Rh7BG273200

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002596
Physical Location & Seq
Reverse (-)
25270652 .. 25272097
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG273200.1

Sequence Viewer

Length: 1446 bp
ATGGCGGGCATGGCCAGACTGTTGGGTACAATTCCCACCTGCTTCTCAATCATAATAATATGCTTAAGCTTGATAACAATGAGAATTGAGGCAGCCCCTCAGGGCTCTCTTGTCACCCAGCTTCCCGGCTTCAATGGCACTTTTCCGTCCAGCCACTATTCCGGATATGTAAGCATAGGCGCAAAGAATCTTTTCTACTACTTCGTAGTATCAGAAGGGAGCCCCGCAGACGATCCTCTTGTCTTGTGGCTCAATGGTGGACCCGGCTGCTCTAGCTTTGATGGATTCGTCTACGAACATGGGCCTTTCAATTTTGAACAAAAATCAAAAGAAGCATTCCCCACTTTACAGCTTAATCCTTACAGCTGGTCCAAGGTTTCCAACATACTGTATTTGGATTCTCCTGCTGGTGTTGGGTTTTCCTACTCACAAGACCCAAGCCAATATGTAACCGGGGATCTTCAAACTGCTTCCGACACCCACGCCTTCCTCCTCAGCTGGTTTAAGCAGTACCCCGAGTTTCTTTCCAATCCTTTTTACATTTCCGGGGAGTCTTATGCTGGAGTCTACGTCCCTACTCTTGACTTTCAAGTTGCCACAGGTATACAAAATGGAACAAAACCAGTTGTAAATCTCAAGGGTTACATGGTGGGGAATGGAATCACAAACACAAACTTTGATGACAATGCTCTTGTCCCTTTTGCTCATGGGATGGCCCTCATTTCAGATCAAATCTATGAGGGAGTTATAGAAGAATGTGGGATCAACTACTCCAACTCATCTAGTCCCAAGTGCGATGAGAAACTTCAACTGGTTCGCGAGGCTCTTGGTGGACTAAACATGTACGATGTACTTGAGCCTTGTTACCACGACCCAGCTGGAACTTATTCAGGAAATGCTACTTCTACAACAACCAAAAGCAGCAACTTACCCTTAAGCTTCCAGCAACTAGGCAAGTCGTCTGAGAAGCCTCTCGGAGTGAGGAAGAGGATGTCACTCAATGTTCCATGCTTTAATGATGAAGTCGCAACTAAATGGCTGAACGACCCAGCAGTCAGGGAAGCAATTCATGCCAAGCCTGCAAGTGTGTCTGGTCCCTGGGAATTATGCACAGATAGAATAAGTTATAATTTTGGTGCTGGAAGTATGATCCCATACCACCGAAACCTTACAACCAAAGGATATAAAGCAATCATTTTCAGTGGAGACCATGATATGTCTGTGCCGTATACTGGAAGCCAGGCATGGACTAGTTCACTTGGTTATAAGGTTATAGACCAATGGAGGTCTTGGCGATCCAATGACCAAGTTGCAGGTTACTTGCAAGCATATGAAAACGACCTCACCTTTCTCACTGTCAAGGGAGCTGGGCACACCGTTCCTGAATACAAGCCACGAGAGGCACTCGATTTCTACAGCCGATGGTTGGCAGGAAAACCGATATGA

Protein Analysis

481

Amino Acids

52.87

Weight (kDa)

