Rw6G022640

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002596
Physical Location & Seq
Reverse (-)
44586038 .. 44590562
4525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022640.1

Sequence Viewer

Length: 1500 bp
ATGCAACTCGGTTCTTCTTCCCAAGTTGAGAGCTCAGTTTATCTCTTGGCTTGGAAGCAACGAAATTCAGCAACTTGCAGATCAGCTCCTGAGGCAGCACTCATAACCCAGATTCCTGGATTCAATGGCACTCTTCCTTCAAAGCACCGTTCTGGGTACGTGACAATTGATGAGAGTCATGGGAGGAACCTGTTTTACTATTTTGTGGAATCGGAGAGGAAGCCGTCGGAGGATCCGGTTGTGCTGTGGCTGAATGGTGGACCTGGTTGCTCCAGTTTTGATGGCTTTGTTTATGAGCATGGGCCATTCAATTTTGAAGCAGCAAACACCAAGGGAGGTCTGCCCAAATTGCACCTTAATCCATACAGCTGGTCAAAGGTTTCCAACATTTTATATTTGGATTCTCCTGCTGGTGTTGGGCTGTCTTACTCCAAAAACAAAAGTGACTATATTACTGGTGATCTAAAGACTGCATCTGATAGCCACACATTTCTCCTCAAGTGGTTTGAACTTTACCCAGAGTTCCTCTCCAACCCGTTTTTCATTTCGGGAGAGTCATATGCTGGTGTCTACGTGCCAACACTTGCTTTTGAAGTGGCCAAAGGAATTGATGCTGGTACAAAGCCCATTATGAACTTCAAGGGGTATCTTGTGGGAAATGGAGTCACAGATGAGGAAATCGATGGGAATGCCCTTGTTCCATTTGCTCATGGGATGGGACTTATATCAGATGATTTATTTGAGGAGGTTATCAAGGAGTGCAATGGAAACTACTATCGGCCGAATAGTGATACTTGTGCGAACAACCTTGAAAAAGTTGACAACGCCATAGAAGGCTTGAACATTTATGACATTCTAGAACCATGCTATCACAGCACAGATTCTAAGAAAATTTCAACCGTAACCAGTAACTTACCATCTAGCTTTCGAAAGTTGGGTGAAACTGAAAGGCCTCTTGCTGTGAGAAAAAGGATGTTTGGTCGCGCATGGCCTCTTAGAGCTCCTGTGAGAGCTGGACTTGTTCCAACTTGGCCGCAGCTTATGAATAGTGAGGAAGTTCCATGCACTGATGATGAGGTTGCAACTGCATGGTTGAACAATGCAGCAGTTAGGAAGGCAATCCATGCAGAAGATGAAAGCGTGATTCCTAGTTGGGAATTATGTACAGACAACATTCGTTTTTACCATGATGCTGGGAGTATGATCCAGTACCACAAGAATCTGACTTCGAAGGGATACCGTGCGCTTATATATAGTGGTGATCATGATCTGTGTGTTCCATTCACCGGGAGTGAAGCATGGACTAGATCAGTTGGTTACAAGATTGTGGATGAATGGAGGCCATGGATTTCCCAGGGACAAATTGCTGGGTATACACAAGGGTATGAAAACAACCTCACGTTTCTAACCATAAAGGGAGCTGGTCATACGGTTCCAGAATACAAACCCCAAGAGGCATTAGATTTGTATAGCCGATTCTTGGCTGGACAACCACTCTAG

Protein Analysis

499

Amino Acids

55.52

Weight (kDa)

