AT3G59990

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003170
Physical Location & Seq
Reverse (-)
22155705 .. 22158954
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59990.3

Sequence Viewer

Length: 1320 bp
ATGGCGAGCGAAAGTCCTGATGTTGCTGTTGTAGCTCCGGTGGTGGAGAATGGCGGCGCTGAGTCCTCTAATGGTAAAGAGGAACAATTGGAATCTGAGCTTTCGAAGAAGCTTGAGATTGCAGAAGATGGTCAAGAGGAGAACGATGGAGAAGAAGGAAGCAAAGCTGAGACTTCAACGAAGAAGAAGAAGAAGAAAAATAAAAGCAAGAAGAAGAAGGAACTCCCTCAACAGACTGATCCACCTTCAATTCCTGTCGTTGAGCTCTTCCCATCAGGAGAGTTTCCTGAAGGTGAAATCCAAGAGTATAAGGATGATAATCTTTGGAGAACAACATCTGAAGAGAAGAGAGAGCTGGAGCGTTTTGAAAAGCCAATATATAACTCTGTTCGCCGAGCTGCAGAAGTTCATCGCCAGGTTCGTAAATATGTCAGAAGCATAGTGAAGCCTGGAATGTTGATGACTGATATATGTGAGACCCTAGAGAATACTGTTCGTAAGTTGATATCAGAGAATGGTCTTCAAGCTGGTATTGCATTCCCTACAGGATGCTCTTTGAATTGGGTCGCTGCTCATTGGACACCAAACTCTGGAGATAAGACTGTACTTCAGTACGACGATGTTATGAAATTGGACTTTGGAACACATATTGATGGGCATATTATTGACTGTGCATTTACAGTTGCCTTCAACCCTATGTTCGATCCTCTCTTAGCAGCCTCTCGTGAAGCTACGTATACCGGTATCAAGGAAGCTGGGATCGATGTCCGTCTCTGTGATATCGGTGCTGCTATTCAGGAGGTCATGGAGTCTTATGAGGTTGAAATCAACGGAAAGGTCTTCCAAGTTAAAAGTATCCGAAACTTGAATGGTCACAGCATTGGACCCTATCAGATACATGCTGGGAAATCTGTTCCTATCGTAAAAGGAGGCGAGCAGACAAAGATGGAAGAGGGCGAGTTTTATGCCATCGAAACATTTGGATCAACCGGGAAAGGATATGTGAGAGAAGACCTAGAATGTAGCCATTACATGAAGAACTTTGACGCTGGCCACGTCCCCTTGAGGTTGCCTAGAGCAAAACAACTCCTTGCAACCATTAACAAGAATTTCTCGACTCTCGCCTTCTGCAGACGTTATTTGGACCGCATTGGTGAAACCAAATACTTAATGGCTCTAAAGAATCTTTGTGACTCTGGCATTGTTCAGCCGTATCCTCCTCTGTGTGATGTGAAAGGAAGCTATGTATCACAGTTTGAACACACCATTTTACTGCGACCTACTTGCAAAGAAGTTCTCTCCAAGGGAGACGACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0010467 GO:0016485 GO:0019538 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0051604 GO:0071704 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M24

Protein Analysis

439

Amino Acids

49.06

Weight (kDa)

