Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002204

Physical Location & Seq

Genomic Coordinates Forward (+)

13844059 .. 13846746

Gene Structure 2688 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G27760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGAGTGACGAAACGACGTCATCTCCGTCCCCAGCTCCGGCGAAGAAGAAGCAGAATCTGGGATGGATGGAGTGGATGAGGGGATGGAGCAGTGTTTTCGGGGAGATTCTCTTCCAGAGGATCACAGCTTCTCATTTGGAGAATCCTCTTCCTCTTCCTTCCGTCAACGACCTCACTTGCGTTGTCACTGGCTCCACCAGCGGCATTGGCCGTGAAACCGCGAGGCAGCTTGCAGAAGCTGGTGCTCATGTTGTGATGGCCGTAAGGAACACAAAGGCGGCTCAGGAGCTGATACTGCAATGGCAGAACGAATGGTCTGGTAAAGGTCTCCCACTCAATATTGAGGCAATGGAGATTGATCTACTCTCACTGGATTCTGTCGCGAGATTTGCTGAGGCTTTCAACGCTCGGTTAGGACCTTTGCATGTTCTGATTAACAATGCTGGGATGTTTGCTATGGGAGAGGCGCAAAAATTCTCAGAGGAAGGATATGAGCAGCACATGCAAGTGAATCATTTAGCTCCAGCGCTGCTTTCAGTACTTCTTTTGCCGTCTCTGATCCGAGGCTCTCCTAGCCGAATCATTAATGTGAATTCCGTTATGCATAGTGTCGGTTTTGTTGACCCGGATGACATGAATGTTGTTTCTGGTAGACGTAAGTACTCAAGCCTTATAGGATACTCAAGCAGCAAGCTTGCCCAGATTATGTTTAGTAGCATTCTTTTCAAAAAGCTTCCTCTGGAAACAGGAGTCAGCGTCGTATGTCTATCCCCTGGTGTTGTCCTAACAAATGTTGCCAGGGATCTATCCAGGATTCTTCAAGCTCTTTACGCAGTGATACCTTATTTCATATTTTCACCCCAAGAAGGTTGTAGAAGTTCTCTATTCTCGGCCACAGATCCTCAGATTCCAGAGTACTGGGAAACACTAAAAAACGATGATTGGCCTGTTTGCCCATTCATCTCTCAAGATTGCCGCCCTGCAAATCCTTCCGAAGAAGCACACAACACAGAAACTGCACAGAGAGTGTGGAAAAAGACGTTAGAGCTGGTGGGTCTTCCTCTCGATGCAGTTGAGAAGCTCATAGAAGGGGAAAATATCCAATGCCGGTATGGAGCACAACACGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001523 GO:0003674 GO:0003824 GO:0004033 GO:0004745 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006066 GO:0006081 GO:0006629 GO:0006720 GO:0006721 GO:0008106 GO:0008150 GO:0008152 GO:0009888 GO:0009987 GO:0010073 GO:0010817 GO:0012505 GO:0016020 GO:0016101 GO:0016491 GO:0016614 GO:0016616 GO:0031984 GO:0032501 GO:0032502 GO:0034308 GO:0034754 GO:0042175 GO:0042445 GO:0042572 GO:0042574 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0052650 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901615 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

adh_short

Protein Analysis

376

Amino Acids

41.48

Weight (kDa)

5.48

Isoelectric Point (pI)

