RchiOBHm_Chr1g0364391

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001386
Physical Location & Seq
Forward (+)
55580621 .. 55585344
4724 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58908

Sequence Viewer

Length: 1164 bp
ATGTCGCCGATGAGATACCGGACGGCGTTTAGGCTTGCCCGGAGCAGAAACCGACTGCCGTTTCCGTTCCGGTCGCGTCCGCACTCTTCTGAGTCTCGGATTCAATGCAGGCCTGAACCACGGTCTTCGTTTGGAATTGCGTTTGACATTGACGGAGTTATTCTCGGCGGCCGTGCTCCGATCGGCGGGTCTCCTCGAGCTTTGAGGAAGCTGTACGGAGCTTCAGGTACTTTGAAGGTTCCATTTCTGTTTTTGACCAATGGGGGTGGCATCCCAGAGTCGAGACGAGCTGCTGAACTAAGTGAAGTTCTGGGGGTCAATATCCTGCCTTCTCAGGTTGTACAGGGTCATACACCTTTTAGAAACTTGTTGAGGAGATATGAGAATGAATTGATTGTTGCTGTTGGAAAAGGGGAACCAGCTCTTGTAATGTCAGAGTATGGTTTCAAAAAAGTTCTCTCATTAGATGAGTATGCATCCTACTTCAAGGATATTGACCCGGTAGCTCAATATAAGATGTGGAGGACTAAGCAGGCATTGGATTGCAGTAGCCACTTGAAGGAATTGGTGCCAAGATACGATGTATACTCTGACAGGGTTAGTGCTGCTTTTGTCGTTAGTGATCCTGTAGATTGGGGCAGGGACATTCAGGTTCTCTGTGACATTTTAAGATCTGGAGGGATTCCTGGATGGGAGAATGGCAATCAGCCACCCCTTTATTTTGCCGCAGATGACCTTGAATATCAGGCTGCATTTCCTTCTCAGAGACTTGGAATGGGTGCTTTCAGAATTGCACTAGAAAGCATCTTCAACAGAATTCACCGTAGTACTTTGGAATATGTATCTTTCGGGAAGCCCAATCCGTTTGTATTTAAGAACACTGAAGCTCTATTGGCAGAGCTTCAACCATCTCAATTTGATGATCATATTTCAGAAAGTGGATGCTCTCAGTCACATGATTTCAAAACCCTATATATGATCGGAGACAACCCTTTGGTTGATGTCAAAGGTGCTCAACAGGCCGGACATCCCTGGTTTTCTATTTTGACAAGGACAGGTGTTTTTAAGGGTAAAGATAATCATAAGGAGTTTCCAGCAGATATGGTTGTGGATACTGTAGAAGAGGCAGTGGACTTCATTCTGAAAAGAGAAGGAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

387

Amino Acids

43.33

Weight (kDa)

