Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002596

Physical Location & Seq

Genomic Coordinates Reverse (-)

32116813 .. 32121108

Gene Structure 4296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1245700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGGCCAAATTCTCAGTACTCTCACTCTGCTGCATGCTTTTTGTGAGTTTGGTCGTGAACTTAATTGAGGCTGGCCCTCGAAGCTCTCTTGTCACTCATCTTCCTGGTTTTAATGCTACCTTTCCATCCAAGCACTACTCTGGATATATAACCACAGATGGAAAGAATCTTTTCTACTACTTCGTAGTTTCAGAAAGGAGCCCAGAAAGTGATCCCGTTGTTCTGTGGCTCAATGGTGGACCTGGCTGCTCTAGCTTCGACGGATTTGTCTATGAACATGGTCATCCGTCATGGGATTGCTCTCGCGCGAACTCACTTAACTTCGGAGTTCCAGAGCTCTCAAAAGGCCTCGTGCTAGGTAGAGATGAGAATATACATATAAGGCTTAGAGGATCCACTCCCCTGTGCAATGTTTCCAACATAATTTATTTGGATTCTCCTGCTGGTGTTGGGTTCTCTTACTCTCAAAACCAAACCAAATATATAACTGGGGACATCCAAACTGCTCATGATACCCATGCCTTTCTCCTCAAGTGGTTTCAGCAATTCCCAGAGTTTCAACCAAACCCGTTGTACATTTCGGGAGAGTCTTATGCTGGAGTGTATGTGCCAACTCTTGCTTCTCAAATTGCAAAAGGTATTAAAAATGGAACAAAACCAACTTTAAATCTGAAGGGTTACCTGGTGGGGAATGGAGTCGCAGACCGAAAATTTGATGCCAATGCTTTAGTCCCATTTGCTCATGGGATGGGTATCATTTCAGAACAAATCTTTCAGGAAATTATAGCTACTTGTGGTACTGACCTCCTTAATATTCCTCCTTCCAATCGTAGTCTATGCAACGAAAAGTTTCAACCATTTAACAAGGCTCTTGACGGACTAAACATCTACGACACCCTTGAGCCTTGTTACCATGGCCCTGGAACAGATAATAGCAAGAATTCCAAAGGAAATAATACAAGCAGCTTACCGTTAAGCTTTCAACAGTTGGGGACGACGACAGAGAAGCCTCTGGGAGTGAGGAAAAGGATGTTTGGCCGAGCCTGGCCCTTTCTAGCAAACGTAGAAGACGGCATTGTCCCATCGTGGCCTCAGCTCGCAAAGACGCTGAATTCCCGAGTTCCTTGTGTTAATGATGAAGTCGCAACGCTATGGCTGAACACTCCAGAAGTTAGGGAAGCAATACATGCAAAACCAGAAAGTGTGGCTGGTCCATGGGAGTTATGCACAGATAGAATAACATATTCACGCGATGCTGGAAGTATGATCCCTTACCACCAAAACCTTACAACCCAAGGTTATAGAGCACTTATTTTCAGTGGAGACCATGATATGTGTGTTCCGTACACTGGGAGTGAGGCATGGACTGCATCACTTGGATATAAGATTGTAGATGAATGGAGACCTTGGCTCTCCTCTGACGACCAAGTTGCTGGCTACTTACAAGGATATGCAAACGACCTCACCTTTCTCACCGTCAAGGGAGCTGGACACACTGTCCCGGAATACAAGCCTCGGGAGGCACTTGACTTCTTCCAACGCTTTTTGGACGAAAAACGCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005777 GO:0008150 GO:0008152 GO:0016740 GO:0016746 GO:0016747 GO:0019748 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

522

Amino Acids

57.7

Weight (kDa)

6.02

Isoelectric Point (pI)

