RH1AG090800

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0003170
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
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UTR
Exon/CDS
Intron
Rh1AG090800.1

Sequence Viewer

Length: 1392 bp
ATGACATCTTCTTCATCAAGTATGGCAGGGGAGAACTTGGATGAAAATAATCAAAATATTCCTTGCGAGGAAAATGGGACTGTAGAATCTTCGAATGGCAATGGTAATGGAGAAGCCGCTCAACAATCTCCAACTGTTCAGAAAGATGAGGATGAAGACAAAGATGTTTCAAAAAAGAAGAAGAAGAAAAATAAGAGCAAGAAAAAGAAAGCACATGCTGAGCATACTGATCCACAATCTTTAAGTGGGCAAACTGATCCACAATCTACAACTAGGCAAACTGCTCCACAATCTACAAGTAGGCAAACTGATCCACCATCCATTCCTCTTATTGATCTGTTCCCACATGGGGAGTTTCCTGAGGGCGAAATTCAACAATACAGAGATGACAACTTGTGGAGGACTACTTCTGAAGAGAAGAGGGAGTTGGAGCGCCTTGAAAAACCATTATATAATTCAGTTCGTCGAGCTGCAGAAGTTCATCGCCAGGTACGGAAATACATCAGAGGGATTTTAAAGCCTGGAATGTTGATGACTGATTTGTGTGAGACTTTGGAGAACACGGTCCGTAAGTTAATATCCGAGAATGGTCTGGAAGCAGGCATTGCATTCCCCACAGGATGCTCTTTGAATTGGGTTGCTGCTCATTGGACCCCAAATACAGGGGACAAGACGGTGCTTCAGTATGATGATGTGATGAAATTAGACTTTGGAACTCATGTTGATGGTCATATAGTTGACTGTGCATTTACAGTAGCATTCAATCCTATGTTTGATCCACTACTTGAAGCCTCACGTGAAGCAACAAATACGGGTATCAAGGAGTCTGGAATTGATGTGCGTCTTTGTGATGTTGGTGCTGCAATCCAAGAAGTCATGGAATCATATGAGGTTGAAATAAATGGGAAGGTGTATCAAGTTAAAAGTATCCGGAACTTGAATGGACATAGCATTGGACCGTACCAAATCCATGCTGGAAAATCAGTTCCCATTGTGAAAGGAGGGGAGCAGACAAAAATGGAAGAGGGTGAATTTTTTGCGATTGAAACTTTCGCATCAACTGGGAAAGGATATGTCAGAGAAGATCTAGAGTGCAGCCATTACATGAAAAATTTTGATGCCGGCCACATCCCACTGAGGCTGCCTAGGGCAAAGCAATTGCTAGCAACAATTAACAAGAACTTCTCCACATTGGCGTTCTGCAGGCGATATTTAGACCGCCTGGGGGAGACCAAGTATCTTATGGCGCTTAAGAATTTGTGCGATGCTGGCATTGTCCAGCCCTATCCTCCTCTCTGTGATGTTAAAGGGAGCTATGTATCTCAGTTTGAGCATACAATTTTACTCCGGCCAACCTGCAAAGAGGTCATATCAAGAGGCGATGATTACTGA

Protein Analysis

463

Amino Acids

51.74

Weight (kDa)