8.24

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 46 - 150 8.1e-18 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 283 - 376 5.5e-12 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 585
AccII CGCG 1 cut(s) 76
AciI CCGC 4 cut(s) 80, 168, 186, 726
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 816
AcuI CTGAAG 2 cut(s) 207, 903
AfaI GTAC 4 cut(s) 215, 229, 342, 829
AfiI CCNNNNNNNGG 2 cut(s) 185, 559
AgsI TTSAA 9 cut(s) 104, 235, 448, 487, 559, 740, 811, 905, 964
AjnI CCWGG 2 cut(s) 685, 1031
AluBI AGCT 8 cut(s) 200, 211, 221, 290, 422, 506, 887, 901
AluI AGCT 8 cut(s) 200, 211, 221, 290, 422, 506, 887, 901
Alw21I GWGCWC 2 cut(s) 178, 1015
Alw26I GTCTC 5 cut(s) 99, 195, 277, 760, 978
AlwI GGATC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 3 cut(s) 110, 169, 1020
ApeKI GCWGC 3 cut(s) 290, 605, 749
ApoI RAATTY 1 cut(s) 816
AsuC2I CCSGG 2 cut(s) 40, 500
AsuHPI GGTGA 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 195
BanI GGYRCC 1 cut(s) 568
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 178, 1015
BbvI GCAGC 3 cut(s) 277, 592, 736
BccI CCATC 2 cut(s) 684, 916
BceAI ACGGC 3 cut(s) 39, 43, 156
BciT130I CCWGG 2 cut(s) 687, 1033
BciVI GTATCC 1 cut(s) 1105
BclI TGATCA 1 cut(s) 922
BcnI CCSGG 2 cut(s) 40, 500
BcoDI GTCTC 5 cut(s) 99, 195, 277, 760, 978
BfaI CTAG 1 cut(s) 797
BfmI CTRYAG 2 cut(s) 627, 1116
BfuI GTATCC 1 cut(s) 1105
BglII AGATCT 1 cut(s) 671
BisI GCNGC 5 cut(s) 169, 291, 606, 726, 750
BlsI GCNGC 5 cut(s) 170, 292, 607, 727, 751
BmcAI AGTACT 1 cut(s) 829
Bme1390I CCNGG 4 cut(s) 40, 500, 687, 1033
BmeT110I CYCGRG 1 cut(s) 195
BmiI GGNNCC 3 cut(s) 240, 417, 570
BmrFI CCNGG 4 cut(s) 40, 500, 687, 1033
BmsI GCATC 4 cut(s) 279, 485, 813, 932
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpmI CTGGAG 1 cut(s) 696
BpuMI CCSGG 2 cut(s) 40, 500
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 119, 1031
BsaWI WCCGGW 2 cut(s) 18, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 559
BseBI CCWGG 2 cut(s) 687, 1033
BseDI CCNNGG 2 cut(s) 119, 1031
BseGI GGATG 5 cut(s) 270, 476, 695, 947, 1027
BseLI CCNNNNNNNGG 2 cut(s) 185, 559
BseMII CTCAG 4 cut(s) 81, 347, 776, 962
BseRI GAGGAG 2 cut(s) 183, 388
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 277, 592, 736
Bsh1236I CGCG 1 cut(s) 76
Bsh1285I CGRYCG 3 cut(s) 74, 172, 183
BshFI GGCC 3 cut(s) 112, 171, 1022
BshNI GGYRCC 1 cut(s) 568
BsiEI CGRYCG 3 cut(s) 74, 172, 183
BsiHKAI GWGCWC 2 cut(s) 178, 1015
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 5 cut(s) 19, 40, 70, 500, 1023
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 2 cut(s) 185, 559
BsmAI GTCTC 5 cut(s) 99, 195, 277, 760, 978
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 3 cut(s) 112, 171, 1022
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 178, 1015
Bsp1407I TGTACA 1 cut(s) 340
Bsp143I GATC 5 cut(s) 180, 622, 671, 922, 978
BspACI CCGC 4 cut(s) 80, 168, 186, 726
BspANI GGCC 3 cut(s) 112, 171, 1022
BspCNI CTCAG 4 cut(s) 82, 346, 775, 961
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 240, 417, 570
