AT3G30230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Reverse (-)
11879235 .. 11881788
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G30230.1

Sequence Viewer

Length: 1584 bp
ATGAATCCTGATACTGAAAGACCCGAATCCCCGTCTTTCGACGAGGGGGGTTCTAACAATGACATATGCACTCGCCTGAACGATATCGCCGGTCCACACTACACATCCATCTGTACCCTTCAATCCCTCGAACGACTAAAAGATCTCTACCAGATTCTGCCTGAGATTATGGCGGAATCAGAGATGCATGGCCCCTTTGAGTCCCCGGAAGACCCTCGCCCTGGTTATTTATGCGTTTACAAGATCTATTTCAAGGGCTGCGGGTTGACCTTCCCTCTTCCCAAGGCCTTGGTCTGTTACTTAGATGCTCTTATAATTGCTCTACCCCAACTAACCCCCAACCTCCTCCGAACTATCTTTGGGATCATAACTATTGCGGCGGAAGCCGGATTCGTTATCGGGGTCCCTGAACTGAACGAGCTTCTCACCATGAGAAGTTCATCAAAGAAAAATGGATCAACTCCATGTCCTGAACGTCGTGGTGGCTGTTCGCCTCGGAGAAACTTATCTCCCCATCGCAACTCTCCTCGTTACTCTCCCCGAAATAGACTGAGTAGCAAACCAGTTGCTGACTTAATTCGCAAGAAGAGAGACAGATCAGCCCGAGGTAACTCTTCCCCACGGAGAGAGAAATCAAAGGCTCGGACAGACCGTTCCCCTAGGTTATCTCTTCCACCTCGATCGATGGGTCCTCCCCTTCCTGTGGTCACATCTCCCCCTCCTCGACTTAGTGGCGAGAAGACTGACCGAGGTGTGACTCCTCGACAGGAAGCTATGGAAACAGAGGGTAACGTTTTCCGATGTTTCAAGACCCAAAAGGACGCGATCTTGCCGAACTTTGATAGATGGCGTCCTACAATCCACAAACGCTTTTTACTTCATGCCTACCATTCATCTCAGGGCAAGTTTTCTTCCCTTGAGGCTGACCTCCGCTCCCTTTCTGATTCCAAACAAAAGTTGGAGGATCAAGTTGACCTTTTCTCTACCGAACTCAAGAAATCAAACGCCGAATTGCAAGATCAGTATCGCAGACATGACAAACTCCAAGATGAACTCTCAGTTGCCCGAGGCAGGCTTTCTGAATCCAAATCGGCCGCCTACACCTTGAACAACCAGTTCACCAAACTCGAGGCGAAGTACAAAGCCATCACCAAACTTCGAGATGCTGAGTTGGCTAAGTCAGCGGCTAAAGCCAGAAATGAGGTGAAGGGTCGCGGGATGGAGTTGATTCAAGGGGCCATTCTTTTCATTCAAACCGAGCAGGCACGTTCAGAACTAGAATCTGATATCAAGGAGCATGAAAGCAACCTACTCTTACTAGACCAAATCCACAAAGATGATTTCTCTGAGGAGCAAGAAAGGTCCGATTTGAAAGCCGTTCTATACGAGAAACGTATTCGCCTTGCTGCTCTTCCCGCTTCATCCTTCAACCCTCAGCACTTCGAGGAGTTCTTTACCCAATCACCCCCCTTGGGTGAATCGGGTTTAAATTGGGCTGGATCAAGTGAGCCCGTTGATACTGCCGCACCCGAGGTGCCTGCTACATCGATAGGGATTGTTGAACCTGATCCTGCTTTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

527

Amino Acids

59.06

Weight (kDa)

8.6

Isoelectric Point (pI)

