Rh3CG005000

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002596
Physical Location & Seq
Forward (+)
373189 .. 376908
3720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG005000.1

Sequence Viewer

Length: 1257 bp
ATGCGCAGCATTAATATAAAACCTCACTTGCTTGGCTTCATTTGCTTCTCAATTCTCATTCTCATTGGTTCTCCCAACCAACCGCCCACTCTCTCTCTCTCCCCTCACACAAACCTCGTTTCTCAAGCACCTCCAGCCATGGCAGGCATGGCCAGACCGTTGGCTACAATTCCCACCTGCTTCTCAATCATAATCATATGCTTAAGCTTGATAACAATGAGAATTGAGGCAGCCCCTCAAGGCTCTCTTGTCACCCAGCTTCCCGGCTTCAATGGCACTTTTCCGTCCAGCCACTATTCCGGATATGTAAGCGTAGGCGCAAAGAATCTTTTCTACTACTTCGTAGTGTCAGAAGGGAGCCCCGCAGACGATCCTGTTGTCTTGTGGCTCAATGGCGGACCCGGCTGCTCTAGCTTTGACGGATTCGTCTACGAACACGGGCCGTTCAATTTTGAACAAAAATCGAAAGGAGCATTGCCCACTTTACAACTTAATCCTTACAGCTGGTCCAAGGTTTCCAACATGCTGTATTTGGATTCTCCTGCTGGTGTTGGGTTTTCCTACTCAGAAGACCCAAGCCAATACGTAACCGGGGATCTTCAAACTGCTGCCGACACCCACGCCTTCCTCCTCAGCTGGTTTAAGCAGTACCCGGAGTTTCTTTCCAATCCCTTTTACATTTCCGGGGAATCTTATGCTGGAGTCTACGTCCCTACTCTTGCCTTTCAAGTTGCCACAGGTATTCAAAATGGAACAAAACCAGTTGTAAATCTCAAGGGTTACATGGTGGGGAATGCAGTCACAGACAGAAACTTTGATGACAATGCTCTTGTCCCTTTTGCTCATGGGATGGCCCTCATTTCAGATCAAATTTATGAGGGAGTTATAGAAGAATGTGGGATCAACTACTCCAACTCATCTAGTCCCAAGTGCAATGAGAAACTTAATCTGGCTCGCGAGGCTCTTGGTGGACTAAATATCTACAACGTCCTTGAGCCTTGTTACCACAACCCTGGAACTGAATCAGGAAATGATACTTCTACAACAACCAAAAGCAGCAACTTACCCCTTAGCTTTCAGCAACTAGGCAAGTCGCCTGAGAAGCCTCTCGGAGTGAGAAAGAGGATGTTTGGCCGCGCTTGGCCTTATCAGATGTCTGTAGAAGATGGCTTAGTCCCTTTGTGGCCACAGCTAGCTGCTAAATCTAACTCACGCACTGTTCCATGCTTTGTAAGTAATTCACACTTCTATGGTTGA

Protein Analysis

418

Amino Acids

45.42

Weight (kDa)

