AT3G32190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Reverse (-)
13163278 .. 13164557
1280 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G32190.1

Sequence Viewer

Length: 1077 bp
ATGCCTAGTCTTCGAACGAGTCATAGGGCCGGTTGGTTCCAACAAAGCTTCCTACGTTACAAGCTGCTCCTCGGCTACAAGCTGCTCCATTGGTTCAAGTTCCAAGTCTTGTCCGACAATAGTCATATCAACGCCCATCACTCATCTCGGACAAACAGCGAACTCAATGACATGATTCAGTATTACGAGAGGTTGTTGCTTGACAGGGAACAGGACGTTATGGCCTGGAAGGATAAGTCTTCTTCTCTTGAGTCCGATCTCCGCTCTTCCACTGAAGTCAAGCAAAAATTGGAAGATCAATTGGAAAATCTTTCCTCCAAACTCATGCAGTCAAACGGCGAGTTGCAGGATCAGTATCAGAGGTATGACAAGATTCAGGAGGAACTCTCGAATGCTCGGGGCAGACTCTCAGAATCTGAATCCAATGCTTACGACTTGTCCAACCAGCTATCCAAATTGCAGGCCAAATCAGCATCAAAAGCCAGGAAAGAAGTAAAAGGTCACGGGATAGAATGGATTCAAGGGGCGATCGTTTTCATCCAAACCGAGAAAACTAGGACAGAGCTGGAATCAGACATCAAGGAATATGAAAGCAACCTCCTCCTTCTGGACCAAACCCATGACGAGGATTTTTCCGAGGAGCAAGAAAGGTCCGAATTGAAAGCCGTTTTAGCAGAGAAGCGAAGTCGCTTGGCCGCTCTTCCCTCTTCGTCATTCAACCCTCAACAGTTCGAGGAGTTCTTTACCGAGTCGCCTCCTTTGAGCGAATCCGGTTTAGATTGGACTAGTCCCAGTGATCCCGTGGAACCCGAAGTACCTATCATGTCGGTGGTAACTCCTGAACCGGTAGCCACTCCGCCAGAAATTCTTCCATCCACGAATACCGTAACAGTGGTCATCGAGGATGACGATGGTTCGGAATCTGGGATTCCTACTGAACAGCCCGTCACTGACGTAACGGATGAGACTGAGACCCAGTCGTGGACCCAGTCGATCTTGAGCCAACTCAGACTGAATTCGAGGTGGCTGAGGATCCGTAGTGGCTGCGTCCACTTAATGGGGGTTGTCCCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

358

Amino Acids

40.91

Weight (kDa)

4.82

Isoelectric Point (pI)

