MD17G1285400.v1.1

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003170
Physical Location & Seq
Reverse (-)
34448027 .. 34455605
7579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1285400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGCAAGGGCAGACTGATCCACCATCTATTCCTCTTGTTGACCTGTTCCCATCTGGGGAGTTTCCCGAGGGTGAAATTCAACAGTACAAAGACGATAACCTTTGGAGGACTACCTCTGAAGAGAAGAGGGAGCTGGAGCGCCTTGAAAAACCAATGTATAATTCTGTTCGTCGAGCGGCAGAAGTTCATCGCCAGGTTCGGAAATACATCAAAGGCATCTTAAGGCCTGGAATGTTGATGACTGATTTGTGTGAGACTCTGGAGAACACAGTGAGGAAGTTGATATCTGAGAATGGTCTGGATGCAGGCATTGCTTTCCCGACAGGATGCTCTTTGAATTGGGTTGCTGCTCATTGGACCCCTAATACAGGGGACAAGACTGTGCTTCAGTATGATGATGTAATGAAGCTGGATTTTGGAACTCATGTTGACGGAAACATAGTTGACTGCGCTTTTACAGTAGCATTTAATCCTATGTTTGATCCACTCCTTGAAGCCTCGCGTGAAGCAACAAATACGGGTATCAAGGAGTCTGGAATTGATGTGCGTCTTTGTGATGTTGGTGCTGCAATACAAGAAGTCATGGAATCATATGAGGTTGAAATAAATGGAAAGGTTTATCAAGTTAAAAGTATTCGAAACTTGAATGGACATAGCATTGGACGTTATCAAATCCATGCTGGAAAATCTGTTCCTATTGTGAAAGGCGGGGAGCAGACGAAAATGGAAGAGGGTGAATTTTTCGCAATTGAAACTTTTGCATCAACTGGGAAAGGGTATGTCAGAGAAGATCTAGAGTGCAGCCATTACATGAAAAATTTTGAAGCTGGCCACATCCCACTGAGGTTGCCCAGGGCAAAGCAATTGCTAGCAACAATTAACAAGAACTTCTCCACATTGGCCTTCTGCAGGCGTTACTTAGACCGCCTTGGAGAGACTAAGTATCTTATGGCACTAAAGAATCTGTGTGATGCCGGCATTGTGCAGCCCTATCCTCCTCTCTGTGATGTTAAGGGCAGCTATGTTTCTCAGTTTGAGCATACCATTTTACTCCGGCCAACCTGCAAAGAGGTCATATCCAGAGGCGACGACTACTGA

Protein Analysis

366

Amino Acids

41.15

Weight (kDa)

5.62

Isoelectric Point (pI)

