MD00G1170000.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002596
Physical Location & Seq
Reverse (-)
39134227 .. 39135471
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1170000.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTCATCTAGCTTTCGACGGTTGGGTGAGACTGAAAGGCCACTTGCTGTCAGAAAAAGAATGTTTGGTCGTGCCTGGCCTCTTAGAGCTCCTGTGAGGGATGGTATTGTTCCAACTTGGCCGGAGCTGATGGATAGTGAAAGTGTTCCGTGTATTGATTTTTCATGGGAATTATGCACAAGCAGAATTGAATGTTATCACGATTCTGGAAGTATGATCAAGTACCACAAGAACCTCACTGCAAAGGGTTATCGGGCACTTATATATAGCGGTGATCATGATATGTGTGTTCCTTTCACTGGGAGTGAAGCATGGACTAGATCACTTGGTTATAAGATTGTGGATGAATGGAGGCCATGGACTTCGAACGGACAAGTTGCTGGGTACACACAGGGTTACGAGAATAACCTCACCTTTCTAACTGTAAAGGGGTCTGGACATACAGTTCCAGAGTACAAACCACGAGAGGCGTTTGATTTGTTTAGCCGGTTTTTGGCTGGAAAACCACAATAA

Protein Analysis

171

Amino Acids

19.48

Weight (kDa)