60.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 1534
AccBSI CCGCTC 1 cut(s) 933
AccII CGCG 2 cut(s) 824, 1215
AclI AACGTT 1 cut(s) 792
AclWI GGATC 5 cut(s) 371, 463, 972, 1507, 1562
AcoI YGGCCR 1 cut(s) 1092
AcyI GRCGYC 1 cut(s) 850
AfaI GTAC 2 cut(s) 115, 1139
AfiI CCNNNNNNNGG 4 cut(s) 221, 703, 1071, 1472
AgsI TTSAA 9 cut(s) 122, 253, 808, 1108, 1232, 1253, 1372, 1429, 1562
AjnI CCWGG 1 cut(s) 220
AloI GAACNNNNNNTCC 2 cut(s) 637, 669
AluBI AGCT 2 cut(s) 421, 773
AluI AGCT 2 cut(s) 421, 773
Alw26I GTCTC 1 cut(s) 585
AlwI GGATC 5 cut(s) 371, 463, 972, 1507, 1562
AlwNI CAGNNNCTG 2 cut(s) 157, 569
Ama87I CYCGRG 4 cut(s) 603, 1065, 1127, 1529
AoxI GGCC 4 cut(s) 190, 285, 1092, 1236
ApeKI GCWGC 2 cut(s) 258, 1406
AspA2I CCTAGG 1 cut(s) 659
AspS9I GGNCC 6 cut(s) 92, 191, 403, 689, 1236, 1362
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 6 cut(s) 418, 1111, 1141, 1216, 1455, 1487
AvaI CYCGRG 4 cut(s) 603, 1065, 1127, 1529
AvaII GGWCC 4 cut(s) 92, 403, 689, 1362
AvrII CCTAGG 1 cut(s) 659
BanI GGYRCC 1 cut(s) 1534
BanII GRGCYC 1 cut(s) 1512
BbsI GAAGAC 2 cut(s) 216, 746
BbvCI CCTCAGC 1 cut(s) 1434
BbvI GCAGC 2 cut(s) 245, 1393
BccI CCATC 6 cut(s) 116, 522, 679, 840, 1154, 1213
BceAI ACGGC 1 cut(s) 1361
BcgI CGANNNNNNTGC 2 cut(s) 1520, 1554
BciT130I CCWGG 1 cut(s) 222
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 3 cut(s) 660, 1277, 1319
BglII AGATCT 2 cut(s) 142, 243
BisI GCNGC 6 cut(s) 259, 378, 1095, 1185, 1407, 1524
BlnI CCTAGG 1 cut(s) 659
BlsI GCNGC 6 cut(s) 260, 379, 1096, 1186, 1408, 1525
Bme1390I CCNGG 2 cut(s) 206, 222
Bme18I GGWCC 4 cut(s) 92, 403, 689, 1362
BmeT110I CYCGRG 4 cut(s) 603, 1065, 1127, 1529
BmgT120I GGNCC 6 cut(s) 92, 191, 403, 689, 1236, 1362
BmiI GGNNCC 6 cut(s) 193, 404, 405, 690, 1237, 1536
BmrFI CCNGG 2 cut(s) 206, 222
BmsI GCATC 3 cut(s) 174, 295, 1153
BpiI GAAGAC 2 cut(s) 216, 746
Bpu10I CCTNAGC 1 cut(s) 1434
BpuEI CTTGAG 2 cut(s) 938, 977
BpuMI CCSGG 1 cut(s) 206
Bsa29I ATCGAT 2 cut(s) 683, 1547
BsaBI GATNNNNATC 1 cut(s) 1023
BsaHI GRCGYC 1 cut(s) 850
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 703, 1071, 1472
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 563, 1114
Bse8I GATNNNNATC 1 cut(s) 1023
BseBI CCWGG 1 cut(s) 222
BseCI ATCGAT 2 cut(s) 683, 1547
BseGI GGATG 3 cut(s) 104, 1224, 1421
BseJI GATNNNNATC 1 cut(s) 1023
BseLI CCNNNNNNNGG 4 cut(s) 221, 703, 1071, 1472
BseMII CTCAG 7 cut(s) 153, 542, 911, 1071, 1158, 1338, 1448
BseNI ACTGG 2 cut(s) 563, 1114
BseRI GAGGAG 6 cut(s) 335, 516, 711, 750, 1364, 1460
BseX3I CGGCCG 1 cut(s) 1092
