Rw3G000370

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002596
Physical Location & Seq
Forward (+)
361354 .. 365759
4406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G000370.1

Sequence Viewer

Length: 1113 bp
ATGGCGGGCATGGCCAGACCGTTGGCTACAATTCCCACCTGCTTCTCAATCATAATCATATGCTTAAGCTTGATAACAATGAGAATTGAGGCAGCCCCTCAAGGCTCTCTTGTCACCCAGCTTCCCGGCTTCAATGGCACTTTTCCGTCCAGCCACTATTCCGGATATGTAAGCGTAGGCGCAAAGAAACTTTTCTACTACTTCGTAGTGTCAGAAGGGAGCCCCGCAGACGATCCTGTTGTCTTGTGGCTCAATGGCGGACCCGGCTGCTCTAGCTTTGACGGATTCGTCTACGAACACGGGCCGTTCAATTTTGAACAAAAATCGAAAGGAGCATTGCCCACTTTACAACTTAATCCTTACAGCTGGTCCAAGGTTTCCAACATGCTGTATTTGGATTCTCCTGCTGGTGTTGGGTTTTCCTACTCAGAAGACCCAAGCCAATACGTAACTGGGGATCTTCAAACTGCTGCCGACACCCACGCCTTCCTCCTCAGCTGGTTTAAGCAGTACCCGGAGTTTCTTTCCAATCCCTTTTACATTTCCGGGGAGTCTTATGCTGGAGTCTACGTCCCTACTCTTGCCTTTCAAGTTGCCACAGGTATTCAAAATGGAACAAAACCAGTTGTAAATCTCAAGGGTTACATGGTGGGGAATGGAGTCACAGACAGAAACTTTGATGACAATGCTCTTGTCCCTTTTGCTCATGGGATGGCCCTCATTTCAGATCAAATTTATGAGGGAGTTATAGAAGAATGTGGGATCAACTACTCCAACTCATCTAGTCCCAAGTGCAATGAGAAACTTAATCTGGCTCGCGAGGCTCTTGGTGGACTAAATATCTACAACATCCTTGAGCCTTGTTACCACGGAACTGAATCAGGAAATGATACTTCTACAACAACCAAAAGCAGCAACTTACCCCTTAGCTTTCAGCAACTAGGCAAGTCGCCTGAGAAGCCTCTCGGAGTGAGAAAGAGGATGTTTGGCCGCGCTTGGCCTTATCAGATGTCAGTAGAAGATGGCTTAGTCCCTTTGTGGCCACAGCTAGCTGCTAAATCTAACTCACGCACTGTTCCATGCTTTGTAAGTAATTCACACTTCTATGGTTGA

Protein Analysis

370

Amino Acids

40.3

Weight (kDa)

