Prupe.2G220600_v2.0.a1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001386
Physical Location & Seq
Forward (+)
24899272 .. 24902987
3716 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G220600.1

Sequence Viewer

Length: 1251 bp
ATGAGACAGTTACCGTTAAACTGTAGCCACGAGCACGAAGCTCAGAGAGCAAAGCTGAACCAGAAGAGAGACAGAGAGAACGCGATGACGCCGATGAGGATGACATACCGTACGGTCTTCAAGGTTTATCAGAGCAAAAATAGAGCTCCATATTTGTTTCAATCGCGTTCATACTCTCAGTCGCAGTGTCAATCTAGGCCCGAACCCGAACGGTCTTCGTTTGGAATTGCGTTTGACATTGACGGGGTTATTCTCCGTGGCCGTGTTCCCGTTGGAGGGTCTCCGCGAGCTTTAAGGAAGTTGTACGGAACATCAGGTACTTTGAAGGCTCCTTTTCTGTTTTTGACCAATGGAGGTGGCATACCGGAATCAAGACGAGCCGCTGAATTAACTGAACTTTTGGGAGTTAACATCTTGCCTTCTCAGGTTGTACAAGGTCACACACCTTTTAAAAGTTTGTTGAGAAGATATGAGAATGAACTCATTATTGCTCTTGGAAAAGGGGAACCGGCTCTTGTAATGTCTGATTATGGTTTCAAAAAAGTTCTCTCATTAGATGAGTATGCATCCTACTTCAAGAATATTGATCCTGTATCTCAATATAAGCTGTGGAGAACTAAGCAGGCATTGGATTGCAGCCACCCCAAGGAGTCTGTGCCAAGATATGATGTTTATTCTGACAGGGTTAGTGCAGCTTTTGTTGTCAGCGACCCTGTAGATTGGGGCAGGGACATTCAGGTTCTCTGTGACATACTAAGATCTGGAGGGTTTCCTGGACAAGAGAACGGGCACCAACCACCTCTATATTTTGCCGCAGATGATCTTGAGTATCAGGCTGCATTTCCTTCTGAGCGACTCGGAATGGGTGCTTTCAGAATTGCACTTGAAAGCATCTTCAACACGATTCACCATGATGCTTTGGAATATGTCTCTTTTGGAAAGCCAAATCCGTTTGTGTTTAAGAACACTGAAGCTATATTGACACAGCTTCAACCATCTCATTGTCATAATGATACAACAAATACGGGAAACTCTCAGTCGCATGCTTTCAAAACCCTTTACATGATTGGTGACAACCCTTCAGTTGATGTCAAAGGTGCTCAACAGGCTGGACATCCTTGGTTTTCTATTTTGACGAGGACAGGTGTTTTTAAGGGGAAAGATAATCATGCAGAGTTTCCAGCAGATCTGGTTGTGGATACCGTAGAAGAGGCAGTGGACTACATTCTGAAGAGAGAAGGAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

417

Amino Acids

46.69

Weight (kDa)

