AT3G45740

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001386
Physical Location & Seq
Forward (+)
16790761 .. 16793540
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G45740.2

Sequence Viewer

Length: 1131 bp
ATGAATCATCTTCTAATCTTAGCGAGAAACTCGCTGCGGCGGCGGAATTTTAATCTCCGACCACCGTCTCGTTCCTTCAGTAGCACCATCTCACCGGAATCGAATCGCTCGTCTTTTGGCATAGCATTCGATATCGATGGAGTTATTCTTCTTGGTTCCTCTCCCGTTGGTGGCTCTCCTAGCGCTCTCCGACGTCTCTACGATGATTCCGGTGCTTTGAAGATACCTTTCTTATTCTTGACTAATGGAGGTGGCTTGCCTGAATCTAAACGGGCTTCTGAGATGAGTCATTTATTAGGTGTTCAAGTTTCTCCATTGCAGGTGATACAAGCACATTCACCCTTTCGGAAGCTAGTAAATAGGTTTGAAAATGAGCTTGTTGTTGCAGCTGGAAAAGGGGAACCTGCCGCGGTGATGTCTAATTATGGTTTCAAAAACGTCATTTCAATGGACGAATATGCCTCTTACTTTGACAACATTGATCCCTTGGCGCCATATAAGAAATTGATGTTCAGGCAGGATGGTCACAAGGAGCTTAGATCAAGAGAAGATGTTCTATCCCAAAGAGTTCAGGCAGCATTTATAGTTAGTGATCCAGTTGATTGGAGCCGAGACATCCAGGTTCTTTGTGACATTTTACGAACTGGAGGACTTCCTGGGAAAGAAATTGGACCTCAACCTCATTTGTACATTGCCAATGACGATTTAGACTACCAGACTGAGTTTCCTACTGAACGTCTTGGCATGGGAGCTTTTAGAATCGCGTTAGAATCAATCTTCAACAGAATTCATGAAAAACCTCTAGAGTACACATCATTTGGCAAACCAAATCCATTTGTGTTCAAAAACGCAGAAGATGTTCTGAAAGAAATCGCTACATCCCCTTATTCTTCAAACCAAGGAAGTCACCACTTCAAGACACTATATATGATCGGGGACAACCCTAAAATCGACATCAGAGGCGCACGACAGGCTGGGACTCCTTGGTTTTCAATATTAACGAGAACCGGAGTTTTCAAAGGAAAAGATAACCATTCCGAGTTTCCAGCTGATCTGGTCGTTGACACCGTCGAGGAAGCAGTTGATTTCATTTTGACAAGAGAGTCCGCCAAGCAAAGCTTACTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

376

Amino Acids

41.91

Weight (kDa)

