RchiOBHm_Chr1g0328121

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003170
Physical Location & Seq
Forward (+)
17409516 .. 17415611
6096 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55757

Sequence Viewer

Length: 1059 bp
ATGGCAGGGGAAAAATTGGATGAAAATATTCCTTGTGAGGAAAATGGGACTGTAGAATCTTCGAATGGCAATGGTAATGGAGAAGCCGCTCAACAATCTCCAACTGTTGAGAAAGATGAGGATGACGACAAAGATGTTTCAAAAAAGAAGAAGAAGAAAAATAAGAGCAAGAAAAAGAAAGCACAGGCAGAGCATACTGATCCACAATCTTTAAGTGGGCAAACTGATCCACAATCTACAAGTAGGCAAACTGCTCCACAATCTACAAGAAGGCAAACTGATCCACCTTCCATTCCTCTTATTGATCTGTTCCCACATGGGGAGTTTCCTGAGGGCGAAATTCAACAATACAGAGATGACAACTTGTGGAGGACTACTTCTGAAGAGAAGAGGGAGTTGGAGCGCCTTGAAAAACCATTATATAATTCAGTTCGTCGAGCTGCAGAAGTTCATCGCCAGGTACGGAAATACATCAAAGGGATTTTAAAGCCTGGAATGTTGATGACTGATTTGTGTGAGACTTTGGAGAACACAGTCCGTAAGTTAATATCCGAGAATGGTCTGGAAGCAGGCATTGCATTCCCCACAGGATGCTCTTTGAATTGGGTTGCTGCTCATTGGACCCCAAATACAGGGGACAAGACGGTGCTTCAGTATGATGATGTGATGAAATTAGACTTTGGAACTCATGTTGATGGTCATATAGTTGACTGTGCATTTACAGTAGCATTCAATCCTATGTTTGATCCACTACTTGAAGCCTCACGTGAAGCAACAAATACGGGTATCAAGGAGTCTGGAATTGATGTGCGTCTTTGTGATGTTGGTGCTGCAATCCAAGAAGTCATGGAATCATATGAGGTTGAAATAAATGGGAAGGTGTATCAAGTTAAAAGTATCCGGAACTTGAATGGACATAGCATTGGACCTTACCAAATCCATGCTGGAAAATCAGTTCCCATTGTGAAAGGAGGGGAGCAGACAAAATGGAAGAGGGTGAATTTTTTGCGATTGAAACTTTCGCATCAACTGGGAAAGGATATGTCAGAGAAGATCTAG

Protein Analysis

352

Amino Acids

39.37

Weight (kDa)