64.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1057
AccBSI CCGCTC 2 cut(s) 264, 698
AciI CCGC 3 cut(s) 262, 696, 857
AclWI GGATC 4 cut(s) 357, 791, 1027, 1040
AcoI YGGCCR 1 cut(s) 693
AcsI RAATTY 2 cut(s) 864, 1015
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 816
AfiI CCNNNNNNNGG 4 cut(s) 607, 625, 981, 1057
AgeI ACCGGT 1 cut(s) 844
AgsI TTSAA 4 cut(s) 97, 521, 661, 718
AhlI ACTAGT 1 cut(s) 785
AjnI CCWGG 2 cut(s) 224, 482
AluBI AGCT 5 cut(s) 48, 64, 82, 448, 565
AluI AGCT 5 cut(s) 48, 64, 82, 448, 565
Alw26I GTCTC 2 cut(s) 959, 965
AlwI GGATC 4 cut(s) 357, 791, 1027, 1040
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 396
AoxI GGCC 4 cut(s) 27, 222, 462, 693
ApeKI GCWGC 3 cut(s) 64, 82, 1044
ApoI RAATTY 2 cut(s) 864, 1015
AsiGI ACCGGT 1 cut(s) 844
Asp700I GAANNNNTTC 2 cut(s) 516, 867
AspS9I GGNCC 4 cut(s) 27, 610, 651, 984
AsuII TTCGAA 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 396
AvaII GGWCC 3 cut(s) 610, 651, 984
BamHI GGATCC 1 cut(s) 1032
BbsI GAAGAC 2 cut(s) 2, 231
BbvCI CCTCAGC 1 cut(s) 1028
BbvI GCAGC 3 cut(s) 51, 69, 1031
BccI CCATC 3 cut(s) 144, 880, 905
BceAI ACGGC 2 cut(s) 352, 650
BciT130I CCWGG 2 cut(s) 226, 484
BcoDI GTCTC 2 cut(s) 959, 965
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 3 cut(s) 6, 555, 786
BisI GCNGC 4 cut(s) 65, 83, 696, 1045
BlsI GCNGC 4 cut(s) 66, 84, 697, 1046
Bme1390I CCNGG 2 cut(s) 226, 484
Bme18I GGWCC 3 cut(s) 610, 651, 984
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 4 cut(s) 27, 610, 651, 984
BmiI GGNNCC 4 cut(s) 38, 807, 986, 1034
BmrFI CCNGG 2 cut(s) 226, 484
BmrI ACTGGG 3 cut(s) 786, 970, 982
BmsI GCATC 1 cut(s) 482
BmuI ACTGGG 3 cut(s) 786, 970, 982
BoxI GACNNNNGTC 1 cut(s) 120
BpiI GAAGAC 2 cut(s) 2, 231
BplI GAGNNNNNCTC 2 cut(s) 371, 403
Bpu10I CCTNAGC 1 cut(s) 1028
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 2 cut(s) 269, 1018
BsaBI GATNNNNATC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 965
BsaJI CCNNGG 3 cut(s) 70, 636, 801
BsaWI WCCGGW 2 cut(s) 770, 844
Bsc4I CCNNNNNNNGG 4 cut(s) 607, 625, 981, 1057
Bse118I RCCGGY 2 cut(s) 29, 844
Bse1I ACTGG 3 cut(s) 792, 976, 988
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 2 cut(s) 226, 484
BseDI CCNNGG 3 cut(s) 70, 636, 801
BseGI GGATG 4 cut(s) 537, 872, 910, 967
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 4 cut(s) 607, 625, 981, 1057
BseMII CTCAG 4 cut(s) 423, 960, 1019, 1021
BseNI ACTGG 3 cut(s) 792, 976, 988
BseRI GAGGAG 4 cut(s) 59, 590, 653, 749
BseXI GCAGC 3 cut(s) 51, 69, 1031
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 4 cut(s) 29, 224, 464, 695
BshTI ACCGGT 1 cut(s) 844
BsiEI CGRYCG 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 396
BsiSI CCGG 3 cut(s) 30, 771, 845
BslFI GGGAC 2 cut(s) 774, 1052
BslI CCNNNNNNNGG 4 cut(s) 607, 625, 981, 1057
BsmAI GTCTC 2 cut(s) 959, 965
BsmFI GGGAC 2 cut(s) 774, 1052
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 4 cut(s) 29, 224, 464, 695
Bso31I GGTCTC 1 cut(s) 965
BsoBI CYCGRG 1 cut(s) 396
Bsp119I TTCGAA 1 cut(s) 13
Bsp143I GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
BspACI CCGC 3 cut(s) 262, 696, 857
BspANI GGCC 4 cut(s) 29, 224, 464, 695
BspCNI CTCAG 4 cut(s) 422, 961, 1020, 1020