5.39

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 39 - 495 9.6e-143 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 570, 1373
AccII CGCG 1 cut(s) 984
AciI CCGC 1 cut(s) 1034
AclWI GGATC 3 cut(s) 227, 240, 1198
AcoI YGGCCR 3 cut(s) 597, 779, 1031
AcsI RAATTY 2 cut(s) 64, 891
AfaI GTAC 4 cut(s) 158, 619, 1165, 1211
AfiI CCNNNNNNNGG 2 cut(s) 1286, 1480
AjnI CCWGG 3 cut(s) 115, 262, 1353
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AluBI AGCT 8 cut(s) 33, 86, 369, 924, 1001, 1013, 1039, 1421
AluI AGCT 8 cut(s) 33, 86, 369, 924, 1001, 1013, 1039, 1421
Alw21I GWGCWC 2 cut(s) 35, 1003
AlwI GGATC 3 cut(s) 227, 240, 1198
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 7 cut(s) 302, 597, 779, 950, 989, 1031, 1340
ApeKI GCWGC 4 cut(s) 95, 320, 1036, 1103
ApoI RAATTY 2 cut(s) 64, 891
AspLEI GCGC 2 cut(s) 986, 1246
AspS9I GGNCC 2 cut(s) 260, 302
AsuC2I CCSGG 1 cut(s) 1288
AsuHPI GGTGA 4 cut(s) 470, 950, 1271, 1276
AsuII TTCGAA 2 cut(s) 928, 1229
AvaII GGWCC 1 cut(s) 260
AxyI CCTNAGG 1 cut(s) 90
BalI TGGCCA 1 cut(s) 599
BamHI GGATCC 1 cut(s) 232
BanII GRGCYC 2 cut(s) 35, 1003
Bbv12I GWGCWC 2 cut(s) 35, 1003
BbvI GCAGC 4 cut(s) 107, 332, 1048, 1115
BccI CCATC 4 cut(s) 275, 677, 709, 925
BceAI ACGGC 1 cut(s) 208
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 3 cut(s) 117, 264, 1355
BciVI GTATCC 1 cut(s) 1229
BclI TGATCA 1 cut(s) 1261
BcnI CCSGG 1 cut(s) 1288
BfaI CTAG 5 cut(s) 857, 921, 1149, 1305, 1498
BfuI GTATCC 1 cut(s) 1229
BisI GCNGC 5 cut(s) 96, 321, 1034, 1037, 1104
BlsI GCNGC 5 cut(s) 97, 322, 1035, 1038, 1105
Bme1390I CCNGG 4 cut(s) 117, 264, 1288, 1355
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 260, 302
BmiI GGNNCC 3 cut(s) 188, 234, 1434
BmrFI CCNGG 4 cut(s) 117, 264, 1288, 1355
BmsI GCATC 3 cut(s) 482, 601, 1180
BplI GAGNNNNNCTC 2 cut(s) 512, 544
BpmI CTGGAG 1 cut(s) 256
Bpu14I TTCGAA 2 cut(s) 928, 1229
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 1288
Bsa29I ATCGAT 1 cut(s) 681
BsaAI YACGTR 2 cut(s) 160, 574
BsaBI GATNNNNATC 1 cut(s) 1266
BsaJI CCNNGG 4 cut(s) 330, 1343, 1353, 1354
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 2 cut(s) 1286, 1480
Bse1I ACTGG 4 cut(s) 273, 460, 906, 1207
Bse21I CCTNAGG 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 769
Bse8I GATNNNNATC 1 cut(s) 1266
BseBI CCWGG 3 cut(s) 117, 264, 1355
BseCI ATCGAT 1 cut(s) 681
BseDI CCNNGG 4 cut(s) 330, 1343, 1353, 1354
BseGI GGATG 3 cut(s) 720, 978, 1336
BseJI GATNNNNATC 1 cut(s) 1266
BseLI CCNNNNNNNGG 2 cut(s) 1286, 1480
BseMI GCAATG 1 cut(s) 769
BseMII CTCAG 2 cut(s) 48, 81
BseNI ACTGG 4 cut(s) 273, 460, 906, 1207
BseRI GAGGAG 2 cut(s) 485, 758
BseX3I CGGCCG 1 cut(s) 779
BseXI GCAGC 4 cut(s) 107, 332, 1048, 1115
BseYI CCCAGC 2 cut(s) 1193, 1367
