Haloacid dehalogenase-like hydrolase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 18, 98, 183 |
| AcoI | YGGCCR | 1 cut(s) | 157 |
| AgsI | TTSAA | 2 cut(s) | 197, 268 |
| AjnI | CCWGG | 1 cut(s) | 142 |
| AluBI | AGCT | 1 cut(s) | 62 |
| AluI | AGCT | 1 cut(s) | 62 |
| Alw26I | GTCTC | 1 cut(s) | 217 |
| AoxI | GGCC | 1 cut(s) | 157 |
| ApeKI | GCWGC | 2 cut(s) | 62, 206 |
| BalI | TGGCCA | 1 cut(s) | 159 |
| BbvI | GCAGC | 2 cut(s) | 49, 193 |
| BccI | CCATC | 2 cut(s) | 141, 168 |
| BciT130I | CCWGG | 1 cut(s) | 144 |
| BcoDI | GTCTC | 1 cut(s) | 217 |
| BfaI | CTAG | 1 cut(s) | 254 |
| BglII | AGATCT | 2 cut(s) | 128, 272 |
| BisI | GCNGC | 3 cut(s) | 63, 183, 207 |
| BlpI | GCTNAGC | 1 cut(s) | 14 |
| BlsI | GCNGC | 3 cut(s) | 64, 184, 208 |
| Bme1390I | CCNGG | 1 cut(s) | 144 |
| BmrFI | CCNGG | 1 cut(s) | 144 |
| BmsI | GCATC | 1 cut(s) | 51 |
| BpmI | CTGGAG | 1 cut(s) | 153 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 14 |
| BseBI | CCWGG | 1 cut(s) | 144 |
| BseGI | GGATG | 2 cut(s) | 42, 152 |
| BseMII | CTCAG | 1 cut(s) | 233 |
| BseXI | GCAGC | 2 cut(s) | 49, 193 |
| BshFI | GGCC | 1 cut(s) | 159 |
| BsmAI | GTCTC | 1 cut(s) | 217 |
| BsmI | GAATGC | 1 cut(s) | 239 |
| BsnI | GGCC | 1 cut(s) | 159 |
| Bsp143I | GATC | 2 cut(s) | 128, 272 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 14 |
| BspACI | CCGC | 3 cut(s) | 18, 98, 183 |
| BspANI | GGCC | 1 cut(s) | 159 |
| BspCNI | CTCAG | 1 cut(s) | 232 |
| BssMI | GATC | 2 cut(s) | 128, 272 |
| Bst2UI | CCWGG | 1 cut(s) | 144 |
| BstDEI | CTNAG | 2 cut(s) | 14, 219 |
| BstF5I | GGATG | 2 cut(s) | 42, 152 |
| BstKTI | GATC | 2 cut(s) | 131, 275 |
| BstMAI | GTCTC | 1 cut(s) | 217 |
| BstMBI | GATC | 2 cut(s) | 128, 272 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 39 |
| BstNI | CCWGG | 1 cut(s) | 144 |
| BstSCI | CCNGG | 1 cut(s) | 142 |
| BstV1I | GCAGC | 2 cut(s) | 49, 193 |
| BstX2I | RGATCY | 2 cut(s) | 128, 272 |
| BstYI | RGATCY | 2 cut(s) | 128, 272 |
| BsuRI | GGCC | 1 cut(s) | 159 |
| BtsCI | GGATG | 2 cut(s) | 42, 152 |
| CviJI | RGCY | 5 cut(s) | 13, 62, 159, 166, 206 |
| CviKI_1 | RGCY | 5 cut(s) | 13, 62, 159, 166, 206 |
| DdeI | CTNAG | 2 cut(s) | 14, 219 |
| DpnI | GATC | 2 cut(s) | 130, 274 |
| DpnII | GATC | 2 cut(s) | 128, 272 |
| EaeI | YGGCCR | 1 cut(s) | 157 |
| EcoRII | CCWGG | 1 cut(s) | 142 |
| FaiI | YATR | 2 cut(s) | 201, 263 |
| FauI | CCCGC | 1 cut(s) | 105 |
| Fnu4HI | GCNGC | 3 cut(s) | 63, 183, 207 |
| FokI | GGATG | 2 cut(s) | 29, 159 |
| Fsp4HI | GCNGC | 3 cut(s) | 63, 183, 207 |
