Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G44180 AT2G44180 AT3G59990 AT3G59990 AT3G59990 AT3G59990 |
| fragaria_vesca | FvH4_5g31110 FvH4_5g31110 FvH4_5g31120 |
| malus_domestica | MD17G1285400.v1.1 |
| prunus_persica | Prupe.2G330300_v2.0.a1 Prupe.2G330300_v2.0.a1 Prupe.2G330300_v2.0.a1 Prupe.2G330300_v2.0.a1 |
| pyrus_communis | pycom17g28120 |
| rosa_chinensis | RchiOBHm_Chr1g0328111 RchiOBHm_Chr1g0328121 RchiOBHm_Chr1g0328131 |
| rosa_laevigata | RLG00000029939 |
| rosa_multiflora | Rmu_sc0000408.1_g000001 Rmu_sc0002809.1_g000034 Rmu_sc0004646.1_g000013 Rmu_sc0009961.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00060180 Rroxscaffold_4G00321810 |
| rosa_rugosa | Rorug01G0073700 Rorug01G0073800.1 Rorug01G0073900 |
| rosa_samantha | Rh1AG090800 Rh1BG072600 Rh1BG073300 Rh1BG073500 Rh1CG087200 Rh1DG094100 Rh1DG094600 |
| rosa_wichuraiana | Rw1G007030 Rw1G007040 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 260 |
| AciI | CCGC | 1 cut(s) | 115 |
| AcoI | YGGCCR | 2 cut(s) | 19, 245 |
| AcsI | RAATTY | 2 cut(s) | 7, 151 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 121 |
| AflII | CTTAAG | 1 cut(s) | 146 |
| AjnI | CCWGG | 1 cut(s) | 117 |
| AluBI | AGCT | 1 cut(s) | 210 |
| AluI | AGCT | 1 cut(s) | 210 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| AoxI | GGCC | 2 cut(s) | 19, 245 |
| ApeKI | GCWGC | 1 cut(s) | 37 |
| ApoI | RAATTY | 2 cut(s) | 7, 151 |
| AspA2I | CCTAGG | 1 cut(s) | 41 |
| AspLEI | GCGC | 1 cut(s) | 145 |
| AsuNHI | GCTAGC | 1 cut(s) | 58 |
| AvrII | CCTAGG | 1 cut(s) | 41 |
| BbvI | GCAGC | 1 cut(s) | 24 |
| BciT130I | CCWGG | 1 cut(s) | 119 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BfaI | CTAG | 2 cut(s) | 42, 59 |
| BfmI | CTRYAG | 1 cut(s) | 97 |
| BfoI | RGCGCY | 1 cut(s) | 146 |
| BfrI | CTTAAG | 1 cut(s) | 146 |
| BfuAI | ACCTGC | 1 cut(s) | 260 |
| BisI | GCNGC | 1 cut(s) | 38 |
| BlnI | CCTAGG | 1 cut(s) | 41 |
| BlsI | GCNGC | 1 cut(s) | 39 |
| Bme1390I | CCNGG | 1 cut(s) | 119 |
| BmrFI | CCNGG | 1 cut(s) | 119 |
| BmsI | GCATC | 2 cut(s) | 4, 151 |
| BmtI | GCTAGC | 1 cut(s) | 62 |
| BsaI | GGTCTC | 1 cut(s) | 119 |
| BsaJI | CCNNGG | 2 cut(s) | 41, 118 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 116, 146 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 121 |
| Bse118I | RCCGGY | 1 cut(s) | 17 |
| BseBI | CCWGG | 1 cut(s) | 119 |