5.41

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 42 - 477 1.2e-134 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1128, 1266
AarI CACCTGC 1 cut(s) 47
AasI GACNNNNNNGTC 1 cut(s) 1052
Acc36I ACCTGC 2 cut(s) 47, 1304
AccI GTMKAC 4 cut(s) 291, 567, 604, 1229
AccII CGCG 1 cut(s) 819
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 2 cut(s) 5, 225
AclWI GGATC 5 cut(s) 227, 465, 770, 1144, 1290
AcoI YGGCCR 1 cut(s) 12
AfaI GTAC 4 cut(s) 28, 512, 845, 852
AfiI CCNNNNNNNGG 2 cut(s) 1232, 1426
AflII CTTAAG 2 cut(s) 64, 934
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 6 cut(s) 133, 310, 317, 464, 590, 809
AhlI ACTAGT 1 cut(s) 1250
AjnI CCWGG 2 cut(s) 1097, 1239
AluBI AGCT 9 cut(s) 69, 121, 276, 352, 366, 498, 878, 939, 1367
AluI AGCT 9 cut(s) 69, 121, 276, 352, 366, 498, 878, 939, 1367
Alw26I GTCTC 1 cut(s) 1200
AlwI GGATC 5 cut(s) 227, 465, 770, 1144, 1290
Ama87I CYCGRG 1 cut(s) 515
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 3 cut(s) 12, 302, 714
ApeKI GCWGC 3 cut(s) 92, 267, 921
Asp700I GAANNNNTTC 3 cut(s) 191, 886, 1065
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 5 cut(s) 260, 302, 369, 715, 1094
AsuC2I CCSGG 4 cut(s) 126, 264, 454, 547
AsuHPI GGTGA 2 cut(s) 106, 1336
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 3 cut(s) 260, 369, 1094
AxyI CCTNAGG 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 1374
BalI TGGCCA 1 cut(s) 14
BanII GRGCYC 2 cut(s) 107, 224
BauI CACGAG 1 cut(s) 1395
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 3 cut(s) 104, 254, 933
BccI CCATC 3 cut(s) 275, 706, 1416
BceAI ACGGC 1 cut(s) 1210
BciT130I CCWGG 2 cut(s) 1099, 1241
BcnI CCSGG 4 cut(s) 126, 264, 454, 547
BcoDI GTCTC 1 cut(s) 1200
BcuI ACTAGT 1 cut(s) 1250
BfaI CTAG 4 cut(s) 273, 783, 950, 1251
BfmI CTRYAG 1 cut(s) 1414
BfrI CTTAAG 2 cut(s) 64, 934
BfuAI ACCTGC 2 cut(s) 47, 1304
BisI GCNGC 3 cut(s) 93, 268, 922
BlsI GCNGC 3 cut(s) 94, 269, 923
Bme1390I CCNGG 6 cut(s) 126, 264, 454, 547, 1099, 1241
Bme18I GGWCC 3 cut(s) 260, 369, 1094
BmeT110I CYCGRG 1 cut(s) 515
BmgT120I GGNCC 5 cut(s) 260, 302, 369, 715, 1094
BmiI GGNNCC 3 cut(s) 221, 262, 1096
BmrFI CCNGG 6 cut(s) 126, 264, 454, 547, 1099, 1241
BplI GAGNNNNNCTC 2 cut(s) 1389, 1421
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 494
BpuEI CTTGAG 2 cut(s) 620, 875
BpuMI CCSGG 4 cut(s) 126, 264, 454, 547
BsaI GGTCTC 1 cut(s) 1200
BsaJI CCNNGG 5 cut(s) 372, 453, 546, 1097, 1098
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 2 cut(s) 1232, 1426
Bse1I ACTGG 3 cut(s) 623, 816, 1237
Bse21I CCTNAGG 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 2 cut(s) 1099, 1241
BseDI CCNNGG 5 cut(s) 372, 453, 546, 1097, 1098
BseGI GGATG 2 cut(s) 717, 996
BseLI CCNNNNNNNGG 2 cut(s) 1232, 1426
BseMII CTCAG 3 cut(s) 113, 508, 954
BseNI ACTGG 3 cut(s) 623, 816, 1237
BseRI GAGGAG 1 cut(s) 482
BseSI GKGCMC 1 cut(s) 1374