8.15

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 789
AccII CGCG 3 cut(s) 83, 166, 286
AciI CCGC 3 cut(s) 284, 381, 813
AclWI GGATC 1 cut(s) 581
AcoI YGGCCR 1 cut(s) 259
AcuI CTGAAG 3 cut(s) 990, 1065, 1250
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 4 cut(s) 112, 305, 319, 432
AfiI CCNNNNNNNGG 3 cut(s) 275, 276, 646
AgsI TTSAA 9 cut(s) 121, 161, 325, 538, 577, 887, 898, 992, 1051
AjnI CCWGG 1 cut(s) 772
AluBI AGCT 8 cut(s) 41, 55, 146, 290, 607, 695, 974, 988
AluI AGCT 8 cut(s) 41, 55, 146, 290, 607, 695, 974, 988
Alw21I GWGCWC 3 cut(s) 36, 148, 1102
Alw26I GTCTC 3 cut(s) 63, 285, 934
AlwI GGATC 1 cut(s) 581
AoxI GGCC 2 cut(s) 197, 259
ApeKI GCWGC 3 cut(s) 636, 692, 836
AspS9I GGNCC 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 899, 1082
BaeGI GKGCMC 1 cut(s) 792
BanI GGYRCC 1 cut(s) 789
BanII GRGCYC 1 cut(s) 148
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 2 cut(s) 109, 207
Bbv12I GWGCWC 3 cut(s) 36, 148, 1102
BbvI GCAGC 3 cut(s) 648, 704, 823
BccI CCATC 1 cut(s) 1003
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 1 cut(s) 774
BciVI GTATCC 1 cut(s) 1192
BcoDI GTCTC 3 cut(s) 63, 285, 934
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 2 cut(s) 22, 714
BfuI GTATCC 1 cut(s) 1192
BglII AGATCT 2 cut(s) 758, 1186
BisI GCNGC 5 cut(s) 381, 637, 693, 813, 837
BlsI GCNGC 5 cut(s) 382, 638, 694, 814, 838
Bme1390I CCNGG 1 cut(s) 774
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 3 cut(s) 330, 507, 791
BmrFI CCNGG 1 cut(s) 774
BmsI GCATC 3 cut(s) 575, 900, 904
BpiI GAAGAC 2 cut(s) 109, 207
BpmI CTGGAG 1 cut(s) 783
BpuEI CTTGAG 1 cut(s) 845
BsaHI GRCGYC 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 256, 645, 1118
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 276, 646
Bse118I RCCGGY 1 cut(s) 508
BseBI CCWGG 1 cut(s) 774
BseDI CCNNGG 3 cut(s) 256, 645, 1118
BseGI GGATG 3 cut(s) 105, 566, 1114
BseLI CCNNNNNNNGG 3 cut(s) 275, 276, 646
BseMII CTCAG 5 cut(s) 56, 191, 437, 840, 1049
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 3 cut(s) 648, 704, 823
BsgI GTGCAG 1 cut(s) 711
Bsh1236I CGCG 3 cut(s) 83, 166, 286
BshFI GGCC 2 cut(s) 199, 261
BshNI GGYRCC 1 cut(s) 789
BsiHKAI GWGCWC 3 cut(s) 36, 148, 1102
BsiSI CCGG 2 cut(s) 365, 509
BsiWI CGTACG 1 cut(s) 110
BslFI GGGAC 1 cut(s) 743
BslI CCNNNNNNNGG 3 cut(s) 275, 276, 646
BsmAI GTCTC 3 cut(s) 63, 285, 934
BsmFI GGGAC 1 cut(s) 743
BsnI GGCC 2 cut(s) 199, 261
Bso31I GGTCTC 1 cut(s) 285
Bsp1286I GDGCHC 4 cut(s) 36, 148, 792, 1102
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 586, 758, 820, 1186
BspACI CCGC 3 cut(s) 284, 381, 813
BspANI GGCC 2 cut(s) 199, 261
BspCNI CTCAG 5 cut(s) 55, 190, 436, 841, 1048
BspFNI CGCG 3 cut(s) 83, 166, 286
BspLI GGNNCC 3 cut(s) 330, 507, 791
BspPI GGATC 1 cut(s) 581
BspT107I GGYRCC 1 cut(s) 789
BspTNI GGTCTC 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 508
BsrGI TGTACA 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 3 cut(s) 256, 645, 1118
BssMI GATC 4 cut(s) 586, 758, 820, 1186
BssNI GRCGYC 1 cut(s) 89
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 645, 1118
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 7 cut(s) 9, 15, 23, 110, 115, 213, 1204
