AT5G35604

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004040
Physical Location & Seq
Reverse (-)
13788205 .. 13789345
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35604.1

Sequence Viewer

Length: 897 bp
ATGTCAACCGGGGCCATTGCTTTTCGGCGCAAGGTGCTGTCCAAGCCAATGACTGAGGCTTGCGAGGAGAACAACGTGTTCCTCGCGACTGCCATTGACAAGGAGGATTACACCTGGATCCTTCTTGTCGATGATGGTAGCGCCGAAGGAGCAAGATCTGCTTATGCTTTCAGGCCAACCCTCCACCGTGAGCACCGCGGAGGTCGTTCTCCTCGAAGGCCATCTCGTGGTAACTCTTCTCCGCGGAGGGACAAAGCAAGGGCTAGGACCGACTGTTCTCCCAGATTGTCGCCTCCTTCTCGAACAATGGGTCCTCCTCCTCCTGTCGCCACGTCCCCCTCTTCACAATGGAGTGGAGAGAAAACGGACCACAATACGGTTCCTCAAAAGGAGGGTGGTGAGCATCGAGATACTCCCCCGAGGTCAAAGGTCGTGCTTGTTCCTCAAACCAAATCCCTTATAGCAATGGAAAAAGAGGGCAATGTCTTTCGCTGTTTCAAAACCCGCAAAGATGCGATTTTGCCAACTTTTGACAAATGGCGCCCGACAATTCGCGAACGCTACTTGCTCCATGCCCACCATACAGTCCGGGCAAACAGCAAATTCAATAACATGGTTGAGCACTACGAGGGGCTGCTACTCAGCTGGGAACAAGAGATCAACTCGTGGAGGAACAAATTCTCCTCTCTCGAGGCTGATCACCGCTCCTTTTCGGACTCTATGAATGACAAGCACGAGCTGGAAGAACAAGTTGACCACTTCAAATCCAAACTCTTGAAGTCAAATGACGAACTGCAAGCGCAGTATGGTCGATATGATGACCTTCAAACTGAACTTGCGGGTGTCCGAGATAGACTTTTCCAATCTGAGTCGGCTGCCGACGCCATGAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

34.05

Weight (kDa)

