Rroxscaffold_1G00018970

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001386
Physical Location & Seq
Reverse (-)
23068594 .. 23071655
3062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00018970.1

Sequence Viewer

Length: 1227 bp
ATGAAATTTCATTGTTTTCCCAGAGTTTCATATATTCTTGGAGAGCAGCAAAGTGACTCCCCTAAAAAAACAAAAGCAATGAGCCTCAGAATCCTGAGAAAGGCTTCACAAACCCAAAACCCAAAGCAACTCTCCTCCATTTTCTCCTCCAAAATCACACGCTCCCTCTCGCAGTTCTCCTCTCAGTCCAAACCTTCTTCTTCGTTTGGCATTGCTTTCGACATCGACGGCGTCGTTTCGCGTAGCGATGTTCCCATCGGAGGCTCCCCTCAGGCCTTCAGAAGACTATACGACGATTCTGGTCATCTCAGGATTCCGTACGTGTTCCTGACCAATGGAGGTGGGTTTCCTGAATCCAAAAGAGCACGTGAGTTAAGTGAGCTTTTGGGTGTCAAGGTTTCACCTTCACAGGTTTTGCAGGCTCATACACCTTTTAAGCAATTAGTGAGCAAATTTGAGAATGAGCTTATTGTTGCTGTGGGAAAAGGAGAACCTGCTGCTGTGATGTCCCATTACGGGTTTAGAAATGTGGTTTCAATTGATGACTATGCTGCGTGCTTTGAAAACATTGATCCATTAGCACCACATAAGAAGTGGACAACGAAGCAGGTTGGTAATCCAAATAGCGCTATCAGAGACCTTGTTCGCACAGAAAGAGTTCAAGCGGCTTTTGTACTTAGTGATTCTGTCGATTGGAGCAGGGACATTCAGGTTCTGTGTGACATTTTAAGAACCGGTGGTCTTCCTGGTCGGGAAATTGGACATCAACCACAACTGTACTTTGCGCATGATGACCTTGCATACCAGGCTGCTTTTCCTTCTGAGCGTTTTGGACTGGGAGCTTTCAGAATTGCATTGGAATCCATCTTTAACAGAATCCACCCTCATGCTCTCGAGTATACAACTTTTGGGAAGCCAAATTCATTTGTATTCAAGAATGCAGAACTTGTTCTTAAGCAACTTGTGTCGTCTGTTCATGTCAATGCTCAAGTTGCAAATCACGAAATTGCTGGCAGTCATCAGTTCAAAACGGTGTATATGGTAGGAGATAATCCCTTAGTTGACATCAAGGGTGCACAACAGGCTGGCCATCCCTGGTTTTCTATTCTGACAAGAACTGGAGTTTTCAAGGGAAAGGAAAATCATGACGAGTTTCCAGCTGATCTGGTTGTTGATACTGTCGAAGAAGCTGTGGACTATGTTTTGAAAAAGGAGTCGATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

408

Amino Acids

45.28

Weight (kDa)

