MD14G1174700.v1.1

Small nuclear ribonucleoprotein-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001710
Physical Location & Seq
Reverse (-)
26837886 .. 26840602
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1174700.v1.1.491

Sequence Viewer

Length: 954 bp
ATGTCGATGTCAAAGAGCTCCAAGATGCTGCAATTCATAAACTATCGGATGCGAGTGACGATCCAAGACGGGAGGCAGCTCGTCGGCAAGTTCATGGCCTTCGATCGCCACATGAACCTCGTCCTCGGCGACTGCGAGGAGTTCCGGAAGTTGCCGCCACTGAAAGGCAAGAAGACCAACGAGGAGCGCGACGACCGCCGGACACTAGGCCTCGTACTCCTCCGCGGCGAAGAGGTCGTGTCAATGACCGTCGAGGGCCCGCCTCCTCAGGAAGAGTCTCGAGCTAAGGCAGCCAGCGCCGCCGCTTTGGCTGGTCCAGGATTGGGCCGCGCCGCCGGACGAGGTATCCCCACTGCTCCTCTCGCCCAGGCCCAGCCCGGTCTCTCTGGCCCGGTTCGCGGCGTCGGTGGACCAGCCCCAGGTATGATGCAGCCCCAGATCTCGCGCCCGCCAGTTCCGAACCTCTCCGCGCCGCCTATGTCGTACCCGGCCGCTCCAGTGATTCGTCCACCCGGGCAGATGCCTGGATATCCCGGGCAACCTCCGATGGGTCGTGGTCCACCTCCTGGTGTTCCGCCCCCTCAGTTTGCTAGGCCTCCTGGTGGGGGTCCACCTCAGCAATTCCCTGGCCCGCCACAGATGCAGTTTGGGCAGAGGCCGATGGGACCTCCGCCCCCTGGGCAGATGATGAGGGGTCCACCGCCTCCACCTCGCCCCGGAATGCAAGCTCCCCCACCCCGTCCCGGAATGCCGCCTCCACCTGGCGGTGGAATGCATATGTTTGGACCGCCTCGTCCTGGAATGCCACCTCCACCCAACCCTCAGAACCAGCAGCAAAATCAGCAGCAACAGTTTATTAGATGTGATTTTGAGGTTTTGGAATGCTGCTGCCTTGCATATTGTTTCAGTTTTTGTAATGGGTTTGAGCCTGTGCAGACCTTCCTCATCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05322 map03040 map05322
Pfam Domains
Protein Families
LSM

Protein Analysis

318

Amino Acids

33.9

Weight (kDa)

9.93

Isoelectric Point (pI)