| BseDI | CCNNGG | 2 cut(s) | 41, 118 |
| BseGI | GGATG | 1 cut(s) | 24 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BseMII | CTCAG | 2 cut(s) | 23, 233 |
| BseRI | GAGGAG | 1 cut(s) | 177 |
| BseXI | GCAGC | 1 cut(s) | 24 |
| BshFI | GGCC | 2 cut(s) | 21, 247 |
| BsiSI | CCGG | 2 cut(s) | 18, 244 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsnI | GGCC | 2 cut(s) | 21, 247 |
| Bso31I | GGTCTC | 1 cut(s) | 119 |
| BspACI | CCGC | 1 cut(s) | 115 |
| BspANI | GGCC | 2 cut(s) | 21, 247 |
| BspCNI | CTCAG | 2 cut(s) | 24, 232 |
| BspMAI | CTGCAG | 1 cut(s) | 101 |
| BspMI | ACCTGC | 1 cut(s) | 260 |
| BspOI | GCTAGC | 1 cut(s) | 62 |
| BspTI | CTTAAG | 1 cut(s) | 146 |
| BspTNI | GGTCTC | 1 cut(s) | 119 |
| BsrFI | RCCGGY | 1 cut(s) | 17 |
| BssAI | RCCGGY | 1 cut(s) | 17 |
| BssECI | CCNNGG | 2 cut(s) | 41, 118 |
| BssT1I | CCWWGG | 1 cut(s) | 41 |
| Bst2UI | CCWGG | 1 cut(s) | 119 |
| BstAFI | CTTAAG | 1 cut(s) | 146 |
| BstC8I | GCNNGC | 4 cut(s) | 19, 60, 101, 166 |
| BstDEI | CTNAG | 2 cut(s) | 32, 219 |
| BstF5I | GGATG | 1 cut(s) | 24 |
| BstH2I | RGCGCY | 1 cut(s) | 146 |
| BstHHI | GCGC | 1 cut(s) | 145 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 165 |
| BstNI | CCWGG | 1 cut(s) | 119 |
| BstSCI | CCNGG | 1 cut(s) | 117 |
| BstSFI | CTRYAG | 1 cut(s) | 97 |
| BstV1I | GCAGC | 1 cut(s) | 24 |
| BsuRI | GGCC | 2 cut(s) | 21, 247 |
| BtgZI | GCGATG | 1 cut(s) | 174 |
| BtsCI | GGATG | 1 cut(s) | 24 |
| BtsIMutI | CAGTG | 1 cut(s) | 29 |
| BveI | ACCTGC | 1 cut(s) | 260 |
| Cac8I | GCNNGC | 4 cut(s) | 19, 60, 101, 166 |
| CfoI | GCGC | 1 cut(s) | 145 |
| Cfr10I | RCCGGY | 1 cut(s) | 17 |
| CviJI | RGCY | 5 cut(s) | 21, 37, 178, 210, 247 |
| CviKI_1 | RGCY | 5 cut(s) | 21, 37, 178, 210, 247 |
| DdeI | CTNAG | 2 cut(s) | 32, 219 |
| EaeI | YGGCCR | 2 cut(s) | 19, 245 |
| Eco130I | CCWWGG | 1 cut(s) | 41 |
| Eco31I | GGTCTC | 1 cut(s) | 119 |
| EcoRII | CCWGG | 1 cut(s) | 117 |
| EcoT14I | CCWWGG | 1 cut(s) | 41 |
| ErhI | CCWWGG | 1 cut(s) | 41 |
| FaiI | YATR | 4 cut(s) | 140, 213, 231, 266 |
| Fnu4HI | GCNGC | 1 cut(s) | 38 |
| FokI | GGATG | 1 cut(s) | 11 |
| Fsp4HI | GCNGC | 1 cut(s) | 38 |
| FspBI | CTAG | 2 cut(s) | 42, 59 |
| GlaI | GCGC | 1 cut(s) | 144 |
| GluI | GCNGC | 1 cut(s) | 38 |
| HaeII | RGCGCY | 1 cut(s) | 146 |
| HaeIII | GGCC | 2 cut(s) | 21, 247 |
| HapII | CCGG | 2 cut(s) | 18, 244 |