9.05

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 705
AccII CGCG 4 cut(s) 86, 198, 244, 555
AciI CCGC 7 cut(s) 196, 198, 242, 244, 505, 703, 839
AclWI GGATC 2 cut(s) 112, 125
AcsI RAATTY 2 cut(s) 602, 677
AcyI GRCGYC 2 cut(s) 541, 882
AfiI CCNNNNNNNGG 2 cut(s) 227, 376
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 5 cut(s) 499, 607, 763, 778, 827
AjiI CACGTC 1 cut(s) 333
AjnI CCWGG 1 cut(s) 113
AloI GAACNNNNNNTCC 6 cut(s) 259, 291, 295, 327, 665, 697
AluBI AGCT 2 cut(s) 645, 739
AluI AGCT 2 cut(s) 645, 739
Alw21I GWGCWC 2 cut(s) 195, 624
AlwI GGATC 2 cut(s) 112, 125
Ama87I CYCGRG 2 cut(s) 418, 689
AoxI GGCC 3 cut(s) 12, 173, 218
ApeKI GCWGC 2 cut(s) 634, 875
ApoI RAATTY 2 cut(s) 602, 677
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
Asp700I GAANNNNTTC 1 cut(s) 677
AspLEI GCGC 4 cut(s) 30, 143, 543, 802
AspS9I GGNCC 4 cut(s) 12, 267, 311, 367
AsuC2I CCSGG 2 cut(s) 10, 590
AsuHPI GGTGA 2 cut(s) 410, 692
AvaI CYCGRG 2 cut(s) 418, 689
AvaII GGWCC 3 cut(s) 267, 311, 367
BamHI GGATCC 1 cut(s) 117
BanI GGYRCC 1 cut(s) 540
BauI CACGAG 3 cut(s) 225, 664, 734
Bbv12I GWGCWC 2 cut(s) 195, 624
BbvI GCAGC 2 cut(s) 621, 862
BccI CCATC 2 cut(s) 128, 229
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 1 cut(s) 115
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 2 cut(s) 10, 590
BfaI CTAG 1 cut(s) 264
BfoI RGCGCY 2 cut(s) 144, 544
BglII AGATCT 1 cut(s) 155
BisI GCNGC 2 cut(s) 635, 876
BlsI GCNGC 2 cut(s) 636, 877
Bme1390I CCNGG 3 cut(s) 10, 115, 590
Bme18I GGWCC 3 cut(s) 267, 311, 367
BmeT110I CYCGRG 2 cut(s) 418, 689
BmgBI CACGTC 1 cut(s) 333
BmgT120I GGNCC 4 cut(s) 12, 267, 311, 367
BmiI GGNNCC 5 cut(s) 13, 119, 312, 381, 542
BmrFI CCNGG 3 cut(s) 10, 115, 590
BmsI GCATC 2 cut(s) 412, 502
BplI GAGNNNNNCTC 2 cut(s) 647, 679
BpuMI CCSGG 2 cut(s) 10, 590
BsaHI GRCGYC 2 cut(s) 541, 882
BsaJI CCNNGG 4 cut(s) 9, 196, 242, 419
BsaXI ACNNNNNCTCC 2 cut(s) 665, 695
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 376
Bse3DI GCAATG 3 cut(s) 15, 471, 487
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 4 cut(s) 9, 196, 242, 419
BseLI CCNNNNNNNGG 2 cut(s) 227, 376
BseMI GCAATG 3 cut(s) 15, 471, 487
BseMII CTCAG 3 cut(s) 45, 655, 858
BseRI GAGGAG 5 cut(s) 80, 201, 306, 309, 673
BseXI GCAGC 2 cut(s) 621, 862
BseYI CCCAGC 1 cut(s) 645
Bsh1236I CGCG 4 cut(s) 86, 198, 244, 555
BshFI GGCC 3 cut(s) 14, 175, 220
BshNI GGYRCC 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 195, 624
BsiHKCI CYCGRG 2 cut(s) 418, 689
BsiSI CCGG 2 cut(s) 9, 589
BslFI GGGAC 2 cut(s) 263, 319
BslI CCNNNNNNNGG 2 cut(s) 227, 376
BsmFI GGGAC 2 cut(s) 263, 319
BsnI GGCC 3 cut(s) 14, 175, 220
BsoBI CYCGRG 2 cut(s) 418, 689
Bsp1286I GDGCHC 2 cut(s) 195, 624
Bsp143I GATC 4 cut(s) 117, 155, 657, 697
Bsp68I TCGCGA 2 cut(s) 86, 555
BspACI CCGC 7 cut(s) 196, 198, 242, 244, 505, 703, 839
BspANI GGCC 3 cut(s) 14, 175, 220
BspCNI CTCAG 3 cut(s) 46, 654, 859
BspFNI CGCG 4 cut(s) 86, 198, 244, 555
BspLI GGNNCC 5 cut(s) 13, 119, 312, 381, 542