BseXI GCAGC 2 cut(s) 245, 1393
Bsh1236I CGCG 2 cut(s) 824, 1215
Bsh1285I CGRYCG 2 cut(s) 683, 1095
BshFI GGCC 4 cut(s) 192, 287, 1094, 1238
BshNI GGYRCC 1 cut(s) 1534
BshVI ATCGAT 2 cut(s) 683, 1547
BsiEI CGRYCG 2 cut(s) 683, 1095
BsiHKCI CYCGRG 4 cut(s) 603, 1065, 1127, 1529
BsiSI CCGG 3 cut(s) 90, 206, 387
BslFI GGGAC 2 cut(s) 187, 389
BslI CCNNNNNNNGG 4 cut(s) 221, 703, 1071, 1472
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 2 cut(s) 187, 389
BsnI GGCC 4 cut(s) 192, 287, 1094, 1238
BsoBI CYCGRG 4 cut(s) 603, 1065, 1127, 1529
Bsp1286I GDGCHC 1 cut(s) 1512
BspANI GGCC 4 cut(s) 192, 287, 1094, 1238
BspCNI CTCAG 7 cut(s) 154, 543, 910, 1070, 1159, 1339, 1447
BspDI ATCGAT 2 cut(s) 683, 1547
BspFNI CGCG 2 cut(s) 824, 1215
BspLI GGNNCC 6 cut(s) 193, 404, 405, 690, 1237, 1536
BspPI GGATC 5 cut(s) 371, 463, 972, 1507, 1562
BspQI GCTCTTC 1 cut(s) 1416
BspT107I GGYRCC 1 cut(s) 1534
BsrBI CCGCTC 1 cut(s) 933
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 2 cut(s) 563, 1114
BssAI RCCGGY 1 cut(s) 89
BssNI GRCGYC 1 cut(s) 850
BssT1I CCWWGG 4 cut(s) 282, 288, 659, 1470
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 653
Bst6I CTCTTC 5 cut(s) 282, 581, 619, 675, 1416
BstACI GRCGYC 1 cut(s) 850
BstC8I GCNNGC 3 cut(s) 1073, 1263, 1539
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 3 cut(s) 104, 1224, 1421
BstFNI CGCG 2 cut(s) 824, 1215
BstMAI GTCTC 1 cut(s) 585
BstMCI CGRYCG 2 cut(s) 683, 1095
BstMWI GCNNNNNNNGC 5 cut(s) 383, 1172, 1181, 1190, 1415
BstNI CCWGG 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 204, 220
BstUI CGCG 2 cut(s) 824, 1215
BstV1I GCAGC 2 cut(s) 245, 1393
BstV2I GAAGAC 2 cut(s) 216, 746
BstX2I RGATCY 2 cut(s) 142, 243
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 2 cut(s) 142, 243
BstZI CGGCCG 1 cut(s) 1092
Bsu15I ATCGAT 2 cut(s) 683, 1547
BsuRI GGCC 4 cut(s) 192, 287, 1094, 1238
BsuTUI ATCGAT 2 cut(s) 683, 1547
BtgI CCRYGG 1 cut(s) 620
BtgZI GCGATG 1 cut(s) 500
BtsCI GGATG 3 cut(s) 104, 1224, 1421
Cac8I GCNNGC 3 cut(s) 1073, 1263, 1539
CaiI CAGNNNCTG 2 cut(s) 157, 569
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 6 cut(s) 92, 191, 403, 689, 1236, 1362
ClaI ATCGAT 2 cut(s) 683, 1547
CseI GACGC 2 cut(s) 830, 839
Csp6I GTAC 2 cut(s) 114, 1138
CviAII CATG 6 cut(s) 188, 430, 465, 881, 1034, 1298
CviQI GTAC 2 cut(s) 114, 1138
DraI TTTAAA 1 cut(s) 1488
EaeI YGGCCR 1 cut(s) 1092
EagI CGGCCG 1 cut(s) 1092
Eam1104I CTCTTC 5 cut(s) 282, 581, 619, 675, 1416
EarI CTCTTC 5 cut(s) 282, 581, 619, 675, 1416
EciI GGCGGA 2 cut(s) 188, 395
EclXI CGGCCG 1 cut(s) 1092
