RLG00000027485

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001386
Physical Location & Seq
Reverse (-)
12098606 .. 12102798
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027485

Sequence Viewer

Length: 1128 bp
ATGTCGCCGATGAGATACCGGACGGCGTTTAGGCTTTCCCGGAGCAGAAACCGACTGCCGTTTCCGTTGCGGTCGCGTCCGCACTCCTCTGAGTCTCGGATTCAATGCAGGCCTGAACCACGGTCTTCGTTTGGAATTGCGTTTGACATTGACGGAGTTATTCTCGGCGGCCGTGCTCCGATCGGCGGGTCTCCTCGAGCTTTGAGGAAGCTGTACGGAGCTTCAGGGGGTGGCATCCCAGAGTCGAGACGAGCTGCTGAACTAAGTGAAGTTCTGGGGGTCAATATCCTGCCTTCTCAGGTTGTACAGGGTCATACTCCTTTTAGAAACTTGTTGAGGAGATATGAGAATGAATTGATTGTTGCTGTTGGAAAAGGGGAACCAGCTCTTGTAATGTCAGAGTATGGTTTCAAAAAAGTTCTCTCATTAGATGAGTATGCATCCTACTTCAAAGGTATTGACCCGGTAGCTCAATATAAGATGTGGAGAACTAAGCAGGCATTGGATTGCAGTAGCCACTTGAAGGAATCGGTGCCAAGATATGATGTATACTCTGACAGGGTTAGTGCTGCTTTTGTCGTTAGTGATCCTGTAGATTGGGGCCGGGACATTCAGGTTCTCTGTGACATTTTAAGATCTGGAGGGATTCCTGGATGGGAGAATGGCAATCAGCCACCCCTTTATTTTGCCGCAGATGACCTTGAATATCAGGCTGCATTTCCTTCTCAGAGACTTGGAATGGGTGCTTTCAGAATTGCACTAGAAAGCATCTTCAACAGAATTCACCATAGTACTTTGAAATATGTATCTTTCGGGAAGCCCAATCCGTTTGTATTTAAGAACACTGAAGATCTATTGGCAGAGTTTCAACCATCTCAATTTGATGATCATATTTCAGAAAGTGGATGCTCTCAGTCACATGATTTCAAAACCCTATATATGATCGGAGACAACCCTTTGGTTGATGTCAAAGGCGCTCAACAGGCCGGACATCCCTGGTTTTCTATTTTGACAAGGACAGGTGTTTTTAAGGGAAAAGATAATCATAAGGAGTTTCCAGCAGATATGGTTGTGGATACTGTAGAAGAGGCAGTGGACTTCATTCTGAAAAGAGAAGGAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

376

Amino Acids

41.96

Weight (kDa)