49.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KR	PF08659	59 - 170	6.5e-10	KR domain
adh_short	PF00106	60 - 205	7e-28	short chain dehydrogenase
adh_short_C2	PF13561	66 - 214	5.2e-18	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27760 AT5G53090 AT5G53100
fragaria_vesca	FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31671
malus_domestica	MD02G1050600.v1.1 MD02G1208700.v1.1 MD06G1238500.v1.1 MD14G1244800.v1.1 MD16G1170300.v1.1
prunus_persica	Prupe.5G242400_v2.0.a1
pyrus_communis	pycom06g21400 pycom14g20600
rosa_chinensis	RchiOBHm_Chr6g0266921 RchiOBHm_Chr7g0227471
rosa_laevigata	RLG00000001643 RLG00000014057
rosa_multiflora	Rmu_sc0001772.1_g000037 Rmu_sc0001772.1_g000038 Rmu_ssc0000486.1_g000008
rosa_roxburghii	Rroxscaffold_3G00232120 Rroxscaffold_7G00201290
rosa_rugosa	Rorug06G0028300 Rorug06G0028400
rosa_samantha	Rh6AG149400 Rh6BG150300 Rh6CG145200 Rh6DG134500 Rh7AG394300 Rh7CG413400 Rh7DG390500
rosa_wichuraiana	Rw6G012920 Rw7G032830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	20
AccI	GTMKAC	1 cut(s)	652
AccII	CGCG	2 cut(s)	221, 383
AciI	CCGC	4 cut(s)	201, 219, 278, 976
AclWI	GGATC	4 cut(s)	128, 553, 810, 893
AcoI	YGGCCR	3 cut(s)	208, 258, 891
AcsI	RAATTY	2 cut(s)	473, 592
AcyI	GRCGYC	1 cut(s)	17
AfaI	GTAC	3 cut(s)	540, 662, 917
AfeI	AGCGCT	1 cut(s)	528
AfiI	CCNNNNNNNGG	2 cut(s)	37, 866
AgsI	TTSAA	3 cut(s)	403, 727, 821
AjnI	CCWGG	3 cut(s)	772, 797, 809
Alw21I	GWGCWC	2 cut(s)	247, 1120
Alw26I	GTCTC	2 cut(s)	332, 558
AlwI	GGATC	4 cut(s)	128, 553, 810, 893
AlwNI	CAGNNNCTG	4 cut(s)	58, 239, 289, 1016
Aor51HI	AGCGCT	1 cut(s)	528
AoxI	GGCC	4 cut(s)	208, 258, 891, 944
ApeKI	GCWGC	4 cut(s)	226, 496, 529, 687
ApoI	RAATTY	2 cut(s)	473, 592
AseI	ATTAAT	1 cut(s)	585
AspLEI	GCGC	2 cut(s)	469, 529
AspS9I	GGNCC	1 cut(s)	416
AsuC2I	CCSGG	1 cut(s)	626
AsuHPI	GGTGA	1 cut(s)	849
AvaII	GGWCC	1 cut(s)	416
BbsI	GAAGAC	1 cut(s)	1049
Bbv12I	GWGCWC	2 cut(s)	247, 1120
BbvCI	CCTCAGC	1 cut(s)	393
BbvI	GCAGC	4 cut(s)	238, 508, 516, 699
BccI	CCATC	4 cut(s)	57, 61, 78, 250
BceAI	ACGGC	3 cut(s)	195, 245, 535
BcgI	CGANNNNNNTGC	2 cut(s)	79, 113
BciT130I	CCWGG	3 cut(s)	774, 799, 811
BciVI	GTATCC	1 cut(s)	671
BcnI	CCSGG	1 cut(s)	626
BcoDI	GTCTC	2 cut(s)	332, 558
