MD04G1223300.v1.1

Small nuclear ribonucleoprotein-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001710
Physical Location & Seq
Forward (+)
30359494 .. 30359865
372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1223300.v1.1.491

Sequence Viewer

Length: 372 bp
ATGTTGATGTCGAAGAGCTCGAAGATGCTGCAATTCGTAAACTACCGCATGCGGGTCACGATCCAGGACGGTCGCCAGCTCGTCGGAAACTTCATGGCCTTCGACCGCCACATGAACCTTGTCCTCGGCAACTGTGAGGAGTTCCGCAATCTCCCCCTTCTCAAGGGCAACAAGAAGGAGGACTGGGATGACAGCCGCACCTTCGGCCTCGTCCTCCTCCGCGGCGAGGAGGTCGTCTCCATGATCGTCGAGGGCCCGCCTCCTCAGGAAGAATCCTGCACCAAGGCTACCAGCGCCGCCCCGTTGGCCGATCCCAGTATAGGCCGCGTCGCTGGGAGAGGTATTCCTACAACCCCTCTCGCCCAAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05322 map03040 map05322
Pfam Domains
Protein Families
LSM

Protein Analysis

124

Amino Acids

13.62

Weight (kDa)

7.79

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 9 - 82 3.1e-16 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 222, 327
AclWI GGATC 2 cut(s) 55, 305
AcoI YGGCCR 1 cut(s) 306
AfiI CCNNNNNNNGG 4 cut(s) 52, 163, 226, 320
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 2 cut(s) 18, 79
AluI AGCT 2 cut(s) 18, 79
Alw21I GWGCWC 1 cut(s) 20
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 2 cut(s) 55, 305
AoxI GGCC 5 cut(s) 96, 205, 253, 306, 322
ApaI GGGCCC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 28
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 2 cut(s) 253, 254
AxyI CCTNAGG 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 257
BanII GRGCYC 2 cut(s) 20, 257
Bbv12I GWGCWC 1 cut(s) 20
BbvI GCAGC 1 cut(s) 15
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 297
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 5 cut(s) 29, 196, 223, 297, 325
BlsI GCNGC 5 cut(s) 30, 197, 224, 298, 326
Bme1390I CCNGG 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 253, 254
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 65
BmrI ACTGGG 2 cut(s) 193, 309
BmsI GCATC 1 cut(s) 15
BmuI ACTGGG 2 cut(s) 193, 309
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpuEI CTTGAG 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 124, 220, 282
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 163, 226, 320
Bse1I ACTGG 2 cut(s) 188, 315
Bse21I CCTNAGG 1 cut(s) 264
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 3 cut(s) 124, 220, 282
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 4 cut(s) 52, 163, 226, 320
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 2 cut(s) 188, 315
BseRI GAGGAG 4 cut(s) 152, 206, 242, 252
BseSI GKGCMC 1 cut(s) 257
BseXI GCAGC 1 cut(s) 15
BseYI CCCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 2 cut(s) 222, 327
Bsh1285I CGRYCG 2 cut(s) 73, 106
BshFI GGCC 5 cut(s) 98, 207, 255, 308, 324
BsiEI CGRYCG 2 cut(s) 73, 106
BsiHKAI GWGCWC 1 cut(s) 20
BslI CCNNNNNNNGG 4 cut(s) 52, 163, 226, 320
BsmAI GTCTC 1 cut(s) 241
BsmBI CGTCTC 1 cut(s) 241
BsnI GGCC 5 cut(s) 98, 207, 255, 308, 324
Bsp120I GGGCCC 1 cut(s) 253
Bsp1286I GDGCHC 2 cut(s) 20, 257
Bsp143I GATC 3 cut(s) 60, 243, 310
BspANI GGCC 5 cut(s) 98, 207, 255, 308, 324
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 2 cut(s) 222, 327
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 2 cut(s) 55, 305
BspQI GCTCTTC 1 cut(s) 8
BsrI ACTGG 2 cut(s) 188, 315
BssECI CCNNGG 3 cut(s) 124, 220, 282
BssMI GATC 3 cut(s) 60, 243, 310
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 2 cut(s) 71, 134
Bst6I CTCTTC 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 50, 77, 257
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 1 cut(s) 220
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 1 cut(s) 193
BstFNI CGCG 2 cut(s) 222, 327
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 63, 246, 313
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 3 cut(s) 60, 243, 310
