AT3G32150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Forward (+)
13143627 .. 13144825
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G32150.1

Sequence Viewer

Length: 726 bp
ATGTCTTCCGATGCTTCAAGACTCGGAAAGACACAATCCTGCCAACCTTTGATAGGTGGCGCCCTGCAATCCGTGAACACTTTTTACTTCACGCTCACTACTCGTCCCAGGTACGACAAGCTTCAAGGTGAGCTCTCGACTACTCAAGACCGGCTCTCGGAATCCGAATCGGCCGCCTATGGCTTGAACGACCAGTTCTCCGAACTCAAGGCGAAGTACAATGCAATCACCGAGAAAACTAGGCCGGAGATGGAATCGGATATCAAAGAGTATGAAAGCAACCTTCTCCTTCTGGATCAAACCCACGACGAAGACTTTTCTGAGGAACAAGAAAGGTCCGTATTCGAAGCTGTTTTAGTAGAGAAGCGAAGTCGTCTGGCTGCTCTTACCTCTTCATCATTCAACCCTCAACAGTTCGAGGAGTTCTTTACCGAATTGCCTCCTTTGAGCGAGTCCGGTTTAGATTGGGCTGGTCCCAGTGAACCCGTGGAACCTGAGATATCTGTCACGTCGGTGGAAACTCCTGAACCGGTAGTCATTCCGCCAGAGGTTCCTCCATCCACGAACACCGAAACAGTGGTCATCGAGGATGATGATGCTTCAGACCTAGGGATTCCCACCGAACAACCTGTCGCTGATGAAACAAACGAGACTGAGACGACTGCCATCGATTCGGTCGATCCTGAGCCAACTCAAACTGAATCCAAGGTGGATAAGGATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

26.67

Weight (kDa)

4.05

Isoelectric Point (pI)