70.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 9 - 83 8.5e-17 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 792
AccB7I CCANNNNNTGG 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 494
AccII CGCG 6 cut(s) 189, 225, 330, 399, 445, 470
AccIII TCCGGA 1 cut(s) 144
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 2 cut(s) 216, 485
AluBI AGCT 4 cut(s) 18, 79, 284, 728
AluI AGCT 4 cut(s) 18, 79, 284, 728
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 2 cut(s) 282, 386
AlwI GGATC 1 cut(s) 55
Ama87I CYCGRG 3 cut(s) 279, 512, 533
Aor13HI TCCGGA 1 cut(s) 144
ApaI GGGCCC 1 cut(s) 260
ApeKI GCWGC 8 cut(s) 28, 76, 290, 430, 832, 844, 885, 888
AspLEI GCGC 5 cut(s) 189, 299, 332, 447, 472
AsuC2I CCSGG 9 cut(s) 378, 392, 488, 513, 514, 534, 535, 717, 744
AvaI CYCGRG 3 cut(s) 279, 512, 533
AvaII GGWCC 7 cut(s) 314, 410, 557, 608, 665, 695, 785
AxyI CCTNAGG 1 cut(s) 267
BaeGI GKGCMC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 20, 260
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 20
BbvCI CCTCAGC 1 cut(s) 615
BbvI GCAGC 8 cut(s) 15, 88, 302, 442, 844, 856, 872, 875
BccI CCATC 2 cut(s) 541, 655
BciVI GTATCC 1 cut(s) 356
BcnI CCSGG 9 cut(s) 378, 392, 488, 513, 514, 534, 535, 717, 744
BcoDI GTCTC 2 cut(s) 282, 386
BfaI CTAG 2 cut(s) 206, 591
BfmI CTRYAG 1 cut(s) 950
BfoI RGCGCY 1 cut(s) 300
BfuI GTATCC 1 cut(s) 356
BglI GCCNNNNNGGC 2 cut(s) 308, 679
BglII AGATCT 1 cut(s) 438
Bme18I GGWCC 7 cut(s) 314, 410, 557, 608, 665, 695, 785
BmeT110I CYCGRG 3 cut(s) 279, 512, 533
BmiI GGNNCC 4 cut(s) 258, 609, 666, 696
BmsI GCATC 5 cut(s) 15, 39, 417, 510, 630
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 2 cut(s) 285, 615
BpuMI CCSGG 9 cut(s) 378, 392, 488, 513, 514, 534, 535, 717, 744
BsaHI GRCGYC 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 386
BsaWI WCCGGW 1 cut(s) 144
Bse1I ACTGG 2 cut(s) 452, 497
Bse21I CCTNAGG 1 cut(s) 267
BseAI TCCGGA 1 cut(s) 144
BseGI GGATG 2 cut(s) 54, 945
BseMII CTCAG 4 cut(s) 281, 596, 629, 836
BseNI ACTGG 2 cut(s) 452, 497
BseRI GAGGAG 5 cut(s) 152, 197, 209, 255, 348
BseSI GKGCMC 1 cut(s) 260
BseX3I CGGCCG 1 cut(s) 489
BseXI GCAGC 8 cut(s) 15, 88, 302, 442, 844, 856, 872, 875
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 953
Bsh1236I CGCG 6 cut(s) 189, 225, 330, 399, 445, 470
Bsh1285I CGRYCG 3 cut(s) 106, 196, 492
BsiEI CGRYCG 3 cut(s) 106, 196, 492
BsiHKAI GWGCWC 1 cut(s) 20
BsiHKCI CYCGRG 3 cut(s) 279, 512, 533
BslFI GGGAC 2 cut(s) 678, 726
BsmAI GTCTC 2 cut(s) 282, 386
BsmFI GGGAC 2 cut(s) 678, 726
BsmI GAATGC 5 cut(s) 726, 753, 777, 807, 887
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 3 cut(s) 279, 512, 533
Bsp120I GGGCCC 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 20, 260
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 3 cut(s) 60, 103, 438
BspCNI CTCAG 4 cut(s) 280, 595, 628, 835
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 6 cut(s) 189, 225, 330, 399, 445, 470
BspLI GGNNCC 4 cut(s) 258, 609, 666, 696
BspPI GGATC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 494
BsrI ACTGG 2 cut(s) 452, 497
