Rw5G037990

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001386
Physical Location & Seq
Forward (+)
67208237 .. 67210983
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037990.1

Sequence Viewer

Length: 1149 bp
ATGAGCCTCAGAATCGTGAGAAAGGCTTCACAAACCCAAAACCCGAAGCAACTCTCCTCCATTTTCTTCTCCAAAATCACACGCTCCCTCTCGCAGCTCTCCTCTCAATCCAAACCTTCTTCTTCGTTTGCCATTGCTTTCGACATCGACGGCGTCGTTTCCCGTAGCGATGTTCCCATCGGAGGCTCCCCTCAGGCCTTCAGAAGACTATACGACGATTCTGGTCATCCCAGGATTCCGTACGTGTTCCTGACCAATGGAGGTGGGTTTCCCGAGTCCAAAAGAGCACGTGAGTTAAGTGAGCTTTTGGGTGTCAAGGTTTCACCTTCACAGGTTTTGCAGGCTCATACACCTTTTAAGCAATTAGTGAGCAAATTTGAGAATGAGCTTATTGTTGCTGTGGGAAAAGGAGAACCTGCTGCTGTCATGTCCCATTACGGGTTTAGAAATGTGGTTTCAATTGATGACTATGCCGCGTGCTTTGAAAACATTGATCCATTAGCACCACATAAGAAGTGGACAACGAAGCAGGTTGGTAATCCGAATAGCGCTATCAGAGACCTTGTTCGCACAGAAAGAGTTCAGGCGGCGTTTGTACTTAGTGATTCTGTCGATTGGAGCAGGGACATTCAGGTTCTGTGTGACATTTTAAGAACCGGTGGTCTTCCTGGTCGGGAAATTGGACATCAACCACAACTGTACTTTGCGCATGATGACCTTGCATACCAGGCTGCTTTTCCTTCTGAGCGTTTTGGACTGGGAGCTTTCAGAATTGCATTGGAATCCATCTTTAACAGAATCCACCCTCATGCTCTGGAGTATACAACTTTTGGGAAGCCAAATTCATTTGTATTCAAGAATGCAGAACTTGTTCTTAAGCAACTTGTGTCATCTGTTCATGTCAATGCTCAAGTTGCAAATCACGAAATTGCTGGCAGTCATCAGTTCAAAACGCTGTATATGGTAGGAGATAATCCCTTAGTTGACATCAAGGGTGCACGACAGGCTGGCCATCCCTGGTTTTCCATTCTGACAAGGACTGGAGTTTTCAAGGGAAAGGAAAATCATGACGAGTTTCCAGCTGATCTGGTTGTTGATACTGTCGAAGAAGCTGTGGACTATGTTTTGAAAAAGGAGTCGATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

382

Amino Acids

42.32

Weight (kDa)