6.05

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 144 - 323 8.3e-23 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 89
AccIII TCCGGA 1 cut(s) 900
AciI CCGC 1 cut(s) 87
AclWI GGATC 4 cut(s) 194, 221, 275, 740
AcsI RAATTY 2 cut(s) 339, 1000
AcuI CTGAAG 2 cut(s) 402, 635
AcvI CACGTG 1 cut(s) 767
AfaI GTAC 1 cut(s) 462
AfiI CCNNNNNNNGG 2 cut(s) 319, 632
AgsI TTSAA 9 cut(s) 141, 344, 410, 601, 733, 758, 866, 910, 1015
AjnI CCWGG 2 cut(s) 456, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AloI GAACNNNNNNTCC 2 cut(s) 939, 971
AluBI AGCT 1 cut(s) 440
AluI AGCT 1 cut(s) 440
Alw26I GTCTC 1 cut(s) 512
AlwI GGATC 4 cut(s) 194, 221, 275, 740
Aor13HI TCCGGA 1 cut(s) 900
ApeKI GCWGC 3 cut(s) 440, 611, 830
ApoI RAATTY 2 cut(s) 339, 1000
Asp700I GAANNNNTTC 1 cut(s) 27
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 2 cut(s) 621, 926
AsuHPI GGTGA 1 cut(s) 1009
AsuII TTCGAA 1 cut(s) 62
AvaII GGWCC 2 cut(s) 621, 926
AxyI CCTNAGG 1 cut(s) 330
BbrPI CACGTG 1 cut(s) 767
BbvI GCAGC 3 cut(s) 427, 598, 817
BccI CCATC 1 cut(s) 689
BciT130I CCWGG 2 cut(s) 458, 492
BciVI GTATCC 1 cut(s) 908
BcoDI GTCTC 1 cut(s) 512
BfaI CTAG 1 cut(s) 1057
BfmI CTRYAG 2 cut(s) 51, 441
BfoI RGCGCY 1 cut(s) 406
BfuI GTATCC 1 cut(s) 908
BglII AGATCT 1 cut(s) 1053
BisI GCNGC 4 cut(s) 87, 441, 612, 831
BlsI GCNGC 4 cut(s) 88, 442, 613, 832
Bme1390I CCNGG 2 cut(s) 458, 492
Bme18I GGWCC 2 cut(s) 621, 926
BmgT120I GGNCC 2 cut(s) 621, 926
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 2 cut(s) 458, 492
BmrI ACTGGG 1 cut(s) 1040
BmsI GCATC 2 cut(s) 581, 1033
BmuI ACTGGG 1 cut(s) 1040
Bpu14I TTCGAA 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 767
BsaWI WCCGGW 1 cut(s) 900
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 632
Bse1I ACTGG 1 cut(s) 1035
Bse21I CCTNAGG 1 cut(s) 330
Bse3DI GCAATG 2 cut(s) 76, 573
BseAI TCCGGA 1 cut(s) 900
BseBI CCWGG 2 cut(s) 458, 492
BseGI GGATG 3 cut(s) 25, 127, 596
BseLI CCNNNNNNNGG 2 cut(s) 319, 632
BseMI GCAATG 2 cut(s) 76, 573
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 1 cut(s) 1035
BseXI GCAGC 3 cut(s) 427, 598, 817
BsiSI CCGG 1 cut(s) 901
BslFI GGGAC 2 cut(s) 61, 650
BslI CCNNNNNNNGG 2 cut(s) 319, 632
BsmAI GTCTC 1 cut(s) 512
BsmFI GGGAC 2 cut(s) 61, 650
BsmI GAATGC 2 cut(s) 578, 728
Bsp119I TTCGAA 1 cut(s) 62
Bsp13I TCCGGA 1 cut(s) 900
Bsp143I GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
BspACI CCGC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 322
BspEI TCCGGA 1 cut(s) 900
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 4 cut(s) 194, 221, 275, 740
BspT104I TTCGAA 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 76, 573
BsrI ACTGG 1 cut(s) 1035
BssMI GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
Bst2UI CCWGG 2 cut(s) 458, 492
Bst4CI ACNGT 6 cut(s) 52, 106, 535, 646, 713, 724
Bst6I CTCTTC 3 cut(s) 378, 383, 986
BstAPI GCANNNNNTGC 1 cut(s) 575
BstBAI YACGTR 1 cut(s) 767
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 571
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 3 cut(s) 25, 127, 596
BstH2I RGCGCY 1 cut(s) 406
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 6 cut(s) 202, 229, 283, 307, 748, 1056
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
BstMWI GCNNNNNNNGC 1 cut(s) 575
BstNI CCWGG 2 cut(s) 458, 492
BstSCI CCNGG 2 cut(s) 456, 490
BstSFI CTRYAG 2 cut(s) 51, 441
BstV1I GCAGC 3 cut(s) 427, 598, 817
BstX2I RGATCY 1 cut(s) 1053
BstYI RGATCY 1 cut(s) 1053
Bsu36I CCTNAGG 1 cut(s) 330
BsuI GTATCC 1 cut(s) 908
BtgZI GCGATG 1 cut(s) 437
BtsCI GGATG 3 cut(s) 25, 127, 596
Cac8I GCNNGC 1 cut(s) 571
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 2 cut(s) 621, 926
CseI GACGC 1 cut(s) 800
Csp6I GTAC 1 cut(s) 461
CviAII CATG 4 cut(s) 317, 689, 847, 941
CviJI RGCY 4 cut(s) 86, 440, 490, 761
CviKI_1 RGCY 4 cut(s) 86, 440, 490, 761
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 1 cut(s) 330
DpnI GATC 6 cut(s) 201, 228, 282, 306, 747, 1055
DpnII GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
DraI TTTAAA 1 cut(s) 486