BssMI GATC 3 cut(s) 60, 103, 438
BssNI GRCGYC 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 250, 852
Bst6I CTCTTC 2 cut(s) 225, 267
BstACI GRCGYC 1 cut(s) 402
BstC8I GCNNGC 5 cut(s) 260, 295, 449, 632, 726
BstDEI CTNAG 5 cut(s) 267, 285, 582, 615, 822
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 54, 945
BstFNI CGCG 6 cut(s) 189, 225, 330, 399, 445, 470
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 5 cut(s) 189, 299, 332, 447, 472
BstKTI GATC 3 cut(s) 63, 106, 441
BstMAI GTCTC 2 cut(s) 282, 386
BstMBI GATC 3 cut(s) 60, 103, 438
BstMCI CGRYCG 3 cut(s) 106, 196, 492
BstSFI CTRYAG 1 cut(s) 950
BstSLI GKGCMC 1 cut(s) 260
BstUI CGCG 6 cut(s) 189, 225, 330, 399, 445, 470
BstV1I GCAGC 8 cut(s) 15, 88, 302, 442, 844, 856, 872, 875
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BstZI CGGCCG 1 cut(s) 489
Bsu36I CCTNAGG 1 cut(s) 267
BsuI GTATCC 1 cut(s) 356
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 54, 945
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 3 cut(s) 158, 351, 504
Cac8I GCNNGC 5 cut(s) 260, 295, 449, 632, 726
CfoI GCGC 5 cut(s) 189, 299, 332, 447, 472
Cfr42I CCGCGG 1 cut(s) 226
Cfr9I CCCGGG 2 cut(s) 512, 533
CseI GACGC 1 cut(s) 391
Csp6I GTAC 2 cut(s) 215, 484
CviAII CATG 2 cut(s) 94, 112
CviQI GTAC 2 cut(s) 215, 484
DdeI CTNAG 5 cut(s) 267, 285, 582, 615, 822
DpnI GATC 3 cut(s) 62, 105, 440
DpnII GATC 3 cut(s) 60, 103, 438
DrdI GACNNNNNNGTC 1 cut(s) 792
DseDI GACNNNNNNGTC 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 489
EagI CGGCCG 1 cut(s) 489
Eam1104I CTCTTC 2 cut(s) 225, 267
EarI CTCTTC 2 cut(s) 225, 267
EciI GGCGGA 2 cut(s) 564, 660
Ecl136II GAGCTC 1 cut(s) 18
EclXI CGGCCG 1 cut(s) 489
Eco147I AGGCCT 2 cut(s) 210, 595
Eco24I GRGCYC 2 cut(s) 20, 260
Eco31I GGTCTC 1 cut(s) 386
Eco32I GATATC 1 cut(s) 530
Eco47I GGWCC 7 cut(s) 314, 410, 557, 608, 665, 695, 785
Eco52I CGGCCG 1 cut(s) 489
Eco53kI GAGCTC 1 cut(s) 18
Eco81I CCTNAGG 1 cut(s) 267
Eco88I CYCGRG 3 cut(s) 279, 512, 533
EcoICRI GAGCTC 1 cut(s) 18
EcoO109I RGGNCCY 2 cut(s) 256, 665
EcoRV GATATC 1 cut(s) 530
EcoT22I ATGCAT 1 cut(s) 777
EcoT38I GRGCYC 2 cut(s) 20, 260
FaeI CATG 2 cut(s) 97, 115
FaiI YATR 8 cut(s) 38, 95, 113, 425, 479, 777, 779, 898
FaqI GGGAC 2 cut(s) 678, 726
FatI CATG 2 cut(s) 93, 111
FauI CCCGC 3 cut(s) 267, 456, 639
FauNDI CATATG 1 cut(s) 777
FokI GGATG 2 cut(s) 61, 932
FriOI GRGCYC 2 cut(s) 20, 260
FspBI CTAG 2 cut(s) 206, 591
GlaI GCGC 5 cut(s) 188, 298, 331, 446, 471
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 300
HgaI GACGC 1 cut(s) 391
HhaI GCGC 5 cut(s) 189, 299, 332, 447, 472
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 2 cut(s) 97, 115
Hin6I GCGC 5 cut(s) 187, 297, 330, 445, 470
HinP1I GCGC 5 cut(s) 187, 297, 330, 445, 470
HinfI GANTC 2 cut(s) 275, 502
Hpy166II GTNNAC 5 cut(s) 410, 509, 560, 611, 698
Hpy188I TCNGA 4 cut(s) 48, 459, 546, 825
Hpy188III TCNNGA 3 cut(s) 145, 269, 279
Hpy8I GTNNAC 5 cut(s) 410, 509, 560, 611, 698
Hpy99I CGWCG 4 cut(s) 86, 194, 254, 407
HpyAV CCTTC 2 cut(s) 109, 949
HpyCH4III ACNGT 2 cut(s) 250, 852
HpyCH4V TGCA 7 cut(s) 31, 430, 643, 724, 775, 896, 934