30.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 56 - 259 1.6e-27 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1070
AccBSI CCGCTC 1 cut(s) 176
AccII CGCG 1 cut(s) 502
AciI CCGC 3 cut(s) 176, 708, 925
AclWI GGATC 2 cut(s) 11, 476
AcoI YGGCCR 2 cut(s) 829, 1055
AcsI RAATTY 3 cut(s) 75, 737, 817
AcuI CTGAAG 2 cut(s) 138, 371
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 3 cut(s) 55, 368, 909
AflII CTTAAG 1 cut(s) 220
AgsI TTSAA 8 cut(s) 80, 146, 337, 494, 602, 646, 752, 824
AjnI CCWGG 3 cut(s) 192, 226, 851
AjuI GAANNNNNNNTTGG 2 cut(s) 145, 177
AloI GAACNNNNNNTCC 2 cut(s) 675, 707
AluBI AGCT 4 cut(s) 133, 409, 827, 1020
AluI AGCT 4 cut(s) 133, 409, 827, 1020
Alw26I GTCTC 2 cut(s) 248, 929
AlwI GGATC 2 cut(s) 11, 476
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 4 cut(s) 224, 829, 900, 1055
ApeKI GCWGC 5 cut(s) 347, 566, 801, 985, 1017
ApoI RAATTY 3 cut(s) 75, 737, 817
AspLEI GCGC 2 cut(s) 141, 452
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 2 cut(s) 83, 746
AsuII TTCGAA 1 cut(s) 637
AsuNHI GCTAGC 1 cut(s) 868
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 357
BalI TGGCCA 1 cut(s) 831
BbvI GCAGC 5 cut(s) 334, 553, 813, 997, 1029
BccI CCATC 2 cut(s) 31, 58
BciT130I CCWGG 3 cut(s) 194, 228, 853
BcoDI GTCTC 2 cut(s) 248, 929
BfaI CTAG 2 cut(s) 794, 869
BfmI CTRYAG 1 cut(s) 907
BfoI RGCGCY 1 cut(s) 142
BfrI CTTAAG 1 cut(s) 220
BfuAI ACCTGC 1 cut(s) 1070
BglII AGATCT 1 cut(s) 790
BisI GCNGC 6 cut(s) 177, 348, 567, 802, 986, 1018
BlsI GCNGC 6 cut(s) 178, 349, 568, 803, 987, 1019
Bme1390I CCNGG 3 cut(s) 194, 228, 853
Bme18I GGWCC 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 3 cut(s) 194, 228, 853
BmrI ACTGGG 1 cut(s) 777
BmsI GCATC 5 cut(s) 225, 292, 317, 770, 961
BmtI GCTAGC 1 cut(s) 872
BmuI ACTGGG 1 cut(s) 777
BpmI CTGGAG 2 cut(s) 155, 281
Bpu14I TTCGAA 1 cut(s) 637
BsaJI CCNNGG 4 cut(s) 66, 851, 852, 928
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 368, 909
Bse118I RCCGGY 1 cut(s) 974
Bse1I ACTGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 3 cut(s) 194, 228, 853
BseDI CCNNGG 4 cut(s) 66, 851, 852, 928
BseGI GGATG 3 cut(s) 307, 332, 834
BseLI CCNNNNNNNGG 3 cut(s) 55, 368, 909
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 279, 833, 1043
BseNI ACTGG 1 cut(s) 772
BseRI GAGGAG 1 cut(s) 987
BseXI GCAGC 5 cut(s) 334, 553, 813, 997, 1029
BsgI GTGCAG 2 cut(s) 820, 1004
Bsh1236I CGCG 1 cut(s) 502
BshFI GGCC 4 cut(s) 226, 831, 902, 1057
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 975, 1054
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 3 cut(s) 55, 368, 909
BsmAI GTCTC 2 cut(s) 248, 929
BsmFI GGGAC 1 cut(s) 386
BsnI GGCC 4 cut(s) 226, 831, 902, 1057
BsoBI CYCGRG 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 637
Bsp143I GATC 3 cut(s) 16, 481, 790
BspACI CCGC 3 cut(s) 176, 708, 925
BspANI GGCC 4 cut(s) 226, 831, 902, 1057
BspCNI CTCAG 3 cut(s) 280, 834, 1042
BspFNI CGCG 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 359
BspMAI CTGCAG 1 cut(s) 911
BspMI ACCTGC 1 cut(s) 1070
BspOI GCTAGC 1 cut(s) 872
BspPI GGATC 2 cut(s) 11, 476
BspT104I TTCGAA 1 cut(s) 637
BspTI CTTAAG 1 cut(s) 220
BsrBI CCGCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 974