8.77

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 71 - 175 4.3e-18 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 303 - 397 1.3e-11 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 786
Acc36I ACCTGC 2 cut(s) 502, 598
AccI GTMKAC 1 cut(s) 899
AccII CGCG 1 cut(s) 241
AciI CCGC 1 cut(s) 665
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 1087
AcsI RAATTY 3 cut(s) 5, 452, 919
AcuI CTGAAG 1 cut(s) 262
AcvI CACGTG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 3 cut(s) 320, 675, 779
AfeI AGCGCT 1 cut(s) 628
AfiI CCNNNNNNNGG 3 cut(s) 100, 260, 516
AflII CTTAAG 1 cut(s) 953
AflIII ACRYGT 1 cut(s) 321
AgeI ACCGGT 1 cut(s) 734
AgsI TTSAA 7 cut(s) 537, 563, 662, 934, 1027, 1129, 1207
AjnI CCWGG 3 cut(s) 745, 804, 1094
AluBI AGCT 5 cut(s) 382, 466, 842, 1160, 1190
AluI AGCT 5 cut(s) 382, 466, 842, 1160, 1190
Alw21I GWGCWC 2 cut(s) 367, 1078
Alw26I GTCTC 1 cut(s) 630
Alw44I GTGCAC 1 cut(s) 1074
AlwI GGATC 1 cut(s) 566
AlwNI CAGNNNCTG 1 cut(s) 715
Ama87I CYCGRG 1 cut(s) 893
Aor51HI AGCGCT 1 cut(s) 628
AoxI GGCC 2 cut(s) 273, 1087
ApaLI GTGCAC 1 cut(s) 1074
ApeKI GCWGC 4 cut(s) 46, 497, 551, 809
ApoI RAATTY 3 cut(s) 5, 452, 919
AsiGI ACCGGT 1 cut(s) 734
Asp700I GAANNNNTTC 3 cut(s) 103, 657, 948
AspLEI GCGC 2 cut(s) 629, 787
AsuHPI GGTGA 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 893
AxyI CCTNAGG 1 cut(s) 270
BaeGI GKGCMC 1 cut(s) 1078
BalI TGGCCA 1 cut(s) 1089
BbrPI CACGTG 1 cut(s) 368
BbsI GAAGAC 2 cut(s) 289, 734
Bbv12I GWGCWC 2 cut(s) 367, 1078
BbvI GCAGC 4 cut(s) 58, 484, 538, 796
BccI CCATC 3 cut(s) 263, 872, 1098
BceAI ACGGC 1 cut(s) 244
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BciT130I CCWGG 3 cut(s) 747, 806, 1096
BcoDI GTCTC 1 cut(s) 630
BfoI RGCGCY 1 cut(s) 630
BfrI CTTAAG 1 cut(s) 953
BfuAI ACCTGC 2 cut(s) 502, 598
BisI GCNGC 5 cut(s) 47, 498, 552, 666, 810
BlsI GCNGC 5 cut(s) 48, 499, 553, 667, 811
Bme1390I CCNGG 3 cut(s) 747, 806, 1096
BmeT110I CYCGRG 1 cut(s) 893
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 3 cut(s) 747, 806, 1096
BmrI ACTGGG 1 cut(s) 845
BmuI ACTGGG 1 cut(s) 845
BpiI GAAGAC 2 cut(s) 289, 734
BplI GAGNNNNNCTC 1 cut(s) 1205
BpmI CTGGAG 1 cut(s) 1140
BpuEI CTTGAG 1 cut(s) 972
BsaAI YACGTR 2 cut(s) 322, 368
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 630
BsaJI CCNNGG 1 cut(s) 1094
BsaWI WCCGGW 1 cut(s) 734
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 260, 516
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 2 cut(s) 840, 1123
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 2 cut(s) 84, 210
BseBI CCWGG 3 cut(s) 747, 806, 1096
BseDI CCNNGG 1 cut(s) 1094
BseGI GGATG 1 cut(s) 1090
BseLI CCNNNNNNNGG 3 cut(s) 100, 260, 516
BseMI GCAATG 2 cut(s) 84, 210
BseMII CTCAG 6 cut(s) 86, 100, 197, 284, 322, 813
BseNI ACTGG 2 cut(s) 840, 1123
BseRI GAGGAG 3 cut(s) 124, 136, 169
BseSI GKGCMC 1 cut(s) 1078
BseXI GCAGC 4 cut(s) 58, 484, 538, 796
Bsh1236I CGCG 1 cut(s) 241
BshFI GGCC 2 cut(s) 275, 1089
BshTI ACCGGT 1 cut(s) 734
BsiHKAI GWGCWC 2 cut(s) 367, 1078
BsiHKCI CYCGRG 1 cut(s) 893
BsiSI CCGG 1 cut(s) 735
BsiWI CGTACG 1 cut(s) 318
BslFI GGGAC 2 cut(s) 493, 716
BslI CCNNNNNNNGG 3 cut(s) 100, 260, 516
BsmAI GTCTC 1 cut(s) 630
BsmFI GGGAC 2 cut(s) 493, 716
BsmI GAATGC 1 cut(s) 943
BsnI GGCC 2 cut(s) 275, 1089
Bso31I GGTCTC 1 cut(s) 630
BsoBI CYCGRG 1 cut(s) 893
Bsp1286I GDGCHC 2 cut(s) 367, 1078
Bsp143I GATC 3 cut(s) 571, 1162, 1218
BspACI CCGC 1 cut(s) 665
BspANI GGCC 2 cut(s) 275, 1089
BspCNI CTCAG 6 cut(s) 87, 99, 196, 283, 321, 814
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 1 cut(s) 265
BspMI ACCTGC 2 cut(s) 502, 598
BspPI GGATC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 953