Bsh1236I CGCG 1 cut(s) 984
Bsh1285I CGRYCG 1 cut(s) 782
BshFI GGCC 7 cut(s) 304, 599, 781, 952, 991, 1033, 1342
BshVI ATCGAT 1 cut(s) 681
BsiEI CGRYCG 1 cut(s) 782
BsiHKAI GWGCWC 2 cut(s) 35, 1003
BsiSI CCGG 2 cut(s) 236, 1287
BslFI GGGAC 2 cut(s) 732, 1371
BslI CCNNNNNNNGG 2 cut(s) 1286, 1480
BsmFI GGGAC 2 cut(s) 732, 1371
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 7 cut(s) 304, 599, 781, 952, 991, 1033, 1342
Bsp119I TTCGAA 2 cut(s) 928, 1229
Bsp1286I GDGCHC 2 cut(s) 35, 1003
Bsp1407I TGTACA 1 cut(s) 1163
Bsp143I GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
Bsp19I CCATGG 1 cut(s) 1343
BspACI CCGC 1 cut(s) 1034
BspANI GGCC 7 cut(s) 304, 599, 781, 952, 991, 1033, 1342
BspCNI CTCAG 2 cut(s) 47, 82
BspDI ATCGAT 1 cut(s) 681
BspFNI CGCG 1 cut(s) 984
BspHI TCATGA 1 cut(s) 1264
BspLI GGNNCC 3 cut(s) 188, 234, 1434
BspPI GGATC 3 cut(s) 227, 240, 1198
BspT104I TTCGAA 2 cut(s) 928, 1229
BsrDI GCAATG 1 cut(s) 769
BsrGI TGTACA 1 cut(s) 1163
BsrI ACTGG 4 cut(s) 273, 460, 906, 1207
BssECI CCNNGG 4 cut(s) 330, 1343, 1353, 1354
BssMI GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
BssNAI GTATAC 1 cut(s) 1374
BssT1I CCWWGG 2 cut(s) 330, 1343
Bst1107I GTATAC 1 cut(s) 1374
Bst2UI CCWGG 3 cut(s) 117, 264, 1355
Bst4CI ACNGT 4 cut(s) 149, 901, 1241, 1432
Bst6I CTCTTC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 1124
BstAUI TGTACA 1 cut(s) 1163
BstBAI YACGTR 2 cut(s) 160, 574
BstBI TTCGAA 2 cut(s) 928, 1229
BstDEI CTNAG 4 cut(s) 34, 90, 885, 995
BstDSI CCRYGG 1 cut(s) 1343
BstF5I GGATG 3 cut(s) 720, 978, 1336
BstFNI CGCG 1 cut(s) 984
BstHHI GCGC 2 cut(s) 986, 1246
BstKTI GATC 7 cut(s) 83, 235, 463, 1206, 1264, 1270, 1310
BstMBI GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
BstMCI CGRYCG 1 cut(s) 782
BstMWI GCNNNNNNNGC 4 cut(s) 92, 349, 873, 1124
BstNI CCWGG 3 cut(s) 117, 264, 1355
BstSCI CCNGG 4 cut(s) 115, 262, 1286, 1353
BstUI CGCG 1 cut(s) 984
BstV1I GCAGC 4 cut(s) 107, 332, 1048, 1115
BstX2I RGATCY 1 cut(s) 232
BstXI CCANNNNNNTGG 3 cut(s) 116, 369, 1193
BstYI RGATCY 1 cut(s) 232
BstZ17I GTATAC 1 cut(s) 1374
BstZI CGGCCG 1 cut(s) 779
Bsu15I ATCGAT 1 cut(s) 681
Bsu36I CCTNAGG 1 cut(s) 90
BsuI GTATCC 1 cut(s) 1229
BsuRI GGCC 7 cut(s) 304, 599, 781, 952, 991, 1033, 1342
BsuTUI ATCGAT 1 cut(s) 681
BtgI CCRYGG 1 cut(s) 1343
BtsCI GGATG 3 cut(s) 720, 978, 1336
BtsIMutI CAGTG 1 cut(s) 1065
CaiI CAGNNNCTG 1 cut(s) 89
CciI TCATGA 1 cut(s) 1264
CfoI GCGC 2 cut(s) 986, 1246
Cfr13I GGNCC 2 cut(s) 260, 302
ClaI ATCGAT 1 cut(s) 681
CsiI ACCWGGT 1 cut(s) 262
Csp6I GTAC 4 cut(s) 157, 618, 1164, 1210
CviQI GTAC 4 cut(s) 157, 618, 1164, 1210
DdeI CTNAG 4 cut(s) 34, 90, 885, 995
DpnI GATC 7 cut(s) 82, 234, 462, 1205, 1263, 1269, 1309
DpnII GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