5.9

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 88 - 353 5.9e-90 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 185
AasI GACNNNNNNGTC 1 cut(s) 425
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 185
AccI GTMKAC 2 cut(s) 429, 705
AccII CGCG 2 cut(s) 957, 1137
AccIII TCCGGA 1 cut(s) 299
AciI CCGC 4 cut(s) 83, 363, 396, 1135
AclWI GGATC 3 cut(s) 365, 603, 908
AcoI YGGCCR 3 cut(s) 150, 1132, 1184
AcsI RAATTY 1 cut(s) 870
AfaI GTAC 1 cut(s) 650
AflII CTTAAG 1 cut(s) 202
AgsI TTSAA 6 cut(s) 271, 448, 455, 602, 728, 746
AjnI CCWGG 1 cut(s) 1012
AluBI AGCT 8 cut(s) 207, 259, 414, 504, 636, 1074, 1192, 1196
AluI AGCT 8 cut(s) 207, 259, 414, 504, 636, 1074, 1192, 1196
AlwI GGATC 3 cut(s) 365, 603, 908
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 6 cut(s) 150, 440, 852, 1132, 1142, 1184
ApeKI GCWGC 6 cut(s) 6, 230, 405, 608, 1056, 1196
ApoI RAATTY 1 cut(s) 870
ArsI GACNNNNNNTTYG 2 cut(s) 797, 829
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 3 cut(s) 6, 320, 1139
AspS9I GGNCC 4 cut(s) 398, 440, 507, 853
AsuC2I CCSGG 5 cut(s) 264, 402, 592, 653, 685
AsuHPI GGTGA 1 cut(s) 244
AsuNHI GCTAGC 1 cut(s) 1192
AvaII GGWCC 2 cut(s) 398, 507
BalI TGGCCA 2 cut(s) 152, 1186
BanII GRGCYC 1 cut(s) 362
BbsI GAAGAC 1 cut(s) 576
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 6 cut(s) 18, 242, 392, 595, 1068, 1183
BccI CCATC 2 cut(s) 844, 1160
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 1 cut(s) 1014
BcnI CCSGG 5 cut(s) 264, 402, 592, 653, 685
BfaI CTAG 4 cut(s) 411, 921, 1085, 1193
BfmI CTRYAG 1 cut(s) 1158
BfrI CTTAAG 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 7 cut(s) 7, 231, 406, 609, 1057, 1135, 1197
BlsI GCNGC 7 cut(s) 8, 232, 407, 610, 1058, 1136, 1198
Bme1390I CCNGG 6 cut(s) 264, 402, 592, 653, 685, 1014
Bme18I GGWCC 2 cut(s) 398, 507
BmgT120I GGNCC 4 cut(s) 398, 440, 507, 853
BmiI GGNNCC 2 cut(s) 359, 400
BmrFI CCNGG 6 cut(s) 264, 402, 592, 653, 685, 1014
BmtI GCTAGC 1 cut(s) 1196
BpiI GAAGAC 1 cut(s) 576
BpmI CTGGAG 2 cut(s) 117, 720
Bpu10I CCTNAGC 2 cut(s) 632, 1070
BpuEI CTTGAG 4 cut(s) 108, 222, 758, 1013
BpuMI CCSGG 5 cut(s) 264, 402, 592, 653, 685
BsaAI YACGTR 1 cut(s) 586
BsaJI CCNNGG 5 cut(s) 138, 510, 591, 684, 1012
BsaWI WCCGGW 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bse1I ACTGG 1 cut(s) 761
Bse3DI GCAATG 2 cut(s) 473, 940
BseAI TCCGGA 1 cut(s) 299
BseBI CCWGG 1 cut(s) 1014
BseDI CCNNGG 5 cut(s) 138, 510, 591, 684, 1012
BseGI GGATG 2 cut(s) 855, 1131
BseMI GCAATG 2 cut(s) 473, 940
BseMII CTCAG 3 cut(s) 579, 646, 1089
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 6 cut(s) 18, 242, 392, 595, 1068, 1183
BseYI CCCAGC 1 cut(s) 255
Bsh1236I CGCG 2 cut(s) 957, 1137