36.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	35 - 99	1.3e-22	Serine carboxypeptidase
Peptidase_S10	PF00450	137 - 517	1.6e-99	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002596)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25420 AT4G12910 AT4G12910
fragaria_vesca	FvH4_2g17690 FvH4_6g00420 FvH4_6g00420 FvH4_6g00420
malus_domestica	MD00G1170000.v1.1 MD04G1245700.v1.1 MD05G1158200.v1.1 MD10G1151200.v1.1 MD12G1264200.v1.1 MD13G1061200.v1.1
prunus_persica	Prupe.6G362200_v2.0.a1 Prupe.8G189600_v2.0.a1
pyrus_communis	pycom04g21660 pycom05g15020 pycom10g13030 pycom12g23930
rosa_chinensis	RchiOBHm_Chr3g0447751 RchiOBHm_Chr6g0282671
rosa_laevigata	RLG00000002738
rosa_multiflora	Rmu_co8425633.1_g000001 Rmu_ssc0000391.1_g000016
rosa_roxburghii	Rroxscaffold_3G00245270 Rroxscaffold_6G00425950
rosa_rugosa	Rorug02G0605300 Rorug06G0148500 Rorug07G0145800
rosa_samantha	Rh3AG005000 Rh3BG004600 Rh3CG005000 Rh3DG004900 Rh6AG261900 Rh6BG265000 Rh6CG264100 Rh6DG256300 Rh7BG273200
rosa_wichuraiana	Rw3G000370 Rw6G022640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	266, 1076
AccII	CGCG	3 cut(s)	306, 308, 1251
AclWI	GGATC	4 cut(s)	206, 387, 400, 1261
AcoI	YGGCCR	2 cut(s)	3, 1036
AcsI	RAATTY	4 cut(s)	8, 710, 940, 1111
AcuI	CTGAAG	1 cut(s)	692
AfaI	GTAC	4 cut(s)	18, 575, 799, 1346
AfiI	CCNNNNNNNGG	1 cut(s)	1349
AgsI	TTSAA	3 cut(s)	560, 854, 983
AhdI	GACNNNNNGTC	1 cut(s)	1496
AjnI	CCWGG	5 cut(s)	103, 241, 681, 919, 1043
AjuI	GAANNNNNNNTTGG	2 cut(s)	604, 636
AluBI	AGCT	8 cut(s)	84, 255, 337, 788, 966, 978, 1096, 1487
AluI	AGCT	8 cut(s)	84, 255, 337, 788, 966, 978, 1096, 1487
Alw21I	GWGCWC	2 cut(s)	339, 1309
Alw26I	GTCTC	2 cut(s)	1317, 1396
AlwI	GGATC	4 cut(s)	206, 387, 400, 1261
Ama87I	CYCGRG	2 cut(s)	1116, 1515
AoxI	GGCC	7 cut(s)	3, 73, 346, 916, 1036, 1046, 1088
ApeKI	GCWGC	3 cut(s)	30, 246, 963
ApoI	RAATTY	4 cut(s)	8, 710, 940, 1111
ArsI	GACNNNNNNTTYG	2 cut(s)	695, 727
Asp700I	GAANNNNTTC	2 cut(s)	170, 849
AspLEI	GCGC	1 cut(s)	308
AspS9I	GGNCC	5 cut(s)	74, 239, 917, 1047, 1211
AsuC2I	CCSGG	1 cut(s)	1502
AsuHPI	GGTGA	2 cut(s)	1456, 1465
AvaI	CYCGRG	2 cut(s)	1116, 1515
AvaII	GGWCC	2 cut(s)	239, 1211
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	392
BanII	GRGCYC	2 cut(s)	203, 339
BauI	CACGAG	1 cut(s)	350
BbsI	GAAGAC	1 cut(s)	1074
Bbv12I	GWGCWC	2 cut(s)	339, 1309
BbvCI	CCTCAGC	1 cut(s)	1092
BbvI	GCAGC	3 cut(s)	17, 233, 975