BspLI GGNNCC 4 cut(s) 38, 807, 986, 1034
BspPI GGATC 4 cut(s) 357, 791, 1027, 1040
BspQI GCTCTTC 2 cut(s) 271, 705
BspT104I TTCGAA 1 cut(s) 13
BspTNI GGTCTC 1 cut(s) 965
BsrBI CCGCTC 2 cut(s) 264, 698
BsrFI RCCGGY 2 cut(s) 29, 844
BsrI ACTGG 3 cut(s) 792, 976, 988
BssAI RCCGGY 2 cut(s) 29, 844
BssECI CCNNGG 3 cut(s) 70, 636, 801
BssMI GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
Bst2UI CCWGG 2 cut(s) 226, 484
Bst4CI ACNGT 3 cut(s) 729, 886, 892
Bst6I CTCTTC 3 cut(s) 271, 705, 712
BstBI TTCGAA 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 4 cut(s) 409, 969, 1007, 1028
BstDSI CCRYGG 1 cut(s) 801
BstF5I GGATG 4 cut(s) 537, 872, 910, 967
BstKTI GATC 7 cut(s) 259, 298, 352, 531, 799, 996, 1035
BstMAI GTCTC 2 cut(s) 959, 965
BstMBI GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 3 cut(s) 470, 479, 671
BstNI CCWGG 2 cut(s) 226, 484
BstPAI GACNNNNGTC 1 cut(s) 120
BstSCI CCNGG 2 cut(s) 224, 482
BstV1I GCAGC 3 cut(s) 51, 69, 1031
BstV2I GAAGAC 2 cut(s) 2, 231
BstX2I RGATCY 1 cut(s) 1032
BstYI RGATCY 1 cut(s) 1032
BsuRI GGCC 4 cut(s) 29, 224, 464, 695
BtgI CCRYGG 1 cut(s) 801
BtsCI GGATG 4 cut(s) 537, 872, 910, 967
BtsIMutI CAGTG 4 cut(s) 270, 799, 897, 948
Cac8I GCNNGC 1 cut(s) 462
CaiI CAGNNNCTG 1 cut(s) 416
Cfr10I RCCGGY 2 cut(s) 29, 844
Cfr13I GGNCC 4 cut(s) 27, 610, 651, 984
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 1 cut(s) 815
CspAI ACCGGT 1 cut(s) 844
CviAII CATG 4 cut(s) 172, 325, 620, 823
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 4 cut(s) 409, 969, 1007, 1028
DpnI GATC 7 cut(s) 258, 297, 351, 530, 798, 995, 1034
DpnII GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
EaeI YGGCCR 1 cut(s) 693
Eam1104I CTCTTC 3 cut(s) 271, 705, 712
EarI CTCTTC 3 cut(s) 271, 705, 712
EciI GGCGGA 1 cut(s) 846
Eco31I GGTCTC 1 cut(s) 965
Eco47I GGWCC 3 cut(s) 610, 651, 984
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 396
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 224, 482
FaeI CATG 4 cut(s) 175, 328, 623, 826
FaqI GGGAC 2 cut(s) 774, 1052
FatI CATG 4 cut(s) 171, 324, 619, 822
Fnu4HI GCNGC 4 cut(s) 65, 83, 696, 1045
FokI GGATG 4 cut(s) 524, 859, 917, 974
Fsp4HI GCNGC 4 cut(s) 65, 83, 696, 1045
FspBI CTAG 3 cut(s) 6, 555, 786
GluI GCNGC 4 cut(s) 65, 83, 696, 1045
HaeIII GGCC 4 cut(s) 29, 224, 464, 695
HapII CCGG 3 cut(s) 30, 771, 845
HgaI GACGC 1 cut(s) 1036
Hin1II CATG 4 cut(s) 175, 328, 623, 826
HindIII AAGCTT 1 cut(s) 46
HpaII CCGG 3 cut(s) 30, 771, 845
Hpy166II GTNNAC 2 cut(s) 984, 1051
Hpy188III TCNNGA 6 cut(s) 248, 377, 388, 608, 839, 997
Hpy8I GTNNAC 2 cut(s) 984, 1051
HpyAV CCTTC 2 cut(s) 223, 614
HpyCH4III ACNGT 3 cut(s) 729, 886, 892
HpyCH4IV ACGT 3 cut(s) 55, 216, 954
HpyCH4V TGCA 3 cut(s) 328, 346, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 470, 479, 671
HpyF3I CTNAG 4 cut(s) 409, 969, 1007, 1028
HpySE526I ACGT 3 cut(s) 55, 216, 954
Hsp92II CATG 4 cut(s) 175, 328, 623, 826
Kzo9I GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
LguI GCTCTTC 2 cut(s) 271, 705
LmnI GCTCC 3 cut(s) 72, 90, 640
Lsp1109I GCAGC 3 cut(s) 51, 69, 1031
LweI GCATC 1 cut(s) 482
MaeI CTAG 3 cut(s) 6, 555, 786
MaeII ACGT 3 cut(s) 55, 216, 954
MaeIII GTNAC 6 cut(s) 56, 500, 832, 886, 946, 955
