Prupe.6G090300_v2.0.a1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001386
Physical Location & Seq
Reverse (-)
6178637 .. 6182636
4000 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G090300.2

Sequence Viewer

Length: 1137 bp
ATGAGCCTCCGAATACTGAGAAAGGCTTCGCAGACCCAAAACGCAAAGCAATTATCCTCCAGTTTCTCCAAATTCACACGATCATTCTCGCAGCTCTCCTCACAGTCCAAACCGCCTTCCTTTGGCATTGCGTTCGACATCGACGGCGTCGTTTTACGCAGCGAAGCTCCGATCGGAGGCTCTCCTCAGGTCTTCAGAAGATTATACGACGATTCTGGTAATCTTAGCATTCCGTACGTGTTCTTGACAAATGGTGGTGGGTTTACTGAATCTAAAAGAGCCCTTGAGTTAAGTGAACTTTTGGGTGTCAAGATTTCACCTTTACAGGTTTTACAGGGCCATACACCTTTTAAGCAGTTAGTAAACAGATTTGAGAATGAACTCATTGTTGCTGTGGGGAAAGGAGAACCTGCTGCAGTTATGTCTGAATATGGATTTAAAAATGTTCTTTCAATTGATGACTATGCGTCCTGCTTTGAAAACATTGACCCCCTAGCACCGTATAAAAAATGGACAACTAAGCAGGTTAAGGAGGTTGCTGCCAGAGACCTTGTATTCTCACAAAGAGTTCAGGCGGCGTTTATAGTTAGTGATTCTGTTGATTGGAGCAGAGACATTCAGGTCCTATGCGACATTTTAAGAACTGGTGGTCTTCCTGGTCGGGAAATAGGACACCAACCACATCTTTATTTTGCGCATGATGACCTTGCATACCAGGCTGCTTTTCCTACCGAGCGTTTTGGAATGGGAGCTTTCAGAATTGCACTAGAATCCATCTTTAATAGAATCCATCCTCATGCTCTGGAGTATACAACTTTTGGGAAACCAAATCCATTTGTATTCAATAATGCAGAACTTGTACTTAAGCAACTTGTGTCAGCTCTTCATGATAATCTTCAAGTTGCCAATCAAGCAAATGCTGGAAGTTACCAATTCCAAACACTGTATATGATAGGAGATAATCCTTCGGTTGACATCAATGGTGCACGACAGGCGGGTCATCCCTGGTTTTCTATTCTGACAAGGACTGGGGTTTTCAAGGGGAAGGAAAATCATGCCGAATTTCCAGCGGATCTGGTTGTTGACACGGTGGAAGAAGCTGTAGACTTTGTTTTGAGAAAGGAATGCATCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

379

Amino Acids

41.97

Weight (kDa)

