AT3G43150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Forward (+)
15143695 .. 15144432
738 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G43150.1

Sequence Viewer

Length: 585 bp
ATGATCGCAGAAATTTACTTGGACAACACCTATAAAATGGAACGCGAAAGAGCCTGCACCGTCTGCCCCCTCGACCCCGGGAGTCCCTTGTTTCTGTCCGGCGATGGCTTCGAGCCCACCGGTGTCCTCTTTCCAAACGCTTTTTTCCACTTCCCTTCGAAAGATAAAAAGGAGGCGTACGACTTTATGGACAAGGTCGTCGACAAGCATCGCCGCTTGTACGATGAAAGGCAGCGTTTGGGCCTCAAGAGGCAAACGCTGGAGGCCCGAATTGCCCGTATCGAGAGGAAGATAAGGGCTATGGAGAGCGACCCTTTTCAATGGGAGTGGAGGAACTTCGACTGCGTGGCGAAGATCCCGCACATGCTCCGGATGTACCTTCGTGCTCGAGGCCAAGTCTCCGGTCCTGTAAACGCTCCGGTTCAAGTGATCCCGTCTGACAGTGAGGACAGTGACGACGAGGAGGAGGACAGTAACCCCTGGAAGCGCGTGAAGAATGAACCCGACGTGGACGTCAAAAACGAGGCGTCCACTTCGAACCCCGAACCGGCGATGCAGGAGCCTGTTCCTGAGCCCCTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

22.48

Weight (kDa)

