Rroxscaffold_4G00292330

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001386
Physical Location & Seq
Reverse (-)
12664814 .. 12669485
4672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00292330.1

Sequence Viewer

Length: 1188 bp
ATGTCGCCGATGAGATACCGGACGGCGTTTAGGCTTTCCCGGAGCAGAAACCGACTGCCGTTTCCGTTCCGGTCGCGTCCGCACTCCTCTGAGTCTCGGATTCAATGCAGGCCTGAACCACGGTCTTCGTTTGGAATTGCGTTTGACATTGACGGAGTTATTCTCGGCGGCCGTGCTCCGATCGGCGGGTCTCCTCGAGCTTTGAGGAAGCTGTACGGAGCTTCAGGTACTTTGAAGGTTCCATTTCTGTTTTTGACCAATGGTAAGTTGGCCAAGTGGTTTACCAGGGGTGGCATCCCAGAGTCGAGACGAGCTGCTGAACTAAGTGAAGTTCTGGGGGTCAATATCCTGCCTTCTCAGGTTGTACAGGGTCATACACCTTTTAGAAACTTGTTGAGGAGATATGAGAATGAATTGATTGTTGCTGTTGGAAAAGGGGAACCAGCTCTTGTAATGTCAGAGTATGGTTTCAAAAAAGTTCTCTCATTAGATGAGTATGCATCCTACTTCAAGGATATTGACCCGGTAGCTCAATATAAGATGTGGAGAACTAAGCAGGCATTGGATTGCAGTAGCCACTTGAAGGAATTGGTGCCAAGATACAATGTATACTCTGACAGGGTTAGTGCTGCTTTTGTCGTTAGTGATCCTGTAGATTGGGGCAGGGACATTCAGGTTCTCTGTGACATTTTAAGATCTGGAGGGATTCCTGGATGGGAGAATGGCAATCAGCCACCCCTTTATTTTGCCGCAGATGACCTTGAATATCAGGCTGCATTTCCTTCTCAGAGACTTGGAATGGGTGCTTTCAGAATTGCACTAGAAAGCATCTTCAACAGAATTCACCATGGTACTTTGGAATATGTATCTTTCGGGAAGCCCAATCCGTTTGTATTTAAGAACACTGAAGCTCTATTGGCAGAGTTTCAACCATCTCAATTTGATGATCATATTTCAGAAAGTGGATGCTCTCAGTCACATGATTTCAAAACCCTATATATGATTGGAGACAACCCTTTGGTTGATGTCAAAGGTGCTCAACAGGCCGGACATCCCTGGTTTTCTATTTTGACAAGAACAGGTGTTTTTAAGGGAAAAGATAATCATAAGGAGTTTCCAGCAGATATGGTTGTGGATACTGTAGAAGAGGCAGTGGACTTCATTCTGAAAAGAGAAGGAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

395

Amino Acids

44.36

Weight (kDa)