BstMCI CGRYCG 2 cut(s) 73, 106
BstMWI GCNNNNNNNGC 3 cut(s) 204, 293, 305
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 257
BstUI CGCG 2 cut(s) 222, 327
BstV1I GCAGC 1 cut(s) 15
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 5 cut(s) 98, 207, 255, 308, 324
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 3 cut(s) 50, 77, 257
CfoI GCGC 1 cut(s) 296
Cfr13I GGNCC 2 cut(s) 253, 254
Cfr42I CCGCGG 1 cut(s) 223
CseI GACGC 1 cut(s) 316
CviAII CATG 4 cut(s) 49, 94, 112, 241
CviJI RGCY 9 cut(s) 18, 79, 98, 195, 207, 255, 287, 308, 324
CviKI_1 RGCY 9 cut(s) 18, 79, 98, 195, 207, 255, 287, 308, 324
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 62, 245, 312
DpnII GATC 3 cut(s) 60, 243, 310
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 2 cut(s) 20, 257
Eco53kI GAGCTC 1 cut(s) 18
Eco81I CCTNAGG 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 18
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 2 cut(s) 20, 257
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 4 cut(s) 52, 97, 115, 244
FaiI YATR 5 cut(s) 50, 95, 113, 242, 320
FatI CATG 4 cut(s) 48, 93, 111, 240
FauI CCCGC 2 cut(s) 45, 264
Fnu4HI GCNGC 5 cut(s) 29, 196, 223, 297, 325
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 2 cut(s) 20, 257
Fsp4HI GCNGC 5 cut(s) 29, 196, 223, 297, 325
GlaI GCGC 1 cut(s) 295
GluI GCNGC 5 cut(s) 29, 196, 223, 297, 325
GsaI CCCAGC 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 5 cut(s) 98, 207, 255, 308, 324
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 296
Hin1II CATG 4 cut(s) 52, 97, 115, 244
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 2 cut(s) 58, 266
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 3 cut(s) 86, 251, 332
HpyAV CCTTC 4 cut(s) 109, 167, 169, 211
HpyCH4III ACNGT 2 cut(s) 71, 134
HpyCH4V TGCA 2 cut(s) 31, 279
HpyF10VI GCNNNNNNNGC 3 cut(s) 204, 293, 305
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 4 cut(s) 52, 97, 115, 244
HspAI GCGC 1 cut(s) 294
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 3 cut(s) 60, 243, 310
LguI GCTCTTC 1 cut(s) 8
LpnPI CCDG 9 cut(s) 50, 77, 89, 169, 251, 289, 304, 318, 328
Lsp1109I GCAGC 1 cut(s) 15
LweI GCATC 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 62, 245, 312
MboI GATC 3 cut(s) 60, 243, 310
MboII GAAGA 3 cut(s) 25, 34, 281
MhlI GDGCHC 2 cut(s) 20, 257
MluCI AATT 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 64
MspA1I CMGCKG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 222, 327
MwoI GCNNNNNNNGC 3 cut(s) 204, 293, 305
NdeII GATC 3 cut(s) 60, 243, 310
NlaIII CATG 4 cut(s) 52, 97, 115, 244
NlaIV GGNNCC 1 cut(s) 255
NmeAIII GCCGAG 1 cut(s) 105
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 52
PaeI GCATGC 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PfeI GAWTC 1 cut(s) 272
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 5 cut(s) 30, 197, 224, 298, 326
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 255
PspOMI GGGCCC 1 cut(s) 253
PspPI GGNCC 2 cut(s) 253, 254
SacI GAGCTC 1 cut(s) 20
SacII CCGCGG 1 cut(s) 223
SapI GCTCTTC 1 cut(s) 8
SatI GCNGC 5 cut(s) 29, 196, 223, 297, 325
Sau3AI GATC 3 cut(s) 60, 243, 310
Sau96I GGNCC 2 cut(s) 253, 254
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 2 cut(s) 20, 257
SetI ASST 6 cut(s) 20, 81, 120, 203, 234, 343
SfaNI GCATC 1 cut(s) 15
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SphI GCATGC 1 cut(s) 52
Sse9I AATT 1 cut(s) 32
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 71, 134
TaqI TCGA 4 cut(s) 11, 20, 102, 249
TasI AATT 1 cut(s) 32
TauI GCSGC 4 cut(s) 198, 225, 299, 327
TfiI GAWTC 1 cut(s) 272
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 82, 128
XagI CCTNNNNNAGG 1 cut(s) 161
XceI RCATGY 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.