AT4G20440

Small nuclear

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001710
Physical Location & Seq
Reverse (-)
11022036 .. 11023851
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20440.4

Sequence Viewer

Length: 774 bp
ATGTCGATGTCGAAGAGTTCAAAGATGCTTCAGTTCATCAACTACAGGATGCGAGTGACGATCCAAGACGGAAGACAGCTCGTTGGGAAGTTCATGGCGTTTGACCGTCACATGAACCTCGTTCTCGGCGATTGCGAGGAGTTTCGTAAGCTTCCACCAGCTAAAGGGAAGAAGATCAACGAAGAGCGTGAAGATCGCCGTACGCTAGGTTTAGTGTTGCTTAGAGGTGAAGAAGTTATCTCAATGACTGTCGAAGGACCACCTCCTCCTGAAGAATCTCGTGCTAAAGCTGGCTCTGCAGCTGCTGTTGCTGGTCCAGGAATTGGCCGTGCCGCTGGGCGTGGAGTTCCTACTGGTCCGTTAGTTCAAGCTCAGCCTGGTTTATCTGGTCCTGTTCGTGGTGTTGGTGGACCTGCTCCGGGAATGATGCAGCCTCAGATCTCTCGTCCACCACAGCTTTCAGCTCCTCCGATTATTCGACCTCCGGGACAGATGTTGCCACCGCCTCCTTTTGGTGGTCAAGGTCCTCCTATGGGAAGAGGTCCTCCACCGCCTTATGGTATGAGGCCACCGCCACAGCAGTTTTCTGGACCACCACCGCCTCAGTATGGGCAAAGGCCAATGATTCCTCCTCCTGGTGGTATGATGAGAGGACCTCCTCCTCCACCTCATGGAATGCAAGGACCTCCTCCGCCTCGCCCTGGAATGCCTCCTGCTCCTGGTGGTTTTGCTCCACCGCGTCCTGGCATGCCACCACATAATCAGCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05322 map03040 map05322
Pfam Domains
Protein Families
LSM

Protein Analysis

257

Amino Acids

27.14

Weight (kDa)

11.17

Isoelectric Point (pI)

