AT3G32160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Forward (+)
13145440 .. 13145953
514 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G32160.1

Sequence Viewer

Length: 447 bp
ATGGAGAATGAAGGAGCCTCTACCGTTTGCCCTCTCGACCCCGGCAACCCGCTGTTCCTGTCCGGCGATGGCTTCGAGCCCGCCAGCGTTCTCTTTCCTCACGCCGTTTTTCACTTCCCCTTGAAGGACAAGAAAGAAGCGTACGACTTTATGGACAAGGTCGTTGACAAGTATCGCCGCCTATGCGATGAAAGACAGCGGTTGGGCCTCAAGAGGCAAATGCTCGAGGCCCGAATTTCCTGTGTGGAAAGGAAGGTGAGGTCCATGGAGAGTGACCCCTTCCAGTGGGATTGGAGGAACTTCGACTGTGCGGCAAAGATCCCGCATATGCTTCGGATGTATCTCCGTGCTCGAGGTCAAGTTCCTTGCCCCGTAAATGCTCCGGTCGAAGTGATCCCGTCTGACAGCGAAGAGGACAACGACGAGGAGGAGGATAGCAACCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.09

Weight (kDa)

4.92

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3287 PF11690 3 - 121 5.7e-47 Protein of unknown function (DUF3287)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 50, 81, 178, 199, 311, 323
AclWI GGATC 2 cut(s) 313, 388
AcsI RAATTY 1 cut(s) 234
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 2 cut(s) 124, 285
AgsI TTSAA 1 cut(s) 124
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 2 cut(s) 313, 388
Ama87I CYCGRG 2 cut(s) 224, 351
AoxI GGCC 2 cut(s) 205, 228
ApoI RAATTY 1 cut(s) 234
AspS9I GGNCC 3 cut(s) 205, 229, 261
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 2 cut(s) 224, 351
AvaII GGWCC 1 cut(s) 261
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 352
BccI CCATC 1 cut(s) 62
BceAI ACGGC 1 cut(s) 89
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BcnI CCSGG 1 cut(s) 42
BisI GCNGC 2 cut(s) 178, 312
BlsI GCNGC 2 cut(s) 179, 313
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 261
BmeT110I CYCGRG 2 cut(s) 224, 351
BmgT120I GGNCC 3 cut(s) 205, 229, 261
BmiI GGNNCC 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 42
BsaJI CCNNGG 2 cut(s) 40, 264
BsaWI WCCGGW 1 cut(s) 382
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 285
Bse1I ACTGG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 40, 264
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 124, 285
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 2 cut(s) 440, 443
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 2 cut(s) 207, 230
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 352
BsiHKCI CYCGRG 2 cut(s) 224, 351
BsiSI CCGG 3 cut(s) 42, 63, 383
BsiWI CGTACG 1 cut(s) 141
BslI CCNNNNNNNGG 2 cut(s) 124, 285
BsnI GGCC 2 cut(s) 207, 230
BsoBI CYCGRG 2 cut(s) 224, 351
Bsp1286I GDGCHC 2 cut(s) 81, 352
Bsp143I GATC 2 cut(s) 318, 393
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 6 cut(s) 50, 81, 178, 199, 311, 323
BspANI GGCC 2 cut(s) 207, 230
BspLI GGNNCC 1 cut(s) 16
BspPI GGATC 2 cut(s) 313, 388
BsrI ACTGG 1 cut(s) 283
BssECI CCNNGG 2 cut(s) 40, 264
BssMI GATC 2 cut(s) 318, 393
BssT1I CCWWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 25, 308
Bst6I CTCTTC 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 81, 85
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 342
BstKTI GATC 2 cut(s) 321, 396
BstMBI GATC 2 cut(s) 318, 393
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 2 cut(s) 207, 230
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 2 cut(s) 81, 201
