AT3G30220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Reverse (-)
11877866 .. 11878458
593 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G30220.1

Sequence Viewer

Length: 438 bp
ATGGAAAACGACGGAGCCTCCACCGTCTGTCCTCTCGACCTTGGCAGTCAAATGTTTTTATCCGGCGATGGTTTTGAACCGGCCAGTGTCCTCTTTCCCCACGCTGCTTTTCACTTTCCTCCAAAAGACAAAAAGGAGGCGTACGATTTCATGGACAAGCGTTTAGGCCTCAAGAGTTTGCCTCTTGAGGCCCGCATCGCTCGAGTGGAGAGGAAGGTGAGGGCCATGGAGAGTGATCATTTCCAATCGAATTGGAGGAAGGTGCGTGGCCAGGATCCCGACTATGCTTCGGATTTACCTCCATGCCCGTGGTCAAGTCCAAGCGAGGAGGACGACGAGGAAGAGGATAGCAACCACTGGAAGCGTGTCAAGAATGAACCCGAGGTGGATGATAAAAATGAAGCATCCACTTCGAACGCTGATCCGGCGATGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

16.43

Weight (kDa)

4.76

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3287 PF11690 3 - 84 6.7e-19 Protein of unknown function (DUF3287)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 193
AclWI GGATC 3 cut(s) 269, 282, 416
AcoI YGGCCR 2 cut(s) 81, 268
AfaI GTAC 1 cut(s) 143
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 1 cut(s) 270
AlwI GGATC 3 cut(s) 269, 282, 416
Ama87I CYCGRG 2 cut(s) 201, 380
AoxI GGCC 5 cut(s) 81, 166, 189, 222, 268
ApeKI GCWGC 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 190, 222
AsuHPI GGTGA 1 cut(s) 229
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 2 cut(s) 201, 380
BalI TGGCCA 1 cut(s) 270
BamHI GGATCC 1 cut(s) 274
BbvI GCAGC 1 cut(s) 91
BccI CCATC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 1 cut(s) 235
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 272
BmeT110I CYCGRG 2 cut(s) 201, 380
BmgT120I GGNCC 2 cut(s) 190, 222
BmiI GGNNCC 2 cut(s) 16, 276
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 204, 413
BplI GAGNNNNNCTC 2 cut(s) 166, 198
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 155, 206
BsaJI CCNNGG 4 cut(s) 40, 225, 308, 381
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 84, 362
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 4 cut(s) 40, 225, 308, 381
BseGI GGATG 2 cut(s) 394, 404
BseNI ACTGG 2 cut(s) 84, 362
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 91
BshFI GGCC 5 cut(s) 83, 168, 191, 224, 270
BsiHKCI CYCGRG 2 cut(s) 201, 380
BsiSI CCGG 3 cut(s) 63, 80, 425
BsiWI CGTACG 1 cut(s) 141
BsnI GGCC 5 cut(s) 83, 168, 191, 224, 270
BsoBI CYCGRG 2 cut(s) 201, 380
Bsp119I TTCGAA 1 cut(s) 413
Bsp143I GATC 3 cut(s) 235, 274, 421
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 1 cut(s) 193
BspANI GGCC 5 cut(s) 83, 168, 191, 224, 270
BspLI GGNNCC 2 cut(s) 16, 276
BspPI GGATC 3 cut(s) 269, 282, 416
BspT104I TTCGAA 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 84, 362
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 4 cut(s) 40, 225, 308, 381
BssMI GATC 3 cut(s) 235, 274, 421
BssT1I CCWWGG 2 cut(s) 40, 225
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 193
BstDSI CCRYGG 2 cut(s) 225, 308
BstF5I GGATG 2 cut(s) 394, 404
BstKTI GATC 3 cut(s) 238, 277, 424
BstMBI GATC 3 cut(s) 235, 274, 421
BstMWI GCNNNNNNNGC 2 cut(s) 197, 425
BstNI CCWGG 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 274
BstXI CCANNNNNNTGG 1 cut(s) 309
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 5 cut(s) 83, 168, 191, 224, 270
BtgI CCRYGG 2 cut(s) 225, 308
BtgZI GCGATG 2 cut(s) 81, 181
BtsCI GGATG 2 cut(s) 394, 404