BshFI GGCC 6 cut(s) 152, 442, 854, 1134, 1144, 1186
BsiSI CCGG 6 cut(s) 264, 300, 402, 591, 653, 684
BslFI GGGAC 4 cut(s) 695, 818, 909, 1160
BsmFI GGGAC 4 cut(s) 695, 818, 909, 1160
BsmI GAATGC 1 cut(s) 799
BsnI GGCC 6 cut(s) 152, 442, 854, 1134, 1144, 1186
Bsp1286I GDGCHC 1 cut(s) 362
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 4 cut(s) 370, 595, 865, 900
Bsp19I CCATGG 1 cut(s) 138
Bsp68I TCGCGA 1 cut(s) 957
BspACI CCGC 4 cut(s) 83, 363, 396, 1135
BspANI GGCC 6 cut(s) 152, 442, 854, 1134, 1144, 1186
BspCNI CTCAG 3 cut(s) 578, 645, 1090
BspEI TCCGGA 1 cut(s) 299
BspFNI CGCG 2 cut(s) 957, 1137
BspLI GGNNCC 2 cut(s) 359, 400
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 1196
BspPI GGATC 3 cut(s) 365, 603, 908
BspTI CTTAAG 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 473, 940
BsrI ACTGG 1 cut(s) 761
BssECI CCNNGG 5 cut(s) 138, 510, 591, 684, 1012
BssMI GATC 4 cut(s) 370, 595, 865, 900
BssT1I CCWWGG 2 cut(s) 138, 510
Bst2UI CCWGG 1 cut(s) 1014
Bst4CI ACNGT 2 cut(s) 159, 1219
BstAFI CTTAAG 1 cut(s) 202
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 3 cut(s) 145, 955, 1194
BstDEI CTNAG 5 cut(s) 565, 632, 1070, 1098, 1171
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 855, 1131
BstFNI CGCG 2 cut(s) 957, 1137
BstHHI GCGC 3 cut(s) 6, 320, 1139
BstKTI GATC 4 cut(s) 373, 598, 868, 903
BstMBI GATC 4 cut(s) 370, 595, 865, 900
BstMWI GCNNNNNNNGC 8 cut(s) 42, 134, 149, 273, 402, 411, 959, 1102
BstNI CCWGG 1 cut(s) 1014
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 6 cut(s) 262, 400, 590, 651, 683, 1012
BstSFI CTRYAG 1 cut(s) 1158
BstSNI TACGTA 1 cut(s) 586
BstUI CGCG 2 cut(s) 957, 1137
BstV1I GCAGC 6 cut(s) 18, 242, 392, 595, 1068, 1183
BstV2I GAAGAC 1 cut(s) 576
BstX2I RGATCY 1 cut(s) 595
BstXI CCANNNNNNTGG 2 cut(s) 160, 1013
BstYI RGATCY 1 cut(s) 595
BsuRI GGCC 6 cut(s) 152, 442, 854, 1134, 1144, 1186
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 855, 1131
BtsIMutI CAGTG 1 cut(s) 1215
BtuMI TCGCGA 1 cut(s) 957
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 145, 955, 1194
CfoI GCGC 3 cut(s) 6, 320, 1139
Cfr13I GGNCC 4 cut(s) 398, 440, 507, 853
Csp6I GTAC 1 cut(s) 649
CviAII CATG 6 cut(s) 139, 148, 523, 784, 845, 1224
CviQI GTAC 1 cut(s) 649
DdeI CTNAG 5 cut(s) 565, 632, 1070, 1098, 1171
DpnI GATC 4 cut(s) 372, 597, 867, 902
DpnII GATC 4 cut(s) 370, 595, 865, 900
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 3 cut(s) 150, 1132, 1184
EciI GGCGGA 1 cut(s) 411
Eco105I TACGTA 1 cut(s) 586
Eco130I CCWWGG 2 cut(s) 138, 510
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 2 cut(s) 398, 507
EcoRII CCWGG 1 cut(s) 1012
EcoT14I CCWWGG 2 cut(s) 138, 510
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 2 cut(s) 138, 510