BspPI GGATC 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 195
BsrGI TGTACA 1 cut(s) 340
BssECI CCNNGG 2 cut(s) 119, 1031
BssMI GATC 5 cut(s) 180, 622, 671, 922, 978
BssNAI GTATAC 1 cut(s) 586
Bst1107I GTATAC 1 cut(s) 586
Bst2UI CCWGG 2 cut(s) 687, 1033
Bst4CI ACNGT 3 cut(s) 123, 824, 1117
Bst6I CTCTTC 2 cut(s) 91, 1116
BstAUI TGTACA 1 cut(s) 340
BstC8I GCNNGC 3 cut(s) 36, 110, 534
BstDEI CTNAG 7 cut(s) 90, 299, 333, 528, 762, 948, 1161
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 5 cut(s) 270, 476, 695, 947, 1027
BstFNI CGCG 1 cut(s) 76
BstKTI GATC 5 cut(s) 183, 625, 674, 925, 981
BstMAI GTCTC 5 cut(s) 99, 195, 277, 760, 978
BstMBI GATC 5 cut(s) 180, 622, 671, 922, 978
BstMCI CGRYCG 3 cut(s) 74, 172, 183
BstMWI GCNNNNNNNGC 2 cut(s) 893, 1019
BstNI CCWGG 2 cut(s) 687, 1033
BstSCI CCNGG 4 cut(s) 38, 498, 685, 1031
BstSFI CTRYAG 2 cut(s) 627, 1116
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 3 cut(s) 277, 592, 736
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 671
BstYI RGATCY 1 cut(s) 671
BstZ17I GTATAC 1 cut(s) 586
BstZI CGGCCG 1 cut(s) 169
BsuI GTATCC 1 cut(s) 1105
BsuRI GGCC 3 cut(s) 112, 171, 1022
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 5 cut(s) 270, 476, 695, 947, 1027
BtsI GCAGTG 1 cut(s) 1134
BtsIMutI CAGTG 2 cut(s) 879, 1134
Cac8I GCNNGC 3 cut(s) 36, 110, 534
CseI GACGC 1 cut(s) 65
Csp6I GTAC 4 cut(s) 214, 228, 341, 828
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviAII CATG 1 cut(s) 956
CviQI GTAC 4 cut(s) 214, 228, 341, 828
DdeI CTNAG 7 cut(s) 90, 299, 333, 528, 762, 948, 1161
DpnI GATC 5 cut(s) 182, 624, 673, 924, 980
DpnII GATC 5 cut(s) 180, 622, 671, 922, 978
EaeI YGGCCR 1 cut(s) 169
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 2 cut(s) 91, 1116
EarI CTCTTC 2 cut(s) 91, 1116
EclXI CGGCCG 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 195
Eco52I CGGCCG 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 207, 903
Eco88I CYCGRG 1 cut(s) 195
EcoRI GAATTC 1 cut(s) 816
EcoRII CCWGG 2 cut(s) 685, 1031
EcoT22I ATGCAT 1 cut(s) 478
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 1 cut(s) 959
FaqI GGGAC 1 cut(s) 656
FatI CATG 1 cut(s) 955
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 1 cut(s) 922
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 5 cut(s) 169, 291, 606, 726, 750
FokI GGATG 5 cut(s) 257, 463, 702, 954, 1014
Fsp4HI GCNGC 5 cut(s) 169, 291, 606, 726, 750
FspBI CTAG 1 cut(s) 797
GluI GCNGC 5 cut(s) 169, 291, 606, 726, 750
GsuI CTGGAG 1 cut(s) 696
HaeIII GGCC 3 cut(s) 112, 171, 1022
HapII CCGG 5 cut(s) 19, 40, 70, 500, 1023
HgaI GACGC 1 cut(s) 65
Hin1II CATG 1 cut(s) 959
HinfI GANTC 4 cut(s) 92, 100, 278, 682
HpaII CCGG 5 cut(s) 19, 40, 70, 500, 1023
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 2 cut(s) 586, 1132
Hpy188III TCNNGA 3 cut(s) 282, 675, 850
Hpy8I GTNNAC 2 cut(s) 586, 1132
HpyAV CCTTC 5 cut(s) 229, 339, 553, 768, 1145
HpyCH4III ACNGT 3 cut(s) 123, 824, 1117