BspPI GGATC 2 cut(s) 112, 125
BspT107I GGYRCC 1 cut(s) 540
BsrBI CCGCTC 1 cut(s) 705
BsrDI GCAATG 3 cut(s) 15, 471, 487
BssECI CCNNGG 4 cut(s) 9, 196, 242, 419
BssMI GATC 4 cut(s) 117, 155, 657, 697
BssNI GRCGYC 2 cut(s) 541, 882
BssSI CACGAG 3 cut(s) 225, 664, 734
Bst2BI CACGAG 3 cut(s) 225, 664, 734
Bst2UI CCWGG 1 cut(s) 115
Bst4CI ACNGT 4 cut(s) 188, 275, 379, 586
Bst6I CTCTTC 2 cut(s) 241, 346
BstACI GRCGYC 2 cut(s) 541, 882
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 61, 798
BstDEI CTNAG 3 cut(s) 54, 641, 867
BstDSI CCRYGG 2 cut(s) 196, 242
BstFNI CGCG 4 cut(s) 86, 198, 244, 555
BstH2I RGCGCY 2 cut(s) 144, 544
BstHHI GCGC 4 cut(s) 30, 143, 543, 802
BstKTI GATC 4 cut(s) 120, 158, 660, 700
BstMBI GATC 4 cut(s) 117, 155, 657, 697
BstMWI GCNNNNNNNGC 5 cut(s) 34, 43, 149, 158, 881
BstNI CCWGG 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 8, 113, 588
BstUI CGCG 4 cut(s) 86, 198, 244, 555
BstV1I GCAGC 2 cut(s) 621, 862
BstX2I RGATCY 2 cut(s) 117, 155
BstYI RGATCY 2 cut(s) 117, 155
BsuRI GGCC 3 cut(s) 14, 175, 220
BtgI CCRYGG 2 cut(s) 196, 242
BtrI CACGTC 1 cut(s) 333
BtuMI TCGCGA 2 cut(s) 86, 555
Cac8I GCNNGC 2 cut(s) 61, 798
CfoI GCGC 4 cut(s) 30, 143, 543, 802
Cfr13I GGNCC 4 cut(s) 12, 267, 311, 367
Cfr42I CCGCGG 2 cut(s) 199, 245
CseI GACGC 1 cut(s) 890
CviAII CATG 3 cut(s) 572, 613, 886
DdeI CTNAG 3 cut(s) 54, 641, 867
DinI GGCGCC 1 cut(s) 542
DpnI GATC 4 cut(s) 119, 157, 659, 699
DpnII GATC 4 cut(s) 117, 155, 657, 697
Eam1104I CTCTTC 2 cut(s) 241, 346
EarI CTCTTC 2 cut(s) 241, 346
Eco47I GGWCC 3 cut(s) 267, 311, 367
Eco88I CYCGRG 2 cut(s) 418, 689
EcoO109I RGGNCCY 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 113
EgeI GGCGCC 1 cut(s) 542
EheI GGCGCC 1 cut(s) 542
FaeI CATG 3 cut(s) 575, 616, 889
FaiI YATR 9 cut(s) 165, 461, 573, 582, 614, 722, 807, 816, 887
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 2 cut(s) 263, 319
FatI CATG 3 cut(s) 571, 612, 885
FauI CCCGC 2 cut(s) 512, 832
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 2 cut(s) 635, 876
Fsp4HI GCNGC 2 cut(s) 635, 876
FspBI CTAG 1 cut(s) 264
GlaI GCGC 4 cut(s) 29, 142, 542, 801
GluI GCNGC 2 cut(s) 635, 876
GsaI CCCAGC 1 cut(s) 649
HaeII RGCGCY 2 cut(s) 144, 544
HaeIII GGCC 3 cut(s) 14, 175, 220
HapII CCGG 2 cut(s) 9, 589
HgaI GACGC 1 cut(s) 890
HhaI GCGC 4 cut(s) 30, 143, 543, 802
Hin1I GRCGYC 2 cut(s) 541, 882
Hin1II CATG 3 cut(s) 575, 616, 889
Hin6I GCGC 4 cut(s) 28, 141, 541, 800
HinP1I GCGC 4 cut(s) 28, 141, 541, 800
HincII GTYRAC 2 cut(s) 6, 754
HindII GTYRAC 2 cut(s) 6, 754
HinfI GANTC 2 cut(s) 716, 869
HpaII CCGG 2 cut(s) 9, 589
HphI GGTGA 2 cut(s) 410, 692
Hpy166II GTNNAC 2 cut(s) 6, 754
Hpy188I TCNGA 3 cut(s) 715, 848, 868
Hpy188III TCNNGA 6 cut(s) 85, 300, 407, 554, 689, 775
Hpy8I GTNNAC 2 cut(s) 6, 754
Hpy99I CGWCG 1 cut(s) 884
HpyAV CCTTC 5 cut(s) 131, 140, 210, 306, 833
HpyCH4III ACNGT 4 cut(s) 188, 275, 379, 586
HpyCH4IV ACGT 2 cut(s) 75, 332
HpyCH4V TGCA 1 cut(s) 796
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 43, 149, 158, 881
