Prupe.5G017800_v2.0.a1 Prupe.5G017900_v2.0.a1 Prupe.5G018000_v2.0.a1 Prupe.5G018100_v2.0.a1 Prupe.5G018200_v2.0.a1 Prupe.8G000100_v2.0.a1
pyrus_communis pycom02g01330 pycom02g01390 pycom03g06420 pycom06g01300 pycom06g01320 pycom06g01340 pycom06g01500 pycom06g01510 pycom06g01520 pycom12g03300 pycom12g03320
rosa_chinensis RchiOBHm_Chr5g0051841 RchiOBHm_Chr6g0272991 RchiOBHm_Chr7g0213951 RchiOBHm_Chr7g0214051 RchiOBHm_Chr7g0214131 RchiOBHm_Chr7g0214161 RchiOBHm_Chr7g0214231 RchiOBHm_Chr7g0214321 RchiOBHm_Chr7g0214351 RchiOBHm_Chr7g0214391 RchiOBHm_Chr7g0214451 RchiOBHm_Chr7g0222591
rosa_laevigata RLG00000002044 RLG00000002744 RLG00000002751 RLG00000002752 RLG00000002755 RLG00000002756 RLG00000002757 RLG00000002758 RLG00000002762 RLG00000002763 RLG00000013652
rosa_multiflora Rmu_sc0000328.1_g000008 Rmu_sc0003764.1_g000001 Rmu_sc0003764.1_g000002 Rmu_sc0004199.1_g000004 Rmu_sc0004199.1_g000006 Rmu_sc0004199.1_g000014 Rmu_sc0004199.1_g000019 Rmu_sc0004199.1_g000022 Rmu_sc0005063.1_g000018 Rmu_sc0006422.1_g000030 Rmu_sc0006422.1_g000031 Rmu_sc0009579.1_g000011 Rmu_sc0009579.1_g000017 Rmu_sc0016182.1_g000005 Rmu_sc0016182.1_g000007 Rmu_sc0016279.1_g000002 Rmu_ssc0000387.1_g000031
rosa_roxburghii Rroxscaffold_1G00027390 Rroxscaffold_2G00146810 Rroxscaffold_3G00236600 Rroxscaffold_3G00236620 Rroxscaffold_3G00245350 Rroxscaffold_3G00245400 Rroxscaffold_3G00245440 Rroxscaffold_3G00245480 Rroxscaffold_3G00245560 Rroxscaffold_7G00195770
rosa_rugosa Rorug02G0047800 Rorug02G0047900 Rorug05G0276800 Rorug06G0071900 Rorug07G0145400 Rorug07G0145500 Rorug07G0145600 Rorug07G0145700 Rorug07G0145700 Rorug07G0145800 Rorug07G0211200
rosa_samantha Rh5BG359700 Rh6AG187600 Rh6DG181500 Rh7AG278000 Rh7AG278900 Rh7AG279800 Rh7AG279900 Rh7AG280100 Rh7AG280400 Rh7AG280700 Rh7AG281200 Rh7AG354300 Rh7AG354800 Rh7BG272300 Rh7BG272500 Rh7BG272600 Rh7BG272700 Rh7BG272800 Rh7BG273000 Rh7BG343200 Rh7CG297000 Rh7CG297900 Rh7CG298100 Rh7CG298800 Rh7CG299100 Rh7CG299300 Rh7CG299400 Rh7CG300000 Rh7CG300200 Rh7CG300600 Rh7CG371800 Rh7CG371900
rosa_wichuraiana Rw5G032880 Rw6G016270 Rw7G023930 Rw7G023980 Rw7G023990 Rw7G024000 Rw7G024010 Rw7G024020 Rw7G024030 Rw7G029800 Rw7G030090 Rw7G030140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 336
AccB7I CCANNNNNTGG 1 cut(s) 1062
AciI CCGC 3 cut(s) 632, 691, 926
AclWI GGATC 1 cut(s) 936
AcsI RAATTY 1 cut(s) 1117
AcuI CTGAAG 1 cut(s) 915
AcyI GRCGYC 2 cut(s) 306, 333
AfaI GTAC 2 cut(s) 233, 911
AfiI CCNNNNNNNGG 3 cut(s) 193, 248, 1062
AflIII ACRYGT 1 cut(s) 464
AgsI TTSAA 2 cut(s) 538, 619
AjnI CCWGG 1 cut(s) 1061
AleI CACNNNNGTG 1 cut(s) 589
AluBI AGCT 8 cut(s) 248, 280, 329, 647, 698, 709, 818, 893
AluI AGCT 8 cut(s) 248, 280, 329, 647, 698, 709, 818, 893
AlwI GGATC 1 cut(s) 936
AlwNI CAGNNNCTG 1 cut(s) 821