BfaI	CTAG	1 cut(s)	573
BfoI	RGCGCY	1 cut(s)	530
BfuI	GTATCC	1 cut(s)	671
BisI	GCNGC	7 cut(s)	202, 227, 279, 497, 530, 688, 976
BlsI	GCNGC	7 cut(s)	203, 228, 280, 498, 531, 689, 977
BmcAI	AGTACT	3 cut(s)	540, 662, 917
Bme1390I	CCNGG	4 cut(s)	626, 774, 799, 811
Bme18I	GGWCC	1 cut(s)	416
BmgT120I	GGNCC	1 cut(s)	416
BmiI	GGNNCC	1 cut(s)	193
BmrFI	CCNGG	4 cut(s)	626, 774, 799, 811
BmrI	ACTGGG	1 cut(s)	928
BmsI	GCATC	1 cut(s)	1057
BmuI	ACTGGG	1 cut(s)	928
BpiI	GAAGAC	1 cut(s)	1049
BpmI	CTGGAG	1 cut(s)	507
Bpu10I	CCTNAGC	2 cut(s)	282, 393
BpuEI	CTTGAG	3 cut(s)	649, 667, 951
BpuMI	CCSGG	1 cut(s)	626
BsaHI	GRCGYC	1 cut(s)	17
BsaI	GGTCTC	1 cut(s)	332
BsaJI	CCNNGG	3 cut(s)	562, 772, 798
BsaXI	ACNNNNNCTCC	4 cut(s)	7, 37, 741, 771
Bsc4I	CCNNNNNNNGG	2 cut(s)	37, 866
Bse118I	RCCGGY	1 cut(s)	1107
Bse1I	ACTGG	3 cut(s)	193, 375, 923
Bse3DI	GCAATG	2 cut(s)	305, 354
BseBI	CCWGG	3 cut(s)	774, 799, 811
BseDI	CCNNGG	3 cut(s)	562, 772, 798
BseGI	GGATG	6 cut(s)	68, 72, 81, 89, 453, 634
BseLI	CCNNNNNNNGG	2 cut(s)	37, 866
BseMI	GCAATG	2 cut(s)	305, 354
BseMII	CTCAG	4 cut(s)	296, 384, 492, 917
BseNI	ACTGG	3 cut(s)	193, 375, 923
BseXI	GCAGC	4 cut(s)	238, 508, 516, 699
BseYI	CCCAGC	2 cut(s)	31, 443
BsgI	GTGCAG	1 cut(s)	1002
Bsh1236I	CGCG	2 cut(s)	221, 383
BshFI	GGCC	4 cut(s)	210, 260, 893, 946
BsiHKAI	GWGCWC	2 cut(s)	247, 1120
BsiSI	CCGG	3 cut(s)	38, 626, 1108
BslFI	GGGAC	1 cut(s)	13
BslI	CCNNNNNNNGG	2 cut(s)	37, 866
BsmAI	GTCTC	2 cut(s)	332, 558
BsmBI	CGTCTC	1 cut(s)	558
BsmFI	GGGAC	1 cut(s)	13
BsmI	GAATGC	1 cut(s)	717
BsnI	GGCC	4 cut(s)	210, 260, 893, 946
Bso31I	GGTCTC	1 cut(s)	332
Bsp1286I	GDGCHC	2 cut(s)	247, 1120
Bsp143I	GATC	5 cut(s)	120, 358, 558, 802, 898
Bsp68I	TCGCGA	1 cut(s)	383
BspACI	CCGC	4 cut(s)	201, 219, 278, 976
BspANI	GGCC	4 cut(s)	210, 260, 893, 946
BspCNI	CTCAG	4 cut(s)	295, 385, 491, 916
BspFNI	CGCG	2 cut(s)	221, 383
BspLI	GGNNCC	1 cut(s)	193
BspPI	GGATC	4 cut(s)	128, 553, 810, 893
BspTNI	GGTCTC	1 cut(s)	332
BsrDI	GCAATG	2 cut(s)	305, 354
BsrFI	RCCGGY	1 cut(s)	1107
BsrI	ACTGG	3 cut(s)	193, 375, 923
BssAI	RCCGGY	1 cut(s)	1107
BssECI	CCNNGG	3 cut(s)	562, 772, 798
BssMI	GATC	5 cut(s)	120, 358, 558, 802, 898
BssNI	GRCGYC	1 cut(s)	17
Bst2UI	CCWGG	3 cut(s)	774, 799, 811
Bst6I	CTCTTC	3 cut(s)	116, 153, 159