EaeI YGGCCR 3 cut(s) 597, 779, 1031
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Ecl136II GAGCTC 2 cut(s) 33, 1001
EclXI CGGCCG 1 cut(s) 779
Eco130I CCWWGG 2 cut(s) 330, 1343
Eco147I AGGCCT 1 cut(s) 952
Eco24I GRGCYC 2 cut(s) 35, 1003
Eco47I GGWCC 1 cut(s) 260
Eco52I CGGCCG 1 cut(s) 779
Eco53kI GAGCTC 2 cut(s) 33, 1001
Eco81I CCTNAGG 1 cut(s) 90
EcoICRI GAGCTC 2 cut(s) 33, 1001
EcoRII CCWGG 3 cut(s) 115, 262, 1353
EcoT14I CCWWGG 2 cut(s) 330, 1343
EcoT38I GRGCYC 2 cut(s) 35, 1003
ErhI CCWWGG 2 cut(s) 330, 1343
FaqI GGGAC 2 cut(s) 732, 1371
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 1 cut(s) 1261
FblI GTMKAC 2 cut(s) 570, 1373
Fnu4HI GCNGC 5 cut(s) 96, 321, 1034, 1037, 1104
FokI GGATG 3 cut(s) 727, 985, 1343
FriOI GRGCYC 2 cut(s) 35, 1003
Fsp4HI GCNGC 5 cut(s) 96, 321, 1034, 1037, 1104
FspBI CTAG 5 cut(s) 857, 921, 1149, 1305, 1498
GlaI GCGC 2 cut(s) 985, 1245
GluI GCNGC 5 cut(s) 96, 321, 1034, 1037, 1104
GsaI CCCAGC 2 cut(s) 1197, 1371
GsuI CTGGAG 1 cut(s) 256
HaeIII GGCC 7 cut(s) 304, 599, 781, 952, 991, 1033, 1342
HapII CCGG 2 cut(s) 236, 1287
HhaI GCGC 2 cut(s) 986, 1246
Hin6I GCGC 2 cut(s) 984, 1244
HinP1I GCGC 2 cut(s) 984, 1244
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HpaII CCGG 2 cut(s) 236, 1287
HphI GGTGA 4 cut(s) 470, 950, 1271, 1276
Hpy166II GTNNAC 4 cut(s) 260, 571, 820, 1374
Hpy188I TCNGA 5 cut(s) 214, 229, 478, 730, 1224
Hpy188III TCNNGA 5 cut(s) 89, 549, 857, 1265, 1436
Hpy8I GTNNAC 4 cut(s) 260, 571, 820, 1374
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 4 cut(s) 147, 827, 1108, 1225
HpyCH4III ACNGT 4 cut(s) 149, 901, 1241, 1432
HpyCH4IV ACGT 3 cut(s) 159, 573, 1400
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 349, 873, 1124
HpyF3I CTNAG 4 cut(s) 34, 90, 885, 995
HpySE526I ACGT 3 cut(s) 159, 573, 1400
HspAI GCGC 2 cut(s) 984, 1244
Ksp22I TGATCA 1 cut(s) 1261
Kzo9I GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
LmnI GCTCC 4 cut(s) 91, 275, 1006, 1418
Lsp1109I GCAGC 4 cut(s) 107, 332, 1048, 1115
LweI GCATC 3 cut(s) 482, 601, 1180
MabI ACCWGGT 1 cut(s) 262
MaeI CTAG 5 cut(s) 857, 921, 1149, 1305, 1498
MaeII ACGT 3 cut(s) 159, 573, 1400
MaeIII GTNAC 6 cut(s) 160, 443, 664, 901, 908, 1316
MalI GATC 7 cut(s) 82, 234, 462, 1205, 1263, 1269, 1309
MboI GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
MboII GAAGA 4 cut(s) 6, 9, 125, 1142
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 35, 1003
MlsI TGGCCA 1 cut(s) 599
MluCI AATT 8 cut(s) 64, 165, 310, 347, 606, 891, 1157, 1362
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 3 cut(s) 184, 563, 672
MmeI TCCRAC 4 cut(s) 207, 408, 555, 1049
Mox20I TGGCCA 1 cut(s) 599
MscI TGGCCA 1 cut(s) 599
MseI TTAA 1 cut(s) 357
