AT3G32180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Reverse (-)
13162413 .. 13162787
375 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G32180.1

Sequence Viewer

Length: 303 bp
ATGGAGAACGAAGGAGCCTCTACCGTCTGCCCTCTCGACCCTGGCAGTCCTCTGTTCCTCTCCGGCGATGGCTTCGAGCCTGTCGGCGTTCTCTTTCCCCACGCCGTCTTCCACTTCCCTTCGAAGGAGAAAAAAGAAGCGTACGACTTTATGGATAAGGTCGTCGACAAGCATCGCCGCCTATGTGATGAGAGGCAGCGGTTGGGCCTCAAGAATGAATCCGAGGTTGACGTCAAGAACGAGGGGTCCACCTCGAATCCTGAACCAGCAGTGAAGGAGCCTGTTCCCGAGCCTCTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

11.09

Weight (kDa)

4.88

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3287 PF11690 3 - 73 6.1e-25 Protein of unknown function (DUF3287)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 165
AciI CCGC 2 cut(s) 178, 199
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 124
AjnI CCWGG 1 cut(s) 40
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 196
AspS9I GGNCC 2 cut(s) 205, 246
AsuII TTCGAA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 208
BccI CCATC 1 cut(s) 62
BceAI ACGGC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 42
BisI GCNGC 2 cut(s) 178, 197
BlsI GCNGC 2 cut(s) 179, 198
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 205, 246
BmiI GGNNCC 3 cut(s) 16, 247, 279
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 100
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 40, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 2 cut(s) 40, 222
BseLI CCNNNNNNNGG 1 cut(s) 124
BseXI GCAGC 1 cut(s) 208
BshFI GGCC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 1 cut(s) 63
BsiWI CGTACG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 124
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 122
BspACI CCGC 2 cut(s) 178, 199
BspANI GGCC 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 16, 247, 279
BspT104I TTCGAA 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 40, 222
BssNI GRCGYC 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 300
BstACI GRCGYC 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 122
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 208
BstV2I GAAGAC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 207
BtgZI GCGATG 2 cut(s) 81, 158
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 205, 246
Csp6I GTAC 1 cut(s) 142
CviJI RGCY 6 cut(s) 17, 72, 79, 207, 280, 292
CviKI_1 RGCY 6 cut(s) 17, 72, 79, 207, 280, 292
CviQI GTAC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 40
FaiI YATR 2 cut(s) 152, 184
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 178, 197
Fsp4HI GCNGC 2 cut(s) 178, 197
GluI GCNGC 2 cut(s) 178, 197
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 231
HincII GTYRAC 2 cut(s) 166, 229
HindII GTYRAC 2 cut(s) 166, 229
HinfI GANTC 2 cut(s) 218, 256
HpaII CCGG 1 cut(s) 63
Hpy166II GTNNAC 3 cut(s) 166, 229, 249
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 5 cut(s) 35, 211, 235, 260, 287
Hpy8I GTNNAC 3 cut(s) 166, 229, 249
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 4 cut(s) 5, 118, 129, 268
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 14, 277
LpnPI CCDG 7 cut(s) 27, 54, 76, 93, 273, 279, 294
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 1 cut(s) 181
MaeII ACGT 1 cut(s) 231
MboII GAAGA 2 cut(s) 100, 287
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 42
NlaIV GGNNCC 3 cut(s) 16, 247, 279
NspV TTCGAA 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 2 cut(s) 218, 256
Pfl23II CGTACG 1 cut(s) 141
PkrI GCNGC 2 cut(s) 179, 198
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 3 cut(s) 16, 247, 279
PspPI GGNCC 2 cut(s) 205, 246
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SalI GTCGAC 1 cut(s) 164
SatI GCNGC 2 cut(s) 178, 197
Sau96I GGNCC 2 cut(s) 205, 246
ScrFI CCNGG 1 cut(s) 42
SetI ASST 4 cut(s) 162, 228, 234, 254
SfaNI GCATC 1 cut(s) 181
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SsiI CCGC 2 cut(s) 178, 199
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 234
TaqI TCGA 5 cut(s) 36, 75, 122, 165, 254
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 2 cut(s) 218, 256
TscAI CASTG 1 cut(s) 276
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 231
TspRI CASTG 1 cut(s) 276
VpaK11BI GGWCC 1 cut(s) 246
XmiI GTMKAC 1 cut(s) 165
ZraI GACGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.