BstACI	GRCGYC	1 cut(s)	17
BstAPI	GCANNNNNTGC	1 cut(s)	502
BstC8I	GCNNGC	3 cut(s)	231, 692, 696
BstDEI	CTNAG	4 cut(s)	282, 393, 478, 903
BstF5I	GGATG	6 cut(s)	68, 72, 81, 89, 453, 634
BstFNI	CGCG	2 cut(s)	221, 383
BstH2I	RGCGCY	1 cut(s)	530
BstHHI	GCGC	2 cut(s)	469, 529
BstKTI	GATC	5 cut(s)	123, 361, 561, 805, 901
BstMAI	GTCTC	2 cut(s)	332, 558
BstMBI	GATC	5 cut(s)	120, 358, 558, 802, 898
BstMWI	GCNNNNNNNGC	8 cut(s)	198, 207, 295, 389, 404, 502, 573, 830
BstNI	CCWGG	3 cut(s)	774, 799, 811
BstNSI	RCATGY	2 cut(s)	428, 505
BstSCI	CCNGG	4 cut(s)	624, 772, 797, 809
BstUI	CGCG	2 cut(s)	221, 383
BstV1I	GCAGC	4 cut(s)	238, 508, 516, 699
BstV2I	GAAGAC	1 cut(s)	1049
BstX2I	RGATCY	2 cut(s)	802, 898
BstXI	CCANNNNNNTGG	1 cut(s)	918
BstYI	RGATCY	2 cut(s)	802, 898
BsuI	GTATCC	1 cut(s)	671
BsuRI	GGCC	4 cut(s)	210, 260, 893, 946
BtsCI	GGATG	6 cut(s)	68, 72, 81, 89, 453, 634
BtsI	GCAGTG	2 cut(s)	97, 840
BtsIMutI	CAGTG	4 cut(s)	97, 186, 368, 840
BtuMI	TCGCGA	1 cut(s)	383
Cac8I	GCNNGC	3 cut(s)	231, 692, 696
CaiI	CAGNNNCTG	4 cut(s)	58, 239, 289, 1016
CfoI	GCGC	2 cut(s)	469, 529
Cfr10I	RCCGGY	1 cut(s)	1107
Cfr13I	GGNCC	1 cut(s)	416
CseI	GACGC	1 cut(s)	745
Csp6I	GTAC	3 cut(s)	539, 661, 916
CviAII	CATG	4 cut(s)	248, 425, 502, 634
CviQI	GTAC	3 cut(s)	539, 661, 916
DdeI	CTNAG	4 cut(s)	282, 393, 478, 903
DpnI	GATC	5 cut(s)	122, 360, 560, 804, 900
DpnII	GATC	5 cut(s)	120, 358, 558, 802, 898
EaeI	YGGCCR	3 cut(s)	208, 258, 891
Eam1104I	CTCTTC	3 cut(s)	116, 153, 159
EarI	CTCTTC	3 cut(s)	116, 153, 159
Eco31I	GGTCTC	1 cut(s)	332
Eco47I	GGWCC	1 cut(s)	416
Eco47III	AGCGCT	1 cut(s)	528
EcoO109I	RGGNCCY	1 cut(s)	416
EcoRI	GAATTC	1 cut(s)	592
EcoRII	CCWGG	3 cut(s)	772, 797, 809
EcoT22I	ATGCAT	1 cut(s)	606
Esp3I	CGTCTC	1 cut(s)	558
FaeI	CATG	4 cut(s)	251, 428, 505, 637
FaqI	GGGAC	1 cut(s)	13
FatI	CATG	4 cut(s)	247, 424, 501, 633
FblI	GTMKAC	1 cut(s)	652
Fnu4HI	GCNGC	7 cut(s)	202, 227, 279, 497, 530, 688, 976
FokI	GGATG	6 cut(s)	75, 79, 88, 96, 460, 641
Fsp4HI	GCNGC	7 cut(s)	202, 227, 279, 497, 530, 688, 976
FspBI	CTAG	1 cut(s)	573
GlaI	GCGC	2 cut(s)	468, 528
GluI	GCNGC	7 cut(s)	202, 227, 279, 497, 530, 688, 976
GsaI	CCCAGC	2 cut(s)	35, 447
GsuI	CTGGAG	1 cut(s)	507
HaeII	RGCGCY	1 cut(s)	530
