AT3G32200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004040
Physical Location & Seq
Reverse (-)
13171081 .. 13171527
447 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G32200.1

Sequence Viewer

Length: 447 bp
ATGACAAAGGCTTGTGAGGCGAACAAGTCATTCCTTCAGTCTGCTGTCGACAAGAAAGAATACAGTAGAACTCTGCTGGTCGACGATGGTAGCGACGAAGGGGCGAGATCTGCCGGTGCCTTCAGACCTACACCTCACCGTAAAGGTCATGGCGGCCGGTCACCTCGTAGAGACCAATCACCACGTCGTGACTCACCTCAGACTGCTTCTCGGACTAGGTTAAGCAGCGAACTGATAGCAGACTTGCTTCAGAAAAAGAGAGAAAGACCAACTCGCAGCAGTTCCTTTCCTAAGAGAGATAAGACTATAGCCCGAACTGATCGTTCTCCTCGATCATCTCCCCTACCCCGATCAATGGGTCCCCCTCCTCCAGTCATCGCATCTCCTTCTTCTAGATCTTGGGGTGAAAGACCGGTCGGAGTGCAAATCCTCACAAGAAGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.43

Weight (kDa)

11.48

Isoelectric Point (pI)

99.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AccI GTMKAC 2 cut(s) 48, 81
AciI CCGC 1 cut(s) 153
AcoI YGGCCR 1 cut(s) 154
AcuI CTGAAG 3 cut(s) 20, 106, 233
AdeI CACNNNGTG 1 cut(s) 188
AfiI CCNNNNNNNGG 1 cut(s) 355
AgeI ACCGGT 1 cut(s) 412
AjiI CACGTC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 165
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 225, 276
AsiGI ACCGGT 1 cut(s) 412
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 5 cut(s) 128, 153, 171, 186, 416
AvaII GGWCC 1 cut(s) 359
BanI GGYRCC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 237, 288
BccI CCATC 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 2 cut(s) 216, 393
BfmI CTRYAG 1 cut(s) 306
BglII AGATCT 2 cut(s) 107, 395
BisI GCNGC 3 cut(s) 154, 226, 277
BlsI GCNGC 3 cut(s) 155, 227, 278
Bme18I GGWCC 1 cut(s) 359
BmgBI CACGTC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 3 cut(s) 118, 360, 361
BmsI GCATC 1 cut(s) 389
BpmI CTGGAG 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 165
BsaWI WCCGGW 1 cut(s) 412
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 3 cut(s) 113, 156, 412
Bse1I ACTGG 1 cut(s) 371
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 2 cut(s) 318, 357
BseX3I CGGCCG 1 cut(s) 154
BseXI GCAGC 2 cut(s) 237, 288
Bsh1285I CGRYCG 2 cut(s) 157, 417
BshFI GGCC 1 cut(s) 156
BshNI GGYRCC 1 cut(s) 116
BshTI ACCGGT 1 cut(s) 412
BsiEI CGRYCG 2 cut(s) 157, 417
BsiSI CCGG 3 cut(s) 114, 157, 413
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 345
BsnI GGCC 1 cut(s) 156
Bso31I GGTCTC 1 cut(s) 165
Bsp143I GATC 5 cut(s) 107, 319, 332, 350, 395
BspACI CCGC 1 cut(s) 153
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 118, 360, 361
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 165
BsrFI RCCGGY 3 cut(s) 113, 156, 412
BsrI ACTGG 1 cut(s) 371
BssAI RCCGGY 3 cut(s) 113, 156, 412
BssMI GATC 5 cut(s) 107, 319, 332, 350, 395
Bst4CI ACNGT 2 cut(s) 65, 140
Bst6I CTCTTC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 198, 291
BstEII GGTNACC 1 cut(s) 159
BstKTI GATC 5 cut(s) 110, 322, 335, 353, 398
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 5 cut(s) 107, 319, 332, 350, 395
BstMCI CGRYCG 2 cut(s) 157, 417
BstMWI GCNNNNNNNGC 2 cut(s) 17, 110
BstPI GGTNACC 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 237, 288
BstX2I RGATCY 2 cut(s) 107, 395
BstYI RGATCY 2 cut(s) 107, 395
BstZI CGGCCG 1 cut(s) 154
BsuRI GGCC 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 361
BtrI CACGTC 1 cut(s) 185
Cfr10I RCCGGY 3 cut(s) 113, 156, 412
Cfr13I GGNCC 1 cut(s) 359
CspAI ACCGGT 1 cut(s) 412
CviAII CATG 1 cut(s) 149
CviJI RGCY 3 cut(s) 11, 156, 311
CviKI_1 RGCY 3 cut(s) 11, 156, 311