FaeI CATG 6 cut(s) 142, 151, 526, 787, 848, 1227
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 4 cut(s) 695, 818, 909, 1160
FatI CATG 6 cut(s) 138, 147, 522, 783, 844, 1223
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 197
FblI GTMKAC 2 cut(s) 429, 705
Fnu4HI GCNGC 7 cut(s) 7, 231, 406, 609, 1057, 1135, 1197
FokI GGATG 2 cut(s) 862, 1138
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 7 cut(s) 7, 231, 406, 609, 1057, 1135, 1197
FspBI CTAG 4 cut(s) 411, 921, 1085, 1193
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 319, 1138
GluI GCNGC 7 cut(s) 7, 231, 406, 609, 1057, 1135, 1197
GsaI CCCAGC 1 cut(s) 259
GsuI CTGGAG 2 cut(s) 117, 720
HaeIII GGCC 6 cut(s) 152, 442, 854, 1134, 1144, 1186
HapII CCGG 6 cut(s) 264, 300, 402, 591, 653, 684
HhaI GCGC 3 cut(s) 6, 320, 1139
Hin1II CATG 6 cut(s) 142, 151, 526, 787, 848, 1227
Hin6I GCGC 3 cut(s) 4, 318, 1137
HinP1I GCGC 3 cut(s) 4, 318, 1137
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 6 cut(s) 325, 423, 536, 689, 702, 1022
HpaII CCGG 6 cut(s) 264, 300, 402, 591, 653, 684
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 3 cut(s) 430, 706, 971
Hpy188I TCNGA 5 cut(s) 352, 568, 865, 1112, 1152
Hpy188III TCNNGA 3 cut(s) 300, 956, 1026
Hpy8I GTNNAC 3 cut(s) 430, 706, 971
HpyAV CCTTC 2 cut(s) 347, 634
HpyCH4III ACNGT 2 cut(s) 159, 1219
HpyCH4IV ACGT 3 cut(s) 585, 708, 987
HpyCH4V TGCA 2 cut(s) 797, 933
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 134, 149, 273, 402, 411, 959, 1102
HpyF3I CTNAG 5 cut(s) 565, 632, 1070, 1098, 1171
HpySE526I ACGT 3 cut(s) 585, 708, 987
Hsp92II CATG 6 cut(s) 142, 151, 526, 787, 848, 1227
HspAI GCGC 3 cut(s) 4, 318, 1137
Kpn2I TCCGGA 1 cut(s) 299
Kzo9I GATC 4 cut(s) 370, 595, 865, 900
LmnI GCTCC 2 cut(s) 357, 470
Lsp1109I GCAGC 6 cut(s) 18, 242, 392, 595, 1068, 1183
MaeI CTAG 4 cut(s) 411, 921, 1085, 1193
MaeII ACGT 3 cut(s) 585, 708, 987
MaeIII GTNAC 5 cut(s) 250, 586, 779, 799, 1001
MalI GATC 4 cut(s) 372, 597, 867, 902
MboI GATC 4 cut(s) 370, 595, 865, 900
MboII GAAGA 4 cut(s) 581, 590, 902, 1175
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 362
MlsI TGGCCA 2 cut(s) 152, 1186
MluCI AATT 6 cut(s) 51, 168, 222, 448, 870, 1237
MluNI TGGCCA 2 cut(s) 152, 1186
MlyI GAGTC 1 cut(s) 711
MmeI TCCRAC 2 cut(s) 543, 936
Mox20I TGGCCA 2 cut(s) 152, 1186
MroI TCCGGA 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 329
MscI TGGCCA 2 cut(s) 152, 1186
MseI TTAA 5 cut(s) 12, 203, 492, 642, 945
MslI CAYNNNNRTG 1 cut(s) 1248
Msp20I TGGCCA 2 cut(s) 152, 1186
MspA1I CMGCKG 2 cut(s) 504, 636
MspCI CTTAAG 1 cut(s) 202
MspI CCGG 6 cut(s) 264, 300, 402, 591, 653, 684
MspR9I CCNGG 6 cut(s) 264, 402, 592, 653, 685, 1014
Mva1269I GAATGC 1 cut(s) 799