8.38

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_6 PF13344 45 - 149 3.8e-18 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 277 - 371 4.4e-11 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 708
Acc36I ACCTGC 2 cut(s) 424, 520
AccI GTMKAC 1 cut(s) 821
AccII CGCG 1 cut(s) 476
AciI CCGC 2 cut(s) 474, 587
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 2 cut(s) 374, 841
AcuI CTGAAG 1 cut(s) 184
AcvI CACGTG 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 242, 597, 701
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 182, 438
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 243
AgeI ACCGGT 1 cut(s) 656
AgsI TTSAA 6 cut(s) 459, 485, 856, 949, 1051, 1129
AjnI CCWGG 4 cut(s) 230, 667, 726, 1016
AluBI AGCT 6 cut(s) 97, 304, 388, 764, 1082, 1112
AluI AGCT 6 cut(s) 97, 304, 388, 764, 1082, 1112
Alw21I GWGCWC 2 cut(s) 289, 1000
Alw26I GTCTC 1 cut(s) 552
Alw44I GTGCAC 1 cut(s) 996
AlwI GGATC 1 cut(s) 488
AlwNI CAGNNNCTG 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 2 cut(s) 195, 1009
ApaLI GTGCAC 1 cut(s) 996
ApeKI GCWGC 3 cut(s) 94, 419, 731
ApoI RAATTY 2 cut(s) 374, 841
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 3 cut(s) 25, 579, 870
AspLEI GCGC 2 cut(s) 551, 709
AsuHPI GGTGA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 192
BaeGI GKGCMC 1 cut(s) 1000
BalI TGGCCA 1 cut(s) 1011
BbrPI CACGTG 1 cut(s) 290
BbsI GAAGAC 2 cut(s) 211, 656
Bbv12I GWGCWC 2 cut(s) 289, 1000
BbvI GCAGC 3 cut(s) 106, 406, 718
BccI CCATC 3 cut(s) 185, 794, 1020
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 4 cut(s) 232, 669, 728, 1018
BcoDI GTCTC 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 875
BfuAI ACCTGC 2 cut(s) 424, 520
BisI GCNGC 5 cut(s) 95, 420, 474, 588, 732
BlsI GCNGC 5 cut(s) 96, 421, 475, 589, 733
Bme1390I CCNGG 4 cut(s) 232, 669, 728, 1018
BmeT110I CYCGRG 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 4 cut(s) 232, 669, 728, 1018
BmrI ACTGGG 1 cut(s) 767
BmuI ACTGGG 1 cut(s) 767
BpiI GAAGAC 2 cut(s) 211, 656
BplI GAGNNNNNCTC 1 cut(s) 1127
BpmI CTGGAG 2 cut(s) 836, 1062
BpuEI CTTGAG 1 cut(s) 894
BsaAI YACGTR 2 cut(s) 244, 290
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 230, 1016
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 438
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 2 cut(s) 762, 1045
Bse21I CCTNAGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 4 cut(s) 232, 669, 728, 1018
BseDI CCNNGG 2 cut(s) 230, 1016
BseGI GGATG 2 cut(s) 226, 1012
BseLI CCNNNNNNNGG 2 cut(s) 182, 438
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 22, 206, 735
BseNI ACTGG 2 cut(s) 762, 1045
BseRI GAGGAG 2 cut(s) 46, 91
BseSI GKGCMC 1 cut(s) 1000
BseXI GCAGC 3 cut(s) 106, 406, 718
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 2 cut(s) 197, 1011
BshTI ACCGGT 1 cut(s) 656
BsiHKAI GWGCWC 2 cut(s) 289, 1000
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 1 cut(s) 657
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 2 cut(s) 415, 638
BslI CCNNNNNNNGG 2 cut(s) 182, 438
BsmAI GTCTC 1 cut(s) 552
BsmFI GGGAC 2 cut(s) 415, 638
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 2 cut(s) 197, 1011
Bso31I GGTCTC 1 cut(s) 552
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 2 cut(s) 289, 1000
Bsp143I GATC 3 cut(s) 493, 1084, 1140
BspACI CCGC 2 cut(s) 474, 587
BspANI GGCC 2 cut(s) 197, 1011
BspCNI CTCAG 3 cut(s) 21, 205, 736
BspFNI CGCG 1 cut(s) 476
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 1 cut(s) 187