6.32

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 620, 996
Acc16I TGCGCA 1 cut(s) 696
Acc36I ACCTGC 2 cut(s) 418, 514
AccI GTMKAC 2 cut(s) 809, 1104
AciI CCGC 4 cut(s) 113, 575, 995, 1070
AclWI GGATC 1 cut(s) 1080
AcsI RAATTY 2 cut(s) 71, 1061
AcuI CTGAAG 1 cut(s) 178
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 2 cut(s) 236, 861
AfiI CCNNNNNNNGG 2 cut(s) 122, 176
AflII CTTAAG 1 cut(s) 863
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 5 cut(s) 453, 479, 844, 899, 1039
AhdI GACNNNNNGTC 1 cut(s) 466
AjnI CCWGG 3 cut(s) 655, 714, 1004
AluBI AGCT 5 cut(s) 94, 167, 752, 881, 1100
AluI AGCT 5 cut(s) 94, 167, 752, 881, 1100
Alw21I GWGCWC 1 cut(s) 988
Alw26I GTCTC 2 cut(s) 540, 606
Alw44I GTGCAC 1 cut(s) 984
AlwI GGATC 1 cut(s) 1080
AoxI GGCC 1 cut(s) 337
ApaLI GTGCAC 1 cut(s) 984
ApeKI GCWGC 5 cut(s) 91, 159, 413, 539, 719
ApoI RAATTY 2 cut(s) 71, 1061
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 1 cut(s) 697
AspS9I GGNCC 2 cut(s) 337, 622
AsuHPI GGTGA 1 cut(s) 309
AvaII GGWCC 1 cut(s) 622
AxyI CCTNAGG 1 cut(s) 186
BaeGI GKGCMC 1 cut(s) 988
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 2 cut(s) 184, 644
Bbv12I GWGCWC 1 cut(s) 988
BbvI GCAGC 5 cut(s) 103, 171, 400, 526, 706
BccI CCATC 2 cut(s) 782, 798
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 3 cut(s) 657, 716, 1006
BcoDI GTCTC 2 cut(s) 540, 606
BfaI CTAG 3 cut(s) 494, 767, 1135
BfmI CTRYAG 2 cut(s) 414, 1101
BfrI CTTAAG 1 cut(s) 863
BfuAI ACCTGC 2 cut(s) 418, 514
BisI GCNGC 6 cut(s) 92, 160, 414, 540, 576, 720
BlsI GCNGC 6 cut(s) 93, 161, 415, 541, 577, 721
Bme1390I CCNGG 3 cut(s) 657, 716, 1006
Bme18I GGWCC 1 cut(s) 622
BmeRI GACNNNNNGTC 1 cut(s) 466
BmgT120I GGNCC 2 cut(s) 337, 622
BmrFI CCNGG 3 cut(s) 657, 716, 1006
BmrI ACTGGG 1 cut(s) 1038
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 2 cut(s) 184, 644
BpmI CTGGAG 2 cut(s) 43, 824
BpuEI CTTGAG 1 cut(s) 305
BsaAI YACGTR 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 1 cut(s) 1004
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 176
Bse1I ACTGG 3 cut(s) 60, 649, 1033
Bse21I CCTNAGG 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 126
BseBI CCWGG 3 cut(s) 657, 716, 1006
BseDI CCNNGG 1 cut(s) 1004
BseGI GGATG 2 cut(s) 790, 1000
BseLI CCNNNNNNNGG 2 cut(s) 122, 176
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 2 cut(s) 8, 200
BseNI ACTGG 3 cut(s) 60, 649, 1033
BseRI GAGGAG 2 cut(s) 88, 174
BseSI GKGCMC 1 cut(s) 988
BseXI GCAGC 5 cut(s) 103, 171, 400, 526, 706
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 339
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 988
BsiWI CGTACG 1 cut(s) 234
BslI CCNNNNNNNGG 2 cut(s) 122, 176
BsmAI GTCTC 2 cut(s) 540, 606
BsmI GAATGC 2 cut(s) 228, 1130
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 540
Bsp1286I GDGCHC 2 cut(s) 283, 988
Bsp143I GATC 3 cut(s) 80, 171, 1072
BspACI CCGC 4 cut(s) 113, 575, 995, 1070
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 2 cut(s) 9, 199
BspHI TCATGA 1 cut(s) 886
BspMAI CTGCAG 1 cut(s) 418
BspMI ACCTGC 2 cut(s) 418, 514
BspPI GGATC 1 cut(s) 1080
BspQI GCTCTTC 1 cut(s) 888
BspTI CTTAAG 1 cut(s) 863
BspTNI GGTCTC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 126
BsrI ACTGG 3 cut(s) 60, 649, 1033
BssECI CCNNGG 1 cut(s) 1004
BssMI GATC 3 cut(s) 80, 171, 1072
BssNAI GTATAC 1 cut(s) 810