BspTNI GGTCTC 1 cut(s) 630
BsrDI GCAATG 2 cut(s) 84, 210
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 2 cut(s) 840, 1123
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 1 cut(s) 1094
BssMI GATC 3 cut(s) 571, 1162, 1218
BssNAI GTATAC 1 cut(s) 900
BssNI GRCGYC 1 cut(s) 231
Bst1107I GTATAC 1 cut(s) 900
Bst2UI CCWGG 3 cut(s) 747, 806, 1096
Bst4CI ACNGT 3 cut(s) 777, 1033, 1180
BstACI GRCGYC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 953
BstBAI YACGTR 2 cut(s) 322, 368
BstC8I GCNNGC 4 cut(s) 420, 556, 1012, 1087
BstDEI CTNAG 9 cut(s) 86, 95, 183, 270, 308, 677, 822, 1057, 1224
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 1 cut(s) 1090
BstFNI CGCG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 2 cut(s) 629, 787
BstKTI GATC 3 cut(s) 574, 1165, 1221
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 3 cut(s) 571, 1162, 1218
BstMWI GCNNNNNNNGC 3 cut(s) 806, 992, 1082
BstNI CCWGG 3 cut(s) 747, 806, 1096
BstSCI CCNGG 3 cut(s) 745, 804, 1094
BstSLI GKGCMC 1 cut(s) 1078
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 4 cut(s) 58, 484, 538, 796
BstV2I GAAGAC 2 cut(s) 289, 734
BstZ17I GTATAC 1 cut(s) 900
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 2 cut(s) 275, 1089
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 1 cut(s) 1090
BveI ACCTGC 2 cut(s) 502, 598
Cac8I GCNNGC 4 cut(s) 420, 556, 1012, 1087
CaiI CAGNNNCTG 1 cut(s) 715
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 2 cut(s) 629, 787
Cfr10I RCCGGY 1 cut(s) 734
CseI GACGC 1 cut(s) 220
Csp6I GTAC 3 cut(s) 319, 674, 778
CspAI ACCGGT 1 cut(s) 734
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 4 cut(s) 788, 887, 977, 1145
CviQI GTAC 3 cut(s) 319, 674, 778
DdeI CTNAG 9 cut(s) 86, 95, 183, 270, 308, 677, 822, 1057, 1224
DpnI GATC 3 cut(s) 573, 1164, 1220
DpnII GATC 3 cut(s) 571, 1162, 1218
EaeI YGGCCR 1 cut(s) 1087
Eco147I AGGCCT 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 630
Eco47III AGCGCT 1 cut(s) 628
Eco57I CTGAAG 1 cut(s) 262
Eco72I CACGTG 1 cut(s) 368
Eco81I CCTNAGG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 893
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoRII CCWGG 3 cut(s) 745, 804, 1094
FaeI CATG 4 cut(s) 791, 890, 980, 1148
FaqI GGGAC 2 cut(s) 493, 716
FatI CATG 4 cut(s) 787, 886, 976, 1144
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 5 cut(s) 47, 498, 552, 666, 810
FokI GGATG 1 cut(s) 1077
Fsp4HI GCNGC 5 cut(s) 47, 498, 552, 666, 810
FspI TGCGCA 1 cut(s) 786
GlaI GCGC 2 cut(s) 628, 786
GluI GCNGC 5 cut(s) 47, 498, 552, 666, 810
GsuI CTGGAG 1 cut(s) 1140
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 2 cut(s) 275, 1089
HapII CCGG 1 cut(s) 735
HgaI GACGC 1 cut(s) 220
HhaI GCGC 2 cut(s) 629, 787
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 4 cut(s) 791, 890, 980, 1148
Hin6I GCGC 2 cut(s) 627, 785
HinP1I GCGC 2 cut(s) 627, 785
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HinfI GANTC 9 cut(s) 56, 90, 296, 313, 353, 683, 860, 876, 1214
HpaII CCGG 1 cut(s) 735
HphI GGTGA 1 cut(s) 393
Hpy166II GTNNAC 5 cut(s) 597, 900, 1063, 1076, 1195
Hpy188I TCNGA 7 cut(s) 89, 260, 281, 635, 823, 848, 1110
Hpy188III TCNNGA 9 cut(s) 94, 310, 328, 350, 752, 893, 934, 1001, 1145
Hpy8I GTNNAC 5 cut(s) 597, 900, 1063, 1076, 1195
Hpy99I CGWCG 3 cut(s) 230, 236, 296
HpyAV CCTTC 4 cut(s) 204, 286, 414, 828
HpyCH4III ACNGT 3 cut(s) 777, 1033, 1180
HpyCH4IV ACGT 2 cut(s) 321, 367
HpyCH4V TGCA 6 cut(s) 418, 800, 854, 941, 995, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 806, 992, 1082
HpyF3I CTNAG 9 cut(s) 86, 95, 183, 270, 308, 677, 822, 1057, 1224
HpySE526I ACGT 2 cut(s) 321, 367