69.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 9 - 83 9.2e-17 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 421
AccB7I CCANNNNNTGG 2 cut(s) 323, 671
AccII CGCG 1 cut(s) 739
AciI CCGC 7 cut(s) 333, 503, 551, 572, 599, 692, 737
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 325
AcuI CTGAAG 2 cut(s) 14, 291
AfaI GTAC 1 cut(s) 202
AgsI TTSAA 2 cut(s) 21, 368
AjnI CCWGG 6 cut(s) 316, 376, 634, 700, 718, 742
AluBI AGCT 8 cut(s) 79, 151, 161, 290, 302, 371, 457, 464
AluI AGCT 8 cut(s) 79, 151, 161, 290, 302, 371, 457, 464
AlwI GGATC 1 cut(s) 55
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 3 cut(s) 325, 566, 617
ApeKI GCWGC 4 cut(s) 299, 302, 430, 766
AsuC2I CCSGG 2 cut(s) 420, 486
AsuHPI GGTGA 1 cut(s) 239
BauI CACGAG 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 79
BbvI GCAGC 3 cut(s) 289, 311, 442
BceAI ACGGC 2 cut(s) 183, 312
BciT130I CCWGG 6 cut(s) 318, 378, 636, 702, 720, 744
BcnI CCSGG 2 cut(s) 420, 486
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 2 cut(s) 43, 297
BfuAI ACCTGC 1 cut(s) 421
BglII AGATCT 1 cut(s) 438
BisI GCNGC 5 cut(s) 300, 303, 333, 431, 767
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 5 cut(s) 301, 304, 334, 432, 768
Bme1390I CCNGG 8 cut(s) 318, 378, 420, 486, 636, 702, 720, 744
BmrFI CCNGG 8 cut(s) 318, 378, 420, 486, 636, 702, 720, 744
BmsI GCATC 3 cut(s) 15, 39, 417
BpiI GAAGAC 1 cut(s) 79
BplI GAGNNNNNCTC 2 cut(s) 640, 672
Bpu1102I GCTNAGC 1 cut(s) 372
BpuMI CCSGG 2 cut(s) 420, 486
BsaJI CCNNGG 1 cut(s) 700
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 6 cut(s) 318, 378, 636, 702, 720, 744
BseDI CCNNGG 1 cut(s) 700
BseGI GGATG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 386, 449, 617
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 7 cut(s) 152, 255, 456, 621, 648, 651, 678
BseXI GCAGC 3 cut(s) 289, 311, 442
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 739
BshFI GGCC 3 cut(s) 327, 568, 619
BsiSI CCGG 2 cut(s) 419, 485
BsiWI CGTACG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 501
BsmI GAATGC 2 cut(s) 681, 711
BsnI GGCC 3 cut(s) 327, 568, 619
Bsp143I GATC 4 cut(s) 60, 174, 193, 438
Bsp1720I GCTNAGC 1 cut(s) 372
BspACI CCGC 7 cut(s) 333, 503, 551, 572, 599, 692, 737
BspANI GGCC 3 cut(s) 327, 568, 619
BspCNI CTCAG 3 cut(s) 385, 448, 616
BspFNI CGCG 1 cut(s) 739
BspMAI CTGCAG 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 1 cut(s) 55
BspQI GCTCTTC 1 cut(s) 177
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 700
BssMI GATC 4 cut(s) 60, 174, 193, 438
BssSI CACGAG 1 cut(s) 279
Bst2BI CACGAG 1 cut(s) 279
Bst2UI CCWGG 6 cut(s) 318, 378, 636, 702, 720, 744
Bst4CI ACNGT 2 cut(s) 107, 250
Bst6I CTCTTC 3 cut(s) 8, 177, 532
BstC8I GCNNGC 2 cut(s) 292, 749
BstDEI CTNAG 4 cut(s) 221, 372, 435, 603
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 1 cut(s) 739
BstKTI GATC 4 cut(s) 63, 177, 196, 441
BstMBI GATC 4 cut(s) 60, 174, 193, 438
BstMWI GCNNNNNNNGC 2 cut(s) 296, 308
BstNI CCWGG 6 cut(s) 318, 378, 636, 702, 720, 744
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 8 cut(s) 316, 376, 418, 484, 634, 700, 718, 742
BstSFI CTRYAG 2 cut(s) 43, 297
BstUI CGCG 1 cut(s) 739
BstV1I GCAGC 3 cut(s) 289, 311, 442
BstV2I GAAGAC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 3 cut(s) 327, 568, 619
BtsCI GGATG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 2 cut(s) 292, 749
CaiI CAGNNNCTG 1 cut(s) 305
CseI GACGC 1 cut(s) 728
Csp6I GTAC 1 cut(s) 201
CviAII CATG 4 cut(s) 94, 112, 671, 748
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 4 cut(s) 221, 372, 435, 603
DpnI GATC 4 cut(s) 62, 176, 195, 440
DpnII GATC 4 cut(s) 60, 174, 193, 438