Eco130I CCWWGG 4 cut(s) 282, 288, 659, 1470
Eco147I AGGCCT 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 1512
Eco32I GATATC 2 cut(s) 85, 1288
Eco47I GGWCC 4 cut(s) 92, 403, 689, 1362
Eco52I CGGCCG 1 cut(s) 1092
Eco88I CYCGRG 4 cut(s) 603, 1065, 1127, 1529
EcoO109I RGGNCCY 2 cut(s) 403, 689
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 2 cut(s) 85, 1288
EcoT14I CCWWGG 4 cut(s) 282, 288, 659, 1470
EcoT22I ATGCAT 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 1512
ErhI CCWWGG 4 cut(s) 282, 288, 659, 1470
FaeI CATG 6 cut(s) 191, 433, 468, 884, 1037, 1301
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 2 cut(s) 187, 389
FatI CATG 6 cut(s) 187, 429, 464, 880, 1033, 1297
FauI CCCGC 3 cut(s) 254, 1208, 1423
FauNDI CATATG 1 cut(s) 65
Fnu4HI GCNGC 6 cut(s) 259, 378, 1095, 1185, 1407, 1524
FokI GGATG 3 cut(s) 91, 1231, 1408
FriOI GRGCYC 1 cut(s) 1512
Fsp4HI GCNGC 6 cut(s) 259, 378, 1095, 1185, 1407, 1524
FspBI CTAG 3 cut(s) 660, 1277, 1319
GluI GCNGC 6 cut(s) 259, 378, 1095, 1185, 1407, 1524
HaeIII GGCC 4 cut(s) 192, 287, 1094, 1238
HapII CCGG 3 cut(s) 90, 206, 387
HgaI GACGC 2 cut(s) 830, 839
Hin1I GRCGYC 1 cut(s) 850
Hin1II CATG 6 cut(s) 191, 433, 468, 884, 1037, 1301
HincII GTYRAC 2 cut(s) 267, 973
HindII GTYRAC 2 cut(s) 267, 973
HpaII CCGG 3 cut(s) 90, 206, 387
HphI GGTGA 6 cut(s) 418, 1111, 1141, 1216, 1455, 1487
Hpy166II GTNNAC 5 cut(s) 95, 238, 267, 973, 1119
Hpy188III TCNNGA 5 cut(s) 8, 470, 808, 994, 1160
Hpy8I GTNNAC 5 cut(s) 95, 238, 267, 973, 1119
Hpy99I CGWCG 2 cut(s) 44, 480
HpyAV CCTTC 5 cut(s) 128, 280, 707, 1201, 1435
HpyCH4III ACNGT 1 cut(s) 653
HpyCH4IV ACGT 4 cut(s) 475, 792, 1267, 1393
HpyCH4V TGCA 3 cut(s) 69, 187, 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 1172, 1181, 1190, 1415
HpySE526I ACGT 4 cut(s) 475, 792, 1267, 1393
Hsp92I GRCGYC 1 cut(s) 850
Hsp92II CATG 6 cut(s) 191, 433, 468, 884, 1037, 1301
KflI GGGWCCC 1 cut(s) 403
LguI GCTCTTC 1 cut(s) 1416
LmnI GCTCC 3 cut(s) 938, 1294, 1351
Lsp1109I GCAGC 2 cut(s) 245, 1393
LweI GCATC 3 cut(s) 174, 295, 1153
MaeI CTAG 3 cut(s) 660, 1277, 1319
MaeII ACGT 4 cut(s) 475, 792, 1267, 1393
MaeIII GTNAC 6 cut(s) 296, 530, 608, 706, 754, 788
MbiI CCGCTC 1 cut(s) 933
MboII GAAGA 8 cut(s) 221, 269, 598, 606, 662, 751, 903, 1403
MflI RGATCY 2 cut(s) 142, 243
MhlI GDGCHC 1 cut(s) 1512
MluCI AATT 4 cut(s) 315, 576, 1010, 1489
MlyI GAGTC 2 cut(s) 209, 751
MmeI TCCRAC 1 cut(s) 939
Mph1103I ATGCAT 1 cut(s) 189
MseI TTAA 3 cut(s) 575, 1487, 1582
MslI CAYNNNNRTG 1 cut(s) 1335
MspA1I CMGCKG 1 cut(s) 1184