Ama87I CYCGRG 2 cut(s) 341, 1173
AoxI GGCC 7 cut(s) 68, 118, 266, 612, 661, 950, 1059
ApeKI GCWGC 2 cut(s) 698, 818
ApoI RAATTY 1 cut(s) 1117
AspLEI GCGC 2 cut(s) 492, 517
AspS9I GGNCC 3 cut(s) 266, 526, 1049
AsuC2I CCSGG 1 cut(s) 408
AsuHPI GGTGA 4 cut(s) 208, 463, 889, 1027
AvaI CYCGRG 2 cut(s) 341, 1173
AvaII GGWCC 2 cut(s) 526, 1049
AxyI CCTNAGG 1 cut(s) 122
BauI CACGAG 1 cut(s) 78
BbvI GCAGC 2 cut(s) 685, 805
BccI CCATC 6 cut(s) 19, 149, 410, 547, 583, 1107
BceAI ACGGC 1 cut(s) 811
BcgI CGANNNNNNTGC 4 cut(s) 354, 388, 787, 821
BciT130I CCWGG 1 cut(s) 1063
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 1 cut(s) 408
BfaI CTAG 2 cut(s) 519, 980
BisI GCNGC 3 cut(s) 691, 699, 819
BlsI GCNGC 3 cut(s) 692, 700, 820
Bme1390I CCNGG 2 cut(s) 408, 1063
Bme18I GGWCC 2 cut(s) 526, 1049
BmeT110I CYCGRG 2 cut(s) 341, 1173
BmgT120I GGNCC 3 cut(s) 266, 526, 1049
BmrFI CCNGG 2 cut(s) 408, 1063
BmsI GCATC 4 cut(s) 81, 233, 346, 559
BpmI CTGGAG 1 cut(s) 1084
Bpu10I CCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 543, 725
BpuMI CCSGG 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 306, 333
BsaJI CCNNGG 1 cut(s) 1056
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 248, 1062
Bse1I ACTGG 3 cut(s) 529, 587, 1006
Bse21I CCTNAGG 1 cut(s) 122
Bse3DI GCAATG 3 cut(s) 301, 485, 805
BseBI CCWGG 1 cut(s) 1063
BseDI CCNNGG 1 cut(s) 1056
BseGI GGATG 2 cut(s) 11, 712
BseLI CCNNNNNNNGG 3 cut(s) 193, 248, 1062
BseMI GCAATG 3 cut(s) 301, 485, 805
BseMII CTCAG 2 cut(s) 271, 1203
BseNI ACTGG 3 cut(s) 529, 587, 1006
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 685, 805
BseYI CCCAGC 1 cut(s) 814
BsgI GTGCAG 2 cut(s) 684, 1166
BshFI GGCC 7 cut(s) 70, 120, 268, 614, 663, 952, 1061
BsiHKCI CYCGRG 2 cut(s) 341, 1173
BsiSI CCGG 2 cut(s) 62, 408
BslI CCNNNNNNNGG 3 cut(s) 193, 248, 1062
BsmI GAATGC 2 cut(s) 45, 420
BsnI GGCC 7 cut(s) 70, 120, 268, 614, 663, 952, 1061
BsoBI CYCGRG 2 cut(s) 341, 1173
Bsp143I GATC 3 cut(s) 198, 917, 928
BspACI CCGC 3 cut(s) 632, 691, 926
BspANI GGCC 7 cut(s) 70, 120, 268, 614, 663, 952, 1061
BspCNI CTCAG 2 cut(s) 270, 1202
BspPI GGATC 1 cut(s) 936
BsrDI GCAATG 3 cut(s) 301, 485, 805
BsrI ACTGG 3 cut(s) 529, 587, 1006
BssECI CCNNGG 1 cut(s) 1056
BssMI GATC 3 cut(s) 198, 917, 928
BssNI GRCGYC 2 cut(s) 306, 333
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 1063
Bst4CI ACNGT 2 cut(s) 544, 843
Bst6I CTCTTC 1 cut(s) 900
BstACI GRCGYC 2 cut(s) 306, 333
BstC8I GCNNGC 2 cut(s) 649, 1137
BstDEI CTNAG 5 cut(s) 122, 257, 382, 742, 1189
BstF5I GGATG 2 cut(s) 11, 712
BstHHI GCGC 2 cut(s) 492, 517
BstKTI GATC 3 cut(s) 201, 920, 931
BstMBI GATC 3 cut(s) 198, 917, 928
BstMWI GCNNNNNNNGC 3 cut(s) 146, 417, 815
BstNI CCWGG 1 cut(s) 1063
BstNSI RCATGY 2 cut(s) 468, 916
BstSCI CCNGG 2 cut(s) 406, 1061