Bst6I CTCTTC 3 cut(s) 59, 1203, 1226
BstACI GRCGYC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 3 cut(s) 288, 624, 1044
BstDEI CTNAG 8 cut(s) 42, 177, 423, 618, 755, 849, 1035, 1248
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 3 cut(s) 105, 566, 1114
BstFNI CGCG 3 cut(s) 83, 166, 286
BstKTI GATC 4 cut(s) 589, 761, 823, 1189
BstMAI GTCTC 3 cut(s) 63, 285, 934
BstMBI GATC 4 cut(s) 586, 758, 820, 1186
BstMWI GCNNNNNNNGC 2 cut(s) 47, 1106
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 1 cut(s) 1046
BstSCI CCNGG 1 cut(s) 772
BstSFI CTRYAG 2 cut(s) 22, 714
BstSLI GKGCMC 1 cut(s) 792
BstUI CGCG 3 cut(s) 83, 166, 286
BstV1I GCAGC 3 cut(s) 648, 704, 823
BstV2I GAAGAC 2 cut(s) 109, 207
BstX2I RGATCY 2 cut(s) 758, 1186
BstYI RGATCY 2 cut(s) 758, 1186
BsuI GTATCC 1 cut(s) 1192
BsuRI GGCC 2 cut(s) 199, 261
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 98
BtsCI GGATG 3 cut(s) 105, 566, 1114
BtsI GCAGTG 2 cut(s) 191, 1221
BtsIMutI CAGTG 3 cut(s) 191, 966, 1221
Cac8I GCNNGC 3 cut(s) 288, 624, 1044
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 198
CseI GACGC 1 cut(s) 97
Csp6I GTAC 4 cut(s) 111, 304, 318, 431
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviAII CATG 4 cut(s) 911, 1043, 1063, 1169
CviQI GTAC 4 cut(s) 111, 304, 318, 431
DdeI CTNAG 8 cut(s) 42, 177, 423, 618, 755, 849, 1035, 1248
DpnI GATC 4 cut(s) 588, 760, 822, 1188
DpnII GATC 4 cut(s) 586, 758, 820, 1186
DraI TTTAAA 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 3 cut(s) 59, 1203, 1226
EarI CTCTTC 3 cut(s) 59, 1203, 1226
Ecl136II GAGCTC 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 645, 1118
Eco24I GRGCYC 1 cut(s) 148
Eco31I GGTCTC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 146
Eco57I CTGAAG 3 cut(s) 990, 1065, 1250
EcoICRI GAGCTC 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 772
EcoT14I CCWWGG 2 cut(s) 645, 1118
EcoT22I ATGCAT 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 645, 1118
FaeI CATG 4 cut(s) 914, 1046, 1066, 1172
FaqI GGGAC 1 cut(s) 743
FatI CATG 4 cut(s) 910, 1042, 1062, 1168
Fnu4HI GCNGC 5 cut(s) 381, 637, 693, 813, 837
FokI GGATG 3 cut(s) 112, 553, 1101
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 5 cut(s) 381, 637, 693, 813, 837
FspBI CTAG 1 cut(s) 195
GluI GCNGC 5 cut(s) 381, 637, 693, 813, 837
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 2 cut(s) 199, 261
HapII CCGG 2 cut(s) 365, 509
HgaI GACGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 4 cut(s) 914, 1046, 1066, 1172
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 4 cut(s) 368, 650, 855, 904
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 2 cut(s) 365, 509
HphI GGTGA 2 cut(s) 899, 1082
Hpy166II GTNNAC 2 cut(s) 409, 1219
Hpy188I TCNGA 8 cut(s) 45, 132, 526, 679, 850, 860, 875, 1230
Hpy188III TCNNGA 4 cut(s) 372, 577, 762, 824
Hpy8I GTNNAC 2 cut(s) 409, 1219
HpyAV CCTTC 5 cut(s) 319, 429, 855, 1089, 1232
HpyCH4III ACNGT 7 cut(s) 9, 15, 23, 110, 115, 213, 1204
HpyCH4V TGCA 6 cut(s) 566, 636, 692, 839, 881, 1172
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 1106
HpyF3I CTNAG 8 cut(s) 42, 177, 423, 618, 755, 849, 1035, 1248