5.74

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 154 - 357 1.8e-27 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1364
AccBSI CCGCTC 1 cut(s) 119
AccIII TCCGGA 1 cut(s) 930
AciI CCGC 2 cut(s) 117, 1219
AclWI GGATC 4 cut(s) 224, 251, 305, 770
AcoI YGGCCR 2 cut(s) 1123, 1349
AcsI RAATTY 4 cut(s) 369, 1031, 1111, 1255
AcuI CTGAAG 2 cut(s) 432, 665
AcvI CACGTG 1 cut(s) 797
AfaI GTAC 2 cut(s) 492, 962
AfiI CCNNNNNNNGG 3 cut(s) 349, 662, 1225
AflII CTTAAG 1 cut(s) 1250
AgsI TTSAA 9 cut(s) 171, 374, 440, 631, 763, 788, 896, 940, 1046
AjnI CCWGG 3 cut(s) 486, 520, 1221
AjuI GAANNNNNNNTTGG 2 cut(s) 410, 442
AloI GAACNNNNNNTCC 2 cut(s) 969, 1001
AluBI AGCT 2 cut(s) 470, 1314
AluI AGCT 2 cut(s) 470, 1314
Alw26I GTCTC 2 cut(s) 542, 1223
AlwI GGATC 4 cut(s) 224, 251, 305, 770
Aor13HI TCCGGA 1 cut(s) 930
AoxI GGCC 2 cut(s) 1123, 1349
ApeKI GCWGC 5 cut(s) 470, 641, 860, 1095, 1141
ApoI RAATTY 4 cut(s) 369, 1031, 1111, 1255
AspA2I CCTAGG 1 cut(s) 1145
AspLEI GCGC 2 cut(s) 435, 1249
AspS9I GGNCC 3 cut(s) 565, 651, 956
AsuHPI GGTGA 1 cut(s) 1040
AsuII TTCGAA 1 cut(s) 92
AsuNHI GCTAGC 1 cut(s) 1162
AvaII GGWCC 3 cut(s) 565, 651, 956
AvrII CCTAGG 1 cut(s) 1145
AxyI CCTNAGG 1 cut(s) 360
BbrPI CACGTG 1 cut(s) 797
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 5 cut(s) 457, 628, 847, 1107, 1128
BccI CCATC 2 cut(s) 325, 719
BciT130I CCWGG 3 cut(s) 488, 522, 1223
BciVI GTATCC 1 cut(s) 938
BcoDI GTCTC 2 cut(s) 542, 1223
BfaI CTAG 4 cut(s) 273, 1088, 1146, 1163
BfmI CTRYAG 3 cut(s) 81, 471, 1201
BfoI RGCGCY 2 cut(s) 436, 1250
BfrI CTTAAG 1 cut(s) 1250
BfuAI ACCTGC 1 cut(s) 1364
BfuI GTATCC 1 cut(s) 938
BglII AGATCT 1 cut(s) 1084
BisI GCNGC 6 cut(s) 117, 471, 642, 861, 1096, 1142
BlnI CCTAGG 1 cut(s) 1145
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 6 cut(s) 118, 472, 643, 862, 1097, 1143
Bme1390I CCNGG 3 cut(s) 488, 522, 1223
Bme18I GGWCC 3 cut(s) 565, 651, 956
BmgT120I GGNCC 3 cut(s) 565, 651, 956
BmiI GGNNCC 1 cut(s) 653
BmrFI CCNGG 3 cut(s) 488, 522, 1223
BmrI ACTGGG 1 cut(s) 1071
BmsI GCATC 4 cut(s) 611, 1064, 1108, 1255
BmtI GCTAGC 1 cut(s) 1166
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 797
BsaI GGTCTC 1 cut(s) 1223
BsaJI CCNNGG 2 cut(s) 1145, 1222
BsaWI WCCGGW 1 cut(s) 930
BsaXI ACNNNNNCTCC 2 cut(s) 1220, 1250
Bsc4I CCNNNNNNNGG 3 cut(s) 349, 662, 1225
Bse118I RCCGGY 1 cut(s) 1121
Bse1I ACTGG 1 cut(s) 1066
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 2 cut(s) 106, 603
BseAI TCCGGA 1 cut(s) 930
BseBI CCWGG 3 cut(s) 488, 522, 1223
BseDI CCNNGG 2 cut(s) 1145, 1222
BseGI GGATG 5 cut(s) 46, 157, 317, 626, 1128
BseLI CCNNNNNNNGG 3 cut(s) 349, 662, 1225
BseMI GCAATG 2 cut(s) 106, 603