5.54

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 42 - 303 4.5e-91 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
AasI GACNNNNNNGTC 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 47
AccI GTMKAC 2 cut(s) 291, 567
AccII CGCG 2 cut(s) 819, 993
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 4 cut(s) 5, 225, 258, 991
AclWI GGATC 3 cut(s) 227, 465, 770
AcoI YGGCCR 3 cut(s) 12, 988, 1040
AcsI RAATTY 1 cut(s) 732
AfaI GTAC 1 cut(s) 512
AflII CTTAAG 1 cut(s) 64
AgsI TTSAA 6 cut(s) 133, 310, 317, 464, 590, 608
AluBI AGCT 8 cut(s) 69, 121, 276, 366, 498, 930, 1048, 1052
AluI AGCT 8 cut(s) 69, 121, 276, 366, 498, 930, 1048, 1052
AlwI GGATC 3 cut(s) 227, 465, 770
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 6 cut(s) 12, 302, 714, 988, 998, 1040
ApeKI GCWGC 5 cut(s) 92, 267, 470, 912, 1052
ApoI RAATTY 1 cut(s) 732
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
Asp700I GAANNNNTTC 1 cut(s) 191
AspLEI GCGC 2 cut(s) 182, 995
AspS9I GGNCC 4 cut(s) 260, 302, 369, 715
AsuC2I CCSGG 4 cut(s) 126, 264, 515, 547
AsuHPI GGTGA 1 cut(s) 106
AsuNHI GCTAGC 1 cut(s) 1048
AvaII GGWCC 2 cut(s) 260, 369
BalI TGGCCA 2 cut(s) 14, 1042
BanII GRGCYC 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 5 cut(s) 104, 254, 457, 924, 1039
BccI CCATC 2 cut(s) 706, 1016
BceAI ACGGC 1 cut(s) 289
BcnI CCSGG 4 cut(s) 126, 264, 515, 547
BfaI CTAG 4 cut(s) 273, 783, 941, 1049
BfrI CTTAAG 1 cut(s) 64
BfuAI ACCTGC 1 cut(s) 47
BisI GCNGC 6 cut(s) 93, 268, 471, 913, 991, 1053
BlsI GCNGC 6 cut(s) 94, 269, 472, 914, 992, 1054
Bme1390I CCNGG 4 cut(s) 126, 264, 515, 547
Bme18I GGWCC 2 cut(s) 260, 369
BmgT120I GGNCC 4 cut(s) 260, 302, 369, 715
BmiI GGNNCC 2 cut(s) 221, 262
BmrFI CCNGG 4 cut(s) 126, 264, 515, 547
BmrI ACTGGG 1 cut(s) 462
BmtI GCTAGC 1 cut(s) 1052
BmuI ACTGGG 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 2 cut(s) 494, 926
BpuEI CTTGAG 3 cut(s) 84, 620, 875
BpuMI CCSGG 4 cut(s) 126, 264, 515, 547
BsaAI YACGTR 1 cut(s) 448
BsaJI CCNNGG 3 cut(s) 372, 546, 868
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bse1I ACTGG 2 cut(s) 457, 623
Bse3DI GCAATG 2 cut(s) 335, 802
BseAI TCCGGA 1 cut(s) 161
BseDI CCNNGG 3 cut(s) 372, 546, 868
BseGI GGATG 3 cut(s) 717, 849, 987
BseMI GCAATG 2 cut(s) 335, 802
BseMII CTCAG 3 cut(s) 441, 508, 945
BseNI ACTGG 2 cut(s) 457, 623
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 5 cut(s) 104, 254, 457, 924, 1039
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 819, 993
BshFI GGCC 6 cut(s) 14, 304, 716, 990, 1000, 1042
BsiSI CCGG 5 cut(s) 126, 162, 264, 515, 546
BslFI GGGAC 4 cut(s) 557, 680, 771, 1016
BsmFI GGGAC 4 cut(s) 557, 680, 771, 1016
BsnI GGCC 6 cut(s) 14, 304, 716, 990, 1000, 1042
Bsp1286I GDGCHC 1 cut(s) 224
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 4 cut(s) 232, 457, 727, 762
Bsp68I TCGCGA 1 cut(s) 819
BspACI CCGC 4 cut(s) 5, 225, 258, 991
BspANI GGCC 6 cut(s) 14, 304, 716, 990, 1000, 1042
BspCNI CTCAG 3 cut(s) 440, 507, 946
BspEI TCCGGA 1 cut(s) 161
BspFNI CGCG 2 cut(s) 819, 993
BspLI GGNNCC 2 cut(s) 221, 262
BspMI ACCTGC 1 cut(s) 47
BspOI GCTAGC 1 cut(s) 1052
BspPI GGATC 3 cut(s) 227, 465, 770
BspTI CTTAAG 1 cut(s) 64
BsrDI GCAATG 2 cut(s) 335, 802