BccI	CCATC	4 cut(s)	133, 152, 742, 1090
BceAI	ACGGC	1 cut(s)	1087
BcgI	CGANNNNNNTGC	2 cut(s)	1412, 1446
BciT130I	CCWGG	5 cut(s)	105, 243, 683, 921, 1045
BcnI	CCSGG	1 cut(s)	1502
BcoDI	GTCTC	2 cut(s)	1317, 1396
BfaI	CTAG	3 cut(s)	252, 356, 1055
BisI	GCNGC	3 cut(s)	31, 247, 964
BlsI	GCNGC	3 cut(s)	32, 248, 965
BmcAI	AGTACT	1 cut(s)	18
Bme1390I	CCNGG	6 cut(s)	105, 243, 683, 921, 1045, 1502
Bme18I	GGWCC	2 cut(s)	239, 1211
BmeRI	GACNNNNNGTC	1 cut(s)	1496
BmeT110I	CYCGRG	2 cut(s)	1116, 1515
BmgT120I	GGNCC	5 cut(s)	74, 239, 917, 1047, 1211
BmiI	GGNNCC	2 cut(s)	200, 394
BmrFI	CCNGG	6 cut(s)	105, 243, 683, 921, 1045, 1502
BmrI	ACTGGG	2 cut(s)	498, 1359
BmsI	GCATC	3 cut(s)	706, 1243, 1379
BmuI	ACTGGG	2 cut(s)	498, 1359
BpiI	GAAGAC	1 cut(s)	1074
BpmI	CTGGAG	2 cut(s)	618, 1149
Bpu10I	CCTNAGC	1 cut(s)	1092
BpuEI	CTTGAG	2 cut(s)	515, 920
BpuMI	CCSGG	1 cut(s)	1502
BsaBI	GATNNNNATC	1 cut(s)	752
BsaI	GGTCTC	2 cut(s)	1317, 1396
BsaJI	CCNNGG	6 cut(s)	913, 919, 1214, 1294, 1406, 1514
BsaXI	ACNNNNNCTCC	2 cut(s)	591, 621
Bsc4I	CCNNNNNNNGG	1 cut(s)	1349
Bse1I	ACTGG	2 cut(s)	493, 1354
Bse3DI	GCAATG	1 cut(s)	415
Bse8I	GATNNNNATC	1 cut(s)	752
BseBI	CCWGG	5 cut(s)	105, 243, 683, 921, 1045
BseDI	CCNNGG	6 cut(s)	913, 919, 1214, 1294, 1406, 1514
BseGI	GGATG	5 cut(s)	125, 283, 495, 753, 1035
BseJI	GATNNNNATC	1 cut(s)	752
BseLI	CCNNNNNNNGG	1 cut(s)	1349
BseMI	GCAATG	1 cut(s)	415
BseMII	CTCAG	2 cut(s)	27, 1106
BseNI	ACTGG	2 cut(s)	493, 1354
BseRI	GAGGAG	2 cut(s)	518, 1405
BseXI	GCAGC	3 cut(s)	17, 233, 975
Bsh1236I	CGCG	3 cut(s)	306, 308, 1251
BshFI	GGCC	7 cut(s)	5, 75, 348, 918, 1038, 1048, 1090
BsiHKAI	GWGCWC	2 cut(s)	339, 1309
BsiHKCI	CYCGRG	2 cut(s)	1116, 1515
BsiSI	CCGG	1 cut(s)	1502
BslFI	GGGAC	5 cut(s)	506, 716, 1006, 1064, 1484
BslI	CCNNNNNNNGG	1 cut(s)	1349
BsmAI	GTCTC	2 cut(s)	1317, 1396
BsmFI	GGGAC	5 cut(s)	506, 716, 1006, 1064, 1484
BsnI	GGCC	7 cut(s)	5, 75, 348, 918, 1038, 1048, 1090
Bso31I	GGTCTC	2 cut(s)	1317, 1396
BsoBI	CYCGRG	2 cut(s)	1116, 1515
Bsp1286I	GDGCHC	3 cut(s)	203, 339, 1309
Bsp1407I	TGTACA	1 cut(s)	573
Bsp143I	GATC	3 cut(s)	211, 392, 1266
Bsp19I	CCATGG	2 cut(s)	913, 1214
BspANI	GGCC	7 cut(s)	5, 75, 348, 918, 1038, 1048, 1090
BspCNI	CTCAG	2 cut(s)	26, 1105
BspFNI	CGCG	3 cut(s)	306, 308, 1251
BspHI	TCATGA	1 cut(s)	508
BspLI	GGNNCC	2 cut(s)	200, 394
BspPI	GGATC	4 cut(s)	206, 387, 400, 1261
BspTNI	GGTCTC	2 cut(s)	1317, 1396