HaeIII	GGCC	4 cut(s)	210, 260, 893, 946
HapII	CCGG	3 cut(s)	38, 626, 1108
HgaI	GACGC	1 cut(s)	745
HhaI	GCGC	2 cut(s)	469, 529
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	4 cut(s)	251, 428, 505, 637
Hin6I	GCGC	2 cut(s)	467, 527
HinP1I	GCGC	2 cut(s)	467, 527
HincII	GTYRAC	2 cut(s)	166, 622
HindII	GTYRAC	2 cut(s)	166, 622
HindIII	AAGCTT	2 cut(s)	692, 731
HinfI	GANTC	9 cut(s)	55, 106, 142, 374, 511, 579, 750, 814, 907
HpaII	CCGG	3 cut(s)	38, 626, 1108
HphI	GGTGA	1 cut(s)	849
Hpy166II	GTNNAC	3 cut(s)	166, 622, 653
Hpy188I	TCNGA	6 cut(s)	432, 481, 558, 563, 906, 994
Hpy188III	TCNNGA	7 cut(s)	115, 284, 382, 740, 911, 968, 1064
Hpy8I	GTNNAC	3 cut(s)	166, 622, 653
Hpy99I	CGWCG	2 cut(s)	19, 761
HpyAV	CCTTC	5 cut(s)	168, 479, 860, 999, 1082
HpyCH4IV	ACGT	3 cut(s)	17, 655, 1040
HpyCH4V	TGCA	8 cut(s)	233, 298, 424, 505, 604, 983, 1019, 1070
HpyF10VI	GCNNNNNNNGC	8 cut(s)	198, 207, 295, 389, 404, 502, 573, 830
HpyF3I	CTNAG	4 cut(s)	282, 393, 478, 903
HpySE526I	ACGT	3 cut(s)	17, 655, 1040
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	4 cut(s)	251, 428, 505, 637
HspAI	GCGC	2 cut(s)	467, 527
Kzo9I	GATC	5 cut(s)	120, 358, 558, 802, 898
LmnI	GCTCC	6 cut(s)	40, 87, 197, 286, 526, 1115
Lsp1109I	GCAGC	4 cut(s)	238, 508, 516, 699
LweI	GCATC	1 cut(s)	1057
MaeI	CTAG	1 cut(s)	573
MaeII	ACGT	3 cut(s)	17, 655, 1040
MaeIII	GTNAC	2 cut(s)	5, 184
MalI	GATC	5 cut(s)	122, 360, 560, 804, 900
MboI	GATC	5 cut(s)	120, 358, 558, 802, 898
MboII	GAAGA	8 cut(s)	55, 58, 103, 140, 146, 809, 1007, 1049
MflI	RGATCY	2 cut(s)	802, 898
MhlI	GDGCHC	2 cut(s)	247, 1120
MluCI	AATT	2 cut(s)	473, 592
MlyI	GAGTC	1 cut(s)	759
Mph1103I	ATGCAT	1 cut(s)	606
MseI	TTAA	2 cut(s)	435, 585
MslI	CAYNNNNRTG	2 cut(s)	506, 587
MspA1I	CMGCKG	1 cut(s)	201
MspI	CCGG	3 cut(s)	38, 626, 1108
MspR9I	CCNGG	4 cut(s)	626, 774, 799, 811
Mva1269I	GAATGC	1 cut(s)	717
MvaI	CCWGG	3 cut(s)	774, 799, 811
MvnI	CGCG	2 cut(s)	221, 383
MwoI	GCNNNNNNNGC	8 cut(s)	198, 207, 295, 389, 404, 502, 573, 830
NciI	CCSGG	1 cut(s)	626
NdeII	GATC	5 cut(s)	120, 358, 558, 802, 898
NlaIII	CATG	4 cut(s)	251, 428, 505, 637
NlaIV	GGNNCC	1 cut(s)	193
NmeAIII	GCCGAG	1 cut(s)	869
NmuCI	GTSAC	2 cut(s)	5, 184
NruI	TCGCGA	1 cut(s)	383
NsiI	ATGCAT	1 cut(s)	606