8.88

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 46 - 158 1.8e-13 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 291 - 384 5.9e-12 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 592
AccI GTMKAC 1 cut(s) 609
AccII CGCG 1 cut(s) 76
AciI CCGC 4 cut(s) 80, 168, 186, 750
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 2 cut(s) 169, 270
AcsI RAATTY 1 cut(s) 840
AcuI CTGAAG 2 cut(s) 207, 927
AfaI GTAC 4 cut(s) 215, 229, 366, 853
AfiI CCNNNNNNNGG 2 cut(s) 185, 583
AgsI TTSAA 9 cut(s) 104, 235, 472, 511, 583, 764, 835, 929, 988
AjnI CCWGG 3 cut(s) 284, 709, 1055
AluBI AGCT 7 cut(s) 200, 211, 221, 314, 446, 530, 911
AluI AGCT 7 cut(s) 200, 211, 221, 314, 446, 530, 911
Alw21I GWGCWC 2 cut(s) 178, 1039
Alw26I GTCTC 5 cut(s) 99, 195, 301, 784, 1002
AlwI GGATC 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 195
AoxI GGCC 4 cut(s) 110, 169, 270, 1044
ApeKI GCWGC 3 cut(s) 314, 629, 773
ApoI RAATTY 1 cut(s) 840
AsuC2I CCSGG 2 cut(s) 40, 524
AsuHPI GGTGA 1 cut(s) 836
AvaI CYCGRG 1 cut(s) 195
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 178, 1039
BbvI GCAGC 3 cut(s) 301, 616, 760
BccI CCATC 2 cut(s) 708, 940
BceAI ACGGC 3 cut(s) 39, 43, 156
BciT130I CCWGG 3 cut(s) 286, 711, 1057
BciVI GTATCC 1 cut(s) 1129
BclI TGATCA 1 cut(s) 946
BcnI CCSGG 2 cut(s) 40, 524
BcoDI GTCTC 5 cut(s) 99, 195, 301, 784, 1002
BfaI CTAG 1 cut(s) 821
BfmI CTRYAG 2 cut(s) 651, 1140
BfuI GTATCC 1 cut(s) 1129
BglII AGATCT 1 cut(s) 695
BisI GCNGC 5 cut(s) 169, 315, 630, 750, 774
BlsI GCNGC 5 cut(s) 170, 316, 631, 751, 775
Bme1390I CCNGG 5 cut(s) 40, 286, 524, 711, 1057
BmeT110I CYCGRG 1 cut(s) 195
BmiI GGNNCC 3 cut(s) 240, 441, 594
BmrFI CCNGG 5 cut(s) 40, 286, 524, 711, 1057
BmsI GCATC 4 cut(s) 303, 509, 837, 956
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpmI CTGGAG 1 cut(s) 720
BpuMI CCSGG 2 cut(s) 40, 524
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 119, 285, 847, 1055
BsaWI WCCGGW 2 cut(s) 18, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 583
BseBI CCWGG 3 cut(s) 286, 711, 1057
BseDI CCNNGG 4 cut(s) 119, 285, 847, 1055
BseGI GGATG 5 cut(s) 294, 500, 719, 971, 1051
BseLI CCNNNNNNNGG 2 cut(s) 185, 583
BseMII CTCAG 4 cut(s) 81, 371, 800, 986
BseRI GAGGAG 3 cut(s) 76, 183, 412
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 301, 616, 760
Bsh1236I CGCG 1 cut(s) 76
Bsh1285I CGRYCG 3 cut(s) 74, 172, 183
BshFI GGCC 4 cut(s) 112, 171, 272, 1046
BshNI GGYRCC 1 cut(s) 592
BsiEI CGRYCG 3 cut(s) 74, 172, 183
BsiHKAI GWGCWC 2 cut(s) 178, 1039
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 5 cut(s) 19, 40, 70, 524, 1047
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 2 cut(s) 185, 583
BsmAI GTCTC 5 cut(s) 99, 195, 301, 784, 1002
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 680
BsnI GGCC 4 cut(s) 112, 171, 272, 1046
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 178, 1039
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 4 cut(s) 180, 646, 695, 946
Bsp19I CCATGG 1 cut(s) 847
BspACI CCGC 4 cut(s) 80, 168, 186, 750
BspANI GGCC 4 cut(s) 112, 171, 272, 1046
BspCNI CTCAG 4 cut(s) 82, 370, 799, 985
BspFNI CGCG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 240, 441, 594