BseMII CTCAG 4 cut(s) 210, 351, 1127, 1337
BseNI ACTGG 1 cut(s) 1066
BseRI GAGGAG 1 cut(s) 1281
BseXI GCAGC 5 cut(s) 457, 628, 847, 1107, 1128
BsgI GTGCAG 1 cut(s) 1114
BshFI GGCC 2 cut(s) 1125, 1351
BsiSI CCGG 3 cut(s) 931, 1122, 1348
BslFI GGGAC 2 cut(s) 91, 680
BslI CCNNNNNNNGG 3 cut(s) 349, 662, 1225
BsmAI GTCTC 2 cut(s) 542, 1223
BsmFI GGGAC 2 cut(s) 91, 680
BsmI GAATGC 2 cut(s) 608, 758
BsnI GGCC 2 cut(s) 1125, 1351
Bso31I GGTCTC 1 cut(s) 1223
Bsp119I TTCGAA 1 cut(s) 92
Bsp13I TCCGGA 1 cut(s) 930
Bsp143I GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 2 cut(s) 117, 1219
BspANI GGCC 2 cut(s) 1125, 1351
BspCNI CTCAG 4 cut(s) 211, 352, 1128, 1336
BspEI TCCGGA 1 cut(s) 930
BspLI GGNNCC 1 cut(s) 653
BspMAI CTGCAG 2 cut(s) 475, 1205
BspMI ACCTGC 1 cut(s) 1364
BspOI GCTAGC 1 cut(s) 1166
BspPI GGATC 4 cut(s) 224, 251, 305, 770
BspT104I TTCGAA 1 cut(s) 92
BspTI CTTAAG 1 cut(s) 1250
BspTNI GGTCTC 1 cut(s) 1223
BsrBI CCGCTC 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 106, 603
BsrFI RCCGGY 1 cut(s) 1121
BsrI ACTGG 1 cut(s) 1066
BssAI RCCGGY 1 cut(s) 1121
BssECI CCNNGG 2 cut(s) 1145, 1222
BssMI GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
BssT1I CCWWGG 1 cut(s) 1145
Bst2UI CCWGG 3 cut(s) 488, 522, 1223
Bst4CI ACNGT 7 cut(s) 82, 136, 565, 676, 743, 754, 960
Bst6I CTCTTC 3 cut(s) 408, 413, 1017
BstAFI CTTAAG 1 cut(s) 1250
BstAPI GCANNNNNTGC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 797
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 5 cut(s) 601, 1123, 1164, 1205, 1270
BstDEI CTNAG 4 cut(s) 219, 360, 1136, 1323
BstF5I GGATG 5 cut(s) 46, 157, 317, 626, 1128
BstH2I RGCGCY 2 cut(s) 436, 1250
BstHHI GCGC 2 cut(s) 435, 1249
BstKTI GATC 6 cut(s) 232, 259, 313, 337, 778, 1087
BstMAI GTCTC 2 cut(s) 542, 1223
BstMBI GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
BstMWI GCNNNNNNNGC 2 cut(s) 605, 1269
BstNI CCWGG 3 cut(s) 488, 522, 1223
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 3 cut(s) 486, 520, 1221
BstSFI CTRYAG 3 cut(s) 81, 471, 1201
BstV1I GCAGC 5 cut(s) 457, 628, 847, 1107, 1128
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
Bsu36I CCTNAGG 1 cut(s) 360
BsuI GTATCC 1 cut(s) 938
BsuRI GGCC 2 cut(s) 1125, 1351
BtgZI GCGATG 2 cut(s) 467, 1278
BtsCI GGATG 5 cut(s) 46, 157, 317, 626, 1128
BtsIMutI CAGTG 1 cut(s) 1133
BveI ACCTGC 1 cut(s) 1364
Cac8I GCNNGC 5 cut(s) 601, 1123, 1164, 1205, 1270
CfoI GCGC 2 cut(s) 435, 1249
Cfr10I RCCGGY 1 cut(s) 1121
Cfr13I GGNCC 3 cut(s) 565, 651, 956
CpoI CGGWCCG 1 cut(s) 565
CseI GACGC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 491, 961
CspI CGGWCCG 1 cut(s) 565
CviAII CATG 6 cut(s) 215, 347, 719, 877, 971, 1105
