Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G44180 AT2G44180 AT3G59990 AT3G59990 AT3G59990 AT3G59990 |
| fragaria_vesca | FvH4_5g31110 FvH4_5g31110 FvH4_5g31120 |
| malus_domestica | MD17G1285400.v1.1 |
| prunus_persica | Prupe.2G330300_v2.0.a1 Prupe.2G330300_v2.0.a1 Prupe.2G330300_v2.0.a1 Prupe.2G330300_v2.0.a1 |
| pyrus_communis | pycom17g28120 |
| rosa_chinensis | RchiOBHm_Chr1g0328111 RchiOBHm_Chr1g0328121 RchiOBHm_Chr1g0328131 |
| rosa_laevigata | RLG00000029939 |
| rosa_multiflora | Rmu_sc0000408.1_g000001 Rmu_sc0002809.1_g000034 Rmu_sc0004646.1_g000013 Rmu_sc0009961.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00060180 Rroxscaffold_4G00321810 |
| rosa_rugosa | Rorug01G0073700 Rorug01G0073800.1 Rorug01G0073900 |
| rosa_samantha | Rh1AG090800 Rh1BG072600 Rh1BG073300 Rh1BG073500 Rh1CG087200 Rh1DG094100 Rh1DG094600 |
| rosa_wichuraiana | Rw1G007030 Rw1G007040 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 360 |
| AcuI | CTGAAG | 1 cut(s) | 255 |
| AcvI | CACGTG | 1 cut(s) | 387 |
| AfaI | GTAC | 2 cut(s) | 82, 415 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 252 |
| AgsI | TTSAA | 4 cut(s) | 30, 221, 353, 378 |
| AjnI | CCWGG | 2 cut(s) | 76, 110 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 32 |
| AluBI | AGCT | 1 cut(s) | 60 |
| AluI | AGCT | 1 cut(s) | 60 |
| Alw26I | GTCTC | 1 cut(s) | 132 |
| AlwI | GGATC | 1 cut(s) | 360 |
| ApeKI | GCWGC | 2 cut(s) | 60, 231 |
| AspLEI | GCGC | 1 cut(s) | 25 |
| AspS9I | GGNCC | 1 cut(s) | 241 |
| AvaII | GGWCC | 1 cut(s) | 241 |
| BbrPI | CACGTG | 1 cut(s) | 387 |
| BbvI | GCAGC | 2 cut(s) | 47, 218 |
| BccI | CCATC | 1 cut(s) | 309 |
| BciT130I | CCWGG | 2 cut(s) | 78, 112 |
| BcoDI | GTCTC | 1 cut(s) | 132 |
| BfmI | CTRYAG | 1 cut(s) | 61 |
| BfoI | RGCGCY | 1 cut(s) | 26 |
| BisI | GCNGC | 2 cut(s) | 61, 232 |
| BlsI | GCNGC | 2 cut(s) | 62, 233 |
| Bme1390I | CCNGG | 2 cut(s) | 78, 112 |
| Bme18I | GGWCC | 1 cut(s) | 241 |
| BmgT120I | GGNCC | 1 cut(s) | 241 |
| BmiI | GGNNCC | 1 cut(s) | 243 |
| BmrFI | CCNGG | 2 cut(s) | 78, 112 |
| BmsI | GCATC | 1 cut(s) | 201 |
| BsaAI | YACGTR | 1 cut(s) | 387 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 252 |
| Bse3DI | GCAATG | 1 cut(s) | 193 |
| BseBI | CCWGG | 2 cut(s) | 78, 112 |
| BseGI | GGATG | 1 cut(s) | 216 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 252 |
| BseMI | GCAATG | 1 cut(s) | 193 |
| BseRI | GAGGAG | 1 cut(s) | 415 |
| BseXI | GCAGC | 2 cut(s) | 47, 218 |
| BslFI | GGGAC | 1 cut(s) | 270 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 252 |