BspMI ACCTGC 2 cut(s) 424, 520
BspPI GGATC 1 cut(s) 488
BspTI CTTAAG 1 cut(s) 875
BspTNI GGTCTC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 132
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 2 cut(s) 762, 1045
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 2 cut(s) 230, 1016
BssMI GATC 3 cut(s) 493, 1084, 1140
BssNAI GTATAC 1 cut(s) 822
BssNI GRCGYC 1 cut(s) 153
Bst1107I GTATAC 1 cut(s) 822
Bst2UI CCWGG 4 cut(s) 232, 669, 728, 1018
Bst4CI ACNGT 2 cut(s) 699, 1102
BstACI GRCGYC 1 cut(s) 153
BstAFI CTTAAG 1 cut(s) 875
BstBAI YACGTR 2 cut(s) 244, 290
BstC8I GCNNGC 4 cut(s) 342, 478, 934, 1009
BstDEI CTNAG 6 cut(s) 8, 192, 599, 744, 979, 1146
BstF5I GGATG 2 cut(s) 226, 1012
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 2 cut(s) 551, 709
BstKTI GATC 3 cut(s) 496, 1087, 1143
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 3 cut(s) 493, 1084, 1140
BstMWI GCNNNNNNNGC 3 cut(s) 728, 914, 1004
BstNI CCWGG 4 cut(s) 232, 669, 728, 1018
BstSCI CCNGG 4 cut(s) 230, 667, 726, 1016
BstSLI GKGCMC 1 cut(s) 1000
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 3 cut(s) 106, 406, 718
BstV2I GAAGAC 2 cut(s) 211, 656
BstZ17I GTATAC 1 cut(s) 822
Bsu36I CCTNAGG 1 cut(s) 192
BsuRI GGCC 2 cut(s) 197, 1011
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 226, 1012
BveI ACCTGC 2 cut(s) 424, 520
Cac8I GCNNGC 4 cut(s) 342, 478, 934, 1009
CaiI CAGNNNCTG 1 cut(s) 637
CciI TCATGA 1 cut(s) 1066
CfoI GCGC 2 cut(s) 551, 709
Cfr10I RCCGGY 1 cut(s) 656
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 241, 596, 700
CspAI ACCGGT 1 cut(s) 656
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 5 cut(s) 427, 710, 809, 899, 1067
CviQI GTAC 3 cut(s) 241, 596, 700
DdeI CTNAG 6 cut(s) 8, 192, 599, 744, 979, 1146
DpnI GATC 3 cut(s) 495, 1086, 1142
DpnII GATC 3 cut(s) 493, 1084, 1140
EaeI YGGCCR 1 cut(s) 1009
Eco147I AGGCCT 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 552
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 184
Eco72I CACGTG 1 cut(s) 290
Eco81I CCTNAGG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 272
EcoRII CCWGG 4 cut(s) 230, 667, 726, 1016
FaeI CATG 5 cut(s) 430, 713, 812, 902, 1070
FaqI GGGAC 2 cut(s) 415, 638
FatI CATG 5 cut(s) 426, 709, 808, 898, 1066
FblI GTMKAC 1 cut(s) 821
Fnu4HI GCNGC 5 cut(s) 95, 420, 474, 588, 732
FokI GGATG 2 cut(s) 213, 999
Fsp4HI GCNGC 5 cut(s) 95, 420, 474, 588, 732
FspI TGCGCA 1 cut(s) 708
GlaI GCGC 2 cut(s) 550, 708
GluI GCNGC 5 cut(s) 95, 420, 474, 588, 732
GsuI CTGGAG 2 cut(s) 836, 1062
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 2 cut(s) 197, 1011
HapII CCGG 1 cut(s) 657
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 551, 709
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 5 cut(s) 430, 713, 812, 902, 1070
Hin6I GCGC 2 cut(s) 549, 707
HinP1I GCGC 2 cut(s) 549, 707
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HinfI GANTC 8 cut(s) 12, 218, 235, 275, 605, 782, 798, 1136
HpaII CCGG 1 cut(s) 657
HphI GGTGA 1 cut(s) 315
Hpy166II GTNNAC 5 cut(s) 519, 822, 985, 998, 1117
Hpy188I TCNGA 8 cut(s) 11, 182, 203, 543, 557, 745, 770, 1032
Hpy188III TCNNGA 8 cut(s) 16, 250, 272, 674, 815, 856, 923, 1067
Hpy8I GTNNAC 5 cut(s) 519, 822, 985, 998, 1117
Hpy99I CGWCG 3 cut(s) 152, 158, 218
HpyAV CCTTC 4 cut(s) 126, 208, 336, 750
HpyCH4III ACNGT 2 cut(s) 699, 1102
HpyCH4IV ACGT 2 cut(s) 243, 289
HpyCH4V TGCA 6 cut(s) 340, 722, 776, 863, 917, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 728, 914, 1004