BspPI GGATC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 592
BspTNI GGTCTC 1 cut(s) 195
BsrGI TGTACA 1 cut(s) 364
BssECI CCNNGG 4 cut(s) 119, 285, 847, 1055
BssMI GATC 4 cut(s) 180, 646, 695, 946
BssNAI GTATAC 1 cut(s) 610
BssT1I CCWWGG 1 cut(s) 847
Bst1107I GTATAC 1 cut(s) 610
Bst2UI CCWGG 3 cut(s) 286, 711, 1057
Bst4CI ACNGT 2 cut(s) 123, 1141
Bst6I CTCTTC 1 cut(s) 1140
BstAUI TGTACA 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 110, 558
BstDEI CTNAG 7 cut(s) 90, 323, 357, 552, 786, 972, 1185
BstDSI CCRYGG 2 cut(s) 119, 847
BstF5I GGATG 5 cut(s) 294, 500, 719, 971, 1051
BstFNI CGCG 1 cut(s) 76
BstKTI GATC 4 cut(s) 183, 649, 698, 949
BstMAI GTCTC 5 cut(s) 99, 195, 301, 784, 1002
BstMBI GATC 4 cut(s) 180, 646, 695, 946
BstMCI CGRYCG 3 cut(s) 74, 172, 183
BstMWI GCNNNNNNNGC 2 cut(s) 917, 1043
BstNI CCWGG 3 cut(s) 286, 711, 1057
BstSCI CCNGG 5 cut(s) 38, 284, 522, 709, 1055
BstSFI CTRYAG 2 cut(s) 651, 1140
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 3 cut(s) 301, 616, 760
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BstZ17I GTATAC 1 cut(s) 610
BstZI CGGCCG 1 cut(s) 169
BsuI GTATCC 1 cut(s) 1129
BsuRI GGCC 4 cut(s) 112, 171, 272, 1046
BtgI CCRYGG 2 cut(s) 119, 847
BtsCI GGATG 5 cut(s) 294, 500, 719, 971, 1051
BtsI GCAGTG 1 cut(s) 1158
BtsIMutI CAGTG 2 cut(s) 903, 1158
Cac8I GCNNGC 2 cut(s) 110, 558
CseI GACGC 1 cut(s) 65
Csp6I GTAC 4 cut(s) 214, 228, 365, 852
CviAII CATG 2 cut(s) 848, 980
CviQI GTAC 4 cut(s) 214, 228, 365, 852
DdeI CTNAG 7 cut(s) 90, 323, 357, 552, 786, 972, 1185
DpnI GATC 4 cut(s) 182, 648, 697, 948
DpnII GATC 4 cut(s) 180, 646, 695, 946
EaeI YGGCCR 2 cut(s) 169, 270
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
EclXI CGGCCG 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 847
Eco147I AGGCCT 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 195
Eco52I CGGCCG 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 207, 927
Eco88I CYCGRG 1 cut(s) 195
EcoRI GAATTC 1 cut(s) 840
EcoRII CCWGG 3 cut(s) 284, 709, 1055
EcoT14I CCWWGG 1 cut(s) 847
EcoT22I ATGCAT 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 847
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 2 cut(s) 851, 983
FaqI GGGAC 1 cut(s) 680
FatI CATG 2 cut(s) 847, 979
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 1 cut(s) 946
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 5 cut(s) 169, 315, 630, 750, 774
FokI GGATG 5 cut(s) 281, 487, 726, 978, 1038
Fsp4HI GCNGC 5 cut(s) 169, 315, 630, 750, 774
FspBI CTAG 1 cut(s) 821
GluI GCNGC 5 cut(s) 169, 315, 630, 750, 774
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 4 cut(s) 112, 171, 272, 1046
HapII CCGG 5 cut(s) 19, 40, 70, 524, 1047
HgaI GACGC 1 cut(s) 65
Hin1II CATG 2 cut(s) 851, 983
HinfI GANTC 4 cut(s) 92, 100, 302, 706
HpaII CCGG 5 cut(s) 19, 40, 70, 524, 1047
HphI GGTGA 1 cut(s) 836
Hpy166II GTNNAC 3 cut(s) 282, 610, 1156
Hpy188I TCNGA 9 cut(s) 91, 99, 180, 460, 616, 789, 812, 958, 1167
Hpy188III TCNNGA 3 cut(s) 306, 699, 874
Hpy8I GTNNAC 3 cut(s) 282, 610, 1156
HpyAV CCTTC 5 cut(s) 229, 363, 577, 792, 1169
HpyCH4III ACNGT 2 cut(s) 123, 1141
HpyCH4V TGCA 5 cut(s) 108, 500, 570, 776, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 917, 1043