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 4 cut(s) 914, 1046, 1066, 1172
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 4 cut(s) 586, 758, 820, 1186
LmnI GCTCC 2 cut(s) 151, 334
Lsp1109I GCAGC 3 cut(s) 648, 704, 823
LweI GCATC 3 cut(s) 575, 900, 904
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 4 cut(s) 9, 437, 746, 1070
MalI GATC 4 cut(s) 588, 760, 822, 1188
MboI GATC 4 cut(s) 586, 758, 820, 1186
MboII GAAGA 7 cut(s) 76, 109, 207, 477, 886, 1220, 1243
MflI RGATCY 2 cut(s) 758, 1186
MhlI GDGCHC 4 cut(s) 36, 148, 792, 1102
MluCI AATT 3 cut(s) 225, 386, 876
MlyI GAGTC 2 cut(s) 659, 849
MmeI TCCRAC 1 cut(s) 253
MnlI CCTC 7 cut(s) 90, 269, 347, 758, 810, 1131, 1204
Mph1103I ATGCAT 1 cut(s) 568
MseI TTAA 7 cut(s) 17, 293, 389, 408, 450, 960, 1152
MslI CAYNNNNRTG 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 365, 509
MspR9I CCNGG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 3 cut(s) 83, 166, 286
MwoI GCNNNNNNNGC 2 cut(s) 47, 1106
NdeII GATC 4 cut(s) 586, 758, 820, 1186
NlaIII CATG 4 cut(s) 914, 1046, 1066, 1172
NlaIV GGNNCC 3 cut(s) 330, 507, 791
NmuCI GTSAC 3 cut(s) 437, 746, 1070
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 1 cut(s) 1046
PaeI GCATGC 1 cut(s) 1046
PfeI GAWTC 2 cut(s) 368, 904
Pfl23II CGTACG 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 772
PkrI GCNGC 5 cut(s) 382, 638, 694, 814, 838
PleI GAGTC 2 cut(s) 658, 849
PpsI GAGTC 2 cut(s) 658, 849
Psp124BI GAGCTC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 772
PspGI CCWGG 1 cut(s) 772
PspLI CGTACG 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 330, 507, 791
PspPI GGNCC 1 cut(s) 198
PsrI GAACNNNNNNTAC 2 cut(s) 301, 333
PsuI RGATCY 2 cut(s) 758, 1186
RsaI GTAC 4 cut(s) 112, 305, 319, 432
RsaNI GTAC 4 cut(s) 111, 304, 318, 431
RseI CAYNNNNRTG 1 cut(s) 912
SacI GAGCTC 1 cut(s) 148
SaqAI TTAA 7 cut(s) 17, 293, 389, 408, 450, 960, 1152
SatI GCNGC 5 cut(s) 381, 637, 693, 813, 837
Sau3AI GATC 4 cut(s) 586, 758, 820, 1186
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 2 cut(s) 659, 849
ScrFI CCNGG 1 cut(s) 774
SduI GDGCHC 4 cut(s) 36, 148, 792, 1102
SfaNI GCATC 3 cut(s) 575, 900, 904
SfcI CTRYAG 2 cut(s) 22, 714
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 1 cut(s) 824
SmoI CTYRAG 1 cut(s) 824
SphI GCATGC 1 cut(s) 1046
Sse9I AATT 3 cut(s) 225, 386, 876
SsiI CCGC 3 cut(s) 284, 381, 813
SspI AATATT 1 cut(s) 583
SspMI CTAG 1 cut(s) 195
SstI GAGCTC 1 cut(s) 148
StyD4I CCNGG 1 cut(s) 772
StyI CCWWGG 2 cut(s) 645, 1118
TaaI ACNGT 7 cut(s) 9, 15, 23, 110, 115, 213, 1204
TasI AATT 3 cut(s) 225, 386, 876
TatI WGTACW 1 cut(s) 430
TauI GCSGC 2 cut(s) 383, 815
TfiI GAWTC 2 cut(s) 368, 904
Tru1I TTAA 7 cut(s) 17, 293, 389, 408, 450, 960, 1152
Tru9I TTAA 7 cut(s) 17, 293, 389, 408, 450, 960, 1152
TscAI CASTG 3 cut(s) 191, 973, 1221
TseFI GTSAC 3 cut(s) 437, 746, 1070
TseI GCWGC 3 cut(s) 636, 692, 836
Tsp45I GTSAC 3 cut(s) 437, 746, 1070
TspDTI ATGAA 2 cut(s) 159, 492
TspGWI ACGGA 3 cut(s) 245, 321, 939
TspRI CASTG 3 cut(s) 191, 973, 1221
XceI RCATGY 1 cut(s) 1046
XspI CTAG 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.