| BsmAI | GTCTC | 1 cut(s) | 132 |
| BsmFI | GGGAC | 1 cut(s) | 270 |
| BsmI | GAATGC | 2 cut(s) | 198, 348 |
| Bsp143I | GATC | 1 cut(s) | 365 |
| BspLI | GGNNCC | 1 cut(s) | 243 |
| BspMAI | CTGCAG | 1 cut(s) | 65 |
| BspPI | GGATC | 1 cut(s) | 360 |
| BsrDI | GCAATG | 1 cut(s) | 193 |
| BssMI | GATC | 1 cut(s) | 365 |
| Bst2UI | CCWGG | 2 cut(s) | 78, 112 |
| Bst4CI | ACNGT | 4 cut(s) | 155, 266, 333, 344 |
| Bst6I | CTCTTC | 1 cut(s) | 3 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 195 |
| BstBAI | YACGTR | 1 cut(s) | 387 |
| BstC8I | GCNNGC | 1 cut(s) | 191 |
| BstDEI | CTNAG | 1 cut(s) | 427 |
| BstF5I | GGATG | 1 cut(s) | 216 |
| BstH2I | RGCGCY | 1 cut(s) | 26 |
| BstHHI | GCGC | 1 cut(s) | 25 |
| BstKTI | GATC | 1 cut(s) | 368 |
| BstMAI | GTCTC | 1 cut(s) | 132 |
| BstMBI | GATC | 1 cut(s) | 365 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 195 |
| BstNI | CCWGG | 2 cut(s) | 78, 112 |
| BstSCI | CCNGG | 2 cut(s) | 76, 110 |
| BstSFI | CTRYAG | 1 cut(s) | 61 |
| BstV1I | GCAGC | 2 cut(s) | 47, 218 |
| BtgZI | GCGATG | 1 cut(s) | 57 |
| BtsCI | GGATG | 1 cut(s) | 216 |
| Cac8I | GCNNGC | 1 cut(s) | 191 |
| CfoI | GCGC | 1 cut(s) | 25 |
| Cfr13I | GGNCC | 1 cut(s) | 241 |
| Csp6I | GTAC | 2 cut(s) | 81, 414 |
| CviAII | CATG | 1 cut(s) | 309 |
| CviJI | RGCY | 3 cut(s) | 60, 110, 381 |
| CviKI_1 | RGCY | 3 cut(s) | 60, 110, 381 |
| CviQI | GTAC | 2 cut(s) | 81, 414 |
| DdeI | CTNAG | 1 cut(s) | 427 |
| DpnI | GATC | 1 cut(s) | 367 |
| DpnII | GATC | 1 cut(s) | 365 |
| DraI | TTTAAA | 1 cut(s) | 106 |
| Eam1104I | CTCTTC | 1 cut(s) | 3 |
| EarI | CTCTTC | 1 cut(s) | 3 |
| Eco47I | GGWCC | 1 cut(s) | 241 |
| Eco57I | CTGAAG | 1 cut(s) | 255 |
| Eco72I | CACGTG | 1 cut(s) | 387 |
| EcoRII | CCWGG | 2 cut(s) | 76, 110 |
| FaeI | CATG | 1 cut(s) | 312 |
| FaiI | YATR | 7 cut(s) | 41, 43, 277, 310, 322, 324, 360 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 402, 434 |
| FaqI | GGGAC | 1 cut(s) | 270 |
| FatI | CATG | 1 cut(s) | 308 |
| Fnu4HI | GCNGC | 2 cut(s) | 61, 232 |
| FokI | GGATG | 1 cut(s) | 223 |
| Fsp4HI | GCNGC | 2 cut(s) | 61, 232 |
| GlaI | GCGC | 1 cut(s) | 24 |
| GluI | GCNGC | 2 cut(s) | 61, 232 |
| HaeII | RGCGCY | 1 cut(s) | 26 |
| HhaI | GCGC | 1 cut(s) | 25 |
| Hin1II | CATG | 1 cut(s) | 312 |
| Hin6I | GCGC | 1 cut(s) | 23 |
| HinP1I | GCGC | 1 cut(s) | 23 |
| HincII | GTYRAC | 1 cut(s) | 329 |
| HindII | GTYRAC | 1 cut(s) | 329 |
| Hpy166II | GTNNAC | 1 cut(s) | 329 |
| Hpy188I | TCNGA | 2 cut(s) | 96, 173 |
| Hpy188III | TCNNGA | 1 cut(s) | 183 |
| Hpy8I | GTNNAC | 1 cut(s) | 329 |