CviQI GTAC 2 cut(s) 491, 961
DdeI CTNAG 4 cut(s) 219, 360, 1136, 1323
DpnI GATC 6 cut(s) 231, 258, 312, 336, 777, 1086
DpnII GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 2 cut(s) 1123, 1349
Eam1104I CTCTTC 3 cut(s) 408, 413, 1017
EarI CTCTTC 3 cut(s) 408, 413, 1017
Eco130I CCWWGG 1 cut(s) 1145
Eco31I GGTCTC 1 cut(s) 1223
Eco47I GGWCC 3 cut(s) 565, 651, 956
Eco57I CTGAAG 2 cut(s) 432, 665
Eco72I CACGTG 1 cut(s) 797
Eco81I CCTNAGG 1 cut(s) 360
EcoRII CCWGG 3 cut(s) 486, 520, 1221
EcoT14I CCWWGG 1 cut(s) 1145
ErhI CCWWGG 1 cut(s) 1145
FaeI CATG 6 cut(s) 218, 350, 722, 880, 974, 1108
FaqI GGGAC 2 cut(s) 91, 680
FatI CATG 6 cut(s) 214, 346, 718, 876, 970, 1104
FauNDI CATATG 1 cut(s) 886
Fnu4HI GCNGC 6 cut(s) 117, 471, 642, 861, 1096, 1142
FokI GGATG 5 cut(s) 53, 164, 304, 633, 1115
Fsp4HI GCNGC 6 cut(s) 117, 471, 642, 861, 1096, 1142
FspBI CTAG 4 cut(s) 273, 1088, 1146, 1163
GlaI GCGC 2 cut(s) 434, 1248
GluI GCNGC 6 cut(s) 117, 471, 642, 861, 1096, 1142
HaeII RGCGCY 2 cut(s) 436, 1250
HaeIII GGCC 2 cut(s) 1125, 1351
HapII CCGG 3 cut(s) 931, 1122, 1348
HgaI GACGC 1 cut(s) 830
HhaI GCGC 2 cut(s) 435, 1249
Hin1II CATG 6 cut(s) 218, 350, 722, 880, 974, 1108
Hin6I GCGC 2 cut(s) 433, 1247
HinP1I GCGC 2 cut(s) 433, 1247
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HinfI GANTC 3 cut(s) 86, 824, 881
HpaII CCGG 3 cut(s) 931, 1122, 1348
HphI GGTGA 1 cut(s) 1040
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 5 cut(s) 141, 412, 506, 583, 1079
Hpy188III TCNNGA 6 cut(s) 359, 593, 828, 931, 1088, 1374
Hpy8I GTNNAC 1 cut(s) 739
Hpy99I CGWCG 1 cut(s) 468
HpyAV CCTTC 1 cut(s) 901
HpyCH4III ACNGT 7 cut(s) 82, 136, 565, 676, 743, 754, 960
HpyCH4IV ACGT 1 cut(s) 796
HpyCH4V TGCA 7 cut(s) 473, 608, 746, 863, 1095, 1203, 1359
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 1269
HpyF3I CTNAG 4 cut(s) 219, 360, 1136, 1323
HpySE526I ACGT 1 cut(s) 796
Hsp92II CATG 6 cut(s) 218, 350, 722, 880, 974, 1108
HspAI GCGC 2 cut(s) 433, 1247
Kpn2I TCCGGA 1 cut(s) 930
KroI GCCGGC 1 cut(s) 1121
KroNI GCCGGC 1 cut(s) 1123
Kzo9I GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
LmnI GCTCC 4 cut(s) 289, 430, 1006, 1311
Lsp1109I GCAGC 5 cut(s) 457, 628, 847, 1107, 1128
LweI GCATC 4 cut(s) 611, 1064, 1108, 1255
MaeI CTAG 4 cut(s) 273, 1088, 1146, 1163
MaeII ACGT 1 cut(s) 796
MalI GATC 6 cut(s) 231, 258, 312, 336, 777, 1086
MbiI CCGCTC 1 cut(s) 119
MboI GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
MfeI CAATTG 1 cut(s) 1157
MflI RGATCY 1 cut(s) 1084
MlyI GAGTC 1 cut(s) 833
MmeI TCCRAC 2 cut(s) 155, 408
MroI TCCGGA 1 cut(s) 930
MroNI GCCGGC 1 cut(s) 1121
MseI TTAA 7 cut(s) 242, 515, 575, 921, 1173, 1251, 1305