6.77

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_like PF13242 179 - 266 2.6e-13 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 310
AasI GACNNNNNNGTC 1 cut(s) 1102
AatII GACGTC 1 cut(s) 196
Acc36I ACCTGC 2 cut(s) 310, 412
AccB1I GGYRCC 1 cut(s) 490
AccII CGCG 2 cut(s) 410, 764
AciI CCGC 6 cut(s) 37, 40, 43, 408, 410, 1107
AclWI GGATC 2 cut(s) 476, 587
AcsI RAATTY 2 cut(s) 46, 786
AcuI CTGAAG 1 cut(s) 61
AcyI GRCGYC 2 cut(s) 193, 491
AfaI GTAC 2 cut(s) 689, 809
AfeI AGCGCT 1 cut(s) 184
AfiI CCNNNNNNNGG 1 cut(s) 170
AjnI CCWGG 2 cut(s) 618, 655
AluBI AGCT 7 cut(s) 352, 376, 389, 535, 752, 1049, 1119
AluI AGCT 7 cut(s) 352, 376, 389, 535, 752, 1049, 1119
Alw26I GTCTC 3 cut(s) 72, 200, 606
AlwI GGATC 2 cut(s) 476, 587
Aor51HI AGCGCT 1 cut(s) 184
ApeKI GCWGC 3 cut(s) 34, 386, 575
ApoI RAATTY 2 cut(s) 46, 786
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
Asp700I GAANNNNTTC 2 cut(s) 552, 858
AspLEI GCGC 3 cut(s) 185, 493, 965
AspS9I GGNCC 1 cut(s) 671
AsuHPI GGTGA 5 cut(s) 84, 330, 334, 424, 899
AvaII GGWCC 1 cut(s) 671
BanI GGYRCC 1 cut(s) 490
BbvI GCAGC 3 cut(s) 21, 398, 587
BccI CCATC 3 cut(s) 95, 131, 515
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 2 cut(s) 620, 657
BcoDI GTCTC 3 cut(s) 72, 200, 606
BfaI CTAG 3 cut(s) 180, 353, 803
BfoI RGCGCY 2 cut(s) 186, 494
BfuAI ACCTGC 2 cut(s) 310, 412
BisI GCNGC 6 cut(s) 35, 38, 41, 387, 408, 576
BlsI GCNGC 6 cut(s) 36, 39, 42, 388, 409, 577
Bme1390I CCNGG 2 cut(s) 620, 657
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 4 cut(s) 157, 402, 492, 608
BmrFI CCNGG 2 cut(s) 620, 657
BoxI GACNNNNGTC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 666
Bsa29I ATCGAT 1 cut(s) 135
BsaHI GRCGYC 2 cut(s) 193, 491
BsaJI CCNNGG 5 cut(s) 408, 486, 656, 898, 983
BsaWI WCCGGW 3 cut(s) 94, 209, 1007
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 596, 649
Bse3DI GCAATG 2 cut(s) 314, 690
BseBI CCWGG 2 cut(s) 620, 657
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 5 cut(s) 408, 486, 656, 898, 983
BseGI GGATG 3 cut(s) 526, 615, 878
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 2 cut(s) 314, 690
BseMII CTCAG 2 cut(s) 270, 711
BseNI ACTGG 2 cut(s) 596, 649
BseXI GCAGC 3 cut(s) 21, 398, 587
BseYI CCCAGC 1 cut(s) 974
Bsh1236I CGCG 2 cut(s) 410, 764
BshNI GGYRCC 1 cut(s) 490
BshVI ATCGAT 1 cut(s) 135
BsiSI CCGG 3 cut(s) 95, 210, 1008
BslFI GGGAC 2 cut(s) 950, 991
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 3 cut(s) 72, 200, 606
BsmBI CGTCTC 2 cut(s) 72, 200
BsmFI GGGAC 2 cut(s) 950, 991
BsmI GAATGC 1 cut(s) 125
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 5 cut(s) 481, 539, 592, 930, 1051
BspACI CCGC 6 cut(s) 37, 40, 43, 408, 410, 1107
BspCNI CTCAG 2 cut(s) 271, 712
BspDI ATCGAT 1 cut(s) 135
BspFNI CGCG 2 cut(s) 410, 764
BspHI TCATGA 2 cut(s) 790, 1127
BspLI GGNNCC 4 cut(s) 157, 402, 492, 608
BspMI ACCTGC 2 cut(s) 310, 412
BspPI GGATC 2 cut(s) 476, 587
BspT107I GGYRCC 1 cut(s) 490
BsrDI GCAATG 2 cut(s) 314, 690
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 596, 649
BssECI CCNNGG 5 cut(s) 408, 486, 656, 898, 983
BssMI GATC 5 cut(s) 481, 539, 592, 930, 1051
BssNI GRCGYC 2 cut(s) 193, 491
BssT1I CCWWGG 3 cut(s) 486, 898, 983