BsrI ACTGG 1 cut(s) 772
BssAI RCCGGY 1 cut(s) 974
BssECI CCNNGG 4 cut(s) 66, 851, 852, 928
BssMI GATC 3 cut(s) 16, 481, 790
BssT1I CCWWGG 1 cut(s) 928
Bst2UI CCWGG 3 cut(s) 194, 228, 853
Bst4CI ACNGT 4 cut(s) 84, 271, 382, 460
Bst6I CTCTTC 3 cut(s) 114, 119, 723
BstAFI CTTAAG 1 cut(s) 220
BstAPI GCANNNNNTGC 1 cut(s) 311
BstBI TTCGAA 1 cut(s) 637
BstC8I GCNNGC 5 cut(s) 307, 829, 870, 911, 976
BstDEI CTNAG 5 cut(s) 288, 842, 919, 939, 1029
BstENI CCTNNNNNAGG 2 cut(s) 366, 907
BstF5I GGATG 3 cut(s) 307, 332, 834
BstFNI CGCG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 2 cut(s) 141, 452
BstKTI GATC 3 cut(s) 19, 484, 793
BstMAI GTCTC 2 cut(s) 248, 929
BstMBI GATC 3 cut(s) 16, 481, 790
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 3 cut(s) 194, 228, 853
BstSCI CCNGG 3 cut(s) 192, 226, 851
BstSFI CTRYAG 1 cut(s) 907
BstUI CGCG 1 cut(s) 502
BstV1I GCAGC 5 cut(s) 334, 553, 813, 997, 1029
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BsuRI GGCC 4 cut(s) 226, 831, 902, 1057
BtgZI GCGATG 1 cut(s) 173
BtsCI GGATG 3 cut(s) 307, 332, 834
BtsIMutI CAGTG 2 cut(s) 276, 839
BveI ACCTGC 1 cut(s) 1070
Cac8I GCNNGC 5 cut(s) 307, 829, 870, 911, 976
CfoI GCGC 2 cut(s) 141, 452
Cfr10I RCCGGY 1 cut(s) 974
Cfr13I GGNCC 1 cut(s) 357
CseI GACGC 1 cut(s) 536
Csp6I GTAC 1 cut(s) 85
CspCI CAANNNNNGTGG 2 cut(s) 828, 863
CviAII CATG 4 cut(s) 425, 583, 677, 811
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 5 cut(s) 288, 842, 919, 939, 1029
DpnI GATC 3 cut(s) 18, 483, 792
DpnII GATC 3 cut(s) 16, 481, 790
EaeI YGGCCR 2 cut(s) 829, 1055
Eam1104I CTCTTC 3 cut(s) 114, 119, 723
EarI CTCTTC 3 cut(s) 114, 119, 723
Eco130I CCWWGG 1 cut(s) 928
Eco147I AGGCCT 1 cut(s) 226
Eco32I GATATC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 138, 371
Eco88I CYCGRG 1 cut(s) 65
EcoNI CCTNNNNNAGG 2 cut(s) 366, 907
EcoRII CCWGG 3 cut(s) 192, 226, 851
EcoRV GATATC 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 928
ErhI CCWWGG 1 cut(s) 928
FaeI CATG 4 cut(s) 428, 586, 680, 814
FaqI GGGAC 1 cut(s) 386
FatI CATG 4 cut(s) 424, 582, 676, 810
FauI CCCGC 1 cut(s) 701
FauNDI CATATG 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 177, 348, 567, 802, 986, 1018
FokI GGATG 3 cut(s) 314, 339, 821
Fsp4HI GCNGC 6 cut(s) 177, 348, 567, 802, 986, 1018
FspBI CTAG 2 cut(s) 794, 869
GlaI GCGC 2 cut(s) 140, 451
GluI GCNGC 6 cut(s) 177, 348, 567, 802, 986, 1018
GsuI CTGGAG 2 cut(s) 155, 281
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 226, 831, 902, 1057
HapII CCGG 2 cut(s) 975, 1054
HgaI GACGC 1 cut(s) 536
HhaI GCGC 2 cut(s) 141, 452
Hin1II CATG 4 cut(s) 428, 586, 680, 814
Hin6I GCGC 2 cut(s) 139, 450
HinP1I GCGC 2 cut(s) 139, 450
HincII GTYRAC 3 cut(s) 40, 430, 445
HindII GTYRAC 3 cut(s) 40, 430, 445
HinfI GANTC 4 cut(s) 256, 530, 587, 961
HpaII CCGG 2 cut(s) 975, 1054
HphI GGTGA 2 cut(s) 83, 746
Hpy166II GTNNAC 3 cut(s) 40, 430, 445
Hpy188I TCNGA 4 cut(s) 118, 201, 289, 785
Hpy188III TCNNGA 7 cut(s) 65, 260, 299, 319, 534, 794, 1080
Hpy8I GTNNAC 3 cut(s) 40, 430, 445
Hpy99I CGWCG 2 cut(s) 174, 1091
HpyAV CCTTC 1 cut(s) 913
HpyCH4III ACNGT 4 cut(s) 84, 271, 382, 460
HpyCH4IV ACGT 1 cut(s) 664
HpyCH4V TGCA 8 cut(s) 4, 305, 569, 761, 801, 909, 985, 1065
