pycom07g17450

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001386
Physical Location & Seq
Forward (+)
20019635 .. 20023657
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17450.12

Sequence Viewer

Length: 1185 bp
ATGACGCCGATGAGATACCGCGCGGTCTATCGGATTTCTCAGTACAAAAACAGAGCGCCATTTTTCTTCCAATTCCGTCCCTACTCTCAGTCCAATTATCAACAATCCAGGCCGGAACGGTCTTCGTTTGGGATTGCGTTCGACATCGACGGGGTTATTCTCCGCGGCCGGGCTCCGGTGGGAGGGTCGCCTCGAGCTTTGAGGAAACTGTACGGACATTCAGGTACTTTGAAGGTTCCCTTTGTGTTTTTGACCAACGGAGGTGGCGTCCCTGAATCAAGACGAGCAACTGAGTTAACTGAAATTTTGGGAGTTAACATCCTGCCTTCTCAGGTTGTCCAGGGTCACACACCTTTTCAAAATTTGTTGACAAGATATGAAAATGAACTCATTGTTGCTCTGGGAAAAGGGGAACCGGCTCTTGTAATGTCTGAGTATGGTTTCAAAAAGGTTCTCTCATTAGATGAGTATGCATCGCACTTCAAGAATATTGATCCTGTATCCCAATATAAGATGTGGAGAACTAAGCAGACATCGGATTGCAGTAGCCAACACAAGGAGTCTGTGCCGAGATATGATGTTTATTCTGACAGGGTTAGTGCAGCTTTTGTTGTCAGTGATCCTGTGGATTGGGGTAGGGATATTCAGGTTCTCTGTGACATTCTAAGATCTGGAGGGCTTCCTGGACAAGAGAACAGGCATCAACCACCTCTATATTTTGCCGCAGATGATCTTGAGTATCAGGCTGCATTTCCTTCTGAGCGACTTGGAATGGGTGCTTTCAGAATTGCACTAGAAAGCATCTTCAACCGAATTCACCATGATGCTCTGGAATATGTATCTTTTGGGAAGCCAAATCCGTTTGTATTTGAGAACACTGAAACTCTGTTGGCACAGCTTCAACCATCTCATTGTGATAATGATATTACAACTGCTGGAGACTCTCCGTCACATGCTTTCAAAACCCTTTATATGATTGGCGACAACCCTTCAGTTGATGTCAAAGGTGCTCGGCAGGCTGGACATCCTTGGTTTTCTATTTTGACGAGGACCGGCGTTTTTAAGGGCAAAGATAATCATGCTGAGTTTCCAGCAGACCTGGTTGTAGATACTGTACAAGAGGCAGTGGACTACATTCTGAAGAGGGAAGGAACTTCTCTTGCGTTTTCCCCTACTCTCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

395

Amino Acids

44.17

Weight (kDa)