BstV1I GCAGC 2 cut(s) 685, 805
BstX2I RGATCY 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 591
BstYI RGATCY 1 cut(s) 928
Bsu36I CCTNAGG 1 cut(s) 122
BsuRI GGCC 7 cut(s) 70, 120, 268, 614, 663, 952, 1061
BtsCI GGATG 2 cut(s) 11, 712
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 4 cut(s) 594, 873, 927, 1135
Cac8I GCNNGC 2 cut(s) 649, 1137
CaiI CAGNNNCTG 1 cut(s) 821
CfoI GCGC 2 cut(s) 492, 517
Cfr13I GGNCC 3 cut(s) 266, 526, 1049
CseI GACGC 1 cut(s) 295
Csp6I GTAC 2 cut(s) 232, 910
CviAII CATG 5 cut(s) 372, 394, 465, 738, 913
CviQI GTAC 2 cut(s) 232, 910
DdeI CTNAG 5 cut(s) 122, 257, 382, 742, 1189
DpnI GATC 3 cut(s) 200, 919, 930
DpnII GATC 3 cut(s) 198, 917, 928
Eam1104I CTCTTC 1 cut(s) 900
EarI CTCTTC 1 cut(s) 900
EciI GGCGGA 1 cut(s) 941
Eco130I CCWWGG 1 cut(s) 1056
Eco147I AGGCCT 4 cut(s) 120, 614, 952, 1061
Eco47I GGWCC 2 cut(s) 526, 1049
Eco57I CTGAAG 1 cut(s) 915
Eco81I CCTNAGG 1 cut(s) 122
Eco88I CYCGRG 2 cut(s) 341, 1173
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 1061
EcoT14I CCWWGG 1 cut(s) 1056
ErhI CCWWGG 1 cut(s) 1056
FaeI CATG 5 cut(s) 375, 397, 468, 741, 916
FatI CATG 5 cut(s) 371, 393, 464, 737, 912
FbaI TGATCA 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 691, 699, 819
FokI GGATG 1 cut(s) 699
Fsp4HI GCNGC 3 cut(s) 691, 699, 819
FspBI CTAG 2 cut(s) 519, 980
GlaI GCGC 2 cut(s) 491, 516
GluI GCNGC 3 cut(s) 691, 699, 819
GsaI CCCAGC 1 cut(s) 818
GsuI CTGGAG 1 cut(s) 1084
HaeIII GGCC 7 cut(s) 70, 120, 268, 614, 663, 952, 1061
HapII CCGG 2 cut(s) 62, 408
HgaI GACGC 1 cut(s) 295
HhaI GCGC 2 cut(s) 492, 517
Hin1I GRCGYC 2 cut(s) 306, 333
Hin1II CATG 5 cut(s) 375, 397, 468, 741, 916
Hin6I GCGC 2 cut(s) 490, 515
HinP1I GCGC 2 cut(s) 490, 515
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 1 cut(s) 1159
HpaII CCGG 2 cut(s) 62, 408
HphI GGTGA 4 cut(s) 208, 463, 889, 1027
Hpy166II GTNNAC 2 cut(s) 232, 1018
Hpy188I TCNGA 7 cut(s) 100, 132, 353, 415, 717, 829, 988
Hpy188III TCNNGA 6 cut(s) 80, 341, 535, 1047, 1175, 1191
Hpy8I GTNNAC 2 cut(s) 232, 1018
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 7 cut(s) 257, 296, 380, 604, 877, 942, 1062
HpyCH4III ACNGT 2 cut(s) 544, 843
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 8 cut(s) 43, 294, 485, 572, 701, 752, 866, 1147
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 417, 815
HpyF3I CTNAG 5 cut(s) 122, 257, 382, 742, 1189
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 2 cut(s) 306, 333
Hsp92II CATG 5 cut(s) 375, 397, 468, 741, 916
HspAI GCGC 2 cut(s) 490, 515
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 3 cut(s) 198, 917, 928
LmnI GCTCC 2 cut(s) 656, 1065
Lsp1109I GCAGC 2 cut(s) 685, 805
LweI GCATC 4 cut(s) 81, 233, 346, 559
MaeI CTAG 2 cut(s) 519, 980