BssNI GRCGYC 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 810
Bst2UI CCWGG 3 cut(s) 657, 716, 1006
Bst4CI ACNGT 4 cut(s) 105, 501, 945, 1090
Bst6I CTCTTC 1 cut(s) 888
BstACI GRCGYC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 238
BstDEI CTNAG 4 cut(s) 17, 186, 224, 519
BstF5I GGATG 2 cut(s) 790, 1000
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 3 cut(s) 83, 174, 1075
BstMAI GTCTC 2 cut(s) 540, 606
BstMBI GATC 3 cut(s) 80, 171, 1072
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 3 cut(s) 716, 911, 992
BstNI CCWGG 3 cut(s) 657, 716, 1006
BstSCI CCNGG 3 cut(s) 655, 714, 1004
BstSFI CTRYAG 2 cut(s) 414, 1101
BstSLI GKGCMC 1 cut(s) 988
BstV1I GCAGC 5 cut(s) 103, 171, 400, 526, 706
BstV2I GAAGAC 2 cut(s) 184, 644
BstX2I RGATCY 1 cut(s) 1072
BstYI RGATCY 1 cut(s) 1072
BstZ17I GTATAC 1 cut(s) 810
Bsu36I CCTNAGG 1 cut(s) 186
BsuRI GGCC 1 cut(s) 339
BtsCI GGATG 2 cut(s) 790, 1000
BtsIMutI CAGTG 1 cut(s) 941
BveI ACCTGC 2 cut(s) 418, 514
CciI TCATGA 1 cut(s) 886
CfoI GCGC 1 cut(s) 697
Cfr13I GGNCC 2 cut(s) 337, 622
CseI GACGC 2 cut(s) 136, 456
Csp6I GTAC 2 cut(s) 235, 860
CviAII CATG 4 cut(s) 698, 797, 887, 1055
CviQI GTAC 2 cut(s) 235, 860
DdeI CTNAG 4 cut(s) 17, 186, 224, 519
DpnI GATC 3 cut(s) 82, 173, 1074
DpnII GATC 3 cut(s) 80, 171, 1072
DraI TTTAAA 1 cut(s) 439
DrdI GACNNNNNNGTC 2 cut(s) 620, 996
DriI GACNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 2 cut(s) 620, 996
Eam1104I CTCTTC 1 cut(s) 888
Eam1105I GACNNNNNGTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 888
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 540
Eco47I GGWCC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 178
Eco81I CCTNAGG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 622
EcoRII CCWGG 3 cut(s) 655, 714, 1004
EcoT22I ATGCAT 1 cut(s) 1130
EcoT38I GRGCYC 1 cut(s) 283
FaeI CATG 4 cut(s) 701, 800, 890, 1058
FatI CATG 4 cut(s) 697, 796, 886, 1054
FauI CCCGC 1 cut(s) 988
FblI GTMKAC 2 cut(s) 809, 1104
Fnu4HI GCNGC 6 cut(s) 92, 160, 414, 540, 576, 720
FokI GGATG 2 cut(s) 777, 987
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 6 cut(s) 92, 160, 414, 540, 576, 720
FspBI CTAG 3 cut(s) 494, 767, 1135
FspI TGCGCA 1 cut(s) 696
GlaI GCGC 1 cut(s) 696
GluI GCNGC 6 cut(s) 92, 160, 414, 540, 576, 720
GsuI CTGGAG 2 cut(s) 43, 824
HaeIII GGCC 1 cut(s) 339
HgaI GACGC 2 cut(s) 136, 456
HhaI GCGC 1 cut(s) 697
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 701, 800, 890, 1058
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HincII GTYRAC 2 cut(s) 973, 1084
HindII GTYRAC 2 cut(s) 973, 1084
HinfI GANTC 5 cut(s) 212, 269, 593, 770, 786
HphI GGTGA 1 cut(s) 309
Hpy166II GTNNAC 8 cut(s) 264, 296, 364, 810, 973, 986, 1084, 1105
Hpy188I TCNGA 7 cut(s) 11, 171, 176, 197, 427, 758, 1020
Hpy188III TCNNGA 5 cut(s) 244, 310, 662, 803, 887
Hpy8I GTNNAC 8 cut(s) 264, 296, 364, 810, 973, 986, 1084, 1105
Hpy99I CGWCG 3 cut(s) 146, 152, 212
HpyAV CCTTC 3 cut(s) 126, 975, 1039
HpyCH4III ACNGT 4 cut(s) 105, 501, 945, 1090
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 6 cut(s) 416, 710, 764, 851, 986, 1128
HpyF10VI GCNNNNNNNGC 3 cut(s) 716, 911, 992
HpyF3I CTNAG 4 cut(s) 17, 186, 224, 519
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 701, 800, 890, 1058
HspAI GCGC 1 cut(s) 695
Kzo9I GATC 3 cut(s) 80, 171, 1072