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 5 cut(s) 288, 842, 919, 939, 1029
HpySE526I ACGT 1 cut(s) 664
Hsp92II CATG 4 cut(s) 428, 586, 680, 814
HspAI GCGC 2 cut(s) 139, 450
KroI GCCGGC 1 cut(s) 974
KroNI GCCGGC 1 cut(s) 976
Kzo9I GATC 3 cut(s) 16, 481, 790
LmnI GCTCC 3 cut(s) 130, 136, 712
Lsp1109I GCAGC 5 cut(s) 334, 553, 813, 997, 1029
LweI GCATC 5 cut(s) 225, 292, 317, 770, 961
MaeI CTAG 2 cut(s) 794, 869
MaeII ACGT 1 cut(s) 664
MaeIII GTNAC 1 cut(s) 914
MalI GATC 3 cut(s) 18, 483, 792
MbiI CCGCTC 1 cut(s) 176
MboI GATC 3 cut(s) 16, 481, 790
MboII GAAGA 4 cut(s) 131, 136, 740, 800
MfeI CAATTG 2 cut(s) 747, 863
MflI RGATCY 1 cut(s) 790
MlsI TGGCCA 1 cut(s) 831
MluCI AATT 9 cut(s) 75, 160, 337, 537, 737, 747, 817, 863, 876
MluNI TGGCCA 1 cut(s) 831
MlyI GAGTC 2 cut(s) 250, 539
Mox20I TGGCCA 1 cut(s) 831
MroNI GCCGGC 1 cut(s) 974
MscI TGGCCA 1 cut(s) 831
MseI TTAA 5 cut(s) 221, 468, 627, 879, 1011
Msp20I TGGCCA 1 cut(s) 831
MspCI CTTAAG 1 cut(s) 220
MspI CCGG 2 cut(s) 975, 1054
MspR9I CCNGG 3 cut(s) 194, 228, 853
MunI CAATTG 2 cut(s) 747, 863
MvaI CCWGG 3 cut(s) 194, 228, 853
MvnI CGCG 1 cut(s) 502
MwoI GCNNNNNNNGC 1 cut(s) 311
NaeI GCCGGC 1 cut(s) 976
NdeI CATATG 1 cut(s) 592
NdeII GATC 3 cut(s) 16, 481, 790
NgoMIV GCCGGC 1 cut(s) 974
NheI GCTAGC 1 cut(s) 868
NlaIII CATG 4 cut(s) 428, 586, 680, 814
NlaIV GGNNCC 1 cut(s) 359
NspV TTCGAA 1 cut(s) 637
PasI CCCWGGG 1 cut(s) 852
PceI AGGCCT 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 976
PfeI GAWTC 2 cut(s) 587, 961
PkrI GCNGC 6 cut(s) 178, 349, 568, 803, 987, 1019
PleI GAGTC 2 cut(s) 250, 538
PpsI GAGTC 2 cut(s) 250, 538
Psp6I CCWGG 3 cut(s) 192, 226, 851
PspGI CCWGG 3 cut(s) 192, 226, 851
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 911
PsuI RGATCY 1 cut(s) 790
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SaqAI TTAA 5 cut(s) 221, 468, 627, 879, 1011
SatI GCNGC 6 cut(s) 177, 348, 567, 802, 986, 1018
Sau3AI GATC 3 cut(s) 16, 481, 790
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 2 cut(s) 250, 539
ScrFI CCNGG 3 cut(s) 194, 228, 853
SfaNI GCATC 5 cut(s) 225, 292, 317, 770, 961
SfcI CTRYAG 1 cut(s) 907
SfuI TTCGAA 1 cut(s) 637
SinI GGWCC 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 9 cut(s) 75, 160, 337, 537, 737, 747, 817, 863, 876
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 3 cut(s) 176, 708, 925
SspMI CTAG 2 cut(s) 794, 869
StuI AGGCCT 1 cut(s) 226
StyD4I CCNGG 3 cut(s) 192, 226, 851
StyI CCWWGG 1 cut(s) 928
TaaI ACNGT 4 cut(s) 84, 271, 382, 460
TaiI ACGT 1 cut(s) 667
TaqI TCGA 2 cut(s) 172, 637
TasI AATT 9 cut(s) 75, 160, 337, 537, 737, 747, 817, 863, 876
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 587, 961
Tru1I TTAA 5 cut(s) 221, 468, 627, 879, 1011
Tru9I TTAA 5 cut(s) 221, 468, 627, 879, 1011
TscAI CASTG 2 cut(s) 276, 846
TseI GCWGC 5 cut(s) 347, 566, 801, 985, 1017
TspDTI ATGAA 3 cut(s) 176, 419, 827
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 2 cut(s) 276, 846
Vha464I CTTAAG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 357
XagI CCTNNNNNAGG 2 cut(s) 366, 907
XapI RAATTY 3 cut(s) 75, 737, 817
XbaI TCTAGA 1 cut(s) 793
XspI CTAG 2 cut(s) 794, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.