5.06

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3287 PF11690 17 - 133 3.9e-47 Protein of unknown function (DUF3287)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 197, 512
AatII GACGTC 1 cut(s) 516
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 45, 489
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 2 cut(s) 214, 359
AclWI GGATC 2 cut(s) 349, 424
AcsI RAATTY 1 cut(s) 12
AcyI GRCGYC 2 cut(s) 513, 527
AfaI GTAC 3 cut(s) 179, 221, 377
AfiI CCNNNNNNNGG 1 cut(s) 547
AgeI ACCGGT 1 cut(s) 119
AgsI TTSAA 2 cut(s) 320, 425
AjiI CACGTC 1 cut(s) 508
AjnI CCWGG 1 cut(s) 479
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 403
AlwI GGATC 2 cut(s) 349, 424
Ama87I CYCGRG 2 cut(s) 77, 387
Aor13HI TCCGGA 1 cut(s) 369
AoxI GGCC 3 cut(s) 241, 264, 391
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 1 cut(s) 12
AsiGI ACCGGT 1 cut(s) 119
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 3 cut(s) 241, 265, 404
AsuC2I CCSGG 2 cut(s) 78, 79
AsuII TTCGAA 2 cut(s) 158, 536
AvaI CYCGRG 2 cut(s) 77, 387
AvaII GGWCC 1 cut(s) 404
BanII GRGCYC 2 cut(s) 117, 576
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 98
BciT130I CCWGG 1 cut(s) 481
BcnI CCSGG 2 cut(s) 78, 79
BcoDI GTCTC 1 cut(s) 403
BisI GCNGC 2 cut(s) 214, 233
BlsI GCNGC 2 cut(s) 215, 234
Bme1390I CCNGG 3 cut(s) 78, 79, 481
Bme18I GGWCC 1 cut(s) 404
BmeT110I CYCGRG 2 cut(s) 77, 387
BmgBI CACGTC 1 cut(s) 508
BmgT120I GGNCC 3 cut(s) 241, 265, 404
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 3 cut(s) 78, 79, 481
BmsI GCATC 2 cut(s) 217, 543
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 2 cut(s) 158, 536
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 2 cut(s) 78, 79
BsaHI GRCGYC 2 cut(s) 513, 527
BsaJI CCNNGG 3 cut(s) 76, 77, 479
BsaWI WCCGGW 4 cut(s) 119, 369, 401, 418
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse118I RCCGGY 2 cut(s) 119, 547
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 3 cut(s) 76, 77, 479
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 561
BseRI GAGGAG 2 cut(s) 476, 479
BseXI GCAGC 1 cut(s) 244
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 45, 489
BshFI GGCC 3 cut(s) 243, 266, 393
BshTI ACCGGT 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 2 cut(s) 77, 387
BsiSI CCGG 7 cut(s) 78, 99, 120, 370, 402, 419, 548
BsiWI CGTACG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 1 cut(s) 403
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 3 cut(s) 243, 266, 393
BsoBI CYCGRG 2 cut(s) 77, 387
Bsp119I TTCGAA 2 cut(s) 158, 536
Bsp1286I GDGCHC 3 cut(s) 117, 388, 576
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 3 cut(s) 3, 354, 429
BspACI CCGC 2 cut(s) 214, 359
BspANI GGCC 3 cut(s) 243, 266, 393
BspCNI CTCAG 1 cut(s) 562
BspEI TCCGGA 1 cut(s) 369
BspFNI CGCG 2 cut(s) 45, 489
BspLI GGNNCC 1 cut(s) 561
BspPI GGATC 2 cut(s) 349, 424
BspT104I TTCGAA 2 cut(s) 158, 536
BsrFI RCCGGY 2 cut(s) 119, 547
BssAI RCCGGY 2 cut(s) 119, 547
BssECI CCNNGG 3 cut(s) 76, 77, 479
BssMI GATC 3 cut(s) 3, 354, 429
BssNI GRCGYC 2 cut(s) 513, 527
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 4 cut(s) 61, 443, 452, 473
BstACI GRCGYC 2 cut(s) 513, 527
BstAPI GCANNNNNTGC 1 cut(s) 63
BstBI TTCGAA 2 cut(s) 158, 536
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 1 cut(s) 570
BstF5I GGATG 1 cut(s) 378
BstFNI CGCG 2 cut(s) 45, 489
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 3 cut(s) 6, 357, 432
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 3, 354, 429
BstMWI GCNNNNNNNGC 2 cut(s) 63, 272
BstNI CCWGG 1 cut(s) 481
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 3 cut(s) 76, 77, 479
BstUI CGCG 2 cut(s) 45, 489
BstV1I GCAGC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuRI GGCC 3 cut(s) 243, 266, 393
BtgZI GCGATG 3 cut(s) 117, 194, 566
BtrI CACGTC 1 cut(s) 508
BtsCI GGATG 1 cut(s) 378
BtsIMutI CAGTG 2 cut(s) 448, 457
Cac8I GCNNGC 1 cut(s) 55
CfoI GCGC 1 cut(s) 489
Cfr10I RCCGGY 2 cut(s) 119, 547
Cfr13I GGNCC 3 cut(s) 241, 265, 404
Cfr9I CCCGGG 1 cut(s) 77
CseI GACGC 1 cut(s) 516
Csp6I GTAC 3 cut(s) 178, 220, 376
CspAI ACCGGT 1 cut(s) 119
CspCI CAANNNNNGTGG 2 cut(s) 308, 343
CviAII CATG 1 cut(s) 364
CviJI RGCY 9 cut(s) 53, 108, 115, 243, 266, 299, 393, 562, 574