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 3 cut(s) 172, 606, 749
Lsp1109I GCAGC 5 cut(s) 103, 171, 400, 526, 706
MaeI CTAG 3 cut(s) 494, 767, 1135
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 926
MalI GATC 3 cut(s) 82, 173, 1074
MboI GATC 3 cut(s) 80, 171, 1072
MboII GAAGA 6 cut(s) 184, 210, 644, 875, 887, 1106
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 1 cut(s) 1072
MhlI GDGCHC 2 cut(s) 283, 988
MluCI AATT 6 cut(s) 50, 71, 453, 759, 932, 1061
MnlI CCTC 7 cut(s) 17, 67, 109, 170, 195, 526, 804
Mph1103I ATGCAT 1 cut(s) 1130
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 7 cut(s) 290, 351, 438, 528, 638, 780, 864
MslI CAYNNNNRTG 1 cut(s) 795
MspA1I CMGCKG 1 cut(s) 1070
MspCI CTTAAG 1 cut(s) 863
MspR9I CCNGG 3 cut(s) 657, 716, 1006
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 2 cut(s) 228, 1130
MvaI CCWGG 3 cut(s) 657, 716, 1006
MwoI GCNNNNNNNGC 3 cut(s) 716, 911, 992
NdeII GATC 3 cut(s) 80, 171, 1072
NlaIII CATG 4 cut(s) 701, 800, 890, 1058
NsbI TGCGCA 1 cut(s) 696
NsiI ATGCAT 1 cut(s) 1130
PagI TCATGA 1 cut(s) 886
PciSI GCTCTTC 1 cut(s) 888
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 228, 1130
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 5 cut(s) 212, 269, 593, 770, 786
Pfl23II CGTACG 1 cut(s) 234
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 6 cut(s) 93, 161, 415, 541, 577, 721
Ple19I CGATCG 1 cut(s) 174
Ppu21I YACGTR 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 622
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 3 cut(s) 655, 714, 1004
PspGI CCWGG 3 cut(s) 655, 714, 1004
PspLI CGTACG 1 cut(s) 234
PspPI GGNCC 2 cut(s) 337, 622
PspPPI RGGWCCY 1 cut(s) 622
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 1072
PsyI GACNNNGTC 1 cut(s) 146
PvuI CGATCG 1 cut(s) 174
RsaI GTAC 2 cut(s) 236, 861
RsaNI GTAC 2 cut(s) 235, 860
RseI CAYNNNNRTG 1 cut(s) 795
SapI GCTCTTC 1 cut(s) 888
SaqAI TTAA 7 cut(s) 290, 351, 438, 528, 638, 780, 864
SatI GCNGC 6 cut(s) 92, 160, 414, 540, 576, 720
Sau3AI GATC 3 cut(s) 80, 171, 1072
Sau96I GGNCC 2 cut(s) 337, 622
ScrFI CCNGG 3 cut(s) 657, 716, 1006
SduI GDGCHC 2 cut(s) 283, 988
SfcI CTRYAG 2 cut(s) 414, 1101
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 2 cut(s) 284, 863
SmoI CTYRAG 2 cut(s) 284, 863
Sse9I AATT 6 cut(s) 50, 71, 453, 759, 932, 1061
SsiI CCGC 4 cut(s) 113, 575, 995, 1070
SspMI CTAG 3 cut(s) 494, 767, 1135
StyD4I CCNGG 3 cut(s) 655, 714, 1004
TaaI ACNGT 4 cut(s) 105, 501, 945, 1090
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 135, 141
TasI AATT 6 cut(s) 50, 71, 453, 759, 932, 1061
TatI WGTACW 1 cut(s) 859
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 5 cut(s) 212, 269, 593, 770, 786
Tru1I TTAA 7 cut(s) 290, 351, 438, 528, 638, 780, 864
Tru9I TTAA 7 cut(s) 290, 351, 438, 528, 638, 780, 864
TscAI CASTG 1 cut(s) 948
TseI GCWGC 5 cut(s) 91, 159, 413, 539, 719
TspDTI ATGAA 2 cut(s) 393, 875
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 1 cut(s) 948
Tth111I GACNNNGTC 1 cut(s) 146
Vha464I CTTAAG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 984
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 2 cut(s) 71, 1061
XmiI GTMKAC 2 cut(s) 809, 1104
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 3 cut(s) 494, 767, 1135
Zsp2I ATGCAT 1 cut(s) 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.