MspI CCGG 3 cut(s) 90, 206, 387
MspR9I CCNGG 2 cut(s) 206, 222
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 2 cut(s) 824, 1215
MwoI GCNNNNNNNGC 5 cut(s) 383, 1172, 1181, 1190, 1415
NciI CCSGG 1 cut(s) 206
NdeI CATATG 1 cut(s) 65
NlaIII CATG 6 cut(s) 191, 433, 468, 884, 1037, 1301
NlaIV GGNNCC 6 cut(s) 193, 404, 405, 690, 1237, 1536
NmuCI GTSAC 2 cut(s) 706, 754
NsiI ATGCAT 1 cut(s) 189
PaeR7I CTCGAG 1 cut(s) 1127
PceI AGGCCT 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 1 cut(s) 649
PkrI GCNGC 6 cut(s) 260, 379, 1096, 1186, 1408, 1525
Ple19I CGATCG 1 cut(s) 683
PleI GAGTC 2 cut(s) 208, 751
PpsI GAGTC 2 cut(s) 208, 751
PpuMI RGGWCCY 2 cut(s) 403, 689
PsiI TTATAA 1 cut(s) 314
Psp1406I AACGTT 1 cut(s) 792
Psp5II RGGWCCY 2 cut(s) 403, 689
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 6 cut(s) 193, 404, 405, 690, 1237, 1536
PspPI GGNCC 6 cut(s) 92, 191, 403, 689, 1236, 1362
PspPPI RGGWCCY 2 cut(s) 403, 689
PspXI VCTCGAGB 1 cut(s) 1127
PstNI CAGNNNCTG 2 cut(s) 157, 569
PsuI RGATCY 2 cut(s) 142, 243
PvuI CGATCG 1 cut(s) 683
RsaI GTAC 2 cut(s) 115, 1139
RsaNI GTAC 2 cut(s) 114, 1138
RseI CAYNNNNRTG 1 cut(s) 1335
SapI GCTCTTC 1 cut(s) 1416
SaqAI TTAA 3 cut(s) 575, 1487, 1582
SatI GCNGC 6 cut(s) 259, 378, 1095, 1185, 1407, 1524
Sau96I GGNCC 6 cut(s) 92, 191, 403, 689, 1236, 1362
SchI GAGTC 2 cut(s) 209, 751
ScrFI CCNGG 2 cut(s) 206, 222
SduI GDGCHC 1 cut(s) 1512
SfaNI GCATC 3 cut(s) 174, 295, 1153
Sfr274I CTCGAG 1 cut(s) 1127
SinI GGWCC 4 cut(s) 92, 403, 689, 1362
SlaI CTCGAG 1 cut(s) 1127
SmiMI CAYNNNNRTG 1 cut(s) 1335
SmlI CTYRAG 3 cut(s) 917, 992, 1127
SmoI CTYRAG 3 cut(s) 917, 992, 1127
Sse9I AATT 4 cut(s) 315, 576, 1010, 1489
SseBI AGGCCT 1 cut(s) 287
SspMI CTAG 3 cut(s) 660, 1277, 1319
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 204, 220
StyI CCWWGG 4 cut(s) 282, 288, 659, 1470
TaaI ACNGT 1 cut(s) 653
TaiI ACGT 4 cut(s) 478, 795, 1270, 1396
TaqII GACCGA 1 cut(s) 762
TasI AATT 4 cut(s) 315, 576, 1010, 1489
TatI WGTACW 1 cut(s) 1137
TauI GCSGC 4 cut(s) 380, 1097, 1187, 1526
Tru1I TTAA 3 cut(s) 575, 1487, 1582
Tru9I TTAA 3 cut(s) 575, 1487, 1582
TseFI GTSAC 2 cut(s) 706, 754
TseI GCWGC 2 cut(s) 258, 1406
Tsp45I GTSAC 2 cut(s) 706, 754
TspDTI ATGAA 8 cut(s) 17, 429, 869, 882, 1065, 1237, 1314, 1410
TspGWI ACGGA 1 cut(s) 637
VpaK11BI GGWCC 4 cut(s) 92, 403, 689, 1362
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XhoI CTCGAG 1 cut(s) 1127
XmaJI CCTAGG 1 cut(s) 659
XspI CTAG 3 cut(s) 660, 1277, 1319
Zsp2I ATGCAT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.