CviKI_1 RGCY 9 cut(s) 53, 108, 115, 243, 266, 299, 393, 562, 574
CviQI GTAC 3 cut(s) 178, 220, 376
DdeI CTNAG 1 cut(s) 570
DpnI GATC 3 cut(s) 5, 356, 431
DpnII GATC 3 cut(s) 3, 354, 429
DrdI GACNNNNNNGTC 2 cut(s) 197, 512
DseDI GACNNNNNNGTC 2 cut(s) 197, 512
Eco24I GRGCYC 2 cut(s) 117, 576
Eco47I GGWCC 1 cut(s) 404
Eco88I CYCGRG 2 cut(s) 77, 387
EcoRII CCWGG 1 cut(s) 479
EcoT38I GRGCYC 2 cut(s) 117, 576
FaeI CATG 1 cut(s) 367
FaiI YATR 4 cut(s) 33, 188, 302, 365
FaqI GGGAC 1 cut(s) 69
FatI CATG 1 cut(s) 363
FauI CCCGC 1 cut(s) 366
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 214, 233
FokI GGATG 1 cut(s) 385
FriOI GRGCYC 2 cut(s) 117, 576
Fsp4HI GCNGC 2 cut(s) 214, 233
GlaI GCGC 1 cut(s) 488
GluI GCNGC 2 cut(s) 214, 233
GsuI CTGGAG 1 cut(s) 281
HaeIII GGCC 3 cut(s) 243, 266, 393
HapII CCGG 7 cut(s) 78, 99, 120, 370, 402, 419, 548
HgaI GACGC 1 cut(s) 516
HhaI GCGC 1 cut(s) 489
Hin1I GRCGYC 2 cut(s) 513, 527
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 82
HpaII CCGG 7 cut(s) 78, 99, 120, 370, 402, 419, 548
Hpy166II GTNNAC 4 cut(s) 202, 412, 511, 531
Hpy188I TCNGA 1 cut(s) 439
Hpy188III TCNNGA 4 cut(s) 247, 283, 370, 569
Hpy8I GTNNAC 4 cut(s) 202, 412, 511, 531
Hpy99I CGWCG 3 cut(s) 203, 461, 509
HpyAV CCTTC 2 cut(s) 165, 389
HpyCH4III ACNGT 4 cut(s) 61, 443, 452, 473
HpyCH4IV ACGT 2 cut(s) 507, 513
HpyCH4V TGCA 2 cut(s) 57, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 272
HpyF3I CTNAG 1 cut(s) 570
HpySE526I ACGT 2 cut(s) 507, 513
Hsp92I GRCGYC 2 cut(s) 513, 527
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 3 cut(s) 3, 354, 429
LmnI GCTCC 3 cut(s) 372, 421, 559
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 2 cut(s) 217, 543
MaeII ACGT 2 cut(s) 507, 513
MaeIII GTNAC 2 cut(s) 452, 473
MalI GATC 3 cut(s) 5, 356, 431
MboI GATC 3 cut(s) 3, 354, 429
MboII GAAGA 3 cut(s) 301, 364, 505
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 3 cut(s) 117, 388, 576
MluCI AATT 2 cut(s) 12, 270
MlyI GAGTC 1 cut(s) 91
MroI TCCGGA 1 cut(s) 369
MspI CCGG 7 cut(s) 78, 99, 120, 370, 402, 419, 548
MspR9I CCNGG 3 cut(s) 78, 79, 481
MvaI CCWGG 1 cut(s) 481
MvnI CGCG 2 cut(s) 45, 489
MwoI GCNNNNNNNGC 2 cut(s) 63, 272
NciI CCSGG 2 cut(s) 78, 79
NdeII GATC 3 cut(s) 3, 354, 429
NlaIII CATG 1 cut(s) 367
NlaIV GGNNCC 1 cut(s) 561
NmuCI GTSAC 1 cut(s) 452
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 2 cut(s) 158, 536
PaeR7I CTCGAG 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 519
Pfl23II CGTACG 1 cut(s) 177
PflFI GACNNNGTC 1 cut(s) 194
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 2 cut(s) 215, 234
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 479
PspGI CCWGG 1 cut(s) 479
PspLI CGTACG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 561
PspPI GGNCC 3 cut(s) 241, 265, 404
PspXI VCTCGAGB 1 cut(s) 387
PsuI RGATCY 1 cut(s) 354
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 3 cut(s) 179, 221, 377
RsaNI GTAC 3 cut(s) 178, 220, 376
SalI GTCGAC 1 cut(s) 200
SatI GCNGC 2 cut(s) 214, 233
Sau3AI GATC 3 cut(s) 3, 354, 429
Sau96I GGNCC 3 cut(s) 241, 265, 404
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 3 cut(s) 78, 79, 481
SduI GDGCHC 3 cut(s) 117, 388, 576
SetI ASST 5 cut(s) 32, 198, 381, 510, 516
SfaNI GCATC 2 cut(s) 217, 543
Sfr274I CTCGAG 1 cut(s) 387
SfuI TTCGAA 2 cut(s) 158, 536
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 387
SmaI CCCGGG 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 245, 387
SmoI CTYRAG 2 cut(s) 245, 387
Sse9I AATT 2 cut(s) 12, 270
SsiI CCGC 2 cut(s) 214, 359
StyD4I CCNGG 3 cut(s) 76, 77, 479
TaaI ACNGT 4 cut(s) 61, 443, 452, 473
TaiI ACGT 2 cut(s) 510, 516
TaqI TCGA 8 cut(s) 72, 111, 158, 201, 282, 339, 388, 536
TasI AATT 2 cut(s) 12, 270
TauI GCSGC 1 cut(s) 216
TscAI CASTG 2 cut(s) 448, 457
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 2 cut(s) 240, 513
TspMI CCCGGG 1 cut(s) 77
TspRI CASTG 2 cut(s) 448, 457
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 1 cut(s) 12
XceI RCATGY 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 387
XmaI CCCGGG 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 201
ZraI GACGTC 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.