5.52

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 130 - 328 5.5e-27 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 590
AccI GTMKAC 1 cut(s) 737
AciI CCGC 2 cut(s) 54, 1147
AclWI GGATC 4 cut(s) 233, 698, 767, 991
AcoI YGGCCR 1 cut(s) 1051
AcsI RAATTY 1 cut(s) 1108
AcuI CTGAAG 3 cut(s) 309, 360, 593
AfaI GTAC 2 cut(s) 606, 614
AfiI CCNNNNNNNGG 1 cut(s) 590
AgeI ACCGGT 1 cut(s) 740
AgsI TTSAA 9 cut(s) 177, 249, 368, 524, 559, 691, 824, 868, 1259
AjiI CACGTC 1 cut(s) 1057
AjnI CCWGG 2 cut(s) 414, 448
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 4 cut(s) 164, 470, 776, 1302
AlwI GGATC 4 cut(s) 233, 698, 767, 991
AoxI GGCC 1 cut(s) 1051
ApeKI GCWGC 4 cut(s) 398, 569, 716, 788
ApoI RAATTY 1 cut(s) 1108
AsiGI ACCGGT 1 cut(s) 740
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 2 cut(s) 884, 1144
AsuC2I CCSGG 1 cut(s) 991
AsuHPI GGTGA 2 cut(s) 305, 1166
AsuII TTCGAA 1 cut(s) 104
AvaII GGWCC 2 cut(s) 884, 1144
BalI TGGCCA 1 cut(s) 1053
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 3 cut(s) 512, 832, 1017
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 4 cut(s) 385, 556, 728, 775
BccI CCATC 6 cut(s) 122, 140, 280, 647, 940, 977
BceAI ACGGC 1 cut(s) 1195
BcgI CGANNNNNNTGC 4 cut(s) 947, 981, 1266, 1300
BciT130I CCWGG 2 cut(s) 416, 450
BciVI GTATCC 2 cut(s) 866, 1224
BcnI CCSGG 1 cut(s) 991
BcoDI GTCTC 4 cut(s) 164, 470, 776, 1302
BfaI CTAG 3 cut(s) 482, 1016, 1074
BfmI CTRYAG 3 cut(s) 399, 543, 1129
BfoI RGCGCY 1 cut(s) 60
BfuI GTATCC 2 cut(s) 866, 1224
BisI GCNGC 5 cut(s) 55, 399, 570, 717, 789
BlsI GCNGC 5 cut(s) 56, 400, 571, 718, 790
Bme1390I CCNGG 3 cut(s) 416, 450, 991
Bme18I GGWCC 2 cut(s) 884, 1144
BmgBI CACGTC 1 cut(s) 1057
BmgT120I GGNCC 2 cut(s) 884, 1144
BmiI GGNNCC 1 cut(s) 886
BmrFI CCNGG 3 cut(s) 416, 450, 991
BmsI GCATC 1 cut(s) 539
BpiI GAAGAC 3 cut(s) 512, 832, 1017
BpmI CTGGAG 2 cut(s) 377, 612
Bpu14I TTCGAA 1 cut(s) 104
BpuEI CTTGAG 2 cut(s) 134, 1084
BpuMI CCSGG 1 cut(s) 991
Bsa29I ATCGAT 1 cut(s) 762
BsaAI YACGTR 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 1302
BsaWI WCCGGW 2 cut(s) 37, 740
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse118I RCCGGY 1 cut(s) 740
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 2 cut(s) 416, 450
BseCI ATCGAT 1 cut(s) 762
BseDI CCNNGG 1 cut(s) 1302
BseGI GGATG 2 cut(s) 319, 554
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMII CTCAG 3 cut(s) 51, 87, 159
BseRI GAGGAG 2 cut(s) 152, 1209
BseXI GCAGC 4 cut(s) 385, 556, 728, 775
BseYI CCCAGC 2 cut(s) 755, 902
BshFI GGCC 1 cut(s) 1053
BshTI ACCGGT 1 cut(s) 740
BshVI ATCGAT 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 3 cut(s) 38, 741, 990
BslFI GGGAC 1 cut(s) 1043
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 4 cut(s) 164, 470, 776, 1302
BsmBI CGTCTC 2 cut(s) 776, 1302
BsmFI GGGAC 1 cut(s) 1043
BsmI GAATGC 1 cut(s) 536
BsnI GGCC 1 cut(s) 1053
Bso31I GGTCTC 1 cut(s) 470
Bsp119I TTCGAA 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 4 cut(s) 238, 703, 759, 983
BspACI CCGC 2 cut(s) 54, 1147
BspANI GGCC 1 cut(s) 1053
BspCNI CTCAG 3 cut(s) 52, 88, 160
BspDI ATCGAT 1 cut(s) 762
BspLI GGNNCC 1 cut(s) 886
BspMAI CTGCAG 2 cut(s) 403, 1133
BspPI GGATC 4 cut(s) 233, 698, 767, 991
BspQI GCTCTTC 1 cut(s) 272
BspT104I TTCGAA 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 740
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 1 cut(s) 1302
BssMI GATC 4 cut(s) 238, 703, 759, 983
BssNAI GTATAC 1 cut(s) 738