HpyCH4V TGCA 5 cut(s) 108, 476, 546, 752, 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 893, 1019
HpyF3I CTNAG 7 cut(s) 90, 299, 333, 528, 762, 948, 1161
Hsp92II CATG 1 cut(s) 959
Ksp22I TGATCA 1 cut(s) 922
Kzo9I GATC 5 cut(s) 180, 622, 671, 922, 978
LmnI GCTCC 3 cut(s) 42, 181, 218
Lsp1109I GCAGC 3 cut(s) 277, 592, 736
LweI GCATC 4 cut(s) 279, 485, 813, 932
MaeI CTAG 1 cut(s) 797
MaeIII GTNAC 2 cut(s) 659, 951
MalI GATC 5 cut(s) 182, 624, 673, 924, 980
MboI GATC 5 cut(s) 180, 622, 671, 922, 978
MboII GAAGA 4 cut(s) 78, 117, 799, 1133
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 2 cut(s) 178, 1015
MluCI AATT 6 cut(s) 135, 389, 563, 789, 816, 914
MlyI GAGTC 2 cut(s) 101, 287
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 6 cut(s) 198, 204, 366, 516, 671, 1117
Mph1103I ATGCAT 1 cut(s) 478
MseI TTAA 3 cut(s) 668, 873, 1065
MspI CCGG 5 cut(s) 19, 40, 70, 500, 1023
MspR9I CCNGG 4 cut(s) 40, 500, 687, 1033
MvaI CCWGG 2 cut(s) 687, 1033
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 893, 1019
NciI CCSGG 2 cut(s) 40, 500
NdeII GATC 5 cut(s) 180, 622, 671, 922, 978
NlaIII CATG 1 cut(s) 959
NlaIV GGNNCC 3 cut(s) 240, 417, 570
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 659, 951
NsiI ATGCAT 1 cut(s) 478
PaeR7I CTCGAG 1 cut(s) 195
PceI AGGCCT 1 cut(s) 112
PfeI GAWTC 2 cut(s) 100, 682
PfoI TCCNGGA 1 cut(s) 685
PkrI GCNGC 5 cut(s) 170, 292, 607, 727, 751
Ple19I CGATCG 1 cut(s) 183
PleI GAGTC 2 cut(s) 100, 286
PpsI GAGTC 2 cut(s) 100, 286
Psp6I CCWGG 2 cut(s) 685, 1031
PspGI CCWGG 2 cut(s) 685, 1031
PspN4I GGNNCC 3 cut(s) 240, 417, 570
PspXI VCTCGAGB 1 cut(s) 195
PsuI RGATCY 1 cut(s) 671
PvuI CGATCG 1 cut(s) 183
RsaI GTAC 4 cut(s) 215, 229, 342, 829
RsaNI GTAC 4 cut(s) 214, 228, 341, 828
SaqAI TTAA 3 cut(s) 668, 873, 1065
SatI GCNGC 5 cut(s) 169, 291, 606, 726, 750
Sau3AI GATC 5 cut(s) 180, 622, 671, 922, 978
ScaI AGTACT 1 cut(s) 829
SchI GAGTC 2 cut(s) 101, 287
ScrFI CCNGG 4 cut(s) 40, 500, 687, 1033
SduI GDGCHC 2 cut(s) 178, 1015
SfaNI GCATC 4 cut(s) 279, 485, 813, 932
SfcI CTRYAG 2 cut(s) 627, 1116
Sfr274I CTCGAG 1 cut(s) 195
SlaI CTCGAG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 6 cut(s) 135, 389, 563, 789, 816, 914
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 4 cut(s) 80, 168, 186, 726
SspMI CTAG 1 cut(s) 797
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 4 cut(s) 38, 498, 685, 1031
TaaI ACNGT 3 cut(s) 123, 824, 1117
TaqI TCGA 2 cut(s) 196, 281
TasI AATT 6 cut(s) 135, 389, 563, 789, 816, 914
TatI WGTACW 2 cut(s) 340, 827
TauI GCSGC 2 cut(s) 171, 728
TfiI GAWTC 2 cut(s) 100, 682
Tru1I TTAA 3 cut(s) 668, 873, 1065
Tru9I TTAA 3 cut(s) 668, 873, 1065
TscAI CASTG 2 cut(s) 886, 1134
TseFI GTSAC 2 cut(s) 659, 951
TseI GCWGC 3 cut(s) 290, 605, 749
Tsp45I GTSAC 2 cut(s) 659, 951
TspDTI ATGAA 2 cut(s) 402, 1126
TspGWI ACGGA 4 cut(s) 54, 168, 231, 852
TspRI CASTG 2 cut(s) 886, 1134
XapI RAATTY 1 cut(s) 816
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 585
XspI CTAG 1 cut(s) 797
ZrmI AGTACT 1 cut(s) 829
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.