Rmu_sc0003709.1_g000009

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003709.1
OG0001386
Physical Location & Seq
Forward (+)
24760 .. 27492
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003709.1_g000009.1.cds

Sequence Viewer

Length: 1149 bp
atgagcctcagaatcgtgagaaaggcttcacaaacccaaaacccgaagcaactctcctccattttcttgtccaaaatcacacgctccctctcgcagctctcctctcaatccaaaccttcttcttcgtttgccattgctttcgacatcgacggcgtcgtttcccgtagcgatgttcccatcggaggctcccctcaggccttcagaagactatacgacgattctggtcatcccaggattccgtacgtgttcctgaccaatggaggtgggtttcccgagtccaaaagagcatgtgagttaagtgagcttttgggtgtcaaggtttcaccttcacaggttttgcaggctcatacaccttttaagcaattagtgagcaaatttgagaatgagcttattgttgctgtgggaaaaggagaacctgctgctgtcatgtcccattacgggtttagaaatgtggtttcaattgatgactatgccgcgtgctttgaaaacattgatccattagcaccacataagaagtggacaactaagcaggttggtaatccgaatagcgctatcagagaccttgttcgcacagaaagagttcaggcggcgtttgttcttagtgattctgtcgattggagcagggacattcaggttctgtgtgacattttaagaaccggtggtcttcctggtcgggaaattggacatcaaccacaactgtactttgcgcatgatgaccttgcataccaggctgcttttccttctgagcgttttggactgggagctttcagaattgcattggaatccatctttaacagaatccaccctcatgctctggagtatacaacttttgggaagccaaattcatttgtattcaagaatgcagaacttgttcttaagcaacttgtgtcatctgttcatgtcaatgctcaagttgcaaatcacgaaattgctggcagtcatcagttcaaaacgctgtatatggtaggagataatcccttagttgacatcaagggtgcacgacaggctggccatccctggttttccattctgacaaggactggagttttcaagggaaaggaaaatcatgacgagtttccagctgatctggttgttgatactgtcgaagaagctgtggactatgttttgaaaaaggagtcgatctcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_6 Hydrolase_like

Protein Analysis

382

Amino Acids

42.24

Weight (kDa)