8.24

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 46 - 138 2e-09 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 271 - 364 9.3e-12 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 532
AccI GTMKAC 1 cut(s) 549
AccII CGCG 1 cut(s) 76
AciI CCGC 5 cut(s) 70, 80, 168, 186, 690
AclWI GGATC 1 cut(s) 581
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 780
AcuI CTGAAG 2 cut(s) 207, 867
AfaI GTAC 3 cut(s) 215, 306, 793
AfiI CCNNNNNNNGG 2 cut(s) 185, 523
AgsI TTSAA 9 cut(s) 104, 412, 451, 523, 704, 775, 799, 869, 928
AjnI CCWGG 2 cut(s) 649, 995
AluBI AGCT 6 cut(s) 200, 211, 221, 254, 386, 470
AluI AGCT 6 cut(s) 200, 211, 221, 254, 386, 470
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 5 cut(s) 99, 195, 241, 724, 942
AlwI GGATC 1 cut(s) 581
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 4 cut(s) 110, 169, 601, 984
ApeKI GCWGC 3 cut(s) 254, 569, 713
ApoI RAATTY 1 cut(s) 780
AspLEI GCGC 1 cut(s) 977
AspS9I GGNCC 1 cut(s) 601
AsuC2I CCSGG 3 cut(s) 40, 464, 605
AsuHPI GGTGA 1 cut(s) 776
AvaI CYCGRG 1 cut(s) 195
BanI GGYRCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 241, 556, 700
BccI CCATC 2 cut(s) 648, 880
BceAI ACGGC 3 cut(s) 39, 43, 156
BciT130I CCWGG 2 cut(s) 651, 997
BciVI GTATCC 1 cut(s) 1069
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 3 cut(s) 40, 464, 605
BcoDI GTCTC 5 cut(s) 99, 195, 241, 724, 942
BfaI CTAG 1 cut(s) 761
BfmI CTRYAG 2 cut(s) 591, 1080
BfoI RGCGCY 1 cut(s) 978
BfuI GTATCC 1 cut(s) 1069
BglII AGATCT 2 cut(s) 635, 850
BisI GCNGC 5 cut(s) 169, 255, 570, 690, 714
BlsI GCNGC 5 cut(s) 170, 256, 571, 691, 715
BmcAI AGTACT 1 cut(s) 793
Bme1390I CCNGG 5 cut(s) 40, 464, 605, 651, 997
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 3 cut(s) 381, 534, 602
BmrFI CCNGG 5 cut(s) 40, 464, 605, 651, 997
BmsI GCATC 4 cut(s) 243, 449, 777, 896
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpmI CTGGAG 1 cut(s) 660
BpuMI CCSGG 3 cut(s) 40, 464, 605
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 119, 995
BsaWI WCCGGW 1 cut(s) 18
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 523
BseBI CCWGG 2 cut(s) 651, 997
BseDI CCNNGG 2 cut(s) 119, 995
BseGI GGATG 5 cut(s) 234, 440, 659, 911, 991
BseLI CCNNNNNNNGG 2 cut(s) 185, 523
BseMII CTCAG 4 cut(s) 81, 311, 740, 926
BseRI GAGGAG 3 cut(s) 76, 183, 352
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 241, 556, 700
Bsh1236I CGCG 1 cut(s) 76
Bsh1285I CGRYCG 3 cut(s) 74, 172, 183
BshFI GGCC 4 cut(s) 112, 171, 603, 986
BshNI GGYRCC 1 cut(s) 532
BsiEI CGRYCG 3 cut(s) 74, 172, 183
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 5 cut(s) 19, 40, 464, 604, 987
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 2 cut(s) 185, 523
BsmAI GTCTC 5 cut(s) 99, 195, 241, 724, 942
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 4 cut(s) 112, 171, 603, 986
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 178
Bsp1407I TGTACA 1 cut(s) 304
Bsp143I GATC 6 cut(s) 180, 586, 635, 850, 886, 942
BspACI CCGC 5 cut(s) 70, 80, 168, 186, 690
BspANI GGCC 4 cut(s) 112, 171, 603, 986
BspCNI CTCAG 4 cut(s) 82, 310, 739, 925
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 381, 534, 602