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 3 cut(s) 8, 177, 532
EarI CTCTTC 3 cut(s) 8, 177, 532
EciI GGCGGA 1 cut(s) 681
Eco57I CTGAAG 2 cut(s) 14, 291
EcoO109I RGGNCCY 4 cut(s) 524, 542, 653, 683
EcoRII CCWGG 6 cut(s) 316, 376, 634, 700, 718, 742
FaeI CATG 4 cut(s) 97, 115, 674, 751
FaqI GGGAC 1 cut(s) 501
FatI CATG 4 cut(s) 93, 111, 670, 747
Fnu4HI GCNGC 5 cut(s) 300, 303, 333, 431, 767
FokI GGATG 1 cut(s) 61
Fsp4HI GCNGC 5 cut(s) 300, 303, 333, 431, 767
FspBI CTAG 1 cut(s) 206
GluI GCNGC 5 cut(s) 300, 303, 333, 431, 767
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 3 cut(s) 327, 568, 619
HapII CCGG 2 cut(s) 419, 485
HgaI GACGC 1 cut(s) 728
Hin1II CATG 4 cut(s) 97, 115, 674, 751
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 2 cut(s) 275, 625
HpaII CCGG 2 cut(s) 419, 485
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 410, 449
Hpy188I TCNGA 2 cut(s) 438, 471
Hpy188III TCNNGA 2 cut(s) 269, 588
Hpy8I GTNNAC 2 cut(s) 410, 449
HpyAV CCTTC 1 cut(s) 248
HpyCH4III ACNGT 2 cut(s) 107, 250
HpyCH4V TGCA 3 cut(s) 299, 430, 679
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 308
HpyF3I CTNAG 4 cut(s) 221, 372, 435, 603
Hsp92II CATG 4 cut(s) 97, 115, 674, 751
Kzo9I GATC 4 cut(s) 60, 174, 193, 438
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 4 cut(s) 421, 469, 721, 736
Lsp1109I GCAGC 3 cut(s) 289, 311, 442
LweI GCATC 3 cut(s) 15, 39, 417
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 55, 107
MalI GATC 4 cut(s) 62, 176, 195, 440
MboI GATC 4 cut(s) 60, 174, 193, 438
MboII GAAGA 9 cut(s) 25, 84, 181, 184, 194, 203, 242, 284, 549
MflI RGATCY 1 cut(s) 438
MluCI AATT 1 cut(s) 321
MspA1I CMGCKG 2 cut(s) 302, 335
MspI CCGG 2 cut(s) 419, 485
MspR9I CCNGG 8 cut(s) 318, 378, 420, 486, 636, 702, 720, 744
Mva1269I GAATGC 2 cut(s) 681, 711
MvaI CCWGG 6 cut(s) 318, 378, 636, 702, 720, 744
MvnI CGCG 1 cut(s) 739
MwoI GCNNNNNNNGC 2 cut(s) 296, 308
NciI CCSGG 2 cut(s) 420, 486
NdeII GATC 4 cut(s) 60, 174, 193, 438
NlaIII CATG 4 cut(s) 97, 115, 674, 751
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 2 cut(s) 55, 107
NspI RCATGY 1 cut(s) 751
PaeI GCATGC 1 cut(s) 751
PciSI GCTCTTC 1 cut(s) 177
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 132
PctI GAATGC 2 cut(s) 681, 711
PfeI GAWTC 2 cut(s) 275, 625
Pfl23II CGTACG 1 cut(s) 200
PflMI CCANNNNNTGG 2 cut(s) 323, 671
PfoI TCCNGGA 3 cut(s) 316, 418, 484
PkrI GCNGC 5 cut(s) 301, 304, 334, 432, 768
PpuMI RGGWCCY 4 cut(s) 524, 542, 653, 683
Psp5II RGGWCCY 4 cut(s) 524, 542, 653, 683
Psp6I CCWGG 6 cut(s) 316, 376, 634, 700, 718, 742
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 6 cut(s) 316, 376, 634, 700, 718, 742
PspLI CGTACG 1 cut(s) 200
PspPPI RGGWCCY 4 cut(s) 524, 542, 653, 683
PstI CTGCAG 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 438
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 177
SatI GCNGC 5 cut(s) 300, 303, 333, 431, 767
Sau3AI GATC 4 cut(s) 60, 174, 193, 438
ScrFI CCNGG 8 cut(s) 318, 378, 420, 486, 636, 702, 720, 744
SfaNI GCATC 3 cut(s) 15, 39, 417
SfcI CTRYAG 2 cut(s) 43, 297
SphI GCATGC 1 cut(s) 751
Sse9I AATT 1 cut(s) 321
SsiI CCGC 7 cut(s) 333, 503, 551, 572, 599, 692, 737
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 8 cut(s) 316, 376, 418, 484, 634, 700, 718, 742
TaaI ACNGT 2 cut(s) 107, 250
TaqI TCGA 4 cut(s) 5, 11, 252, 478
TasI AATT 1 cut(s) 321
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 2 cut(s) 275, 625
TseFI GTSAC 2 cut(s) 55, 107
TseI GCWGC 4 cut(s) 299, 302, 430, 766
Tsp45I GTSAC 2 cut(s) 55, 107
TspDTI ATGAA 3 cut(s) 25, 82, 128
TspGWI ACGGA 2 cut(s) 84, 348
Van91I CCANNNNNTGG 2 cut(s) 323, 671
XceI RCATGY 1 cut(s) 751
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.