BseXI GCAGC 3 cut(s) 104, 254, 933
BseYI CCCAGC 4 cut(s) 117, 874, 1048, 1367
Bsh1236I CGCG 1 cut(s) 819
BshFI GGCC 3 cut(s) 14, 304, 716
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 5 cut(s) 126, 162, 264, 453, 546
BslFI GGGAC 4 cut(s) 557, 680, 771, 1080
BslI CCNNNNNNNGG 2 cut(s) 1232, 1426
BsmAI GTCTC 1 cut(s) 1200
BsmFI GGGAC 4 cut(s) 557, 680, 771, 1080
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 3 cut(s) 14, 304, 716
Bso31I GGTCTC 1 cut(s) 1200
BsoBI CYCGRG 1 cut(s) 515
Bsp1286I GDGCHC 3 cut(s) 107, 224, 1374
Bsp13I TCCGGA 1 cut(s) 161
Bsp143I GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
Bsp68I TCGCGA 1 cut(s) 819
BspACI CCGC 2 cut(s) 5, 225
BspANI GGCC 3 cut(s) 14, 304, 716
BspCNI CTCAG 3 cut(s) 112, 507, 955
BspEI TCCGGA 1 cut(s) 161
BspFNI CGCG 1 cut(s) 819
BspLI GGNNCC 3 cut(s) 221, 262, 1096
BspMI ACCTGC 2 cut(s) 47, 1304
BspPI GGATC 5 cut(s) 227, 465, 770, 1144, 1290
BspTI CTTAAG 2 cut(s) 64, 934
BspTNI GGTCTC 1 cut(s) 1200
BsrI ACTGG 3 cut(s) 623, 816, 1237
BssECI CCNNGG 5 cut(s) 372, 453, 546, 1097, 1098
BssMI GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
BssNAI GTATAC 2 cut(s) 605, 1230
BssSI CACGAG 1 cut(s) 1395
BssT1I CCWWGG 1 cut(s) 372
Bst1107I GTATAC 2 cut(s) 605, 1230
Bst2BI CACGAG 1 cut(s) 1395
Bst2UI CCWGG 2 cut(s) 1099, 1241
Bst4CI ACNGT 4 cut(s) 21, 390, 1357, 1378
Bst6I CTCTTC 1 cut(s) 980
BstAFI CTTAAG 2 cut(s) 64, 934
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstC8I GCNNGC 3 cut(s) 7, 1080, 1326
BstDEI CTNAG 3 cut(s) 99, 494, 963
BstF5I GGATG 2 cut(s) 717, 996
BstFNI CGCG 1 cut(s) 819
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 6 cut(s) 235, 460, 730, 765, 1152, 1298
BstMAI GTCTC 1 cut(s) 1200
BstMBI GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
BstMWI GCNNNNNNNGC 5 cut(s) 11, 135, 273, 1070, 1079
BstNI CCWGG 2 cut(s) 1099, 1241
BstNSI RCATGY 1 cut(s) 844
BstSCI CCNGG 6 cut(s) 124, 262, 452, 545, 1097, 1239
BstSFI CTRYAG 1 cut(s) 1414
BstSLI GKGCMC 1 cut(s) 1374
BstUI CGCG 1 cut(s) 819
BstV1I GCAGC 3 cut(s) 104, 254, 933
BstX2I RGATCY 1 cut(s) 457
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 1 cut(s) 457
BstZ17I GTATAC 2 cut(s) 605, 1230
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 3 cut(s) 14, 304, 716
BtgZI GCGATG 1 cut(s) 810
BtsCI GGATG 2 cut(s) 717, 996
BtsIMutI CAGTG 2 cut(s) 1207, 1353
BtuMI TCGCGA 1 cut(s) 819
BveI ACCTGC 2 cut(s) 47, 1304
Cac8I GCNNGC 3 cut(s) 7, 1080, 1326
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 260, 302, 369, 715, 1094
Csp6I GTAC 4 cut(s) 27, 511, 844, 851
CviAII CATG 9 cut(s) 10, 299, 646, 707, 841, 1008, 1070, 1211, 1245
CviQI GTAC 4 cut(s) 27, 511, 844, 851
DdeI CTNAG 3 cut(s) 99, 494, 963
DpnI GATC 6 cut(s) 234, 459, 729, 764, 1151, 1297
DpnII GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
DrdI GACNNNNNNGTC 1 cut(s) 1052
DseDI GACNNNNNNGTC 1 cut(s) 1052