Bst2UI CCWGG 2 cut(s) 620, 657
Bst4CI ACNGT 3 cut(s) 66, 1069, 1125
BstACI GRCGYC 2 cut(s) 193, 491
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 4 cut(s) 19, 279, 536, 720
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 3 cut(s) 526, 615, 878
BstFNI CGCG 2 cut(s) 410, 764
BstH2I RGCGCY 2 cut(s) 186, 494
BstHHI GCGC 3 cut(s) 185, 493, 965
BstKTI GATC 5 cut(s) 484, 542, 595, 933, 1054
BstMAI GTCTC 3 cut(s) 72, 200, 606
BstMBI GATC 5 cut(s) 481, 539, 592, 930, 1051
BstMWI GCNNNNNNNGC 3 cut(s) 40, 180, 971
BstNI CCWGG 2 cut(s) 620, 657
BstPAI GACNNNNGTC 1 cut(s) 64
BstSCI CCNGG 2 cut(s) 618, 655
BstUI CGCG 2 cut(s) 410, 764
BstV1I GCAGC 3 cut(s) 21, 398, 587
BstXI CCANNNNNNTGG 1 cut(s) 603
Bsu15I ATCGAT 1 cut(s) 135
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 526, 615, 878
BveI ACCTGC 2 cut(s) 310, 412
Cac8I GCNNGC 1 cut(s) 257
CciI TCATGA 2 cut(s) 790, 1127
CfoI GCGC 3 cut(s) 185, 493, 965
Cfr13I GGNCC 1 cut(s) 671
Cfr42I CCGCGG 1 cut(s) 411
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 688, 808
CviAII CATG 3 cut(s) 745, 791, 1128
CviQI GTAC 2 cut(s) 688, 808
DdeI CTNAG 4 cut(s) 19, 279, 536, 720
DinI GGCGCC 1 cut(s) 492
DpnI GATC 5 cut(s) 483, 541, 594, 932, 1053
DpnII GATC 5 cut(s) 481, 539, 592, 930, 1051
DrdI GACNNNNNNGTC 1 cut(s) 1102
DseDI GACNNNNNNGTC 1 cut(s) 1102
EciI GGCGGA 2 cut(s) 58, 1096
Eco130I CCWWGG 3 cut(s) 486, 898, 983
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 671
Eco47III AGCGCT 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 786
EcoRII CCWGG 2 cut(s) 618, 655
EcoRV GATATC 1 cut(s) 133
EcoT14I CCWWGG 3 cut(s) 486, 898, 983
EgeI GGCGCC 1 cut(s) 492
EheI GGCGCC 1 cut(s) 492
ErhI CCWWGG 3 cut(s) 486, 898, 983
Esp3I CGTCTC 2 cut(s) 72, 200
FaeI CATG 3 cut(s) 748, 794, 1131
FalI AAGNNNNNCTT 1 cut(s) 1103
FaqI GGGAC 2 cut(s) 950, 991
FatI CATG 3 cut(s) 744, 790, 1127
Fnu4HI GCNGC 6 cut(s) 35, 38, 41, 387, 408, 576
FokI GGATG 3 cut(s) 533, 602, 865
Fsp4HI GCNGC 6 cut(s) 35, 38, 41, 387, 408, 576
FspBI CTAG 3 cut(s) 180, 353, 803
GlaI GCGC 3 cut(s) 184, 492, 964
GluI GCNGC 6 cut(s) 35, 38, 41, 387, 408, 576
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 1 cut(s) 666
HaeII RGCGCY 2 cut(s) 186, 494
HapII CCGG 3 cut(s) 95, 210, 1008
HhaI GCGC 3 cut(s) 185, 493, 965
Hin1I GRCGYC 2 cut(s) 193, 491
Hin1II CATG 3 cut(s) 748, 794, 1131
Hin6I GCGC 3 cut(s) 183, 491, 963
HinP1I GCGC 3 cut(s) 183, 491, 963
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HindIII AAGCTT 1 cut(s) 1117
HpaII CCGG 3 cut(s) 95, 210, 1008
HphI GGTGA 5 cut(s) 84, 330, 334, 424, 899
Hpy166II GTNNAC 2 cut(s) 810, 1063
Hpy188I TCNGA 7 cut(s) 59, 191, 280, 348, 864, 959, 1039
Hpy188III TCNNGA 6 cut(s) 238, 543, 791, 803, 916, 1128
Hpy8I GTNNAC 2 cut(s) 810, 1063
Hpy99I CGWCG 2 cut(s) 195, 1073
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 3 cut(s) 66, 1069, 1125
HpyCH4IV ACGT 3 cut(s) 193, 438, 736
HpyCH4V TGCA 2 cut(s) 319, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 180, 971
HpyF3I CTNAG 4 cut(s) 19, 279, 536, 720
HpySE526I ACGT 3 cut(s) 193, 438, 736
Hsp92I GRCGYC 2 cut(s) 193, 491
Hsp92II CATG 3 cut(s) 748, 794, 1131
HspAI GCGC 3 cut(s) 183, 491, 963
KasI GGCGCC 1 cut(s) 490
KspI CCGCGG 1 cut(s) 411