Eam1104I CTCTTC 3 cut(s) 378, 383, 986
EarI CTCTTC 3 cut(s) 378, 383, 986
Eco47I GGWCC 2 cut(s) 621, 926
Eco57I CTGAAG 2 cut(s) 402, 635
Eco72I CACGTG 1 cut(s) 767
Eco81I CCTNAGG 1 cut(s) 330
EcoRII CCWGG 2 cut(s) 456, 490
FaeI CATG 4 cut(s) 320, 692, 850, 944
FaqI GGGAC 2 cut(s) 61, 650
FatI CATG 4 cut(s) 316, 688, 846, 940
FauNDI CATATG 1 cut(s) 856
Fnu4HI GCNGC 4 cut(s) 87, 441, 612, 831
FokI GGATG 3 cut(s) 32, 134, 603
Fsp4HI GCNGC 4 cut(s) 87, 441, 612, 831
FspBI CTAG 1 cut(s) 1057
GlaI GCGC 1 cut(s) 404
GluI GCNGC 4 cut(s) 87, 441, 612, 831
HaeII RGCGCY 1 cut(s) 406
HapII CCGG 1 cut(s) 901
HgaI GACGC 1 cut(s) 800
HhaI GCGC 1 cut(s) 405
Hin1II CATG 4 cut(s) 320, 692, 850, 944
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 3 cut(s) 56, 794, 851
HpaII CCGG 1 cut(s) 901
HphI GGTGA 1 cut(s) 1009
Hpy166II GTNNAC 1 cut(s) 709
Hpy188I TCNGA 3 cut(s) 382, 553, 1048
Hpy188III TCNNGA 4 cut(s) 329, 563, 798, 901
Hpy8I GTNNAC 1 cut(s) 709
Hpy99I CGWCG 1 cut(s) 438
HpyAV CCTTC 3 cut(s) 264, 297, 871
HpyCH4III ACNGT 6 cut(s) 52, 106, 535, 646, 713, 724
HpyCH4IV ACGT 1 cut(s) 766
HpyCH4V TGCA 4 cut(s) 443, 578, 716, 833
HpyF10VI GCNNNNNNNGC 1 cut(s) 575
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 1 cut(s) 766
Hsp92II CATG 4 cut(s) 320, 692, 850, 944
HspAI GCGC 1 cut(s) 403
Kpn2I TCCGGA 1 cut(s) 900
Kzo9I GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
LmnI GCTCC 3 cut(s) 259, 400, 976
Lsp1109I GCAGC 3 cut(s) 427, 598, 817
LweI GCATC 2 cut(s) 581, 1033
MaeI CTAG 1 cut(s) 1057
MaeII ACGT 1 cut(s) 766
MalI GATC 6 cut(s) 201, 228, 282, 306, 747, 1055
MbiI CCGCTC 1 cut(s) 89
MboI GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
MboII GAAGA 7 cut(s) 51, 160, 163, 166, 395, 400, 1003
MflI RGATCY 1 cut(s) 1053
MluCI AATT 7 cut(s) 14, 339, 424, 601, 671, 801, 1000
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 2 cut(s) 125, 378
MroI TCCGGA 1 cut(s) 900
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 4 cut(s) 212, 485, 545, 891
MslI CAYNNNNRTG 1 cut(s) 693
MspI CCGG 1 cut(s) 901
MspR9I CCNGG 2 cut(s) 458, 492
Mva1269I GAATGC 2 cut(s) 578, 728
MvaI CCWGG 2 cut(s) 458, 492
MwoI GCNNNNNNNGC 1 cut(s) 575
NdeI CATATG 1 cut(s) 856
NdeII GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
NlaIII CATG 4 cut(s) 320, 692, 850, 944
NlaIV GGNNCC 1 cut(s) 623
NspV TTCGAA 1 cut(s) 62
PctI GAATGC 2 cut(s) 578, 728
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 56, 851
PkrI GCNGC 4 cut(s) 88, 442, 613, 832
PleI GAGTC 1 cut(s) 802
PmaCI CACGTG 1 cut(s) 767
PmlI CACGTG 1 cut(s) 767
PpsI GAGTC 1 cut(s) 802
Ppu21I YACGTR 1 cut(s) 767
Psp6I CCWGG 2 cut(s) 456, 490
PspCI CACGTG 1 cut(s) 767
PspGI CCWGG 2 cut(s) 456, 490
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 2 cut(s) 621, 926
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 1053
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
RseI CAYNNNNRTG 1 cut(s) 693
SaqAI TTAA 4 cut(s) 212, 485, 545, 891
SatI GCNGC 4 cut(s) 87, 441, 612, 831
Sau3AI GATC 6 cut(s) 199, 226, 280, 304, 745, 1053
Sau96I GGNCC 2 cut(s) 621, 926
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 2 cut(s) 458, 492
SetI ASST 7 cut(s) 289, 442, 462, 769, 864, 882, 931
SfaNI GCATC 2 cut(s) 581, 1033
SfcI CTRYAG 2 cut(s) 51, 441
SfuI TTCGAA 1 cut(s) 62
SinI GGWCC 2 cut(s) 621, 926
SmiMI CAYNNNNRTG 1 cut(s) 693
Sse9I AATT 7 cut(s) 14, 339, 424, 601, 671, 801, 1000
SsiI CCGC 1 cut(s) 87
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 1057
StyD4I CCNGG 2 cut(s) 456, 490
TaaI ACNGT 6 cut(s) 52, 106, 535, 646, 713, 724
TaiI ACGT 1 cut(s) 769
TaqI TCGA 2 cut(s) 62, 436
TasI AATT 7 cut(s) 14, 339, 424, 601, 671, 801, 1000
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 2 cut(s) 56, 851
Tru1I TTAA 4 cut(s) 212, 485, 545, 891
Tru9I TTAA 4 cut(s) 212, 485, 545, 891
TseI GCWGC 3 cut(s) 440, 611, 830
TspDTI ATGAA 3 cut(s) 36, 440, 683
TspGWI ACGGA 2 cut(s) 478, 527
VpaK11BI GGWCC 2 cut(s) 621, 926
XapI RAATTY 2 cut(s) 339, 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.