7.78

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 708
Acc36I ACCTGC 2 cut(s) 424, 520
AccI GTMKAC 1 cut(s) 821
AccII CGCG 1 cut(s) 476
AciI CCGC 2 cut(s) 474, 587
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 2 cut(s) 374, 841
AcuI CTGAAG 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 2 cut(s) 242, 701
AfeI AGCGCT 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 182, 438
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 243
AgeI ACCGGT 1 cut(s) 656
AgsI TTSAA 6 cut(s) 459, 485, 856, 949, 1051, 1129
AjnI CCWGG 4 cut(s) 230, 667, 726, 1016
AluBI AGCT 6 cut(s) 97, 304, 388, 764, 1082, 1112
AluI AGCT 6 cut(s) 97, 304, 388, 764, 1082, 1112
Alw21I GWGCWC 1 cut(s) 1000
Alw26I GTCTC 1 cut(s) 552
Alw44I GTGCAC 1 cut(s) 996
AlwI GGATC 1 cut(s) 488
AlwNI CAGNNNCTG 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 550
AoxI GGCC 2 cut(s) 195, 1009
ApaLI GTGCAC 1 cut(s) 996
ApeKI GCWGC 3 cut(s) 94, 419, 731
ApoI RAATTY 2 cut(s) 374, 841
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 3 cut(s) 25, 579, 870
AspLEI GCGC 2 cut(s) 551, 709
AsuHPI GGTGA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 192
BaeGI GKGCMC 1 cut(s) 1000
BalI TGGCCA 1 cut(s) 1011
BbsI GAAGAC 2 cut(s) 211, 656
Bbv12I GWGCWC 1 cut(s) 1000
BbvI GCAGC 3 cut(s) 106, 406, 718
BccI CCATC 3 cut(s) 185, 794, 1020
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 4 cut(s) 232, 669, 728, 1018
BcoDI GTCTC 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 875
BfuAI ACCTGC 2 cut(s) 424, 520
BisI GCNGC 5 cut(s) 95, 420, 474, 588, 732
BlsI GCNGC 5 cut(s) 96, 421, 475, 589, 733
Bme1390I CCNGG 4 cut(s) 232, 669, 728, 1018
BmeT110I CYCGRG 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 4 cut(s) 232, 669, 728, 1018
BmrI ACTGGG 1 cut(s) 767
BmuI ACTGGG 1 cut(s) 767
BpiI GAAGAC 2 cut(s) 211, 656
BplI GAGNNNNNCTC 1 cut(s) 1127
BpmI CTGGAG 2 cut(s) 836, 1062
BpuEI CTTGAG 1 cut(s) 894
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 230, 1016
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 438
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 2 cut(s) 762, 1045
Bse21I CCTNAGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 4 cut(s) 232, 669, 728, 1018
BseDI CCNNGG 2 cut(s) 230, 1016
BseGI GGATG 2 cut(s) 226, 1012
BseLI CCNNNNNNNGG 2 cut(s) 182, 438
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 22, 206, 735
BseNI ACTGG 2 cut(s) 762, 1045
BseRI GAGGAG 2 cut(s) 46, 91
BseSI GKGCMC 1 cut(s) 1000
BseXI GCAGC 3 cut(s) 106, 406, 718
Bsh1236I CGCG 1 cut(s) 476
BshFI GGCC 2 cut(s) 197, 1011
BshTI ACCGGT 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 1000
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 1 cut(s) 657
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 2 cut(s) 415, 638
BslI CCNNNNNNNGG 2 cut(s) 182, 438
BsmAI GTCTC 1 cut(s) 552
BsmFI GGGAC 2 cut(s) 415, 638
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 2 cut(s) 197, 1011
Bso31I GGTCTC 1 cut(s) 552
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 1000
Bsp143I GATC 3 cut(s) 493, 1084, 1140
BspACI CCGC 2 cut(s) 474, 587
BspANI GGCC 2 cut(s) 197, 1011
BspCNI CTCAG 3 cut(s) 21, 205, 736
BspFNI CGCG 1 cut(s) 476
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 1 cut(s) 187
BspMI ACCTGC 2 cut(s) 424, 520
BspPI GGATC 1 cut(s) 488
BspTI CTTAAG 1 cut(s) 875
BspTNI GGTCTC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 132
BsrFI RCCGGY 1 cut(s) 656