HpyF3I CTNAG 6 cut(s) 8, 192, 599, 744, 979, 1146
HpySE526I ACGT 2 cut(s) 243, 289
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 5 cut(s) 430, 713, 812, 902, 1070
HspAI GCGC 2 cut(s) 549, 707
Kzo9I GATC 3 cut(s) 493, 1084, 1140
LmnI GCTCC 4 cut(s) 89, 191, 618, 761
Lsp1109I GCAGC 3 cut(s) 106, 406, 718
MaeII ACGT 2 cut(s) 243, 289
MaeIII GTNAC 1 cut(s) 641
MalI GATC 3 cut(s) 495, 1086, 1142
MboI GATC 3 cut(s) 493, 1084, 1140
MboII GAAGA 6 cut(s) 58, 111, 114, 216, 656, 1118
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 289, 1000
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 7 cut(s) 362, 374, 459, 678, 771, 841, 927
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 284, 1145
MnlI CCTC 8 cut(s) 17, 67, 98, 112, 176, 201, 254, 816
Mox20I TGGCCA 1 cut(s) 1011
MroXI GAANNNNTTC 3 cut(s) 25, 579, 870
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 5 cut(s) 296, 357, 650, 792, 876
MslI CAYNNNNRTG 2 cut(s) 807, 903
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 1082
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 4 cut(s) 232, 669, 728, 1018
MunI CAATTG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 4 cut(s) 232, 669, 728, 1018
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 3 cut(s) 728, 914, 1004
NdeII GATC 3 cut(s) 493, 1084, 1140
NlaIII CATG 5 cut(s) 430, 713, 812, 902, 1070
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 641
NsbI TGCGCA 1 cut(s) 708
PagI TCATGA 1 cut(s) 1066
PceI AGGCCT 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 865
PdmI GAANNNNTTC 3 cut(s) 25, 579, 870
PfeI GAWTC 6 cut(s) 12, 218, 235, 605, 782, 798
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 5 cut(s) 96, 421, 475, 589, 733
PleI GAGTC 2 cut(s) 283, 1144
PmaCI CACGTG 1 cut(s) 290
PmlI CACGTG 1 cut(s) 290
PpsI GAGTC 2 cut(s) 283, 1144
Ppu21I YACGTR 2 cut(s) 244, 290
Psp6I CCWGG 4 cut(s) 230, 667, 726, 1016
PspCI CACGTG 1 cut(s) 290
PspGI CCWGG 4 cut(s) 230, 667, 726, 1016
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 637
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 3 cut(s) 242, 597, 701
RsaNI GTAC 3 cut(s) 241, 596, 700
RseI CAYNNNNRTG 2 cut(s) 807, 903
SaqAI TTAA 5 cut(s) 296, 357, 650, 792, 876
SatI GCNGC 5 cut(s) 95, 420, 474, 588, 732
Sau3AI GATC 3 cut(s) 493, 1084, 1140
SchI GAGTC 2 cut(s) 284, 1145
ScrFI CCNGG 4 cut(s) 232, 669, 728, 1018
SduI GDGCHC 2 cut(s) 289, 1000
SmiMI CAYNNNNRTG 2 cut(s) 807, 903
SmlI CTYRAG 2 cut(s) 875, 909
SmoI CTYRAG 2 cut(s) 875, 909
Sse9I AATT 7 cut(s) 362, 374, 459, 678, 771, 841, 927
SseBI AGGCCT 1 cut(s) 197
SsiI CCGC 2 cut(s) 474, 587
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 4 cut(s) 230, 667, 726, 1016
TaaI ACNGT 2 cut(s) 699, 1102
TaiI ACGT 2 cut(s) 246, 292
TaqI TCGA 5 cut(s) 141, 147, 612, 1104, 1139
TasI AATT 7 cut(s) 362, 374, 459, 678, 771, 841, 927
TatI WGTACW 2 cut(s) 595, 699
TauI GCSGC 2 cut(s) 476, 590
TfiI GAWTC 6 cut(s) 12, 218, 235, 605, 782, 798
Tru1I TTAA 5 cut(s) 296, 357, 650, 792, 876
Tru9I TTAA 5 cut(s) 296, 357, 650, 792, 876
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 3 cut(s) 94, 419, 731
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 2 cut(s) 834, 887
TspGWI ACGGA 1 cut(s) 228
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 875
VneI GTGCAC 1 cut(s) 996
XapI RAATTY 2 cut(s) 374, 841
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 821
XmnI GAANNNNTTC 3 cut(s) 25, 579, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.