BtsIMutI CAGTG 2 cut(s) 91, 355
Cac8I GCNNGC 1 cut(s) 193
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 190, 222
Csp6I GTAC 1 cut(s) 142
CviAII CATG 3 cut(s) 151, 226, 303
CviJI RGCY 6 cut(s) 17, 83, 168, 191, 224, 270
CviKI_1 RGCY 6 cut(s) 17, 83, 168, 191, 224, 270
CviQI GTAC 1 cut(s) 142
DpnI GATC 3 cut(s) 237, 276, 423
DpnII GATC 3 cut(s) 235, 274, 421
EaeI YGGCCR 2 cut(s) 81, 268
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 40, 225
Eco147I AGGCCT 1 cut(s) 168
Eco88I CYCGRG 2 cut(s) 201, 380
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 40, 225
ErhI CCWWGG 2 cut(s) 40, 225
FaeI CATG 3 cut(s) 154, 229, 306
FaiI YATR 4 cut(s) 152, 227, 285, 304
FatI CATG 3 cut(s) 150, 225, 302
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 2 cut(s) 391, 401
Fsp4HI GCNGC 1 cut(s) 105
GluI GCNGC 1 cut(s) 105
HaeIII GGCC 5 cut(s) 83, 168, 191, 224, 270
HapII CCGG 3 cut(s) 63, 80, 425
Hin1II CATG 3 cut(s) 154, 229, 306
HpaII CCGG 3 cut(s) 63, 80, 425
HphI GGTGA 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 5 cut(s) 35, 172, 185, 278, 370
Hpy99I CGWCG 2 cut(s) 14, 338
HpyAV CCTTC 2 cut(s) 208, 253
HpyCH4III ACNGT 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 425
Hsp92II CATG 3 cut(s) 154, 229, 306
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 235, 274, 421
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 6 cut(s) 76, 93, 97, 257, 284, 343
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 2 cut(s) 204, 413
MalI GATC 3 cut(s) 237, 276, 423
MboI GATC 3 cut(s) 235, 274, 421
MboII GAAGA 1 cut(s) 353
MflI RGATCY 1 cut(s) 274
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 1 cut(s) 250
MluNI TGGCCA 1 cut(s) 270
Mox20I TGGCCA 1 cut(s) 270
MscI TGGCCA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 307
Msp20I TGGCCA 1 cut(s) 270
MspI CCGG 3 cut(s) 63, 80, 425
MspR9I CCNGG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 197, 425
NcoI CCATGG 1 cut(s) 225
NdeII GATC 3 cut(s) 235, 274, 421
NlaIII CATG 3 cut(s) 154, 229, 306
NlaIV GGNNCC 2 cut(s) 16, 276
NspV TTCGAA 1 cut(s) 413
PaeR7I CTCGAG 1 cut(s) 201
PceI AGGCCT 1 cut(s) 168
Pfl23II CGTACG 1 cut(s) 141
PkrI GCNGC 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 16, 276
PspPI GGNCC 2 cut(s) 190, 222
PspXI VCTCGAGB 1 cut(s) 201
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 307
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 3 cut(s) 235, 274, 421
Sau96I GGNCC 2 cut(s) 190, 222
ScrFI CCNGG 1 cut(s) 272
SetI ASST 5 cut(s) 42, 219, 264, 301, 387
SfaNI GCATC 2 cut(s) 204, 413
Sfr274I CTCGAG 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 413
SlaI CTCGAG 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 307
SmlI CTYRAG 3 cut(s) 170, 185, 201
SmoI CTYRAG 3 cut(s) 170, 185, 201
Sse9I AATT 1 cut(s) 250
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 1 cut(s) 193
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 2 cut(s) 40, 225
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 4 cut(s) 36, 202, 248, 413
TasI AATT 1 cut(s) 250
TscAI CASTG 2 cut(s) 91, 362
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 3 cut(s) 139, 390, 414
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 2 cut(s) 91, 362
XhoI CTCGAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.