BsrI ACTGG 2 cut(s) 762, 1045
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 2 cut(s) 230, 1016
BssMI GATC 3 cut(s) 493, 1084, 1140
BssNAI GTATAC 1 cut(s) 822
BssNI GRCGYC 1 cut(s) 153
Bst1107I GTATAC 1 cut(s) 822
Bst2UI CCWGG 4 cut(s) 232, 669, 728, 1018
Bst4CI ACNGT 2 cut(s) 699, 1102
BstACI GRCGYC 1 cut(s) 153
BstAFI CTTAAG 1 cut(s) 875
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 342, 478, 934, 1009
BstDEI CTNAG 7 cut(s) 8, 192, 525, 599, 744, 979, 1146
BstF5I GGATG 2 cut(s) 226, 1012
BstFNI CGCG 1 cut(s) 476
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 2 cut(s) 551, 709
BstKTI GATC 3 cut(s) 496, 1087, 1143
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 3 cut(s) 493, 1084, 1140
BstMWI GCNNNNNNNGC 3 cut(s) 728, 914, 1004
BstNI CCWGG 4 cut(s) 232, 669, 728, 1018
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 4 cut(s) 230, 667, 726, 1016
BstSLI GKGCMC 1 cut(s) 1000
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 3 cut(s) 106, 406, 718
BstV2I GAAGAC 2 cut(s) 211, 656
BstZ17I GTATAC 1 cut(s) 822
Bsu36I CCTNAGG 1 cut(s) 192
BsuRI GGCC 2 cut(s) 197, 1011
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 2 cut(s) 226, 1012
BveI ACCTGC 2 cut(s) 424, 520
Cac8I GCNNGC 4 cut(s) 342, 478, 934, 1009
CaiI CAGNNNCTG 1 cut(s) 637
CciI TCATGA 1 cut(s) 1066
CfoI GCGC 2 cut(s) 551, 709
Cfr10I RCCGGY 1 cut(s) 656
CseI GACGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 241, 700
CspAI ACCGGT 1 cut(s) 656
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 6 cut(s) 288, 427, 710, 809, 899, 1067
CviQI GTAC 2 cut(s) 241, 700
DdeI CTNAG 7 cut(s) 8, 192, 525, 599, 744, 979, 1146
DpnI GATC 3 cut(s) 495, 1086, 1142
DpnII GATC 3 cut(s) 493, 1084, 1140
EaeI YGGCCR 1 cut(s) 1009
Eco147I AGGCCT 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 552
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 184
Eco81I CCTNAGG 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 272
EcoRII CCWGG 4 cut(s) 230, 667, 726, 1016
FaeI CATG 6 cut(s) 291, 430, 713, 812, 902, 1070
FaqI GGGAC 2 cut(s) 415, 638
FatI CATG 6 cut(s) 287, 426, 709, 808, 898, 1066
FblI GTMKAC 1 cut(s) 821
Fnu4HI GCNGC 5 cut(s) 95, 420, 474, 588, 732
FokI GGATG 2 cut(s) 213, 999
Fsp4HI GCNGC 5 cut(s) 95, 420, 474, 588, 732
FspI TGCGCA 1 cut(s) 708
GlaI GCGC 2 cut(s) 550, 708
GluI GCNGC 5 cut(s) 95, 420, 474, 588, 732
GsuI CTGGAG 2 cut(s) 836, 1062
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 2 cut(s) 197, 1011
HapII CCGG 1 cut(s) 657
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 551, 709
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 6 cut(s) 291, 430, 713, 812, 902, 1070
Hin6I GCGC 2 cut(s) 549, 707
HinP1I GCGC 2 cut(s) 549, 707
HincII GTYRAC 1 cut(s) 985
HindII GTYRAC 1 cut(s) 985
HinfI GANTC 8 cut(s) 12, 218, 235, 275, 605, 782, 798, 1136
HpaII CCGG 1 cut(s) 657
HphI GGTGA 1 cut(s) 315
Hpy166II GTNNAC 5 cut(s) 519, 822, 985, 998, 1117
Hpy188I TCNGA 8 cut(s) 11, 182, 203, 543, 557, 745, 770, 1032
Hpy188III TCNNGA 8 cut(s) 16, 250, 272, 674, 815, 856, 923, 1067
Hpy8I GTNNAC 5 cut(s) 519, 822, 985, 998, 1117
Hpy99I CGWCG 3 cut(s) 152, 158, 218
HpyAV CCTTC 4 cut(s) 126, 208, 336, 750
HpyCH4III ACNGT 2 cut(s) 699, 1102
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 6 cut(s) 340, 722, 776, 863, 917, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 728, 914, 1004
HpyF3I CTNAG 7 cut(s) 8, 192, 525, 599, 744, 979, 1146