64.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AciI CCGC 2 cut(s) 174, 542
AclWI GGATC 3 cut(s) 303, 674, 713
AcoI YGGCCR 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 2 cut(s) 113, 218
AfiI CCNNNNNNNGG 2 cut(s) 53, 157
AgeI ACCGGT 1 cut(s) 529
AgsI TTSAA 4 cut(s) 18, 125, 187, 403
AjiI CACGTC 1 cut(s) 510
AjnI CCWGG 1 cut(s) 107
AleI CACNNNNGTG 1 cut(s) 512
AluBI AGCT 3 cut(s) 121, 133, 350
AluI AGCT 3 cut(s) 121, 133, 350
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 644, 650
AlwI GGATC 3 cut(s) 303, 674, 713
AoxI GGCC 2 cut(s) 171, 242
ApeKI GCWGC 1 cut(s) 380
ArsI GACNNNNNNTTYG 4 cut(s) 564, 596, 615, 647
AsiGI ACCGGT 1 cut(s) 529
AspA2I CCTAGG 1 cut(s) 607
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 336, 473
AsuHPI GGTGA 2 cut(s) 140, 220
AsuII TTCGAA 1 cut(s) 345
AvaII GGWCC 2 cut(s) 336, 473
AvrII CCTAGG 1 cut(s) 607
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 367
BccI CCATC 3 cut(s) 244, 565, 674
BciT130I CCWGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 644, 650
BfaI CTAG 2 cut(s) 240, 608
BfoI RGCGCY 1 cut(s) 63
BisI GCNGC 2 cut(s) 174, 381
BlnI CCTAGG 1 cut(s) 607
BlsI GCNGC 2 cut(s) 175, 382
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 2 cut(s) 336, 473
BmgBI CACGTC 1 cut(s) 510
BmgT120I GGNCC 2 cut(s) 336, 473
BmiI GGNNCC 5 cut(s) 61, 475, 492, 552, 720
BmrFI CCNGG 1 cut(s) 109
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 1 cut(s) 586
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 684
Bpu14I TTCGAA 1 cut(s) 345
BpuEI CTTGAG 2 cut(s) 129, 191
Bsa29I ATCGAT 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 4 cut(s) 107, 486, 607, 705
BsaWI WCCGGW 2 cut(s) 455, 529
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 157
Bse118I RCCGGY 2 cut(s) 150, 529
Bse1I ACTGG 2 cut(s) 193, 477
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 1 cut(s) 109
BseCI ATCGAT 1 cut(s) 669
BseDI CCNNGG 4 cut(s) 107, 486, 607, 705
BseGI GGATG 2 cut(s) 557, 595
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 2 cut(s) 53, 157
BseMII CTCAG 4 cut(s) 312, 486, 645, 675
BseNI ACTGG 2 cut(s) 193, 477
BseRI GAGGAG 1 cut(s) 434
BseX3I CGGCCG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 367
Bsh1285I CGRYCG 2 cut(s) 174, 678
BshFI GGCC 2 cut(s) 173, 244
BshNI GGYRCC 1 cut(s) 59
BshTI ACCGGT 1 cut(s) 529
BshVI ATCGAT 1 cut(s) 669
BsiEI CGRYCG 2 cut(s) 174, 678
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 4 cut(s) 151, 245, 456, 530
BslFI GGGAC 2 cut(s) 90, 459
BslI CCNNNNNNNGG 2 cut(s) 53, 157
BsmAI GTCTC 2 cut(s) 644, 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 90, 459
BsnI GGCC 2 cut(s) 173, 244
Bsp119I TTCGAA 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 3 cut(s) 295, 679, 718
BspACI CCGC 2 cut(s) 174, 542
BspANI GGCC 2 cut(s) 173, 244
BspCNI CTCAG 4 cut(s) 313, 487, 646, 676
BspDI ATCGAT 1 cut(s) 669
BspLI GGNNCC 5 cut(s) 61, 475, 492, 552, 720
BspPI GGATC 3 cut(s) 303, 674, 713
BspT104I TTCGAA 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 59
BsrFI RCCGGY 2 cut(s) 150, 529
BsrI ACTGG 2 cut(s) 193, 477
BssAI RCCGGY 2 cut(s) 150, 529
BssECI CCNNGG 4 cut(s) 107, 486, 607, 705
BssMI GATC 3 cut(s) 295, 679, 718
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 2 cut(s) 607, 705
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 2 cut(s) 414, 577
Bst6I CTCTTC 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 345
BstDEI CTNAG 4 cut(s) 321, 495, 654, 684
BstDSI CCRYGG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 557, 595
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 3 cut(s) 298, 682, 721
BstMAI GTCTC 2 cut(s) 644, 650
BstMBI GATC 3 cut(s) 295, 679, 718
BstMCI CGRYCG 2 cut(s) 174, 678
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 367
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 669
BsuRI GGCC 2 cut(s) 173, 244
BsuTUI ATCGAT 1 cut(s) 669
BtgI CCRYGG 1 cut(s) 486
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 2 cut(s) 557, 595
BtsIMutI CAGTG 2 cut(s) 484, 582
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 2 cut(s) 150, 529
Cfr13I GGNCC 2 cut(s) 336, 473
ClaI ATCGAT 1 cut(s) 669
Csp6I GTAC 2 cut(s) 112, 217
CspAI ACCGGT 1 cut(s) 529
CviQI GTAC 2 cut(s) 112, 217
DdeI CTNAG 4 cut(s) 321, 495, 654, 684
DinI GGCGCC 1 cut(s) 61