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 2 cut(s) 81, 85
Cfr13I GGNCC 3 cut(s) 205, 229, 261
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 265
CviJI RGCY 5 cut(s) 17, 72, 79, 207, 230
CviKI_1 RGCY 5 cut(s) 17, 72, 79, 207, 230
CviQI GTAC 1 cut(s) 142
DpnI GATC 2 cut(s) 320, 395
DpnII GATC 2 cut(s) 318, 393
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 261
Eco88I CYCGRG 2 cut(s) 224, 351
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 1 cut(s) 268
FaiI YATR 5 cut(s) 152, 184, 266, 327, 329
FatI CATG 1 cut(s) 264
FauI CCCGC 3 cut(s) 57, 88, 330
FauNDI CATATG 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 178, 312
FokI GGATG 1 cut(s) 349
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 2 cut(s) 178, 312
GluI GCNGC 2 cut(s) 178, 312
HaeIII GGCC 2 cut(s) 207, 230
HapII CCGG 3 cut(s) 42, 63, 383
Hin1II CATG 1 cut(s) 268
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HpaII CCGG 3 cut(s) 42, 63, 383
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 2 cut(s) 336, 403
Hpy188III TCNNGA 2 cut(s) 35, 211
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 4 cut(s) 5, 118, 247, 289
HpyCH4III ACNGT 2 cut(s) 25, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
Hsp92II CATG 1 cut(s) 268
Kzo9I GATC 2 cut(s) 318, 393
LmnI GCTCC 2 cut(s) 14, 385
LpnPI CCDG 7 cut(s) 55, 71, 76, 97, 253, 296, 396
MaeIII GTNAC 1 cut(s) 272
MalI GATC 2 cut(s) 320, 395
MboI GATC 2 cut(s) 318, 393
MboII GAAGA 1 cut(s) 422
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 2 cut(s) 81, 352
MluCI AATT 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 52, 199
MspI CCGG 3 cut(s) 42, 63, 383
MspR9I CCNGG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 183
NciI CCSGG 1 cut(s) 42
NcoI CCATGG 1 cut(s) 264
NdeI CATATG 1 cut(s) 327
NdeII GATC 2 cut(s) 318, 393
NlaIII CATG 1 cut(s) 268
NlaIV GGNNCC 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 272
PaeR7I CTCGAG 2 cut(s) 224, 351
Pfl23II CGTACG 1 cut(s) 141
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 2 cut(s) 179, 313
PspLI CGTACG 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 16
PspPI GGNCC 3 cut(s) 205, 229, 261
PspXI VCTCGAGB 2 cut(s) 224, 351
PsuI RGATCY 1 cut(s) 318
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SatI GCNGC 2 cut(s) 178, 312
Sau3AI GATC 2 cut(s) 318, 393
Sau96I GGNCC 3 cut(s) 205, 229, 261
ScrFI CCNGG 1 cut(s) 42
SduI GDGCHC 2 cut(s) 81, 352
SetI ASST 4 cut(s) 162, 258, 263, 358
Sfr274I CTCGAG 2 cut(s) 224, 351
SinI GGWCC 1 cut(s) 261
SlaI CTCGAG 2 cut(s) 224, 351
SmlI CTYRAG 3 cut(s) 209, 224, 351
SmoI CTYRAG 3 cut(s) 209, 224, 351
Sse9I AATT 1 cut(s) 234
SsiI CCGC 6 cut(s) 50, 81, 178, 199, 311, 323
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 25, 308
TaqI TCGA 6 cut(s) 36, 75, 225, 303, 352, 387
TasI AATT 1 cut(s) 234
TauI GCSGC 2 cut(s) 180, 314
TscAI CASTG 1 cut(s) 290
TseFI GTSAC 1 cut(s) 272
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 24, 204
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 1 cut(s) 290
Tth111I GACNNNGTC 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 261
XapI RAATTY 1 cut(s) 234
XhoI CTCGAG 2 cut(s) 224, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.