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 1 cut(s) 1302
Bst1107I GTATAC 1 cut(s) 738
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 2 cut(s) 416, 450
Bst4CI ACNGT 5 cut(s) 493, 604, 671, 682, 1254
Bst6I CTCTTC 4 cut(s) 272, 336, 341, 945
BstBAI YACGTR 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 7, 935, 1051
BstDEI CTNAG 4 cut(s) 60, 96, 168, 712
BstF5I GGATG 2 cut(s) 319, 554
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 4 cut(s) 241, 706, 762, 986
BstMAI GTCTC 4 cut(s) 164, 470, 776, 1302
BstMBI GATC 4 cut(s) 238, 703, 759, 983
BstMWI GCNNNNNNNGC 2 cut(s) 32, 533
BstNI CCWGG 2 cut(s) 416, 450
BstNSI RCATGY 1 cut(s) 902
BstSCI CCNGG 3 cut(s) 414, 448, 989
BstSFI CTRYAG 3 cut(s) 399, 543, 1129
BstSNI TACGTA 1 cut(s) 735
BstV1I GCAGC 4 cut(s) 385, 556, 728, 775
BstV2I GAAGAC 3 cut(s) 512, 832, 1017
BstZ17I GTATAC 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 762
BsuI GTATCC 2 cut(s) 866, 1224
BsuRI GGCC 1 cut(s) 1053
BsuTUI ATCGAT 1 cut(s) 762
BtgZI GCGATG 1 cut(s) 395
BtrI CACGTC 1 cut(s) 1057
BtsCI GGATG 2 cut(s) 319, 554
Cac8I GCNNGC 3 cut(s) 7, 935, 1051
CfoI GCGC 1 cut(s) 59
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 2 cut(s) 884, 1144
ClaI ATCGAT 1 cut(s) 762
CseI GACGC 1 cut(s) 1055
Csp6I GTAC 2 cut(s) 605, 613
CspAI ACCGGT 1 cut(s) 740
CviAII CATG 3 cut(s) 805, 899, 1033
CviQI GTAC 2 cut(s) 605, 613
DdeI CTNAG 4 cut(s) 60, 96, 168, 712
DpnI GATC 4 cut(s) 240, 705, 761, 985
DpnII GATC 4 cut(s) 238, 703, 759, 983
EaeI YGGCCR 1 cut(s) 1051
Eam1104I CTCTTC 4 cut(s) 272, 336, 341, 945
EarI CTCTTC 4 cut(s) 272, 336, 341, 945
Ecl136II GAGCTC 1 cut(s) 265
Eco105I TACGTA 1 cut(s) 735
Eco130I CCWWGG 1 cut(s) 1302
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 470
Eco32I GATATC 2 cut(s) 507, 781
Eco47I GGWCC 2 cut(s) 884, 1144
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 3 cut(s) 309, 360, 593
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 414, 448
EcoRV GATATC 2 cut(s) 507, 781
EcoT14I CCWWGG 1 cut(s) 1302
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 1302
Esp3I CGTCTC 2 cut(s) 776, 1302
FaeI CATG 3 cut(s) 808, 902, 1036
FaqI GGGAC 1 cut(s) 1043
FatI CATG 3 cut(s) 804, 898, 1032
FblI GTMKAC 1 cut(s) 737
Fnu4HI GCNGC 5 cut(s) 55, 399, 570, 717, 789
FokI GGATG 2 cut(s) 326, 561
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 5 cut(s) 55, 399, 570, 717, 789
FspBI CTAG 3 cut(s) 482, 1016, 1074
GlaI GCGC 1 cut(s) 58
GluI GCNGC 5 cut(s) 55, 399, 570, 717, 789
GsaI CCCAGC 2 cut(s) 759, 906
GsuI CTGGAG 2 cut(s) 377, 612
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 1 cut(s) 1053
HapII CCGG 3 cut(s) 38, 741, 990
HgaI GACGC 1 cut(s) 1055
HhaI GCGC 1 cut(s) 59
Hin1II CATG 3 cut(s) 808, 902, 1036
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 6 cut(s) 62, 92, 809, 1117, 1183, 1193
HpaII CCGG 3 cut(s) 38, 741, 990
HphI GGTGA 2 cut(s) 305, 1166
Hpy166II GTNNAC 1 cut(s) 738
Hpy188I TCNGA 6 cut(s) 97, 340, 434, 511, 860, 894
Hpy188III TCNNGA 8 cut(s) 17, 134, 276, 287, 591, 725, 797, 1114
Hpy8I GTNNAC 1 cut(s) 738
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 6 cut(s) 149, 211, 255, 284, 697, 1135
HpyCH4III ACNGT 5 cut(s) 493, 604, 671, 682, 1254
HpyCH4IV ACGT 3 cut(s) 734, 1056, 1135
HpyCH4V TGCA 7 cut(s) 122, 401, 536, 674, 1094, 1131, 1287
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 533
HpyF3I CTNAG 4 cut(s) 60, 96, 168, 712
HpySE526I ACGT 3 cut(s) 734, 1056, 1135