BspPI GGATC 1 cut(s) 581
BspT107I GGYRCC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 195
BsrGI TGTACA 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 119, 995
BssMI GATC 6 cut(s) 180, 586, 635, 850, 886, 942
BssNAI GTATAC 1 cut(s) 550
Bst1107I GTATAC 1 cut(s) 550
Bst2UI CCWGG 2 cut(s) 651, 997
Bst4CI ACNGT 2 cut(s) 123, 1081
Bst6I CTCTTC 1 cut(s) 1080
BstAUI TGTACA 1 cut(s) 304
BstC8I GCNNGC 2 cut(s) 110, 498
BstDEI CTNAG 7 cut(s) 90, 263, 297, 492, 726, 912, 1125
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 5 cut(s) 234, 440, 659, 911, 991
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 978
BstHHI GCGC 1 cut(s) 977
BstKTI GATC 6 cut(s) 183, 589, 638, 853, 889, 945
BstMAI GTCTC 5 cut(s) 99, 195, 241, 724, 942
BstMBI GATC 6 cut(s) 180, 586, 635, 850, 886, 942
BstMCI CGRYCG 3 cut(s) 74, 172, 183
BstMWI GCNNNNNNNGC 1 cut(s) 983
BstNI CCWGG 2 cut(s) 651, 997
BstSCI CCNGG 5 cut(s) 38, 462, 603, 649, 995
BstSFI CTRYAG 2 cut(s) 591, 1080
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 3 cut(s) 241, 556, 700
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 2 cut(s) 635, 850
BstYI RGATCY 2 cut(s) 635, 850
BstZ17I GTATAC 1 cut(s) 550
BstZI CGGCCG 1 cut(s) 169
BsuI GTATCC 1 cut(s) 1069
BsuRI GGCC 4 cut(s) 112, 171, 603, 986
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 5 cut(s) 234, 440, 659, 911, 991
BtsI GCAGTG 1 cut(s) 1098
BtsIMutI CAGTG 2 cut(s) 843, 1098
Cac8I GCNNGC 2 cut(s) 110, 498
CfoI GCGC 1 cut(s) 977
Cfr13I GGNCC 1 cut(s) 601
CseI GACGC 1 cut(s) 65
Csp6I GTAC 3 cut(s) 214, 305, 792
CviAII CATG 1 cut(s) 920
CviQI GTAC 3 cut(s) 214, 305, 792
DdeI CTNAG 7 cut(s) 90, 263, 297, 492, 726, 912, 1125
DpnI GATC 6 cut(s) 182, 588, 637, 852, 888, 944
DpnII GATC 6 cut(s) 180, 586, 635, 850, 886, 942
EaeI YGGCCR 1 cut(s) 169
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 1080
EarI CTCTTC 1 cut(s) 1080
EclXI CGGCCG 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 195
Eco52I CGGCCG 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 207, 867
Eco88I CYCGRG 1 cut(s) 195
EcoRI GAATTC 1 cut(s) 780
EcoRII CCWGG 2 cut(s) 649, 995
EcoT22I ATGCAT 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 1 cut(s) 923
FaqI GGGAC 1 cut(s) 620
FatI CATG 1 cut(s) 919
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 1 cut(s) 886
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 5 cut(s) 169, 255, 570, 690, 714
FokI GGATG 5 cut(s) 221, 427, 666, 918, 978
Fsp4HI GCNGC 5 cut(s) 169, 255, 570, 690, 714
FspBI CTAG 1 cut(s) 761
GlaI GCGC 1 cut(s) 976
GluI GCNGC 5 cut(s) 169, 255, 570, 690, 714
GsuI CTGGAG 1 cut(s) 660
HaeII RGCGCY 1 cut(s) 978
HaeIII GGCC 4 cut(s) 112, 171, 603, 986
HapII CCGG 5 cut(s) 19, 40, 464, 604, 987
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 977
Hin1II CATG 1 cut(s) 923
Hin6I GCGC 1 cut(s) 975
HinP1I GCGC 1 cut(s) 975
HinfI GANTC 5 cut(s) 92, 100, 242, 527, 646
HpaII CCGG 5 cut(s) 19, 40, 464, 604, 987
HphI GGTGA 1 cut(s) 776
Hpy166II GTNNAC 2 cut(s) 550, 1096
Hpy188III TCNNGA 3 cut(s) 246, 639, 814
Hpy8I GTNNAC 2 cut(s) 550, 1096
HpyAV CCTTC 4 cut(s) 303, 517, 732, 1109
HpyCH4III ACNGT 2 cut(s) 123, 1081