DdeI CTNAG 2 cut(s) 198, 291
DpnI GATC 5 cut(s) 109, 321, 334, 352, 397
DpnII GATC 5 cut(s) 107, 319, 332, 350, 395
DraIII CACNNNGTG 1 cut(s) 188
EaeI YGGCCR 1 cut(s) 154
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
EclXI CGGCCG 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 165
Eco47I GGWCC 1 cut(s) 359
Eco52I CGGCCG 1 cut(s) 154
Eco57I CTGAAG 3 cut(s) 20, 106, 233
Eco91I GGTNACC 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 159
FaeI CATG 1 cut(s) 152
FaiI YATR 2 cut(s) 150, 308
FaqI GGGAC 1 cut(s) 345
FatI CATG 1 cut(s) 148
FblI GTMKAC 2 cut(s) 48, 81
Fnu4HI GCNGC 3 cut(s) 154, 226, 277
Fsp4HI GCNGC 3 cut(s) 154, 226, 277
FspBI CTAG 2 cut(s) 216, 393
GluI GCNGC 3 cut(s) 154, 226, 277
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 1 cut(s) 156
HapII CCGG 3 cut(s) 114, 157, 413
Hin1II CATG 1 cut(s) 152
HincII GTYRAC 2 cut(s) 49, 82
HindII GTYRAC 2 cut(s) 49, 82
HinfI GANTC 1 cut(s) 191
HpaII CCGG 3 cut(s) 114, 157, 413
HphI GGTGA 5 cut(s) 128, 153, 171, 186, 416
Hpy166II GTNNAC 2 cut(s) 49, 82
Hpy188I TCNGA 5 cut(s) 125, 201, 213, 252, 419
Hpy188III TCNNGA 2 cut(s) 188, 393
Hpy8I GTNNAC 2 cut(s) 49, 82
Hpy99I CGWCG 3 cut(s) 86, 98, 189
HpyAV CCTTC 4 cut(s) 44, 92, 130, 396
HpyCH4III ACNGT 2 cut(s) 65, 140
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 1 cut(s) 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 110
HpyF3I CTNAG 2 cut(s) 198, 291
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 1 cut(s) 152
KflI GGGWCCC 1 cut(s) 359
Kzo9I GATC 5 cut(s) 107, 319, 332, 350, 395
LpnPI CCDG 5 cut(s) 62, 127, 170, 384, 426
Lsp1109I GCAGC 2 cut(s) 237, 288
LweI GCATC 1 cut(s) 389
MaeI CTAG 2 cut(s) 216, 393
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 2 cut(s) 159, 188
MalI GATC 5 cut(s) 109, 321, 334, 352, 397
MboI GATC 5 cut(s) 107, 319, 332, 350, 395
MboII GAAGA 1 cut(s) 381
MflI RGATCY 2 cut(s) 107, 395
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 8 cut(s) 10, 144, 174, 207, 339, 375, 378, 440
MseI TTAA 1 cut(s) 221
MspI CCGG 3 cut(s) 114, 157, 413
MwoI GCNNNNNNNGC 2 cut(s) 17, 110
NdeII GATC 5 cut(s) 107, 319, 332, 350, 395
NlaIII CATG 1 cut(s) 152
NlaIV GGNNCC 3 cut(s) 118, 360, 361
NmuCI GTSAC 2 cut(s) 159, 188
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 328
PinAI ACCGGT 1 cut(s) 412
PkrI GCNGC 3 cut(s) 155, 227, 278
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PpuMI RGGWCCY 1 cut(s) 359
Psp5II RGGWCCY 1 cut(s) 359
PspEI GGTNACC 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 118, 360, 361
PspPI GGNCC 1 cut(s) 359
PspPPI RGGWCCY 1 cut(s) 359
PsuI RGATCY 2 cut(s) 107, 395
SalI GTCGAC 2 cut(s) 47, 80
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 3 cut(s) 154, 226, 277
Sau3AI GATC 5 cut(s) 107, 319, 332, 350, 395
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 1 cut(s) 185
SetI ASST 7 cut(s) 130, 136, 148, 166, 187, 199, 221
SfaNI GCATC 1 cut(s) 389
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 359
SsiI CCGC 1 cut(s) 153
SspMI CTAG 2 cut(s) 216, 393
TaaI ACNGT 2 cut(s) 65, 140
TaiI ACGT 1 cut(s) 187
TaqI TCGA 3 cut(s) 48, 81, 331
TauI GCSGC 1 cut(s) 156
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TseFI GTSAC 2 cut(s) 159, 188
TseI GCWGC 2 cut(s) 225, 276
Tsp45I GTSAC 2 cut(s) 159, 188
VpaK11BI GGWCC 1 cut(s) 359
XbaI TCTAGA 1 cut(s) 392
XmiI GTMKAC 2 cut(s) 48, 81
XspI CTAG 2 cut(s) 216, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.