MvaI CCWGG 1 cut(s) 1014
MvnI CGCG 2 cut(s) 957, 1137
MwoI GCNNNNNNNGC 8 cut(s) 42, 134, 149, 273, 402, 411, 959, 1102
NciI CCSGG 5 cut(s) 264, 402, 592, 653, 685
NcoI CCATGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 197
NdeII GATC 4 cut(s) 370, 595, 865, 900
NheI GCTAGC 1 cut(s) 1192
NlaIII CATG 6 cut(s) 142, 151, 526, 787, 848, 1227
NlaIV GGNNCC 2 cut(s) 359, 400
NmuCI GTSAC 2 cut(s) 250, 799
NruI TCGCGA 1 cut(s) 957
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 1 cut(s) 526
PaqCI CACCTGC 1 cut(s) 185
PctI GAATGC 1 cut(s) 799
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 5 cut(s) 325, 423, 536, 689, 1022
PkrI GCNGC 7 cut(s) 8, 232, 407, 610, 1058, 1136, 1198
PleI GAGTC 1 cut(s) 710
PpsI GAGTC 1 cut(s) 710
Ppu21I YACGTR 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 1012
PspFI CCCAGC 1 cut(s) 255
PspGI CCWGG 1 cut(s) 1012
PspN4I GGNNCC 2 cut(s) 359, 400
PspPI GGNCC 4 cut(s) 398, 440, 507, 853
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 504, 636
RruI TCGCGA 1 cut(s) 957
RsaI GTAC 1 cut(s) 650
RsaNI GTAC 1 cut(s) 649
RseI CAYNNNNRTG 1 cut(s) 1248
SaqAI TTAA 5 cut(s) 12, 203, 492, 642, 945
SatI GCNGC 7 cut(s) 7, 231, 406, 609, 1057, 1135, 1197
Sau3AI GATC 4 cut(s) 370, 595, 865, 900
Sau96I GGNCC 4 cut(s) 398, 440, 507, 853
SchI GAGTC 1 cut(s) 711
ScrFI CCNGG 6 cut(s) 264, 402, 592, 653, 685, 1014
SduI GDGCHC 1 cut(s) 362
SfcI CTRYAG 1 cut(s) 1158
SinI GGWCC 2 cut(s) 398, 507
SmiMI CAYNNNNRTG 1 cut(s) 1248
SmlI CTYRAG 5 cut(s) 123, 202, 237, 773, 992
SmoI CTYRAG 5 cut(s) 123, 202, 237, 773, 992
SnaBI TACGTA 1 cut(s) 586
Sse9I AATT 6 cut(s) 51, 168, 222, 448, 870, 1237
SsiI CCGC 4 cut(s) 83, 363, 396, 1135
SspMI CTAG 4 cut(s) 411, 921, 1085, 1193
StyD4I CCNGG 6 cut(s) 262, 400, 590, 651, 683, 1012
StyI CCWWGG 2 cut(s) 138, 510
TaaI ACNGT 2 cut(s) 159, 1219
TaiI ACGT 3 cut(s) 588, 711, 990
TaqI TCGA 1 cut(s) 464
TasI AATT 6 cut(s) 51, 168, 222, 448, 870, 1237
TauI GCSGC 1 cut(s) 1137
TfiI GAWTC 5 cut(s) 325, 423, 536, 689, 1022
Tru1I TTAA 5 cut(s) 12, 203, 492, 642, 945
Tru9I TTAA 5 cut(s) 12, 203, 492, 642, 945
TscAI CASTG 1 cut(s) 1222
TseFI GTSAC 2 cut(s) 250, 799
TseI GCWGC 6 cut(s) 6, 230, 405, 608, 1056, 1196
Tsp45I GTSAC 2 cut(s) 250, 799
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 2 cut(s) 273, 435
TspRI CASTG 1 cut(s) 1222
Vha464I CTTAAG 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 398, 507
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 1 cut(s) 870
XceI RCATGY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmiI GTMKAC 2 cut(s) 429, 705
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 4 cut(s) 411, 921, 1085, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.