HpyF3I CTNAG 7 cut(s) 90, 323, 357, 552, 786, 972, 1185
Hsp92II CATG 2 cut(s) 851, 983
Ksp22I TGATCA 1 cut(s) 946
Kzo9I GATC 4 cut(s) 180, 646, 695, 946
LmnI GCTCC 3 cut(s) 42, 181, 218
Lsp1109I GCAGC 3 cut(s) 301, 616, 760
LweI GCATC 4 cut(s) 303, 509, 837, 956
MaeI CTAG 1 cut(s) 821
MaeIII GTNAC 2 cut(s) 683, 975
MalI GATC 4 cut(s) 182, 648, 697, 948
MboI GATC 4 cut(s) 180, 646, 695, 946
MboII GAAGA 3 cut(s) 117, 823, 1157
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 178, 1039
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 6 cut(s) 135, 413, 587, 813, 840, 938
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 2 cut(s) 101, 311
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 6 cut(s) 97, 198, 204, 390, 695, 1141
Mox20I TGGCCA 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 502
MscI TGGCCA 1 cut(s) 272
MseI TTAA 3 cut(s) 692, 897, 1089
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 5 cut(s) 19, 40, 70, 524, 1047
MspR9I CCNGG 5 cut(s) 40, 286, 524, 711, 1057
MvaI CCWGG 3 cut(s) 286, 711, 1057
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 917, 1043
NciI CCSGG 2 cut(s) 40, 524
NcoI CCATGG 1 cut(s) 847
NdeII GATC 4 cut(s) 180, 646, 695, 946
NlaIII CATG 2 cut(s) 851, 983
NlaIV GGNNCC 3 cut(s) 240, 441, 594
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 683, 975
NsiI ATGCAT 1 cut(s) 502
PaeR7I CTCGAG 1 cut(s) 195
PceI AGGCCT 1 cut(s) 112
PfeI GAWTC 2 cut(s) 100, 706
PfoI TCCNGGA 2 cut(s) 38, 709
PkrI GCNGC 5 cut(s) 170, 316, 631, 751, 775
Ple19I CGATCG 1 cut(s) 183
PleI GAGTC 2 cut(s) 100, 310
PpsI GAGTC 2 cut(s) 100, 310
Psp6I CCWGG 3 cut(s) 284, 709, 1055
PspGI CCWGG 3 cut(s) 284, 709, 1055
PspN4I GGNNCC 3 cut(s) 240, 441, 594
PspXI VCTCGAGB 1 cut(s) 195
PsuI RGATCY 1 cut(s) 695
PvuI CGATCG 1 cut(s) 183
RsaI GTAC 4 cut(s) 215, 229, 366, 853
RsaNI GTAC 4 cut(s) 214, 228, 365, 852
SaqAI TTAA 3 cut(s) 692, 897, 1089
SatI GCNGC 5 cut(s) 169, 315, 630, 750, 774
Sau3AI GATC 4 cut(s) 180, 646, 695, 946
SchI GAGTC 2 cut(s) 101, 311
ScrFI CCNGG 5 cut(s) 40, 286, 524, 711, 1057
SduI GDGCHC 2 cut(s) 178, 1039
SfaNI GCATC 4 cut(s) 303, 509, 837, 956
SfcI CTRYAG 2 cut(s) 651, 1140
Sfr274I CTCGAG 1 cut(s) 195
SlaI CTCGAG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 6 cut(s) 135, 413, 587, 813, 840, 938
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 4 cut(s) 80, 168, 186, 750
SspMI CTAG 1 cut(s) 821
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 5 cut(s) 38, 284, 522, 709, 1055
StyI CCWWGG 1 cut(s) 847
TaaI ACNGT 2 cut(s) 123, 1141
TaqI TCGA 2 cut(s) 196, 305
TasI AATT 6 cut(s) 135, 413, 587, 813, 840, 938
TatI WGTACW 1 cut(s) 364
TauI GCSGC 2 cut(s) 171, 752
TfiI GAWTC 2 cut(s) 100, 706
Tru1I TTAA 3 cut(s) 692, 897, 1089
Tru9I TTAA 3 cut(s) 692, 897, 1089
TscAI CASTG 2 cut(s) 910, 1158
TseFI GTSAC 2 cut(s) 683, 975
TseI GCWGC 3 cut(s) 314, 629, 773
Tsp45I GTSAC 2 cut(s) 683, 975
TspDTI ATGAA 2 cut(s) 426, 1150
TspGWI ACGGA 4 cut(s) 54, 168, 231, 876
TspRI CASTG 2 cut(s) 910, 1158
XapI RAATTY 1 cut(s) 840
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 1 cut(s) 821
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.