6.84

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 21, 23, 165
AciI CCGC 5 cut(s) 19, 23, 163, 165, 723
AclWI GGATC 2 cut(s) 488, 614
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 3 cut(s) 303, 361, 813
AcuI CTGAAG 2 cut(s) 975, 1160
AcyI GRCGYC 2 cut(s) 5, 267
AfaI GTAC 4 cut(s) 44, 212, 226, 1116
AfiI CCNNNNNNNGG 4 cut(s) 169, 175, 182, 556
AgsI TTSAA 7 cut(s) 232, 359, 445, 484, 808, 902, 961
AhdI GACNNNNNGTC 1 cut(s) 946
AjnI CCWGG 4 cut(s) 107, 339, 682, 1098
AluBI AGCT 3 cut(s) 197, 605, 898
AluI AGCT 3 cut(s) 197, 605, 898
Alw21I GWGCWC 1 cut(s) 1012
Alw26I GTCTC 1 cut(s) 933
AlwI GGATC 2 cut(s) 488, 614
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 2 cut(s) 110, 166
ApeKI GCWGC 2 cut(s) 602, 746
ApoI RAATTY 3 cut(s) 303, 361, 813
AspLEI GCGC 2 cut(s) 23, 58
AspS9I GGNCC 1 cut(s) 1050
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 1 cut(s) 1050
BanII GRGCYC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 1012
BbvI GCAGC 2 cut(s) 614, 733
BccI CCATC 1 cut(s) 913
BciT130I CCWGG 4 cut(s) 109, 341, 684, 1100
BciVI GTATCC 1 cut(s) 511
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 933
BfaI CTAG 1 cut(s) 794
BfoI RGCGCY 1 cut(s) 59
BfuI GTATCC 1 cut(s) 511
BglII AGATCT 1 cut(s) 668
BisI GCNGC 4 cut(s) 166, 603, 723, 747
BlsI GCNGC 4 cut(s) 167, 604, 724, 748
Bme1390I CCNGG 5 cut(s) 109, 170, 341, 684, 1100
Bme18I GGWCC 1 cut(s) 1050
BmeRI GACNNNNNGTC 1 cut(s) 946
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 1050
BmiI GGNNCC 3 cut(s) 174, 237, 414
BmrFI CCNGG 5 cut(s) 109, 170, 341, 684, 1100
BmsI GCATC 4 cut(s) 482, 709, 810, 814
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 2 cut(s) 693, 957
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 1 cut(s) 170
BsaHI GRCGYC 2 cut(s) 5, 267
BsaJI CCNNGG 3 cut(s) 163, 340, 1028
BsaWI WCCGGW 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 175, 182, 556
Bse118I RCCGGY 2 cut(s) 415, 1052
BseBI CCWGG 4 cut(s) 109, 341, 684, 1100
BseDI CCNNGG 3 cut(s) 163, 340, 1028
BseGI GGATG 2 cut(s) 318, 1024
BseLI CCNNNNNNNGG 4 cut(s) 169, 175, 182, 556
BseMII CTCAG 7 cut(s) 53, 101, 282, 344, 423, 750, 1074
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 2 cut(s) 614, 733
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 3 cut(s) 21, 23, 165
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 2 cut(s) 112, 168
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 1012
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 5 cut(s) 113, 169, 176, 416, 1053
BslFI GGGAC 2 cut(s) 63, 254
BslI CCNNNNNNNGG 4 cut(s) 169, 175, 182, 556
BsmAI GTCTC 1 cut(s) 933
BsmFI GGGAC 2 cut(s) 63, 254
BsnI GGCC 2 cut(s) 112, 168
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 175, 1012
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 4 cut(s) 493, 619, 668, 730
BspACI CCGC 5 cut(s) 19, 23, 163, 165, 723
BspANI GGCC 2 cut(s) 112, 168
BspCNI CTCAG 7 cut(s) 52, 100, 283, 343, 424, 751, 1075
BspFNI CGCG 3 cut(s) 21, 23, 165
BspLI GGNNCC 3 cut(s) 174, 237, 414
BspPI GGATC 2 cut(s) 488, 614
BsrFI RCCGGY 2 cut(s) 415, 1052
BsrGI TGTACA 1 cut(s) 1114
BssAI RCCGGY 2 cut(s) 415, 1052
BssECI CCNNGG 3 cut(s) 163, 340, 1028
BssMI GATC 4 cut(s) 493, 619, 668, 730
BssNI GRCGYC 2 cut(s) 5, 267
BssT1I CCWWGG 1 cut(s) 1028
Bst2UI CCWGG 4 cut(s) 109, 341, 684, 1100
Bst4CI ACNGT 3 cut(s) 120, 210, 1114
Bst6I CTCTTC 1 cut(s) 1136