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 4 cut(s) 791, 890, 980, 1148
HspAI GCGC 2 cut(s) 627, 785
Kzo9I GATC 3 cut(s) 571, 1162, 1218
LmnI GCTCC 4 cut(s) 167, 269, 696, 839
Lsp1109I GCAGC 4 cut(s) 58, 484, 538, 796
MaeII ACGT 2 cut(s) 321, 367
MaeIII GTNAC 2 cut(s) 53, 719
MalI GATC 3 cut(s) 573, 1164, 1220
MboI GATC 3 cut(s) 571, 1162, 1218
MboII GAAGA 5 cut(s) 189, 192, 294, 734, 1196
MfeI CAATTG 1 cut(s) 537
MhlI GDGCHC 2 cut(s) 367, 1078
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 8 cut(s) 5, 440, 452, 537, 756, 849, 919, 1005
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 2 cut(s) 50, 1223
MnlI CCTC 9 cut(s) 95, 145, 157, 176, 190, 254, 279, 332, 894
Mox20I TGGCCA 1 cut(s) 1089
MroXI GAANNNNTTC 3 cut(s) 103, 657, 948
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 5 cut(s) 374, 435, 728, 870, 954
MslI CAYNNNNRTG 2 cut(s) 885, 981
Msp20I TGGCCA 1 cut(s) 1089
MspA1I CMGCKG 1 cut(s) 1160
MspCI CTTAAG 1 cut(s) 953
MspI CCGG 1 cut(s) 735
MspR9I CCNGG 3 cut(s) 747, 806, 1096
MunI CAATTG 1 cut(s) 537
Mva1269I GAATGC 1 cut(s) 943
MvaI CCWGG 3 cut(s) 747, 806, 1096
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 3 cut(s) 806, 992, 1082
NdeII GATC 3 cut(s) 571, 1162, 1218
NlaIII CATG 4 cut(s) 791, 890, 980, 1148
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 2 cut(s) 53, 719
NsbI TGCGCA 1 cut(s) 786
PaeR7I CTCGAG 1 cut(s) 893
PagI TCATGA 1 cut(s) 1144
PceI AGGCCT 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 943
PdmI GAANNNNTTC 3 cut(s) 103, 657, 948
PfeI GAWTC 7 cut(s) 90, 296, 313, 353, 683, 860, 876
Pfl23II CGTACG 1 cut(s) 318
PflFI GACNNNGTC 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 734
PkrI GCNGC 5 cut(s) 48, 499, 553, 667, 811
PleI GAGTC 2 cut(s) 50, 1222
PmaCI CACGTG 1 cut(s) 368
PmlI CACGTG 1 cut(s) 368
PpsI GAGTC 2 cut(s) 50, 1222
Ppu21I YACGTR 2 cut(s) 322, 368
Psp6I CCWGG 3 cut(s) 745, 804, 1094
PspCI CACGTG 1 cut(s) 368
PspGI CCWGG 3 cut(s) 745, 804, 1094
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 715
PsyI GACNNNGTC 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 3 cut(s) 320, 675, 779
RsaNI GTAC 3 cut(s) 319, 674, 778
RseI CAYNNNNRTG 2 cut(s) 885, 981
SaqAI TTAA 5 cut(s) 374, 435, 728, 870, 954
SatI GCNGC 5 cut(s) 47, 498, 552, 666, 810
Sau3AI GATC 3 cut(s) 571, 1162, 1218
SchI GAGTC 2 cut(s) 50, 1223
ScrFI CCNGG 3 cut(s) 747, 806, 1096
SduI GDGCHC 2 cut(s) 367, 1078
Sfr274I CTCGAG 1 cut(s) 893
SlaI CTCGAG 1 cut(s) 893
SmiMI CAYNNNNRTG 2 cut(s) 885, 981
SmlI CTYRAG 3 cut(s) 893, 953, 987
SmoI CTYRAG 3 cut(s) 893, 953, 987
Sse9I AATT 8 cut(s) 5, 440, 452, 537, 756, 849, 919, 1005
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 1 cut(s) 665
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 745, 804, 1094
TaaI ACNGT 3 cut(s) 777, 1033, 1180
TaiI ACGT 2 cut(s) 324, 370
TaqI TCGA 6 cut(s) 219, 225, 690, 894, 1182, 1217
TasI AATT 8 cut(s) 5, 440, 452, 537, 756, 849, 919, 1005
TatI WGTACW 2 cut(s) 673, 777
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 7 cut(s) 90, 296, 313, 353, 683, 860, 876
Tru1I TTAA 5 cut(s) 374, 435, 728, 870, 954
Tru9I TTAA 5 cut(s) 374, 435, 728, 870, 954
TseFI GTSAC 2 cut(s) 53, 719
TseI GCWGC 4 cut(s) 46, 497, 551, 809
Tsp45I GTSAC 2 cut(s) 53, 719
TspDTI ATGAA 4 cut(s) 17, 18, 912, 965
TspGWI ACGGA 1 cut(s) 306
Tth111I GACNNNGTC 1 cut(s) 230
Vha464I CTTAAG 1 cut(s) 953
VneI GTGCAC 1 cut(s) 1074
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 3 cut(s) 5, 452, 919
XcmI CCANNNNNNNNNTGG 1 cut(s) 591
XhoI CTCGAG 1 cut(s) 893
XmiI GTMKAC 1 cut(s) 899
XmnI GAANNNNTTC 3 cut(s) 103, 657, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.