8.68

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 55 - 166 1.4e-35 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AciI CCGC 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 119
AfaI GTAC 3 cut(s) 224, 386, 455
AfiI CCNNNNNNNGG 2 cut(s) 299, 493
AgsI TTSAA 1 cut(s) 191
AjnI CCWGG 1 cut(s) 74
AloI GAACNNNNNNTCC 2 cut(s) 429, 461
AluBI AGCT 3 cut(s) 12, 89, 127
AluI AGCT 3 cut(s) 12, 89, 127
Alw21I GWGCWC 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 23
AoxI GGCC 4 cut(s) 38, 77, 119, 353
Asp700I GAANNNNTTC 1 cut(s) 144
AsuHPI GGTGA 3 cut(s) 38, 284, 403
AsuII TTCGAA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 91
BauI CACGAG 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 91
BccI CCATC 2 cut(s) 95, 124
BciT130I CCWGG 1 cut(s) 76
BclI TGATCA 2 cut(s) 216, 274
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 2 cut(s) 9, 318
Bme1390I CCNGG 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 76
BmrI ACTGGG 1 cut(s) 309
BmuI ACTGGG 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 356
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 493
Bse118I RCCGGY 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 106, 349
BseLI CCNNNNNNNGG 2 cut(s) 299, 493
BseNI ACTGG 1 cut(s) 304
BseSI GKGCMC 1 cut(s) 259
BseYI CCCAGC 1 cut(s) 380
BshFI GGCC 4 cut(s) 40, 79, 121, 355
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 2 cut(s) 122, 487
BslI CCNNNNNNNGG 2 cut(s) 299, 493
BsmAI GTCTC 1 cut(s) 23
BsnI GGCC 4 cut(s) 40, 79, 121, 355
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 2 cut(s) 91, 259
Bsp143I GATC 3 cut(s) 216, 274, 320
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 1 cut(s) 270
BspANI GGCC 4 cut(s) 40, 79, 121, 355
BspHI TCATGA 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 365
BsrFI RCCGGY 1 cut(s) 486
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 486
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 3 cut(s) 216, 274, 320
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 356
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 3 cut(s) 21, 424, 445
BstBI TTCGAA 1 cut(s) 365
BstDEI CTNAG 1 cut(s) 83
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 106, 349
BstKTI GATC 3 cut(s) 219, 277, 323
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 3 cut(s) 216, 274, 320
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSLI GKGCMC 1 cut(s) 259
BsuRI GGCC 4 cut(s) 40, 79, 121, 355
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 2 cut(s) 106, 349
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 237, 297
CciI TCATGA 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 486
Csp6I GTAC 3 cut(s) 223, 385, 454
CviAII CATG 4 cut(s) 165, 278, 312, 357
CviJI RGCY 9 cut(s) 12, 40, 79, 89, 121, 127, 355, 486, 497
CviKI_1 RGCY 9 cut(s) 12, 40, 79, 89, 121, 127, 355, 486, 497
CviQI GTAC 3 cut(s) 223, 385, 454
DdeI CTNAG 1 cut(s) 83
DpnI GATC 3 cut(s) 218, 276, 322
DpnII GATC 3 cut(s) 216, 274, 320
EaeI YGGCCR 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 356
Eco24I GRGCYC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 4 cut(s) 168, 281, 315, 360
FatI CATG 4 cut(s) 164, 277, 311, 356
FbaI TGATCA 2 cut(s) 216, 274
FokI GGATG 2 cut(s) 113, 356
FriOI GRGCYC 1 cut(s) 91
FspBI CTAG 2 cut(s) 9, 318
GsaI CCCAGC 1 cut(s) 384
HaeIII GGCC 4 cut(s) 40, 79, 121, 355
HapII CCGG 2 cut(s) 122, 487
Hin1II CATG 4 cut(s) 168, 281, 315, 360
HinfI GANTC 1 cut(s) 203
HpaII CCGG 2 cut(s) 122, 487
HphI GGTGA 3 cut(s) 38, 284, 403
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 5 cut(s) 200, 207, 278, 435, 449
Hpy8I GTNNAC 1 cut(s) 387
Hpy99I CGWCG 1 cut(s) 21
HpyCH4III ACNGT 3 cut(s) 21, 424, 445
HpyCH4V TGCA 2 cut(s) 177, 242
HpyF3I CTNAG 1 cut(s) 83
Hsp92II CATG 4 cut(s) 168, 281, 315, 360
Ksp22I TGATCA 2 cut(s) 216, 274
Kzo9I GATC 3 cut(s) 216, 274, 320
LmnI GCTCC 2 cut(s) 94, 124
MaeI CTAG 2 cut(s) 9, 318
MaeIII GTNAC 1 cut(s) 395
MalI GATC 3 cut(s) 218, 276, 322
MboI GATC 3 cut(s) 216, 274, 320
MhlI GDGCHC 2 cut(s) 91, 259
MluCI AATT 2 cut(s) 170, 186
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 6 cut(s) 90, 90, 245, 345, 419, 460
MroXI GAANNNNTTC 1 cut(s) 144
MspI CCGG 2 cut(s) 122, 487
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
NcoI CCATGG 1 cut(s) 356
NdeII GATC 3 cut(s) 216, 274, 320
NlaIII CATG 4 cut(s) 168, 281, 315, 360
NspV TTCGAA 1 cut(s) 365
PagI TCATGA 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 1 cut(s) 203
PsiI TTATAA 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 380
PspGI CCWGG 1 cut(s) 74
RsaI GTAC 3 cut(s) 224, 386, 455
RsaNI GTAC 3 cut(s) 223, 385, 454
SacI GAGCTC 1 cut(s) 91
Sau3AI GATC 3 cut(s) 216, 274, 320
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 2 cut(s) 91, 259
SetI ASST 6 cut(s) 14, 91, 129, 237, 411, 416
SfuI TTCGAA 1 cut(s) 365
Sse9I AATT 2 cut(s) 170, 186
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 9, 318
SstI GAGCTC 1 cut(s) 91
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 3 cut(s) 21, 424, 445
TaqI TCGA 2 cut(s) 16, 365
TasI AATT 2 cut(s) 170, 186
TatI WGTACW 1 cut(s) 453
TfiI GAWTC 1 cut(s) 203
TscAI CASTG 2 cut(s) 244, 304
TspDTI ATGAA 2 cut(s) 153, 360
TspGWI ACGGA 2 cut(s) 138, 384
TspRI CASTG 2 cut(s) 244, 304
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 2 cut(s) 9, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.