| Hpy99I | CGWCG | 1 cut(s) | 58 |
| HpyCH4III | ACNGT | 4 cut(s) | 155, 266, 333, 344 |
| HpyCH4IV | ACGT | 1 cut(s) | 386 |
| HpyCH4V | TGCA | 3 cut(s) | 63, 198, 336 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 195 |
| HpyF3I | CTNAG | 1 cut(s) | 427 |
| HpySE526I | ACGT | 1 cut(s) | 386 |
| Hsp92II | CATG | 1 cut(s) | 312 |
| HspAI | GCGC | 1 cut(s) | 23 |
| Kzo9I | GATC | 1 cut(s) | 365 |
| LmnI | GCTCC | 1 cut(s) | 20 |
| LpnPI | CCDG | 8 cut(s) | 63, 90, 97, 124, 168, 175, 193, 238 |
| Lsp1109I | GCAGC | 2 cut(s) | 47, 218 |
| LweI | GCATC | 1 cut(s) | 201 |
| MaeII | ACGT | 1 cut(s) | 386 |
| MalI | GATC | 1 cut(s) | 367 |
| MboI | GATC | 1 cut(s) | 365 |
| MboII | GAAGA | 2 cut(s) | 15, 20 |
| MluCI | AATT | 3 cut(s) | 44, 221, 291 |
| MnlI | CCTC | 5 cut(s) | 4, 90, 392, 433, 436 |
| MseI | TTAA | 3 cut(s) | 105, 165, 434 |
| MslI | CAYNNNNRTG | 1 cut(s) | 313 |
| MspR9I | CCNGG | 2 cut(s) | 78, 112 |
| Mva1269I | GAATGC | 2 cut(s) | 198, 348 |
| MvaI | CCWGG | 2 cut(s) | 78, 112 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 195 |
| NdeII | GATC | 1 cut(s) | 365 |
| NlaIII | CATG | 1 cut(s) | 312 |
| NlaIV | GGNNCC | 1 cut(s) | 243 |
| PctI | GAATGC | 2 cut(s) | 198, 348 |
| PkrI | GCNGC | 2 cut(s) | 62, 233 |
| PmaCI | CACGTG | 1 cut(s) | 387 |
| PmlI | CACGTG | 1 cut(s) | 387 |
| Ppu21I | YACGTR | 1 cut(s) | 387 |
| Psp6I | CCWGG | 2 cut(s) | 76, 110 |
| PspCI | CACGTG | 1 cut(s) | 387 |
| PspGI | CCWGG | 2 cut(s) | 76, 110 |
| PspN4I | GGNNCC | 1 cut(s) | 243 |
| PspPI | GGNCC | 1 cut(s) | 241 |
| PstI | CTGCAG | 1 cut(s) | 65 |
| RsaI | GTAC | 2 cut(s) | 82, 415 |
| RsaNI | GTAC | 2 cut(s) | 81, 414 |
| RseI | CAYNNNNRTG | 1 cut(s) | 313 |
| SaqAI | TTAA | 3 cut(s) | 105, 165, 434 |
| SatI | GCNGC | 2 cut(s) | 61, 232 |
| Sau3AI | GATC | 1 cut(s) | 365 |
| Sau96I | GGNCC | 1 cut(s) | 241 |
| ScrFI | CCNGG | 2 cut(s) | 78, 112 |
| SetI | ASST | 5 cut(s) | 62, 82, 389, 415, 425 |
| SfaNI | GCATC | 1 cut(s) | 201 |
| SfcI | CTRYAG | 1 cut(s) | 61 |
| SinI | GGWCC | 1 cut(s) | 241 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 313 |
| Sse9I | AATT | 3 cut(s) | 44, 221, 291 |
| StyD4I | CCNGG | 2 cut(s) | 76, 110 |
| TaaI | ACNGT | 4 cut(s) | 155, 266, 333, 344 |
| TaiI | ACGT | 1 cut(s) | 389 |
| TaqI | TCGA | 1 cut(s) | 56 |
| TasI | AATT | 3 cut(s) | 44, 221, 291 |
| Tru1I | TTAA | 3 cut(s) | 105, 165, 434 |
| Tru9I | TTAA | 3 cut(s) | 105, 165, 434 |
| TseI | GCWGC | 2 cut(s) | 60, 231 |
| TspDTI | ATGAA | 2 cut(s) | 60, 303 |
| TspGWI | ACGGA | 2 cut(s) | 98, 147 |
| VpaK11BI | GGWCC | 1 cut(s) | 241 |