BstACI GRCGYC 2 cut(s) 5, 267
BstAUI TGTACA 1 cut(s) 1114
BstC8I GCNNGC 1 cut(s) 1017
BstDEI CTNAG 9 cut(s) 39, 87, 291, 330, 432, 525, 665, 759, 1083
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 2 cut(s) 318, 1024
BstFNI CGCG 3 cut(s) 21, 23, 165
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 2 cut(s) 23, 58
BstKTI GATC 4 cut(s) 496, 622, 671, 733
BstMAI GTCTC 1 cut(s) 933
BstMBI GATC 4 cut(s) 493, 619, 668, 730
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 1016
BstNI CCWGG 4 cut(s) 109, 341, 684, 1100
BstNSI RCATGY 1 cut(s) 956
BstSCI CCNGG 5 cut(s) 107, 168, 339, 682, 1098
BstUI CGCG 3 cut(s) 21, 23, 165
BstV1I GCAGC 2 cut(s) 614, 733
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZI CGGCCG 1 cut(s) 166
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 2 cut(s) 112, 168
BtgI CCRYGG 1 cut(s) 163
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 2 cut(s) 318, 1024
BtsI GCAGTG 1 cut(s) 1131
BtsIMutI CAGTG 3 cut(s) 622, 876, 1131
Cac8I GCNNGC 1 cut(s) 1017
CfoI GCGC 2 cut(s) 23, 58
Cfr10I RCCGGY 2 cut(s) 415, 1052
Cfr13I GGNCC 1 cut(s) 1050
Cfr42I CCGCGG 1 cut(s) 166
CseI GACGC 2 cut(s) 13, 256
CsiI ACCWGGT 1 cut(s) 1098
Csp6I GTAC 4 cut(s) 43, 211, 225, 1115
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 3 cut(s) 821, 953, 1079
CviQI GTAC 4 cut(s) 43, 211, 225, 1115
DdeI CTNAG 9 cut(s) 39, 87, 291, 330, 432, 525, 665, 759, 1083
DpnI GATC 4 cut(s) 495, 621, 670, 732
DpnII GATC 4 cut(s) 493, 619, 668, 730
DriI GACNNNNNGTC 1 cut(s) 946
EaeI YGGCCR 1 cut(s) 166
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 1136
Eam1105I GACNNNNNGTC 1 cut(s) 946
EarI CTCTTC 1 cut(s) 1136
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 1028
Eco24I GRGCYC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 1050
Eco52I CGGCCG 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 975, 1160
Eco88I CYCGRG 1 cut(s) 192
EcoRI GAATTC 1 cut(s) 813
EcoRII CCWGG 4 cut(s) 107, 339, 682, 1098
EcoT14I CCWWGG 1 cut(s) 1028
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 1028
FaeI CATG 3 cut(s) 824, 956, 1082
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 2 cut(s) 63, 254
FatI CATG 3 cut(s) 820, 952, 1078
Fnu4HI GCNGC 4 cut(s) 166, 603, 723, 747
FokI GGATG 2 cut(s) 305, 1011
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 4 cut(s) 166, 603, 723, 747
FspBI CTAG 1 cut(s) 794
GlaI GCGC 2 cut(s) 22, 57
GluI GCNGC 4 cut(s) 166, 603, 723, 747
GsuI CTGGAG 2 cut(s) 693, 957
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 2 cut(s) 112, 168
HapII CCGG 5 cut(s) 113, 169, 176, 416, 1053
HgaI GACGC 2 cut(s) 13, 256
HhaI GCGC 2 cut(s) 23, 58
Hin1I GRCGYC 2 cut(s) 5, 267
Hin1II CATG 3 cut(s) 824, 956, 1082
Hin6I GCGC 2 cut(s) 21, 56
HinP1I GCGC 2 cut(s) 21, 56
HincII GTYRAC 3 cut(s) 297, 316, 369
HindII GTYRAC 3 cut(s) 297, 316, 369
HinfI GANTC 3 cut(s) 275, 560, 941
HpaI GTTAAC 2 cut(s) 297, 316
HpaII CCGG 5 cut(s) 113, 169, 176, 416, 1053
HphI GGTGA 1 cut(s) 809
Hpy166II GTNNAC 4 cut(s) 297, 316, 369, 1129
Hpy188I TCNGA 7 cut(s) 33, 433, 538, 589, 760, 785, 1140
Hpy188III TCNNGA 5 cut(s) 279, 484, 672, 734, 830
Hpy8I GTNNAC 4 cut(s) 297, 316, 369, 1129
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 226, 336, 765, 999, 1142
HpyCH4III ACNGT 3 cut(s) 120, 210, 1114
HpyCH4V TGCA 5 cut(s) 473, 543, 602, 749, 791
HpyF10VI GCNNNNNNNGC 1 cut(s) 1016
HpyF3I CTNAG 9 cut(s) 39, 87, 291, 330, 432, 525, 665, 759, 1083