HpyCH4V TGCA 5 cut(s) 108, 440, 510, 716, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 983
HpyF3I CTNAG 7 cut(s) 90, 263, 297, 492, 726, 912, 1125
Hsp92II CATG 1 cut(s) 923
HspAI GCGC 1 cut(s) 975
Ksp22I TGATCA 1 cut(s) 886
Kzo9I GATC 6 cut(s) 180, 586, 635, 850, 886, 942
LmnI GCTCC 3 cut(s) 42, 181, 218
Lsp1109I GCAGC 3 cut(s) 241, 556, 700
LweI GCATC 4 cut(s) 243, 449, 777, 896
MaeI CTAG 1 cut(s) 761
MaeIII GTNAC 2 cut(s) 623, 915
MalI GATC 6 cut(s) 182, 588, 637, 852, 888, 944
MboI GATC 6 cut(s) 180, 586, 635, 850, 886, 942
MboII GAAGA 4 cut(s) 117, 763, 860, 1097
MflI RGATCY 2 cut(s) 635, 850
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 5 cut(s) 135, 353, 753, 780, 878
MlyI GAGTC 2 cut(s) 101, 251
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 6 cut(s) 97, 198, 204, 330, 635, 1081
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 3 cut(s) 632, 837, 1029
MspI CCGG 5 cut(s) 19, 40, 464, 604, 987
MspR9I CCNGG 5 cut(s) 40, 464, 605, 651, 997
MvaI CCWGG 2 cut(s) 651, 997
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 1 cut(s) 983
NciI CCSGG 3 cut(s) 40, 464, 605
NdeII GATC 6 cut(s) 180, 586, 635, 850, 886, 942
NlaIII CATG 1 cut(s) 923
NlaIV GGNNCC 3 cut(s) 381, 534, 602
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 623, 915
NsiI ATGCAT 1 cut(s) 442
PaeR7I CTCGAG 1 cut(s) 195
PceI AGGCCT 1 cut(s) 112
PfeI GAWTC 3 cut(s) 100, 527, 646
PfoI TCCNGGA 2 cut(s) 38, 649
PkrI GCNGC 5 cut(s) 170, 256, 571, 691, 715
Ple19I CGATCG 1 cut(s) 183
PleI GAGTC 2 cut(s) 100, 250
PpsI GAGTC 2 cut(s) 100, 250
Psp6I CCWGG 2 cut(s) 649, 995
PspGI CCWGG 2 cut(s) 649, 995
PspN4I GGNNCC 3 cut(s) 381, 534, 602
PspPI GGNCC 1 cut(s) 601
PspXI VCTCGAGB 1 cut(s) 195
PsuI RGATCY 2 cut(s) 635, 850
PvuI CGATCG 1 cut(s) 183
RsaI GTAC 3 cut(s) 215, 306, 793
RsaNI GTAC 3 cut(s) 214, 305, 792
SaqAI TTAA 3 cut(s) 632, 837, 1029
SatI GCNGC 5 cut(s) 169, 255, 570, 690, 714
Sau3AI GATC 6 cut(s) 180, 586, 635, 850, 886, 942
Sau96I GGNCC 1 cut(s) 601
ScaI AGTACT 1 cut(s) 793
SchI GAGTC 2 cut(s) 101, 251
ScrFI CCNGG 5 cut(s) 40, 464, 605, 651, 997
SduI GDGCHC 1 cut(s) 178
SfaNI GCATC 4 cut(s) 243, 449, 777, 896
SfcI CTRYAG 2 cut(s) 591, 1080
Sfr274I CTCGAG 1 cut(s) 195
SlaI CTCGAG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 5 cut(s) 135, 353, 753, 780, 878
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 5 cut(s) 70, 80, 168, 186, 690
SspMI CTAG 1 cut(s) 761
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 5 cut(s) 38, 462, 603, 649, 995
TaaI ACNGT 2 cut(s) 123, 1081
TaqI TCGA 2 cut(s) 196, 245
TasI AATT 5 cut(s) 135, 353, 753, 780, 878
TatI WGTACW 2 cut(s) 304, 791
TauI GCSGC 2 cut(s) 171, 692
TfiI GAWTC 3 cut(s) 100, 527, 646
Tru1I TTAA 3 cut(s) 632, 837, 1029
Tru9I TTAA 3 cut(s) 632, 837, 1029
TscAI CASTG 2 cut(s) 850, 1098
TseFI GTSAC 2 cut(s) 623, 915
TseI GCWGC 3 cut(s) 254, 569, 713
Tsp45I GTSAC 2 cut(s) 623, 915
TspDTI ATGAA 2 cut(s) 366, 1090
TspGWI ACGGA 4 cut(s) 54, 168, 231, 816
TspRI CASTG 2 cut(s) 850, 1098
XapI RAATTY 1 cut(s) 780
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 549
XspI CTAG 1 cut(s) 761
ZrmI AGTACT 1 cut(s) 793
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.