HpyF3I CTNAG 5 cut(s) 267, 285, 582, 615, 822
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 2 cut(s) 97, 115
HspAI GCGC 5 cut(s) 187, 297, 330, 445, 470
Kpn2I TCCGGA 1 cut(s) 144
KspI CCGCGG 1 cut(s) 226
Kzo9I GATC 3 cut(s) 60, 103, 438
LmnI GCTCC 5 cut(s) 23, 184, 361, 499, 733
Lsp1109I GCAGC 8 cut(s) 15, 88, 302, 442, 844, 856, 872, 875
LweI GCATC 5 cut(s) 15, 39, 417, 510, 630
MaeI CTAG 2 cut(s) 206, 591
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 62, 105, 440
MbiI CCGCTC 1 cut(s) 494
MboI GATC 3 cut(s) 60, 103, 438
MboII GAAGA 3 cut(s) 184, 242, 284
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 20, 260
MluCI AATT 2 cut(s) 32, 620
MlyI GAGTC 1 cut(s) 284
Mph1103I ATGCAT 1 cut(s) 777
MroI TCCGGA 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 225
Mva1269I GAATGC 5 cut(s) 726, 753, 777, 807, 887
MvnI CGCG 6 cut(s) 189, 225, 330, 399, 445, 470
NciI CCSGG 9 cut(s) 378, 392, 488, 513, 514, 534, 535, 717, 744
NdeI CATATG 1 cut(s) 777
NdeII GATC 3 cut(s) 60, 103, 438
NlaIII CATG 2 cut(s) 97, 115
NlaIV GGNNCC 4 cut(s) 258, 609, 666, 696
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 1 cut(s) 55
NsiI ATGCAT 1 cut(s) 777
PaeR7I CTCGAG 1 cut(s) 279
PasI CCCWGGG 1 cut(s) 677
PceI AGGCCT 2 cut(s) 210, 595
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 132, 186
PctI GAATGC 5 cut(s) 726, 753, 777, 807, 887
PfeI GAWTC 1 cut(s) 502
PflMI CCANNNNNTGG 1 cut(s) 566
PfoI TCCNGGA 3 cut(s) 316, 742, 796
Ple19I CGATCG 1 cut(s) 106
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 665
Psp124BI GAGCTC 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 665
PspFI CCCAGC 1 cut(s) 372
PspN4I GGNNCC 4 cut(s) 258, 609, 666, 696
PspOMI GGGCCC 1 cut(s) 256
PspPPI RGGWCCY 1 cut(s) 665
PsuI RGATCY 1 cut(s) 438
PvuI CGATCG 1 cut(s) 106
RsaI GTAC 2 cut(s) 216, 485
RsaNI GTAC 2 cut(s) 215, 484
SacI GAGCTC 1 cut(s) 20
SacII CCGCGG 1 cut(s) 226
Sau3AI GATC 3 cut(s) 60, 103, 438
SchI GAGTC 1 cut(s) 284
SduI GDGCHC 2 cut(s) 20, 260
SfaNI GCATC 5 cut(s) 15, 39, 417, 510, 630
SfcI CTRYAG 1 cut(s) 950
Sfr274I CTCGAG 1 cut(s) 279
Sfr303I CCGCGG 1 cut(s) 226
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 7 cut(s) 314, 410, 557, 608, 665, 695, 785
SlaI CTCGAG 1 cut(s) 279
SmaI CCCGGG 2 cut(s) 514, 535
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 2 cut(s) 32, 620
SseBI AGGCCT 2 cut(s) 210, 595
SspMI CTAG 2 cut(s) 206, 591
SstI GAGCTC 1 cut(s) 20
StuI AGGCCT 2 cut(s) 210, 595
TaaI ACNGT 2 cut(s) 250, 852
TaqI TCGA 4 cut(s) 5, 102, 252, 280
TasI AATT 2 cut(s) 32, 620
TfiI GAWTC 1 cut(s) 502
TscAI CASTG 3 cut(s) 165, 358, 504
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 8 cut(s) 28, 76, 290, 430, 832, 844, 885, 888
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 3 cut(s) 25, 82, 128
TspMI CCCGGG 2 cut(s) 512, 533
TspRI CASTG 3 cut(s) 165, 358, 504
Van91I CCANNNNNTGG 1 cut(s) 566
VpaK11BI GGWCC 7 cut(s) 314, 410, 557, 608, 665, 695, 785
XhoI CTCGAG 1 cut(s) 279
XmaI CCCGGG 2 cut(s) 512, 533
XspI CTAG 2 cut(s) 206, 591
Zsp2I ATGCAT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.