BsrDI	GCAATG	1 cut(s)	415
BsrGI	TGTACA	1 cut(s)	573
BsrI	ACTGG	2 cut(s)	493, 1354
BssECI	CCNNGG	6 cut(s)	913, 919, 1214, 1294, 1406, 1514
BssMI	GATC	3 cut(s)	211, 392, 1266
BssSI	CACGAG	1 cut(s)	350
BssT1I	CCWWGG	4 cut(s)	913, 1214, 1294, 1406
Bst2BI	CACGAG	1 cut(s)	350
Bst2UI	CCWGG	5 cut(s)	105, 243, 683, 921, 1045
Bst4CI	ACNGT	4 cut(s)	972, 987, 1477, 1498
BstAPI	GCANNNNNTGC	2 cut(s)	1187, 1367
BstAUI	TGTACA	1 cut(s)	573
BstC8I	GCNNGC	4 cut(s)	35, 73, 1098, 1435
BstDEI	CTNAG	3 cut(s)	13, 386, 1092
BstDSI	CCRYGG	2 cut(s)	913, 1214
BstEII	GGTNACC	1 cut(s)	677
BstF5I	GGATG	5 cut(s)	125, 283, 495, 753, 1035
BstFNI	CGCG	3 cut(s)	306, 308, 1251
BstHHI	GCGC	1 cut(s)	308
BstKTI	GATC	3 cut(s)	214, 395, 1269
BstMAI	GTCTC	2 cut(s)	1317, 1396
BstMBI	GATC	3 cut(s)	211, 392, 1266
BstMWI	GCNNNNNNNGC	4 cut(s)	81, 252, 1187, 1367
BstNI	CCWGG	5 cut(s)	105, 243, 683, 921, 1045
BstNSI	RCATGY	2 cut(s)	37, 1190
BstPI	GGTNACC	1 cut(s)	677
BstSCI	CCNGG	6 cut(s)	103, 241, 681, 919, 1043, 1500
BstUI	CGCG	3 cut(s)	306, 308, 1251
BstV1I	GCAGC	3 cut(s)	17, 233, 975
BstV2I	GAAGAC	1 cut(s)	1074
BstX2I	RGATCY	1 cut(s)	392
BstXI	CCANNNNNNTGG	2 cut(s)	920, 1433
BstYI	RGATCY	1 cut(s)	392
BsuRI	GGCC	7 cut(s)	5, 75, 348, 918, 1038, 1048, 1090
BtgI	CCRYGG	2 cut(s)	913, 1214
BtgZI	GCGATG	1 cut(s)	1266
BtsCI	GGATG	5 cut(s)	125, 283, 495, 753, 1035
BtsIMutI	CAGTG	3 cut(s)	1324, 1347, 1494
Cac8I	GCNNGC	4 cut(s)	35, 73, 1098, 1435
CciI	TCATGA	1 cut(s)	508
CfoI	GCGC	1 cut(s)	308
Cfr13I	GGNCC	5 cut(s)	74, 239, 917, 1047, 1211
CseI	GACGC	1 cut(s)	1114
CsiI	ACCWGGT	1 cut(s)	681
Csp6I	GTAC	4 cut(s)	17, 574, 798, 1345
CviQI	GTAC	4 cut(s)	17, 574, 798, 1345
DdeI	CTNAG	3 cut(s)	13, 386, 1092
DpnI	GATC	3 cut(s)	213, 394, 1268
DpnII	GATC	3 cut(s)	211, 392, 1266
DraI	TTTAAA	1 cut(s)	666
DrdI	GACNNNNNNGTC	2 cut(s)	266, 1076
DriI	GACNNNNNGTC	1 cut(s)	1496
DseDI	GACNNNNNNGTC	2 cut(s)	266, 1076
EaeI	YGGCCR	2 cut(s)	3, 1036
Eam1105I	GACNNNNNGTC	1 cut(s)	1496
Ecl136II	GAGCTC	1 cut(s)	337
Eco130I	CCWWGG	4 cut(s)	913, 1214, 1294, 1406
Eco147I	AGGCCT	1 cut(s)	348
Eco24I	GRGCYC	2 cut(s)	203, 339
Eco31I	GGTCTC	2 cut(s)	1317, 1396
Eco47I	GGWCC	2 cut(s)	239, 1211
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	1 cut(s)	692
Eco88I	CYCGRG	2 cut(s)	1116, 1515
Eco91I	GGTNACC	1 cut(s)	677
EcoICRI	GAGCTC	1 cut(s)	337
EcoO65I	GGTNACC	1 cut(s)	677
EcoRI	GAATTC	2 cut(s)	940, 1111