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 980
EarI CTCTTC 1 cut(s) 980
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 107, 224
Eco31I GGTCTC 1 cut(s) 1200
Eco47I GGWCC 3 cut(s) 260, 369, 1094
Eco81I CCTNAGG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 1097, 1239
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 2 cut(s) 107, 224
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 9 cut(s) 13, 302, 649, 710, 844, 1011, 1073, 1214, 1248
FaqI GGGAC 4 cut(s) 557, 680, 771, 1080
FatI CATG 9 cut(s) 9, 298, 645, 706, 840, 1007, 1069, 1210, 1244
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 1330
FblI GTMKAC 4 cut(s) 291, 567, 604, 1229
Fnu4HI GCNGC 3 cut(s) 93, 268, 922
FokI GGATG 2 cut(s) 724, 1003
FriOI GRGCYC 2 cut(s) 107, 224
Fsp4HI GCNGC 3 cut(s) 93, 268, 922
FspBI CTAG 4 cut(s) 273, 783, 950, 1251
GlaI GCGC 1 cut(s) 181
GluI GCNGC 3 cut(s) 93, 268, 922
GsaI CCCAGC 4 cut(s) 121, 878, 1052, 1371
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 3 cut(s) 14, 304, 716
HapII CCGG 5 cut(s) 126, 162, 264, 453, 546
HhaI GCGC 1 cut(s) 182
Hin1II CATG 9 cut(s) 13, 302, 649, 710, 844, 1011, 1073, 1214, 1248
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HindIII AAGCTT 2 cut(s) 67, 937
HinfI GANTC 6 cut(s) 187, 285, 398, 551, 564, 660
HpaII CCGG 5 cut(s) 126, 162, 264, 453, 546
HphI GGTGA 2 cut(s) 106, 1336
Hpy166II GTNNAC 7 cut(s) 260, 292, 568, 605, 833, 1230, 1256
Hpy188I TCNGA 5 cut(s) 214, 475, 727, 964, 977
Hpy188III TCNNGA 5 cut(s) 162, 581, 818, 891, 1382
Hpy8I GTNNAC 7 cut(s) 260, 292, 568, 605, 833, 1230, 1256
HpyAV CCTTC 2 cut(s) 209, 496
HpyCH4III ACNGT 4 cut(s) 21, 390, 1357, 1378
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 4 cut(s) 1082, 1110, 1313, 1324
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 135, 273, 1070, 1079
HpyF3I CTNAG 3 cut(s) 99, 494, 963
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 9 cut(s) 13, 302, 649, 710, 844, 1011, 1073, 1214, 1248
HspAI GCGC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 161
Kzo9I GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
LmnI GCTCC 2 cut(s) 219, 1364
Lsp1109I GCAGC 3 cut(s) 104, 254, 933
MaeI CTAG 4 cut(s) 273, 783, 950, 1251
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 6 cut(s) 112, 448, 641, 863, 993, 1316
MalI GATC 6 cut(s) 234, 459, 729, 764, 1151, 1297
MboI GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
MboII GAAGA 3 cut(s) 452, 764, 997
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 3 cut(s) 107, 224, 1374
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 6 cut(s) 30, 84, 310, 1065, 1103, 1129
MluNI TGGCCA 1 cut(s) 14
MlyI GAGTC 2 cut(s) 560, 573
MmeI TCCRAC 3 cut(s) 405, 498, 798
Mox20I TGGCCA 1 cut(s) 14
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 3 cut(s) 191, 886, 1065
MscI TGGCCA 1 cut(s) 14
MseI TTAA 5 cut(s) 65, 354, 504, 935, 1014