Hsp92II CATG 3 cut(s) 808, 902, 1036
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 4 cut(s) 238, 703, 759, 983
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 2 cut(s) 40, 358
Lsp1109I GCAGC 4 cut(s) 385, 556, 728, 775
LweI GCATC 1 cut(s) 539
MaeI CTAG 3 cut(s) 482, 1016, 1074
MaeII ACGT 3 cut(s) 734, 1056, 1135
MaeIII GTNAC 2 cut(s) 872, 1190
MalI GATC 4 cut(s) 240, 705, 761, 985
MboI GATC 4 cut(s) 238, 703, 759, 983
MfeI CAATTG 1 cut(s) 86
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 1 cut(s) 1053
MluCI AATT 5 cut(s) 86, 249, 559, 629, 1108
MluNI TGGCCA 1 cut(s) 1053
MlyI GAGTC 4 cut(s) 71, 818, 1111, 1187
Mox20I TGGCCA 1 cut(s) 1053
MscI TGGCCA 1 cut(s) 1053
MseI TTAA 3 cut(s) 849, 1101, 1169
MslI CAYNNNNRTG 1 cut(s) 651
Msp20I TGGCCA 1 cut(s) 1053
MspI CCGG 3 cut(s) 38, 741, 990
MspR9I CCNGG 3 cut(s) 416, 450, 991
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 536
MvaI CCWGG 2 cut(s) 416, 450
MwoI GCNNNNNNNGC 2 cut(s) 32, 533
NciI CCSGG 1 cut(s) 991
NdeII GATC 4 cut(s) 238, 703, 759, 983
NlaIII CATG 3 cut(s) 808, 902, 1036
NlaIV GGNNCC 1 cut(s) 886
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 2 cut(s) 872, 1190
NspI RCATGY 1 cut(s) 902
NspV TTCGAA 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 272
PcsI WCGNNNNNNNCGW 2 cut(s) 418, 1053
PctI GAATGC 1 cut(s) 536
PfeI GAWTC 2 cut(s) 92, 1183
PflMI CCANNNNNTGG 1 cut(s) 590
PinAI ACCGGT 1 cut(s) 740
PkrI GCNGC 5 cut(s) 56, 400, 571, 718, 790
PleI GAGTC 4 cut(s) 70, 817, 1111, 1187
PpsI GAGTC 4 cut(s) 70, 817, 1111, 1187
Ppu21I YACGTR 1 cut(s) 735
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 2 cut(s) 414, 448
PspFI CCCAGC 2 cut(s) 755, 902
PspGI CCWGG 2 cut(s) 414, 448
PspN4I GGNNCC 1 cut(s) 886
PspPI GGNCC 2 cut(s) 884, 1144
PstI CTGCAG 2 cut(s) 403, 1133
RsaI GTAC 2 cut(s) 606, 614
RsaNI GTAC 2 cut(s) 605, 613
RseI CAYNNNNRTG 1 cut(s) 651
SacI GAGCTC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 3 cut(s) 849, 1101, 1169
SatI GCNGC 5 cut(s) 55, 399, 570, 717, 789
Sau3AI GATC 4 cut(s) 238, 703, 759, 983
Sau96I GGNCC 2 cut(s) 884, 1144
SchI GAGTC 4 cut(s) 71, 818, 1111, 1187
ScrFI CCNGG 3 cut(s) 416, 450, 991
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 1 cut(s) 539
SfcI CTRYAG 3 cut(s) 399, 543, 1129
SfuI TTCGAA 1 cut(s) 104
SinI GGWCC 2 cut(s) 884, 1144
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 2 cut(s) 113, 1063
SmoI CTYRAG 2 cut(s) 113, 1063
SnaBI TACGTA 1 cut(s) 735
Sse9I AATT 5 cut(s) 86, 249, 559, 629, 1108
SsiI CCGC 2 cut(s) 54, 1147
SspMI CTAG 3 cut(s) 482, 1016, 1074
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 3 cut(s) 414, 448, 989
StyI CCWWGG 1 cut(s) 1302
TaaI ACNGT 5 cut(s) 493, 604, 671, 682, 1254
TaiI ACGT 3 cut(s) 737, 1059, 1138
TaqI TCGA 5 cut(s) 104, 702, 762, 972, 1115
TasI AATT 5 cut(s) 86, 249, 559, 629, 1108
TatI WGTACW 1 cut(s) 604
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 2 cut(s) 92, 1183
Tru1I TTAA 3 cut(s) 849, 1101, 1169
Tru9I TTAA 3 cut(s) 849, 1101, 1169
TseFI GTSAC 2 cut(s) 872, 1190
TseI GCWGC 4 cut(s) 398, 569, 716, 788
Tsp45I GTSAC 2 cut(s) 872, 1190
TspDTI ATGAA 3 cut(s) 398, 641, 1049
TspGWI ACGGA 2 cut(s) 758, 846
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 2 cut(s) 884, 1144
XapI RAATTY 1 cut(s) 1108
XceI RCATGY 1 cut(s) 902
XcmI CCANNNNNNNNNTGG 1 cut(s) 1168
XmiI GTMKAC 1 cut(s) 737
XspI CTAG 3 cut(s) 482, 1016, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.