EcoRII	CCWGG	5 cut(s)	103, 241, 681, 919, 1043
EcoT14I	CCWWGG	4 cut(s)	913, 1214, 1294, 1406
EcoT38I	GRGCYC	2 cut(s)	203, 339
ErhI	CCWWGG	4 cut(s)	913, 1214, 1294, 1406
FaqI	GGGAC	5 cut(s)	506, 716, 1006, 1064, 1484
Fnu4HI	GCNGC	3 cut(s)	31, 247, 964
FokI	GGATG	5 cut(s)	112, 270, 482, 760, 1042
FriOI	GRGCYC	2 cut(s)	203, 339
Fsp4HI	GCNGC	3 cut(s)	31, 247, 964
FspBI	CTAG	3 cut(s)	252, 356, 1055
GlaI	GCGC	1 cut(s)	307
GluI	GCNGC	3 cut(s)	31, 247, 964
GsuI	CTGGAG	2 cut(s)	618, 1149
HaeIII	GGCC	7 cut(s)	5, 75, 348, 918, 1038, 1048, 1090
HapII	CCGG	1 cut(s)	1502
HgaI	GACGC	1 cut(s)	1114
HhaI	GCGC	1 cut(s)	308
Hin6I	GCGC	1 cut(s)	306
HinP1I	GCGC	1 cut(s)	306
HindIII	AAGCTT	1 cut(s)	976
HinfI	GANTC	4 cut(s)	166, 434, 587, 696
HpaII	CCGG	1 cut(s)	1502
HphI	GGTGA	2 cut(s)	1456, 1465
Hpy166II	GTNNAC	3 cut(s)	58, 239, 1347
Hpy188I	TCNGA	5 cut(s)	193, 326, 672, 763, 1420
Hpy8I	GTNNAC	3 cut(s)	58, 239, 1347
Hpy99I	CGWCG	2 cut(s)	263, 1000
HpyAV	CCTTC	2 cut(s)	667, 831
HpyCH4III	ACNGT	4 cut(s)	972, 987, 1477, 1498
HpyCH4IV	ACGT	1 cut(s)	1062
HpyCH4V	TGCA	8 cut(s)	33, 408, 632, 840, 1190, 1227, 1370, 1454
HpyF10VI	GCNNNNNNNGC	4 cut(s)	81, 252, 1187, 1367
HpyF3I	CTNAG	3 cut(s)	13, 386, 1092
HpySE526I	ACGT	1 cut(s)	1062
HspAI	GCGC	1 cut(s)	306
Kzo9I	GATC	3 cut(s)	211, 392, 1266
LmnI	GCTCC	2 cut(s)	198, 1484
Lsp1109I	GCAGC	3 cut(s)	17, 233, 975
LweI	GCATC	3 cut(s)	706, 1243, 1379
MabI	ACCWGGT	1 cut(s)	681
MaeI	CTAG	3 cut(s)	252, 356, 1055
MaeII	ACGT	1 cut(s)	1062
MaeIII	GTNAC	3 cut(s)	91, 677, 908
MalI	GATC	3 cut(s)	213, 394, 1268
MboI	GATC	3 cut(s)	211, 392, 1266
MboII	GAAGA	3 cut(s)	92, 1079, 1525
MflI	RGATCY	1 cut(s)	392
MhlI	GDGCHC	3 cut(s)	203, 339, 1309
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	9 cut(s)	8, 63, 423, 545, 627, 710, 780, 940, 1111
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	596, 705
MmeI	TCCRAC	2 cut(s)	441, 1561
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	2 cut(s)	170, 849
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	9 cut(s)	62, 111, 318, 642, 665, 810, 861, 974, 1131
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	1 cut(s)	1502
MspR9I	CCNGG	6 cut(s)	105, 243, 683, 921, 1045, 1502
MvaI	CCWGG	5 cut(s)	105, 243, 683, 921, 1045
MvnI	CGCG	3 cut(s)	306, 308, 1251
MwoI	GCNNNNNNNGC	4 cut(s)	81, 252, 1187, 1367
NciI	CCSGG	1 cut(s)	1502
NcoI	CCATGG	2 cut(s)	913, 1214
NdeII	GATC	3 cut(s)	211, 392, 1266