Msp20I TGGCCA 1 cut(s) 14
MspA1I CMGCKG 3 cut(s) 366, 498, 878
MspCI CTTAAG 2 cut(s) 64, 934
MspI CCGG 5 cut(s) 126, 162, 264, 453, 546
MspR9I CCNGG 6 cut(s) 126, 264, 454, 547, 1099, 1241
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 2 cut(s) 1099, 1241
MvnI CGCG 1 cut(s) 819
MwoI GCNNNNNNNGC 5 cut(s) 11, 135, 273, 1070, 1079
NciI CCSGG 4 cut(s) 126, 264, 454, 547
NdeI CATATG 1 cut(s) 1330
NdeII GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
NlaIII CATG 9 cut(s) 13, 302, 649, 710, 844, 1011, 1073, 1214, 1248
NlaIV GGNNCC 3 cut(s) 221, 262, 1096
NmuCI GTSAC 2 cut(s) 112, 993
NruI TCGCGA 1 cut(s) 819
NspI RCATGY 1 cut(s) 844
PaqCI CACCTGC 1 cut(s) 47
PasI CCCWGGG 1 cut(s) 1098
PciI ACATGT 1 cut(s) 840
PctI GAATGC 1 cut(s) 335
PdmI GAANNNNTTC 3 cut(s) 191, 886, 1065
PfeI GAWTC 4 cut(s) 187, 285, 398, 660
PkrI GCNGC 3 cut(s) 94, 269, 923
PleI GAGTC 2 cut(s) 559, 572
PpsI GAGTC 2 cut(s) 559, 572
PscI ACATGT 1 cut(s) 840
PsiI TTATAA 2 cut(s) 1128, 1266
Psp6I CCWGG 2 cut(s) 1097, 1239
PspFI CCCAGC 4 cut(s) 117, 874, 1048, 1367
PspGI CCWGG 2 cut(s) 1097, 1239
PspN4I GGNNCC 3 cut(s) 221, 262, 1096
PspPI GGNCC 5 cut(s) 260, 302, 369, 715, 1094
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 3 cut(s) 366, 498, 878
RruI TCGCGA 1 cut(s) 819
RsaI GTAC 4 cut(s) 28, 512, 845, 852
RsaNI GTAC 4 cut(s) 27, 511, 844, 851
SaqAI TTAA 5 cut(s) 65, 354, 504, 935, 1014
SatI GCNGC 3 cut(s) 93, 268, 922
Sau3AI GATC 6 cut(s) 232, 457, 727, 762, 1149, 1295
Sau96I GGNCC 5 cut(s) 260, 302, 369, 715, 1094
SchI GAGTC 2 cut(s) 560, 573
ScrFI CCNGG 6 cut(s) 126, 264, 454, 547, 1099, 1241
SduI GDGCHC 3 cut(s) 107, 224, 1374
SfcI CTRYAG 1 cut(s) 1414
SinI GGWCC 3 cut(s) 260, 369, 1094
SmlI CTYRAG 4 cut(s) 64, 635, 854, 934
SmoI CTYRAG 4 cut(s) 64, 635, 854, 934
SpeI ACTAGT 1 cut(s) 1250
Sse9I AATT 6 cut(s) 30, 84, 310, 1065, 1103, 1129
SsiI CCGC 2 cut(s) 5, 225
SspMI CTAG 4 cut(s) 273, 783, 950, 1251
StyD4I CCNGG 6 cut(s) 124, 262, 452, 545, 1097, 1239
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 4 cut(s) 21, 390, 1357, 1378
TaiI ACGT 1 cut(s) 573
TaqI TCGA 1 cut(s) 1407
TasI AATT 6 cut(s) 30, 84, 310, 1065, 1103, 1129
TatI WGTACW 1 cut(s) 850
TfiI GAWTC 4 cut(s) 187, 285, 398, 660
Tru1I TTAA 5 cut(s) 65, 354, 504, 935, 1014
Tru9I TTAA 5 cut(s) 65, 354, 504, 935, 1014
TscAI CASTG 2 cut(s) 1207, 1360
TseFI GTSAC 2 cut(s) 112, 993
TseI GCWGC 3 cut(s) 92, 267, 921
Tsp45I GTSAC 2 cut(s) 112, 993
TspDTI ATGAA 3 cut(s) 1035, 1058, 1347
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 2 cut(s) 1207, 1360
Vha464I CTTAAG 2 cut(s) 64, 934
VpaK11BI GGWCC 3 cut(s) 260, 369, 1094
XceI RCATGY 1 cut(s) 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 875
XmiI GTMKAC 4 cut(s) 291, 567, 604, 1229
XmnI GAANNNNTTC 3 cut(s) 191, 886, 1065
XspI CTAG 4 cut(s) 273, 783, 950, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.