MslI CAYNNNNRTG 1 cut(s) 723
MspCI CTTAAG 1 cut(s) 1250
MspI CCGG 3 cut(s) 931, 1122, 1348
MspR9I CCNGG 3 cut(s) 488, 522, 1223
MunI CAATTG 1 cut(s) 1157
Mva1269I GAATGC 2 cut(s) 608, 758
MvaI CCWGG 3 cut(s) 488, 522, 1223
MwoI GCNNNNNNNGC 2 cut(s) 605, 1269
NaeI GCCGGC 1 cut(s) 1123
NdeI CATATG 1 cut(s) 886
NdeII GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
NgoMIV GCCGGC 1 cut(s) 1121
NheI GCTAGC 1 cut(s) 1162
NlaIII CATG 6 cut(s) 218, 350, 722, 880, 974, 1108
NlaIV GGNNCC 1 cut(s) 653
NspI RCATGY 1 cut(s) 218
NspV TTCGAA 1 cut(s) 92
PctI GAATGC 2 cut(s) 608, 758
PdiI GCCGGC 1 cut(s) 1123
PfeI GAWTC 2 cut(s) 86, 881
PkrI GCNGC 6 cut(s) 118, 472, 643, 862, 1097, 1143
PleI GAGTC 1 cut(s) 832
PmaCI CACGTG 1 cut(s) 797
PmlI CACGTG 1 cut(s) 797
PpsI GAGTC 1 cut(s) 832
Ppu21I YACGTR 1 cut(s) 797
Psp6I CCWGG 3 cut(s) 486, 520, 1221
PspCI CACGTG 1 cut(s) 797
PspGI CCWGG 3 cut(s) 486, 520, 1221
PspN4I GGNNCC 1 cut(s) 653
PspPI GGNCC 3 cut(s) 565, 651, 956
PstI CTGCAG 2 cut(s) 475, 1205
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 2 cut(s) 492, 962
RsaNI GTAC 2 cut(s) 491, 961
RseI CAYNNNNRTG 1 cut(s) 723
Rsr2I CGGWCCG 1 cut(s) 565
RsrII CGGWCCG 1 cut(s) 565
SaqAI TTAA 7 cut(s) 242, 515, 575, 921, 1173, 1251, 1305
SatI GCNGC 6 cut(s) 117, 471, 642, 861, 1096, 1142
Sau3AI GATC 6 cut(s) 229, 256, 310, 334, 775, 1084
Sau96I GGNCC 3 cut(s) 565, 651, 956
SchI GAGTC 1 cut(s) 833
ScrFI CCNGG 3 cut(s) 488, 522, 1223
SetI ASST 8 cut(s) 472, 492, 799, 894, 912, 1316, 1358, 1368
SfaNI GCATC 4 cut(s) 611, 1064, 1108, 1255
SfcI CTRYAG 3 cut(s) 81, 471, 1201
SfuI TTCGAA 1 cut(s) 92
SinI GGWCC 3 cut(s) 565, 651, 956
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 1 cut(s) 1250
SmoI CTYRAG 1 cut(s) 1250
SsiI CCGC 2 cut(s) 117, 1219
SspI AATATT 1 cut(s) 58
SspMI CTAG 4 cut(s) 273, 1088, 1146, 1163
StyD4I CCNGG 3 cut(s) 486, 520, 1221
StyI CCWWGG 1 cut(s) 1145
TaaI ACNGT 7 cut(s) 82, 136, 565, 676, 743, 754, 960
TaiI ACGT 1 cut(s) 799
TaqI TCGA 2 cut(s) 92, 466
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 2 cut(s) 86, 881
Tru1I TTAA 7 cut(s) 242, 515, 575, 921, 1173, 1251, 1305
Tru9I TTAA 7 cut(s) 242, 515, 575, 921, 1173, 1251, 1305
TscAI CASTG 1 cut(s) 1140
TseI GCWGC 5 cut(s) 470, 641, 860, 1095, 1141
TspDTI ATGAA 5 cut(s) 57, 168, 470, 713, 1121
TspGWI ACGGA 2 cut(s) 508, 557
TspRI CASTG 1 cut(s) 1140
Vha464I CTTAAG 1 cut(s) 1250
VpaK11BI GGWCC 3 cut(s) 565, 651, 956
XapI RAATTY 4 cut(s) 369, 1031, 1111, 1255
XbaI TCTAGA 1 cut(s) 1087
XceI RCATGY 1 cut(s) 218
XcmI CCANNNNNNNNNTGG 2 cut(s) 971, 1240
XmaJI CCTAGG 1 cut(s) 1145
XspI CTAG 4 cut(s) 273, 1088, 1146, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.