Kzo9I GATC 5 cut(s) 481, 539, 592, 930, 1051
LmnI GCTCC 3 cut(s) 532, 606, 749
Lsp1109I GCAGC 3 cut(s) 21, 398, 587
MaeI CTAG 3 cut(s) 180, 353, 803
MaeII ACGT 3 cut(s) 193, 438, 736
MaeIII GTNAC 3 cut(s) 524, 629, 905
MalI GATC 5 cut(s) 483, 541, 594, 932, 1053
MboI GATC 5 cut(s) 481, 539, 592, 930, 1051
MboII GAAGA 6 cut(s) 140, 232, 560, 769, 866, 882
MluCI AATT 5 cut(s) 46, 421, 503, 666, 786
Mly113I GGCGCC 1 cut(s) 491
MlyI GAGTC 3 cut(s) 295, 973, 1112
MmeI TCCRAC 2 cut(s) 82, 214
MnlI CCTC 9 cut(s) 169, 242, 472, 641, 684, 690, 810, 953, 1066
MroXI GAANNNNTTC 2 cut(s) 552, 858
MseI TTAA 2 cut(s) 51, 998
MslI CAYNNNNRTG 1 cut(s) 446
MspA1I CMGCKG 3 cut(s) 389, 410, 1049
MspI CCGG 3 cut(s) 95, 210, 1008
MspR9I CCNGG 2 cut(s) 620, 657
Mva1269I GAATGC 1 cut(s) 125
MvaI CCWGG 2 cut(s) 620, 657
MvnI CGCG 2 cut(s) 410, 764
MwoI GCNNNNNNNGC 3 cut(s) 40, 180, 971
NarI GGCGCC 1 cut(s) 491
NdeII GATC 5 cut(s) 481, 539, 592, 930, 1051
NlaIII CATG 3 cut(s) 748, 794, 1131
NlaIV GGNNCC 4 cut(s) 157, 402, 492, 608
NmeAIII GCCGAG 1 cut(s) 635
NmuCI GTSAC 3 cut(s) 524, 629, 905
PagI TCATGA 2 cut(s) 790, 1127
PaqCI CACCTGC 1 cut(s) 310
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 1065
PctI GAATGC 1 cut(s) 125
PdmI GAANNNNTTC 2 cut(s) 552, 858
PfeI GAWTC 7 cut(s) 4, 98, 103, 206, 263, 759, 770
PflFI GACNNNGTC 1 cut(s) 1067
PkrI GCNGC 6 cut(s) 36, 39, 42, 388, 409, 577
PleI GAGTC 3 cut(s) 294, 973, 1111
PluTI GGCGCC 1 cut(s) 494
PpsI GAGTC 3 cut(s) 294, 973, 1111
PshAI GACNNNNGTC 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 618, 655
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 2 cut(s) 618, 655
PspN4I GGNNCC 4 cut(s) 157, 402, 492, 608
PspPI GGNCC 1 cut(s) 671
PsyI GACNNNGTC 1 cut(s) 1067
PvuII CAGCTG 2 cut(s) 389, 1049
RsaI GTAC 2 cut(s) 689, 809
RsaNI GTAC 2 cut(s) 688, 808
RseI CAYNNNNRTG 1 cut(s) 446
SacII CCGCGG 1 cut(s) 411
SaqAI TTAA 2 cut(s) 51, 998
SatI GCNGC 6 cut(s) 35, 38, 41, 387, 408, 576
Sau3AI GATC 5 cut(s) 481, 539, 592, 930, 1051
Sau96I GGNCC 1 cut(s) 671
SchI GAGTC 3 cut(s) 295, 973, 1112
ScrFI CCNGG 2 cut(s) 620, 657
SfoI GGCGCC 1 cut(s) 492
Sfr303I CCGCGG 1 cut(s) 411
SgrBI CCGCGG 1 cut(s) 411
SinI GGWCC 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 5 cut(s) 46, 421, 503, 666, 786
SsiI CCGC 6 cut(s) 37, 40, 43, 408, 410, 1107
SspDI GGCGCC 1 cut(s) 490
SspI AATATT 1 cut(s) 996
SspMI CTAG 3 cut(s) 180, 353, 803
StyD4I CCNGG 2 cut(s) 618, 655
StyI CCWWGG 3 cut(s) 486, 898, 983
TaaI ACNGT 3 cut(s) 66, 1069, 1125
TaiI ACGT 3 cut(s) 196, 441, 739
TaqI TCGA 5 cut(s) 101, 129, 135, 951, 1071
TasI AATT 5 cut(s) 46, 421, 503, 666, 786
TatI WGTACW 2 cut(s) 687, 807
TauI GCSGC 3 cut(s) 40, 43, 410
TfiI GAWTC 7 cut(s) 4, 98, 103, 206, 263, 759, 770
Tru1I TTAA 2 cut(s) 51, 998
Tru9I TTAA 2 cut(s) 51, 998
TseFI GTSAC 3 cut(s) 524, 629, 905
TseI GCWGC 3 cut(s) 34, 386, 575
Tsp45I GTSAC 3 cut(s) 524, 629, 905
TspDTI ATGAA 4 cut(s) 17, 779, 807, 1078
Tth111I GACNNNGTC 1 cut(s) 1067
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 2 cut(s) 46, 786
XbaI TCTAGA 1 cut(s) 802
XmnI GAANNNNTTC 2 cut(s) 552, 858
XspI CTAG 3 cut(s) 180, 353, 803
ZraI GACGTC 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.