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 2 cut(s) 236, 1287
MspR9I CCNGG 4 cut(s) 117, 264, 1288, 1355
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 3 cut(s) 117, 264, 1355
MvnI CGCG 1 cut(s) 984
MwoI GCNNNNNNNGC 4 cut(s) 92, 349, 873, 1124
NciI CCSGG 1 cut(s) 1288
NcoI CCATGG 1 cut(s) 1343
NdeI CATATG 1 cut(s) 559
NdeII GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
NlaIV GGNNCC 3 cut(s) 188, 234, 1434
NmuCI GTSAC 3 cut(s) 160, 443, 664
NspV TTCGAA 2 cut(s) 928, 1229
PagI TCATGA 1 cut(s) 1264
PasI CCCWGGG 1 cut(s) 1354
PceI AGGCCT 1 cut(s) 952
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 8 cut(s) 112, 120, 209, 401, 881, 1144, 1219, 1476
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 5 cut(s) 97, 322, 1035, 1038, 1105
PleI GAGTC 3 cut(s) 183, 562, 671
PpsI GAGTC 3 cut(s) 183, 562, 671
Ppu21I YACGTR 2 cut(s) 160, 574
Psp124BI GAGCTC 2 cut(s) 35, 1003
Psp6I CCWGG 3 cut(s) 115, 262, 1353
PspFI CCCAGC 2 cut(s) 1193, 1367
PspGI CCWGG 3 cut(s) 115, 262, 1353
PspN4I GGNNCC 3 cut(s) 188, 234, 1434
PspPI GGNCC 2 cut(s) 260, 302
PsrI GAACNNNNNNTAC 2 cut(s) 179, 211
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 4 cut(s) 158, 619, 1165, 1211
RsaNI GTAC 4 cut(s) 157, 618, 1164, 1210
SacI GAGCTC 2 cut(s) 35, 1003
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 5 cut(s) 96, 321, 1034, 1037, 1104
Sau3AI GATC 7 cut(s) 80, 232, 460, 1203, 1261, 1267, 1307
Sau96I GGNCC 2 cut(s) 260, 302
SchI GAGTC 3 cut(s) 184, 563, 672
ScrFI CCNGG 4 cut(s) 117, 264, 1288, 1355
SduI GDGCHC 2 cut(s) 35, 1003
SexAI ACCWGGT 1 cut(s) 262
SfaNI GCATC 3 cut(s) 482, 601, 1180
SfuI TTCGAA 2 cut(s) 928, 1229
SinI GGWCC 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 8 cut(s) 64, 165, 310, 347, 606, 891, 1157, 1362
SseBI AGGCCT 1 cut(s) 952
SsiI CCGC 1 cut(s) 1034
SspMI CTAG 5 cut(s) 857, 921, 1149, 1305, 1498
SstI GAGCTC 2 cut(s) 35, 1003
StuI AGGCCT 1 cut(s) 952
StyD4I CCNGG 4 cut(s) 115, 262, 1286, 1353
StyI CCWWGG 2 cut(s) 330, 1343
TaaI ACNGT 4 cut(s) 149, 901, 1241, 1432
TaiI ACGT 3 cut(s) 162, 576, 1403
TaqI TCGA 3 cut(s) 681, 928, 1229
TasI AATT 8 cut(s) 64, 165, 310, 347, 606, 891, 1157, 1362
TatI WGTACW 1 cut(s) 1163
TauI GCSGC 1 cut(s) 1036
TfiI GAWTC 8 cut(s) 112, 120, 209, 401, 881, 1144, 1219, 1476
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 1072
TseFI GTSAC 3 cut(s) 160, 443, 664
TseI GCWGC 4 cut(s) 95, 320, 1036, 1103
Tsp45I GTSAC 3 cut(s) 160, 443, 664
TspDTI ATGAA 6 cut(s) 532, 647, 1058, 1149, 1347, 1401
TspRI CASTG 1 cut(s) 1072
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 2 cut(s) 64, 891
XbaI TCTAGA 1 cut(s) 856
XmiI GTMKAC 2 cut(s) 570, 1373
XspI CTAG 5 cut(s) 857, 921, 1149, 1305, 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.