DpnI GATC 3 cut(s) 297, 681, 720
DpnII GATC 3 cut(s) 295, 679, 718
EaeI YGGCCR 1 cut(s) 171
EagI CGGCCG 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 397
EciI GGCGGA 1 cut(s) 531
Ecl136II GAGCTC 1 cut(s) 133
EclXI CGGCCG 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 607, 705
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 2 cut(s) 262, 501
Eco47I GGWCC 2 cut(s) 336, 473
Eco52I CGGCCG 1 cut(s) 171
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 107
EcoRV GATATC 2 cut(s) 262, 501
EcoT14I CCWWGG 2 cut(s) 607, 705
EcoT38I GRGCYC 1 cut(s) 135
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 607, 705
Esp3I CGTCTC 1 cut(s) 650
FaiI YATR 3 cut(s) 180, 273, 724
FaqI GGGAC 2 cut(s) 90, 459
Fnu4HI GCNGC 2 cut(s) 174, 381
FokI GGATG 2 cut(s) 544, 602
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 174, 381
FspBI CTAG 2 cut(s) 240, 608
GlaI GCGC 1 cut(s) 61
GluI GCNGC 2 cut(s) 174, 381
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 2 cut(s) 173, 244
HapII CCGG 4 cut(s) 151, 245, 456, 530
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 60
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 8 cut(s) 21, 161, 167, 254, 452, 613, 671, 701
HpaII CCGG 4 cut(s) 151, 245, 456, 530
HphI GGTGA 2 cut(s) 140, 220
Hpy166II GTNNAC 2 cut(s) 76, 482
Hpy188I TCNGA 8 cut(s) 10, 26, 160, 166, 202, 259, 322, 604
Hpy188III TCNNGA 6 cut(s) 18, 136, 146, 293, 524, 683
Hpy8I GTNNAC 2 cut(s) 76, 482
Hpy99I CGWCG 2 cut(s) 311, 514
HpyAV CCTTC 2 cut(s) 293, 299
HpyCH4III ACNGT 2 cut(s) 414, 577
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 2 cut(s) 67, 224
HpyF3I CTNAG 4 cut(s) 321, 495, 654, 684
HpySE526I ACGT 1 cut(s) 509
Hsp92I GRCGYC 1 cut(s) 60
HspAI GCGC 1 cut(s) 60
KasI GGCGCC 1 cut(s) 59
Kzo9I GATC 3 cut(s) 295, 679, 718
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 1 cut(s) 586
MaeI CTAG 2 cut(s) 240, 608
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 1 cut(s) 505
MalI GATC 3 cut(s) 297, 681, 720
MboI GATC 3 cut(s) 295, 679, 718
MboII GAAGA 2 cut(s) 323, 384
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 1 cut(s) 434
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 2 cut(s) 15, 461
MnlI CCTC 8 cut(s) 316, 400, 412, 417, 450, 541, 564, 580
MslI CAYNNNNRTG 1 cut(s) 512
MspI CCGG 4 cut(s) 151, 245, 456, 530
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
NarI GGCGCC 1 cut(s) 60
NdeII GATC 3 cut(s) 295, 679, 718
NlaIV GGNNCC 5 cut(s) 61, 475, 492, 552, 720
NmuCI GTSAC 1 cut(s) 505
NspV TTCGAA 1 cut(s) 345
OliI CACNNNNGTG 1 cut(s) 512
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PfeI GAWTC 6 cut(s) 161, 167, 254, 613, 671, 701
PinAI ACCGGT 1 cut(s) 529
PkrI GCNGC 2 cut(s) 175, 382
PleI GAGTC 2 cut(s) 15, 460
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 2 cut(s) 15, 460
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 5 cut(s) 61, 475, 492, 552, 720
PspPI GGNCC 2 cut(s) 336, 473
PsrI GAACNNNNNNTAC 2 cut(s) 68, 100
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 2 cut(s) 113, 218
RsaNI GTAC 2 cut(s) 112, 217
RseI CAYNNNNRTG 1 cut(s) 512
SacI GAGCTC 1 cut(s) 135
SatI GCNGC 2 cut(s) 174, 381
Sau3AI GATC 3 cut(s) 295, 679, 718
Sau96I GGNCC 2 cut(s) 336, 473
SchI GAGTC 2 cut(s) 15, 461
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 1 cut(s) 586
SfoI GGCGCC 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 345
SinI GGWCC 2 cut(s) 336, 473
SmiMI CAYNNNNRTG 1 cut(s) 512
SmlI CTYRAG 2 cut(s) 144, 206
SmoI CTYRAG 2 cut(s) 144, 206
Sse9I AATT 1 cut(s) 434
SsiI CCGC 2 cut(s) 174, 542
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 2 cut(s) 240, 608
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 2 cut(s) 607, 705
TaaI ACNGT 2 cut(s) 414, 577
TaiI ACGT 1 cut(s) 512
TaqI TCGA 6 cut(s) 137, 345, 417, 585, 669, 678
TaqII GACCGA 1 cut(s) 664
TasI AATT 1 cut(s) 434
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 6 cut(s) 161, 167, 254, 613, 671, 701
TscAI CASTG 2 cut(s) 484, 582
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 288, 384, 654
TspGWI ACGGA 2 cut(s) 61, 328
TspRI CASTG 2 cut(s) 484, 582
VpaK11BI GGWCC 2 cut(s) 336, 473
XagI CCTNNNNNAGG 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmaJI CCTAGG 1 cut(s) 607
XspI CTAG 2 cut(s) 240, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.