NlaIV	GGNNCC	2 cut(s)	200, 394
NmeAIII	GCCGAG	1 cut(s)	1064
NmuCI	GTSAC	1 cut(s)	91
NspI	RCATGY	2 cut(s)	37, 1190
PaeI	GCATGC	1 cut(s)	37
PagI	TCATGA	1 cut(s)	508
PceI	AGGCCT	1 cut(s)	348
PdmI	GAANNNNTTC	2 cut(s)	170, 849
PfeI	GAWTC	2 cut(s)	166, 434
PfoI	TCCNGGA	1 cut(s)	1500
PkrI	GCNGC	3 cut(s)	32, 248, 965
PleI	GAGTC	2 cut(s)	595, 704
PpsI	GAGTC	2 cut(s)	595, 704
Psp124BI	GAGCTC	1 cut(s)	339
Psp6I	CCWGG	5 cut(s)	103, 241, 681, 919, 1043
PspEI	GGTNACC	1 cut(s)	677
PspGI	CCWGG	5 cut(s)	103, 241, 681, 919, 1043
PspN4I	GGNNCC	2 cut(s)	200, 394
PspPI	GGNCC	5 cut(s)	74, 239, 917, 1047, 1211
PsuI	RGATCY	1 cut(s)	392
RsaI	GTAC	4 cut(s)	18, 575, 799, 1346
RsaNI	GTAC	4 cut(s)	17, 574, 798, 1345
SacI	GAGCTC	1 cut(s)	339
SaqAI	TTAA	9 cut(s)	62, 111, 318, 642, 665, 810, 861, 974, 1131
SatI	GCNGC	3 cut(s)	31, 247, 964
Sau3AI	GATC	3 cut(s)	211, 392, 1266
Sau96I	GGNCC	5 cut(s)	74, 239, 917, 1047, 1211
ScaI	AGTACT	1 cut(s)	18
SchI	GAGTC	2 cut(s)	596, 705
ScrFI	CCNGG	6 cut(s)	105, 243, 683, 921, 1045, 1502
SduI	GDGCHC	3 cut(s)	203, 339, 1309
SexAI	ACCWGGT	1 cut(s)	681
SfaNI	GCATC	3 cut(s)	706, 1243, 1379
SinI	GGWCC	2 cut(s)	239, 1211
SmlI	CTYRAG	2 cut(s)	530, 899
SmoI	CTYRAG	2 cut(s)	530, 899
SphI	GCATGC	1 cut(s)	37
Sse9I	AATT	9 cut(s)	8, 63, 423, 545, 627, 710, 780, 940, 1111
SseBI	AGGCCT	1 cut(s)	348
SspI	AATATT	1 cut(s)	814
SspMI	CTAG	3 cut(s)	252, 356, 1055
SstI	GAGCTC	1 cut(s)	339
StuI	AGGCCT	1 cut(s)	348
StyD4I	CCNGG	6 cut(s)	103, 241, 681, 919, 1043, 1500
StyI	CCWWGG	4 cut(s)	913, 1214, 1294, 1406
TaaI	ACNGT	4 cut(s)	972, 987, 1477, 1498
TaiI	ACGT	1 cut(s)	1065
TaqI	TCGA	2 cut(s)	79, 258
TaqII	GACCGA	1 cut(s)	720
TasI	AATT	9 cut(s)	8, 63, 423, 545, 627, 710, 780, 940, 1111
TatI	WGTACW	2 cut(s)	16, 573
TfiI	GAWTC	2 cut(s)	166, 434
Tru1I	TTAA	9 cut(s)	62, 111, 318, 642, 665, 810, 861, 974, 1131
Tru9I	TTAA	9 cut(s)	62, 111, 318, 642, 665, 810, 861, 974, 1131
TscAI	CASTG	3 cut(s)	1324, 1354, 1501
TseFI	GTSAC	1 cut(s)	91
TseI	GCWGC	3 cut(s)	30, 246, 963
Tsp45I	GTSAC	1 cut(s)	91
TspDTI	ATGAA	3 cut(s)	288, 1152, 1410
TspGWI	ACGGA	4 cut(s)	276, 276, 891, 1332
TspRI	CASTG	3 cut(s)	1324, 1354, 1501
VpaK11BI	GGWCC	2 cut(s)	239, 1211
XapI	RAATTY	4 cut(s)	8, 710, 940, 1111
XceI	RCATGY	2 cut(s)	37, 1190
XmnI	GAANNNNTTC	2 cut(s)	170, 849
XspI	CTAG	3 cut(s)	252, 356, 1055
ZrmI	AGTACT	1 cut(s)	18

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.