BsrI ACTGG 2 cut(s) 457, 623
BssECI CCNNGG 3 cut(s) 372, 546, 868
BssMI GATC 4 cut(s) 232, 457, 727, 762
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 21, 1075
BstAFI CTTAAG 1 cut(s) 64
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 3 cut(s) 7, 817, 1050
BstDEI CTNAG 5 cut(s) 427, 494, 926, 954, 1027
BstDSI CCRYGG 1 cut(s) 868
BstF5I GGATG 3 cut(s) 717, 849, 987
BstFNI CGCG 2 cut(s) 819, 993
BstHHI GCGC 2 cut(s) 182, 995
BstKTI GATC 4 cut(s) 235, 460, 730, 765
BstMBI GATC 4 cut(s) 232, 457, 727, 762
BstMWI GCNNNNNNNGC 6 cut(s) 11, 135, 264, 273, 821, 958
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 4 cut(s) 124, 262, 513, 545
BstSNI TACGTA 1 cut(s) 448
BstUI CGCG 2 cut(s) 819, 993
BstV1I GCAGC 5 cut(s) 104, 254, 457, 924, 1039
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 457
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 6 cut(s) 14, 304, 716, 990, 1000, 1042
BtgI CCRYGG 1 cut(s) 868
BtsCI GGATG 3 cut(s) 717, 849, 987
BtsIMutI CAGTG 1 cut(s) 1071
BtuMI TCGCGA 1 cut(s) 819
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 3 cut(s) 7, 817, 1050
CfoI GCGC 2 cut(s) 182, 995
Cfr13I GGNCC 4 cut(s) 260, 302, 369, 715
Csp6I GTAC 1 cut(s) 511
CviAII CATG 5 cut(s) 10, 385, 646, 707, 1080
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 5 cut(s) 427, 494, 926, 954, 1027
DpnI GATC 4 cut(s) 234, 459, 729, 764
DpnII GATC 4 cut(s) 232, 457, 727, 762
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 3 cut(s) 12, 988, 1040
EciI GGCGGA 1 cut(s) 273
Eco105I TACGTA 1 cut(s) 448
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 260, 369
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 5 cut(s) 13, 388, 649, 710, 1083
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 4 cut(s) 557, 680, 771, 1016
FatI CATG 5 cut(s) 9, 384, 645, 706, 1079
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 59
FblI GTMKAC 2 cut(s) 291, 567
Fnu4HI GCNGC 6 cut(s) 93, 268, 471, 913, 991, 1053
FokI GGATG 3 cut(s) 724, 836, 994
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 6 cut(s) 93, 268, 471, 913, 991, 1053
FspBI CTAG 4 cut(s) 273, 783, 941, 1049
GlaI GCGC 2 cut(s) 181, 994
GluI GCNGC 6 cut(s) 93, 268, 471, 913, 991, 1053
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 6 cut(s) 14, 304, 716, 990, 1000, 1042
HapII CCGG 5 cut(s) 126, 162, 264, 515, 546
HhaI GCGC 2 cut(s) 182, 995
Hin1II CATG 5 cut(s) 13, 388, 649, 710, 1083
Hin6I GCGC 2 cut(s) 180, 993
HinP1I GCGC 2 cut(s) 180, 993
HindIII AAGCTT 1 cut(s) 67
HinfI GANTC 6 cut(s) 285, 398, 551, 564, 660, 878
HpaII CCGG 5 cut(s) 126, 162, 264, 515, 546
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 292, 568, 833
Hpy188I TCNGA 5 cut(s) 214, 430, 727, 968, 1008
Hpy188III TCNNGA 3 cut(s) 162, 818, 882
Hpy8I GTNNAC 3 cut(s) 292, 568, 833
HpyAV CCTTC 2 cut(s) 209, 496
HpyCH4III ACNGT 2 cut(s) 21, 1075
HpyCH4IV ACGT 2 cut(s) 447, 570
HpyCH4V TGCA 1 cut(s) 795
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 135, 264, 273, 821, 958
HpyF3I CTNAG 5 cut(s) 427, 494, 926, 954, 1027
HpySE526I ACGT 2 cut(s) 447, 570
Hsp92II CATG 5 cut(s) 13, 388, 649, 710, 1083
HspAI GCGC 2 cut(s) 180, 993
Kpn2I TCCGGA 1 cut(s) 161
Kzo9I GATC 4 cut(s) 232, 457, 727, 762