NspI	RCATGY	2 cut(s)	428, 505
PcsI	WCGNNNNNNNCGW	1 cut(s)	23
PctI	GAATGC	1 cut(s)	717
PfeI	GAWTC	8 cut(s)	55, 106, 142, 374, 511, 579, 814, 907
PfoI	TCCNGGA	1 cut(s)	809
PkrI	GCNGC	7 cut(s)	203, 228, 280, 498, 531, 689, 977
PleI	GAGTC	1 cut(s)	758
PpsI	GAGTC	1 cut(s)	758
PpuMI	RGGWCCY	1 cut(s)	416
PshBI	ATTAAT	1 cut(s)	585
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	3 cut(s)	772, 797, 809
PspFI	CCCAGC	2 cut(s)	31, 443
PspGI	CCWGG	3 cut(s)	772, 797, 809
PspN4I	GGNNCC	1 cut(s)	193
PspPI	GGNCC	1 cut(s)	416
PspPPI	RGGWCCY	1 cut(s)	416
PstNI	CAGNNNCTG	4 cut(s)	58, 239, 289, 1016
PsuI	RGATCY	2 cut(s)	802, 898
RruI	TCGCGA	1 cut(s)	383
RsaI	GTAC	3 cut(s)	540, 662, 917
RsaNI	GTAC	3 cut(s)	539, 661, 916
RseI	CAYNNNNRTG	2 cut(s)	506, 587
SaqAI	TTAA	2 cut(s)	435, 585
SatI	GCNGC	7 cut(s)	202, 227, 279, 497, 530, 688, 976
Sau3AI	GATC	5 cut(s)	120, 358, 558, 802, 898
Sau96I	GGNCC	1 cut(s)	416
ScaI	AGTACT	3 cut(s)	540, 662, 917
SchI	GAGTC	1 cut(s)	759
ScrFI	CCNGG	4 cut(s)	626, 774, 799, 811
SduI	GDGCHC	2 cut(s)	247, 1120
SfaNI	GCATC	1 cut(s)	1057
SinI	GGWCC	1 cut(s)	416
SmiMI	CAYNNNNRTG	2 cut(s)	506, 587
SmlI	CTYRAG	3 cut(s)	664, 682, 966
SmoI	CTYRAG	3 cut(s)	664, 682, 966
Sse9I	AATT	2 cut(s)	473, 592
SsiI	CCGC	4 cut(s)	201, 219, 278, 976
SspI	AATATT	1 cut(s)	340
SspMI	CTAG	1 cut(s)	573
StyD4I	CCNGG	4 cut(s)	624, 772, 797, 809
TaiI	ACGT	3 cut(s)	20, 658, 1043
TaqI	TCGA	1 cut(s)	1065
TasI	AATT	2 cut(s)	473, 592
TatI	WGTACW	3 cut(s)	538, 660, 915
TauI	GCSGC	3 cut(s)	204, 281, 978
TfiI	GAWTC	8 cut(s)	55, 106, 142, 374, 511, 579, 814, 907
Tru1I	TTAA	2 cut(s)	435, 585
Tru9I	TTAA	2 cut(s)	435, 585
TscAI	CASTG	4 cut(s)	97, 193, 375, 840
TseFI	GTSAC	2 cut(s)	5, 184
TseI	GCWGC	4 cut(s)	226, 496, 529, 687
Tsp45I	GTSAC	2 cut(s)	5, 184
TspDTI	ATGAA	3 cut(s)	650, 838, 949
TspGWI	ACGGA	3 cut(s)	15, 151, 586
TspRI	CASTG	4 cut(s)	97, 193, 375, 840
VpaK11BI	GGWCC	1 cut(s)	416
VspI	ATTAAT	1 cut(s)	585
XapI	RAATTY	2 cut(s)	473, 592
XceI	RCATGY	2 cut(s)	428, 505
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1109
XmiI	GTMKAC	1 cut(s)	652
XspI	CTAG	1 cut(s)	573
ZraI	GACGTC	1 cut(s)	18
ZrmI	AGTACT	3 cut(s)	540, 662, 917
Zsp2I	ATGCAT	1 cut(s)	606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.