Hsp92I GRCGYC 2 cut(s) 5, 267
Hsp92II CATG 3 cut(s) 824, 956, 1082
HspAI GCGC 2 cut(s) 21, 56
KspAI GTTAAC 2 cut(s) 297, 316
KspI CCGCGG 1 cut(s) 166
Kzo9I GATC 4 cut(s) 493, 619, 668, 730
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 2 cut(s) 614, 733
LweI GCATC 4 cut(s) 482, 709, 810, 814
MabI ACCWGGT 1 cut(s) 1098
MaeI CTAG 1 cut(s) 794
MaeIII GTNAC 3 cut(s) 344, 656, 948
MalI GATC 4 cut(s) 495, 621, 670, 732
MboI GATC 4 cut(s) 493, 619, 668, 730
MboII GAAGA 4 cut(s) 58, 114, 796, 1153
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 2 cut(s) 175, 1012
MluCI AATT 6 cut(s) 71, 94, 303, 361, 786, 813
MlyI GAGTC 2 cut(s) 569, 935
MnlI CCTC 9 cut(s) 176, 195, 201, 254, 668, 720, 1041, 1114, 1137
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 3 cut(s) 296, 315, 1062
MslI CAYNNNNRTG 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 5 cut(s) 113, 169, 176, 416, 1053
MspR9I CCNGG 5 cut(s) 109, 170, 341, 684, 1100
MvaI CCWGG 4 cut(s) 109, 341, 684, 1100
MvnI CGCG 3 cut(s) 21, 23, 165
MwoI GCNNNNNNNGC 1 cut(s) 1016
NciI CCSGG 1 cut(s) 170
NdeII GATC 4 cut(s) 493, 619, 668, 730
NlaIII CATG 3 cut(s) 824, 956, 1082
NlaIV GGNNCC 3 cut(s) 174, 237, 414
NmeAIII GCCGAG 2 cut(s) 594, 991
NmuCI GTSAC 3 cut(s) 344, 656, 948
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 1 cut(s) 956
PaeR7I CTCGAG 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 682
PkrI GCNGC 4 cut(s) 167, 604, 724, 748
PleI GAGTC 2 cut(s) 568, 935
PpsI GAGTC 2 cut(s) 568, 935
Psp6I CCWGG 4 cut(s) 107, 339, 682, 1098
PspGI CCWGG 4 cut(s) 107, 339, 682, 1098
PspN4I GGNNCC 3 cut(s) 174, 237, 414
PspPI GGNCC 1 cut(s) 1050
PspXI VCTCGAGB 1 cut(s) 192
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 4 cut(s) 44, 212, 226, 1116
RsaNI GTAC 4 cut(s) 43, 211, 225, 1115
RseI CAYNNNNRTG 1 cut(s) 822
SacII CCGCGG 1 cut(s) 166
SaqAI TTAA 3 cut(s) 296, 315, 1062
SatI GCNGC 4 cut(s) 166, 603, 723, 747
Sau3AI GATC 4 cut(s) 493, 619, 668, 730
Sau96I GGNCC 1 cut(s) 1050
SchI GAGTC 2 cut(s) 569, 935
ScrFI CCNGG 5 cut(s) 109, 170, 341, 684, 1100
SduI GDGCHC 2 cut(s) 175, 1012
SexAI ACCWGGT 1 cut(s) 1098
SfaNI GCATC 4 cut(s) 482, 709, 810, 814
Sfr274I CTCGAG 1 cut(s) 192
Sfr303I CCGCGG 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 166
SinI GGWCC 1 cut(s) 1050
SlaI CTCGAG 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 822
SmlI CTYRAG 2 cut(s) 192, 734
SmoI CTYRAG 2 cut(s) 192, 734
Sse9I AATT 6 cut(s) 71, 94, 303, 361, 786, 813
SsiI CCGC 5 cut(s) 19, 23, 163, 165, 723
SspI AATATT 1 cut(s) 490
SspMI CTAG 1 cut(s) 794
StyD4I CCNGG 5 cut(s) 107, 168, 339, 682, 1098
StyI CCWWGG 1 cut(s) 1028
TaaI ACNGT 3 cut(s) 120, 210, 1114
TaqI TCGA 3 cut(s) 141, 147, 193
TasI AATT 6 cut(s) 71, 94, 303, 361, 786, 813
TatI WGTACW 2 cut(s) 42, 1114
TauI GCSGC 2 cut(s) 168, 725
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 3 cut(s) 296, 315, 1062
Tru9I TTAA 3 cut(s) 296, 315, 1062
TscAI CASTG 3 cut(s) 622, 883, 1131
TseFI GTSAC 3 cut(s) 344, 656, 948
TseI GCWGC 2 cut(s) 602, 746
Tsp45I GTSAC 3 cut(s) 344, 656, 948
TspDTI ATGAA 2 cut(s) 393, 399
TspGWI ACGGA 5 cut(s) 65, 228, 273, 849, 936
TspRI CASTG 3 cut(s) 622, 883, 1131
VpaK11BI GGWCC 1 cut(s) 1050
XapI RAATTY 3 cut(s) 303, 361, 813
XceI RCATGY 1 cut(s) 956
XhoI CTCGAG 1 cut(s) 192
XspI CTAG 1 cut(s) 794
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.