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 6 cut(s) 291, 430, 713, 812, 902, 1070
HspAI GCGC 2 cut(s) 549, 707
Kzo9I GATC 3 cut(s) 493, 1084, 1140
LmnI GCTCC 4 cut(s) 89, 191, 618, 761
Lsp1109I GCAGC 3 cut(s) 106, 406, 718
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 641
MalI GATC 3 cut(s) 495, 1086, 1142
MboI GATC 3 cut(s) 493, 1084, 1140
MboII GAAGA 5 cut(s) 111, 114, 216, 656, 1118
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 1000
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 7 cut(s) 362, 374, 459, 678, 771, 841, 927
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 284, 1145
MnlI CCTC 8 cut(s) 17, 67, 98, 112, 176, 201, 254, 816
Mox20I TGGCCA 1 cut(s) 1011
MroXI GAANNNNTTC 3 cut(s) 25, 579, 870
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 5 cut(s) 296, 357, 650, 792, 876
MslI CAYNNNNRTG 2 cut(s) 807, 903
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 1082
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 4 cut(s) 232, 669, 728, 1018
MunI CAATTG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 4 cut(s) 232, 669, 728, 1018
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 3 cut(s) 728, 914, 1004
NdeII GATC 3 cut(s) 493, 1084, 1140
NlaIII CATG 6 cut(s) 291, 430, 713, 812, 902, 1070
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 641
NsbI TGCGCA 1 cut(s) 708
NspI RCATGY 1 cut(s) 291
PagI TCATGA 1 cut(s) 1066
PceI AGGCCT 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 865
PdmI GAANNNNTTC 3 cut(s) 25, 579, 870
PfeI GAWTC 6 cut(s) 12, 218, 235, 605, 782, 798
Pfl23II CGTACG 1 cut(s) 240
PflFI GACNNNGTC 1 cut(s) 152
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 5 cut(s) 96, 421, 475, 589, 733
PleI GAGTC 2 cut(s) 283, 1144
PpsI GAGTC 2 cut(s) 283, 1144
Ppu21I YACGTR 1 cut(s) 244
Psp6I CCWGG 4 cut(s) 230, 667, 726, 1016
PspGI CCWGG 4 cut(s) 230, 667, 726, 1016
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 637
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 2 cut(s) 242, 701
RsaNI GTAC 2 cut(s) 241, 700
RseI CAYNNNNRTG 2 cut(s) 807, 903
SaqAI TTAA 5 cut(s) 296, 357, 650, 792, 876
SatI GCNGC 5 cut(s) 95, 420, 474, 588, 732
Sau3AI GATC 3 cut(s) 493, 1084, 1140
SchI GAGTC 2 cut(s) 284, 1145
ScrFI CCNGG 4 cut(s) 232, 669, 728, 1018
SduI GDGCHC 1 cut(s) 1000
SmiMI CAYNNNNRTG 2 cut(s) 807, 903
SmlI CTYRAG 2 cut(s) 875, 909
SmoI CTYRAG 2 cut(s) 875, 909
Sse9I AATT 7 cut(s) 362, 374, 459, 678, 771, 841, 927
SseBI AGGCCT 1 cut(s) 197
SsiI CCGC 2 cut(s) 474, 587
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 4 cut(s) 230, 667, 726, 1016
TaaI ACNGT 2 cut(s) 699, 1102
TaiI ACGT 1 cut(s) 246
TaqI TCGA 5 cut(s) 141, 147, 612, 1104, 1139
TasI AATT 7 cut(s) 362, 374, 459, 678, 771, 841, 927
TatI WGTACW 1 cut(s) 699
TauI GCSGC 2 cut(s) 476, 590
TfiI GAWTC 6 cut(s) 12, 218, 235, 605, 782, 798
Tru1I TTAA 5 cut(s) 296, 357, 650, 792, 876
Tru9I TTAA 5 cut(s) 296, 357, 650, 792, 876
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 3 cut(s) 94, 419, 731
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 2 cut(s) 834, 887
TspGWI ACGGA 1 cut(s) 228
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 875
VneI GTGCAC 1 cut(s) 996
XapI RAATTY 2 cut(s) 374, 841
XceI RCATGY 1 cut(s) 291
XcmI CCANNNNNNNNNTGG 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 821
XmnI GAANNNNTTC 3 cut(s) 25, 579, 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.