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 55, 334, 788
MalI GATC 3 cut(s) 200, 919, 930
MboI GATC 3 cut(s) 198, 917, 928
MboII GAAGA 6 cut(s) 620, 631, 770, 917, 1122, 1190
MflI RGATCY 1 cut(s) 928
MluCI AATT 1 cut(s) 1117
MnlI CCTC 9 cut(s) 25, 87, 313, 464, 503, 595, 938, 963, 1006
MseI TTAA 1 cut(s) 941
MslI CAYNNNNRTG 1 cut(s) 589
MspA1I CMGCKG 2 cut(s) 698, 818
MspI CCGG 2 cut(s) 62, 408
MspR9I CCNGG 2 cut(s) 408, 1063
Mva1269I GAATGC 2 cut(s) 45, 420
MvaI CCWGG 1 cut(s) 1063
MwoI GCNNNNNNNGC 3 cut(s) 146, 417, 815
NciI CCSGG 1 cut(s) 408
NdeII GATC 3 cut(s) 198, 917, 928
NlaIII CATG 5 cut(s) 375, 397, 468, 741, 916
NmuCI GTSAC 1 cut(s) 334
NspI RCATGY 2 cut(s) 468, 916
OliI CACNNNNGTG 1 cut(s) 589
PaeR7I CTCGAG 2 cut(s) 341, 1173
PceI AGGCCT 4 cut(s) 120, 614, 952, 1061
PciI ACATGT 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 2 cut(s) 45, 420
PfeI GAWTC 1 cut(s) 1159
PflMI CCANNNNNTGG 1 cut(s) 1062
PkrI GCNGC 3 cut(s) 692, 700, 820
PscI ACATGT 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 1061
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 1 cut(s) 1061
PspPI GGNCC 3 cut(s) 266, 526, 1049
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 1 cut(s) 928
PvuII CAGCTG 2 cut(s) 698, 818
RsaI GTAC 2 cut(s) 233, 911
RsaNI GTAC 2 cut(s) 232, 910
RseI CAYNNNNRTG 1 cut(s) 589
SaqAI TTAA 1 cut(s) 941
SatI GCNGC 3 cut(s) 691, 699, 819
Sau3AI GATC 3 cut(s) 198, 917, 928
Sau96I GGNCC 3 cut(s) 266, 526, 1049
ScrFI CCNGG 2 cut(s) 408, 1063
SfaNI GCATC 4 cut(s) 81, 233, 346, 559
Sfr274I CTCGAG 2 cut(s) 341, 1173
SinI GGWCC 2 cut(s) 526, 1049
SlaI CTCGAG 2 cut(s) 341, 1173
SmiMI CAYNNNNRTG 1 cut(s) 589
SmlI CTYRAG 4 cut(s) 341, 558, 704, 1173
SmoI CTYRAG 4 cut(s) 341, 558, 704, 1173
Sse9I AATT 1 cut(s) 1117
SseBI AGGCCT 4 cut(s) 120, 614, 952, 1061
SsiI CCGC 3 cut(s) 632, 691, 926
SspI AATATT 2 cut(s) 24, 1072
SspMI CTAG 2 cut(s) 519, 980
StuI AGGCCT 4 cut(s) 120, 614, 952, 1061
StyD4I CCNGG 2 cut(s) 406, 1061
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 2 cut(s) 544, 843
TaiI ACGT 1 cut(s) 336
TaqI TCGA 8 cut(s) 37, 167, 173, 318, 342, 364, 965, 1174
TasI AATT 1 cut(s) 1117
TatI WGTACW 2 cut(s) 231, 909
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 1 cut(s) 1159
Tru1I TTAA 1 cut(s) 941
Tru9I TTAA 1 cut(s) 941
TscAI CASTG 4 cut(s) 594, 873, 927, 1135
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 2 cut(s) 698, 818
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 7 cut(s) 382, 466, 623, 701, 726, 896, 918
TspRI CASTG 4 cut(s) 594, 873, 927, 1135
Van91I CCANNNNNTGG 1 cut(s) 1062
VpaK11BI GGWCC 2 cut(s) 526, 1049
XapI RAATTY 1 cut(s) 1117
XceI RCATGY 2 cut(s) 468, 916
XhoI CTCGAG 2 cut(s) 341, 1173
XspI CTAG 2 cut(s) 519, 980
ZraI GACGTC 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.