| HhaI | GCGC | 1 cut(s) | 145 |
| Hin6I | GCGC | 1 cut(s) | 143 |
| HinP1I | GCGC | 1 cut(s) | 143 |
| HpaII | CCGG | 2 cut(s) | 18, 244 |
| Hpy188III | TCNNGA | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 2 cut(s) | 99, 255 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 165 |
| HpyF3I | CTNAG | 2 cut(s) | 32, 219 |
| HspAI | GCGC | 1 cut(s) | 143 |
| KroI | GCCGGC | 1 cut(s) | 17 |
| KroNI | GCCGGC | 1 cut(s) | 19 |
| LmnI | GCTCC | 1 cut(s) | 207 |
| LpnPI | CCDG | 8 cut(s) | 31, 85, 104, 131, 150, 188, 257, 265 |
| Lsp1109I | GCAGC | 1 cut(s) | 24 |
| LweI | GCATC | 2 cut(s) | 4, 151 |
| MaeI | CTAG | 2 cut(s) | 42, 59 |
| MfeI | CAATTG | 1 cut(s) | 53 |
| MluCI | AATT | 5 cut(s) | 7, 53, 66, 151, 234 |
| MnlI | CCTC | 5 cut(s) | 27, 195, 198, 253, 266 |
| MroNI | GCCGGC | 1 cut(s) | 17 |
| MseI | TTAA | 3 cut(s) | 69, 147, 201 |
| MspCI | CTTAAG | 1 cut(s) | 146 |
| MspI | CCGG | 2 cut(s) | 18, 244 |
| MspR9I | CCNGG | 1 cut(s) | 119 |
| MunI | CAATTG | 1 cut(s) | 53 |
| MvaI | CCWGG | 1 cut(s) | 119 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 165 |
| NaeI | GCCGGC | 1 cut(s) | 19 |
| NgoMIV | GCCGGC | 1 cut(s) | 17 |
| NheI | GCTAGC | 1 cut(s) | 58 |
| PdiI | GCCGGC | 1 cut(s) | 19 |
| PkrI | GCNGC | 1 cut(s) | 39 |
| Psp6I | CCWGG | 1 cut(s) | 117 |
| PspGI | CCWGG | 1 cut(s) | 117 |
| PstI | CTGCAG | 1 cut(s) | 101 |
| SaqAI | TTAA | 3 cut(s) | 69, 147, 201 |
| SatI | GCNGC | 1 cut(s) | 38 |
| ScrFI | CCNGG | 1 cut(s) | 119 |
| SetI | ASST | 3 cut(s) | 212, 254, 264 |
| SfaNI | GCATC | 2 cut(s) | 4, 151 |
| SfcI | CTRYAG | 1 cut(s) | 97 |
| SmlI | CTYRAG | 1 cut(s) | 146 |
| SmoI | CTYRAG | 1 cut(s) | 146 |
| Sse9I | AATT | 5 cut(s) | 7, 53, 66, 151, 234 |
| SsiI | CCGC | 1 cut(s) | 115 |
| SspMI | CTAG | 2 cut(s) | 42, 59 |
| StyD4I | CCNGG | 1 cut(s) | 117 |
| StyI | CCWWGG | 1 cut(s) | 41 |
| TasI | AATT | 5 cut(s) | 7, 53, 66, 151, 234 |
| Tru1I | TTAA | 3 cut(s) | 69, 147, 201 |
| Tru9I | TTAA | 3 cut(s) | 69, 147, 201 |
| TscAI | CASTG | 1 cut(s) | 36 |
| TseI | GCWGC | 1 cut(s) | 37 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspRI | CASTG | 1 cut(s) | 36 |
| Vha464I | CTTAAG | 1 cut(s) | 146 |
| XapI | RAATTY | 2 cut(s) | 7, 151 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 136 |
| XmaJI | CCTAGG | 1 cut(s) | 41 |
| XspI | CTAG | 2 cut(s) | 42, 59 |