HpyF3I CTNAG 3 cut(s) 54, 641, 867
HpySE526I ACGT 2 cut(s) 75, 332
Hsp92I GRCGYC 2 cut(s) 541, 882
Hsp92II CATG 3 cut(s) 575, 616, 889
HspAI GCGC 4 cut(s) 28, 141, 541, 800
KasI GGCGCC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 697
KspI CCGCGG 2 cut(s) 199, 245
Kzo9I GATC 4 cut(s) 117, 155, 657, 697
LmnI GCTCC 3 cut(s) 149, 573, 710
LpnPI CCDG 9 cut(s) 22, 100, 127, 157, 295, 336, 602, 631, 725
Lsp1109I GCAGC 2 cut(s) 621, 862
LweI GCATC 2 cut(s) 412, 502
MaeI CTAG 1 cut(s) 264
MaeII ACGT 2 cut(s) 75, 332
MaeIII GTNAC 1 cut(s) 230
MalI GATC 4 cut(s) 119, 157, 659, 699
MbiI CCGCTC 1 cut(s) 705
MboI GATC 4 cut(s) 117, 155, 657, 697
MboII GAAGA 3 cut(s) 228, 333, 755
MflI RGATCY 2 cut(s) 117, 155
MhlI GDGCHC 2 cut(s) 195, 624
MluCI AATT 3 cut(s) 549, 602, 677
Mly113I GGCGCC 1 cut(s) 541
MlyI GAGTC 2 cut(s) 710, 878
MroXI GAANNNNTTC 1 cut(s) 677
MspA1I CMGCKG 3 cut(s) 198, 244, 645
MspI CCGG 2 cut(s) 9, 589
MspR9I CCNGG 3 cut(s) 10, 115, 590
MvaI CCWGG 1 cut(s) 115
MvnI CGCG 4 cut(s) 86, 198, 244, 555
MwoI GCNNNNNNNGC 5 cut(s) 34, 43, 149, 158, 881
NarI GGCGCC 1 cut(s) 541
NciI CCSGG 2 cut(s) 10, 590
NdeII GATC 4 cut(s) 117, 155, 657, 697
NlaIII CATG 3 cut(s) 575, 616, 889
NlaIV GGNNCC 5 cut(s) 13, 119, 312, 381, 542
NruI TCGCGA 2 cut(s) 86, 555
PaeR7I CTCGAG 1 cut(s) 689
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 677
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 2 cut(s) 636, 877
PleI GAGTC 2 cut(s) 710, 877
PluTI GGCGCC 1 cut(s) 544
PpsI GAGTC 2 cut(s) 710, 877
PpuMI RGGWCCY 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 645
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 5 cut(s) 13, 119, 312, 381, 542
PspPI GGNCC 4 cut(s) 12, 267, 311, 367
PspPPI RGGWCCY 1 cut(s) 311
PsuI RGATCY 2 cut(s) 117, 155
PvuII CAGCTG 1 cut(s) 645
RruI TCGCGA 2 cut(s) 86, 555
SacII CCGCGG 2 cut(s) 199, 245
SatI GCNGC 2 cut(s) 635, 876
Sau3AI GATC 4 cut(s) 117, 155, 657, 697
Sau96I GGNCC 4 cut(s) 12, 267, 311, 367
SchI GAGTC 2 cut(s) 710, 878
ScrFI CCNGG 3 cut(s) 10, 115, 590
SduI GDGCHC 2 cut(s) 195, 624
SfaNI GCATC 2 cut(s) 412, 502
SfoI GGCGCC 1 cut(s) 542
Sfr274I CTCGAG 1 cut(s) 689
Sfr303I CCGCGG 2 cut(s) 199, 245
SgrBI CCGCGG 2 cut(s) 199, 245
SinI GGWCC 3 cut(s) 267, 311, 367
SlaI CTCGAG 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 3 cut(s) 549, 602, 677
SsiI CCGC 7 cut(s) 196, 198, 242, 244, 505, 703, 839
SspDI GGCGCC 1 cut(s) 540
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 8, 113, 588
TaaI ACNGT 4 cut(s) 188, 275, 379, 586
TaiI ACGT 2 cut(s) 78, 335
TaqI TCGA 6 cut(s) 129, 214, 301, 406, 690, 811
TaqII GACCGA 1 cut(s) 284
TasI AATT 3 cut(s) 549, 602, 677
TseI GCWGC 2 cut(s) 634, 875
TspDTI ATGAA 1 cut(s) 737
TspGWI ACGGA 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 3 cut(s) 267, 311, 367
XapI RAATTY 2 cut(s) 602, 677
XhoI CTCGAG 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 677
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.