MalI GATC 7 cut(s) 258, 297, 351, 530, 798, 995, 1034
MbiI CCGCTC 2 cut(s) 264, 698
MboI GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
MboII GAAGA 7 cut(s) 231, 234, 258, 305, 692, 699, 860
MfeI CAATTG 1 cut(s) 299
MflI RGATCY 1 cut(s) 1032
MluCI AATT 6 cut(s) 287, 299, 455, 656, 864, 1015
MlyI GAGTC 4 cut(s) 28, 260, 399, 758
MmeI TCCRAC 3 cut(s) 64, 138, 465
MroXI GAANNNNTTC 2 cut(s) 516, 867
MseI TTAA 1 cut(s) 1055
MslI CAYNNNNRTG 1 cut(s) 827
MspI CCGG 3 cut(s) 30, 771, 845
MspR9I CCNGG 2 cut(s) 226, 484
MunI CAATTG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 2 cut(s) 226, 484
MwoI GCNNNNNNNGC 3 cut(s) 470, 479, 671
NdeII GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
NlaIII CATG 4 cut(s) 175, 328, 623, 826
NlaIV GGNNCC 4 cut(s) 38, 807, 986, 1034
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 2 cut(s) 500, 946
NspV TTCGAA 1 cut(s) 13
PciSI GCTCTTC 2 cut(s) 271, 705
PcsI WCGNNNNNNNCGW 1 cut(s) 906
PctI GAATGC 1 cut(s) 397
PdmI GAANNNNTTC 2 cut(s) 516, 867
PfeI GAWTC 9 cut(s) 175, 373, 413, 419, 517, 569, 767, 920, 928
PflFI GACNNNGTC 2 cut(s) 976, 988
PflMI CCANNNNNTGG 1 cut(s) 1057
PinAI ACCGGT 1 cut(s) 844
PkrI GCNGC 4 cut(s) 66, 84, 697, 1046
Ple19I CGATCG 1 cut(s) 531
PleI GAGTC 4 cut(s) 27, 259, 399, 757
PpsI GAGTC 4 cut(s) 27, 259, 399, 757
PshAI GACNNNNGTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 224, 482
PspGI CCWGG 2 cut(s) 224, 482
PspN4I GGNNCC 4 cut(s) 38, 807, 986, 1034
PspPI GGNCC 4 cut(s) 27, 610, 651, 984
PsrI GAACNNNNNNTAC 2 cut(s) 798, 830
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 1032
PsyI GACNNNGTC 2 cut(s) 976, 988
PvuI CGATCG 1 cut(s) 531
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 1 cut(s) 827
SapI GCTCTTC 2 cut(s) 271, 705
SaqAI TTAA 1 cut(s) 1055
SatI GCNGC 4 cut(s) 65, 83, 696, 1045
Sau3AI GATC 7 cut(s) 256, 295, 349, 528, 796, 993, 1032
Sau96I GGNCC 4 cut(s) 27, 610, 651, 984
SchI GAGTC 4 cut(s) 28, 260, 399, 758
ScrFI CCNGG 2 cut(s) 226, 484
SfaNI GCATC 1 cut(s) 482
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 3 cut(s) 610, 651, 984
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 2 cut(s) 248, 997
SmoI CTYRAG 2 cut(s) 248, 997
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 6 cut(s) 287, 299, 455, 656, 864, 1015
SsiI CCGC 3 cut(s) 262, 696, 857
SspMI CTAG 3 cut(s) 6, 555, 786
StyD4I CCNGG 2 cut(s) 224, 482
TaaI ACNGT 3 cut(s) 729, 886, 892
TaiI ACGT 3 cut(s) 58, 219, 957
TaqI TCGA 6 cut(s) 13, 389, 732, 900, 992, 1019
TasI AATT 6 cut(s) 287, 299, 455, 656, 864, 1015
TauI GCSGC 1 cut(s) 698
TfiI GAWTC 9 cut(s) 175, 373, 413, 419, 517, 569, 767, 920, 928
Tru1I TTAA 1 cut(s) 1055
Tru9I TTAA 1 cut(s) 1055
TscAI CASTG 4 cut(s) 277, 799, 897, 955
TseFI GTSAC 2 cut(s) 500, 946
TseI GCWGC 3 cut(s) 64, 82, 1044
Tsp45I GTSAC 2 cut(s) 500, 946
TspDTI ATGAA 2 cut(s) 526, 603
TspGWI ACGGA 2 cut(s) 974, 1025
TspRI CASTG 4 cut(s) 277, 799, 897, 955
Tth111I GACNNNGTC 2 cut(s) 976, 988
Van91I CCANNNNNTGG 1 cut(s) 1057
VpaK11BI GGWCC 3 cut(s) 610, 651, 984
XapI RAATTY 2 cut(s) 864, 1015
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XmnI GAANNNNTTC 2 cut(s) 516, 867
XspI CTAG 3 cut(s) 6, 555, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.