| FspBI | CTAG | 1 cut(s) | 254 |
| GluI | GCNGC | 3 cut(s) | 63, 183, 207 |
| GsuI | CTGGAG | 1 cut(s) | 153 |
| HaeIII | GGCC | 1 cut(s) | 159 |
| HinfI | GANTC | 3 cut(s) | 50, 87, 139 |
| Hpy166II | GTNNAC | 1 cut(s) | 286 |
| Hpy188I | TCNGA | 3 cut(s) | 222, 245, 293 |
| Hpy188III | TCNNGA | 1 cut(s) | 132 |
| Hpy8I | GTNNAC | 1 cut(s) | 286 |
| HpyAV | CCTTC | 1 cut(s) | 225 |
| HpyCH4V | TGCA | 3 cut(s) | 209, 239, 251 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 39 |
| HpyF3I | CTNAG | 2 cut(s) | 14, 219 |
| Kzo9I | GATC | 2 cut(s) | 128, 272 |
| LpnPI | CCDG | 7 cut(s) | 19, 60, 68, 92, 117, 129, 156 |
| Lsp1109I | GCAGC | 2 cut(s) | 49, 193 |
| LweI | GCATC | 1 cut(s) | 51 |
| MaeI | CTAG | 1 cut(s) | 254 |
| MaeIII | GTNAC | 1 cut(s) | 116 |
| MalI | GATC | 2 cut(s) | 130, 274 |
| MboI | GATC | 2 cut(s) | 128, 272 |
| MflI | RGATCY | 2 cut(s) | 128, 272 |
| MlsI | TGGCCA | 1 cut(s) | 159 |
| MluCI | AATT | 1 cut(s) | 246 |
| MluNI | TGGCCA | 1 cut(s) | 159 |
| MlyI | GAGTC | 2 cut(s) | 59, 96 |
| MnlI | CCTC | 1 cut(s) | 128 |
| Mox20I | TGGCCA | 1 cut(s) | 159 |
| MscI | TGGCCA | 1 cut(s) | 159 |
| MseI | TTAA | 1 cut(s) | 125 |
| Msp20I | TGGCCA | 1 cut(s) | 159 |
| MspR9I | CCNGG | 1 cut(s) | 144 |
| Mva1269I | GAATGC | 1 cut(s) | 239 |
| MvaI | CCWGG | 1 cut(s) | 144 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 39 |
| NdeII | GATC | 2 cut(s) | 128, 272 |
| NmuCI | GTSAC | 1 cut(s) | 116 |
| PctI | GAATGC | 1 cut(s) | 239 |
| PfeI | GAWTC | 1 cut(s) | 139 |
| PfoI | TCCNGGA | 1 cut(s) | 142 |
| PkrI | GCNGC | 3 cut(s) | 64, 184, 208 |
| PleI | GAGTC | 2 cut(s) | 58, 95 |
| PpsI | GAGTC | 2 cut(s) | 58, 95 |
| Psp6I | CCWGG | 1 cut(s) | 142 |
| PspGI | CCWGG | 1 cut(s) | 142 |
| PsuI | RGATCY | 2 cut(s) | 128, 272 |
| SaqAI | TTAA | 1 cut(s) | 125 |
| SatI | GCNGC | 3 cut(s) | 63, 183, 207 |
| Sau3AI | GATC | 2 cut(s) | 128, 272 |
| SchI | GAGTC | 2 cut(s) | 59, 96 |
| ScrFI | CCNGG | 1 cut(s) | 144 |
| SetI | ASST | 3 cut(s) | 64, 111, 195 |
| SfaNI | GCATC | 1 cut(s) | 51 |
| Sse9I | AATT | 1 cut(s) | 246 |
| SsiI | CCGC | 3 cut(s) | 18, 98, 183 |
| SspMI | CTAG | 1 cut(s) | 254 |
| StyD4I | CCNGG | 1 cut(s) | 142 |
| TaqI | TCGA | 1 cut(s) | 53 |
| TasI | AATT | 1 cut(s) | 246 |
| TauI | GCSGC | 1 cut(s) | 185 |
| TfiI | GAWTC | 1 cut(s) | 139 |
| Tru1I | TTAA | 1 cut(s) | 125 |
| Tru9I | TTAA | 1 cut(s) | 125 |
| TseFI | GTSAC | 1 cut(s) | 116 |
| TseI | GCWGC | 2 cut(s) | 62, 206 |
| Tsp45I | GTSAC | 1 cut(s) | 116 |
| XspI | CTAG | 1 cut(s) | 254 |