LmnI GCTCC 2 cut(s) 219, 332
Lsp1109I GCAGC 5 cut(s) 104, 254, 457, 924, 1039
MaeI CTAG 4 cut(s) 273, 783, 941, 1049
MaeII ACGT 2 cut(s) 447, 570
MaeIII GTNAC 5 cut(s) 112, 448, 641, 661, 863
MalI GATC 4 cut(s) 234, 459, 729, 764
MboI GATC 4 cut(s) 232, 457, 727, 762
MboII GAAGA 4 cut(s) 443, 452, 764, 1031
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 2 cut(s) 14, 1042
MluCI AATT 5 cut(s) 30, 84, 310, 732, 1093
MluNI TGGCCA 2 cut(s) 14, 1042
MlyI GAGTC 3 cut(s) 560, 573, 669
MmeI TCCRAC 2 cut(s) 405, 798
MnlI CCTC 9 cut(s) 82, 108, 500, 503, 728, 733, 814, 972, 972
Mox20I TGGCCA 2 cut(s) 14, 1042
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 191
MscI TGGCCA 2 cut(s) 14, 1042
MseI TTAA 4 cut(s) 65, 354, 504, 807
MslI CAYNNNNRTG 1 cut(s) 1104
Msp20I TGGCCA 2 cut(s) 14, 1042
MspA1I CMGCKG 2 cut(s) 366, 498
MspCI CTTAAG 1 cut(s) 64
MspI CCGG 5 cut(s) 126, 162, 264, 515, 546
MspR9I CCNGG 4 cut(s) 126, 264, 515, 547
MvnI CGCG 2 cut(s) 819, 993
MwoI GCNNNNNNNGC 6 cut(s) 11, 135, 264, 273, 821, 958
NciI CCSGG 4 cut(s) 126, 264, 515, 547
NdeI CATATG 1 cut(s) 59
NdeII GATC 4 cut(s) 232, 457, 727, 762
NheI GCTAGC 1 cut(s) 1048
NlaIII CATG 5 cut(s) 13, 388, 649, 710, 1083
NlaIV GGNNCC 2 cut(s) 221, 262
NmuCI GTSAC 2 cut(s) 112, 661
NruI TCGCGA 1 cut(s) 819
NspI RCATGY 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 3 cut(s) 285, 398, 878
PkrI GCNGC 6 cut(s) 94, 269, 472, 914, 992, 1054
PleI GAGTC 3 cut(s) 559, 572, 668
PpsI GAGTC 3 cut(s) 559, 572, 668
Ppu21I YACGTR 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 221, 262
PspPI GGNCC 4 cut(s) 260, 302, 369, 715
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 2 cut(s) 366, 498
RruI TCGCGA 1 cut(s) 819
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 1 cut(s) 1104
SaqAI TTAA 4 cut(s) 65, 354, 504, 807
SatI GCNGC 6 cut(s) 93, 268, 471, 913, 991, 1053
Sau3AI GATC 4 cut(s) 232, 457, 727, 762
Sau96I GGNCC 4 cut(s) 260, 302, 369, 715
SchI GAGTC 3 cut(s) 560, 573, 669
ScrFI CCNGG 4 cut(s) 126, 264, 515, 547
SduI GDGCHC 1 cut(s) 224
SinI GGWCC 2 cut(s) 260, 369
SmiMI CAYNNNNRTG 1 cut(s) 1104
SmlI CTYRAG 4 cut(s) 64, 99, 635, 854
SmoI CTYRAG 4 cut(s) 64, 99, 635, 854
SnaBI TACGTA 1 cut(s) 448
Sse9I AATT 5 cut(s) 30, 84, 310, 732, 1093
SsiI CCGC 4 cut(s) 5, 225, 258, 991
SspMI CTAG 4 cut(s) 273, 783, 941, 1049
StyD4I CCNGG 4 cut(s) 124, 262, 513, 545
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 21, 1075
TaiI ACGT 2 cut(s) 450, 573
TaqI TCGA 1 cut(s) 326
TasI AATT 5 cut(s) 30, 84, 310, 732, 1093
TauI GCSGC 1 cut(s) 993
TfiI GAWTC 3 cut(s) 285, 398, 878
Tru1I TTAA 4 cut(s) 65, 354, 504, 807
Tru9I TTAA 4 cut(s) 65, 354, 504, 807
TscAI CASTG 1 cut(s) 1078
TseFI GTSAC 2 cut(s) 112, 661
TseI GCWGC 5 cut(s) 92, 267, 470, 912, 1052
Tsp45I GTSAC 2 cut(s) 112, 661
TspGWI ACGGA 3 cut(s) 135, 297, 885
TspRI CASTG 1 cut(s) 1078
Vha464I CTTAAG 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 260, 369
XapI RAATTY 1 cut(s) 732
XceI RCATGY 1 cut(s) 388
XcmI CCANNNNNNNNNTGG 1 cut(s) 449
XmiI GTMKAC 2 cut(s) 291, 567
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 4 cut(s) 273, 783, 941, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.