Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0002204

Physical Location & Seq

Genomic Coordinates Reverse (-)

18985953 .. 18989711

Gene Structure 3759 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG145200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGCTGTTCCAGAGAATCACTGCCAGCCATTTGATGAACCCAATGCCTTTGCCTCCTGTCAACGATCTCACATGCATTGTCACTGGATCCACTAGCGGTATCGGCCGCGAAATCGCCAGGCAGTTGGCGGAGTCTGGTGCACATGTTGTAATGGCAGTGAGAAACACCAAAGCAGCTAAGGAGCTGATCCAGAAGTGGCAGAATGAATGGTCAGGAATGGGCCTTCCTCTCAATGTTGAGGTGATGGAACTTGATCTACTCTCCTTGGATTCTGTTGTGAGATTTTCTGAAGAGTGGAATGCACGATTGGGACCTCTGCATGTTCTTATCAACAATGCTGGGATATTTTCTATTGGAGAGCCACAAAAATTTTCAAAAGATGGGTATGAAGAGCACTTGCAAGTGAATCATCTGGCTCCAGCACTGCTATCAGTATTGCTACTGCCATCCCTTATTAGAGGCTCTCCAAGTCGAATTGTTAATGTCAATTCCGTTATGCATTATGTTGGGTTCGTTGACACGGACGACATGAACGTTGTTTCTGGGAAAAGAAAATATACAAGCTTAGTGGGTTATTCGGGCAGCAAGCTCGCACAGATTATGTTTAGTAGTGTCTTACATAAGCGGCTGCCTGCTGAAGCTGGTATTTGTGTAACATGTGTTTCTCCCGGTGTTGTCCAGACAAATGTAGCGAGGGATCTTCCCAGAATCATTCAAGCTGGTTATCGTTTGATACCCTATTTTATCTTTAGCCCTCAAGAAGGTTCGAGGAGTGCTCTCTTTGCAGCCACTGATCCTCAGGTTCCAGAGTACTGTCAATTGTTGAAATCAGATGACTTTCCTGTTTGCGCTTTCATTTCTCAAGATTGCAATCCAACCAATCCTTCTGAAGAAGCTCATAATGTTGAGACTTCACACAAAGTGTGGGAAAAGACATTTGAGATGATTGGCCTTCCTTCTGATGCTGTGGAGAAGCTTATAGAAGGGGAAGAAGTTGCATGCAGATATGGGGCTCATAATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001523 GO:0003674 GO:0003824 GO:0004033 GO:0004745 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006066 GO:0006081 GO:0006629 GO:0006720 GO:0006721 GO:0008106 GO:0008150 GO:0008152 GO:0009888 GO:0009987 GO:0010073 GO:0010817 GO:0012505 GO:0016020 GO:0016101 GO:0016491 GO:0016614 GO:0016616 GO:0031984 GO:0032501 GO:0032502 GO:0034308 GO:0034754 GO:0042175 GO:0042445 GO:0042572 GO:0042574 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0052650 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901615 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

adh_short

Protein Analysis

341

Amino Acids

37.55

Weight (kDa)

5.61

Isoelectric Point (pI)

39.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KR	PF08659	24 - 133	1.2e-09	KR domain
adh_short	PF00106	25 - 169	3.6e-26	short chain dehydrogenase
adh_short_C2	PF13561	31 - 235	8.6e-18	Enoyl-(Acyl carrier protein) reductase
adh_short	PF00106	179 - 234	3.9e-06	short chain dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27760 AT5G53090 AT5G53100
fragaria_vesca	FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31671
malus_domestica	MD02G1050600.v1.1 MD02G1208700.v1.1 MD06G1238500.v1.1 MD14G1244800.v1.1 MD16G1170300.v1.1
prunus_persica	Prupe.5G242400_v2.0.a1
pyrus_communis	pycom06g21400 pycom14g20600
rosa_chinensis	RchiOBHm_Chr6g0266921 RchiOBHm_Chr7g0227471
rosa_laevigata	RLG00000001643 RLG00000014057
rosa_multiflora	Rmu_sc0001772.1_g000037 Rmu_sc0001772.1_g000038 Rmu_ssc0000486.1_g000008
rosa_roxburghii	Rroxscaffold_3G00232120 Rroxscaffold_7G00201290
rosa_rugosa	Rorug06G0028300 Rorug06G0028400
rosa_samantha	Rh6AG149400 Rh6BG150300 Rh6CG145200 Rh6DG134500 Rh7AG394300 Rh7CG413400 Rh7DG390500
rosa_wichuraiana	Rw6G012920 Rw7G032830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	108
AciI	CCGC	4 cut(s)	96, 106, 128, 625
AclI	AACGTT	1 cut(s)	534
AclWI	GGATC	5 cut(s)	81, 94, 181, 705, 788
AcoI	YGGCCR	1 cut(s)	103
AcsI	RAATTY	1 cut(s)	368
AcuI	CTGAAG	3 cut(s)	309, 657, 909
AdeI	CACNNNGTG	1 cut(s)	922
AfaI	GTAC	1 cut(s)	812
AfiI	CCNNNNNNNGG	1 cut(s)	761
AflIII	ACRYGT	2 cut(s)	142, 656
AgsI	TTSAA	3 cut(s)	375, 716, 826
AjnI	CCWGG	1 cut(s)	116
AjuI	GAANNNNNNNTTGG	4 cut(s)	290, 322, 868, 900
AluBI	AGCT	8 cut(s)	176, 184, 564, 589, 641, 719, 896, 976
AluI	AGCT	8 cut(s)	176, 184, 564, 589, 641, 719, 896, 976
Alw21I	GWGCWC	3 cut(s)	142, 396, 778
Alw26I	GTCTC	1 cut(s)	902
Alw44I	GTGCAC	1 cut(s)	138
AlwI	GGATC	5 cut(s)	81, 94, 181, 705, 788
AlwNI	CAGNNNCTG	1 cut(s)	791
AoxI	GGCC	3 cut(s)	103, 220, 949
ApaLI	GTGCAC	1 cut(s)	138
ApeKI	GCWGC	4 cut(s)	173, 582, 628, 785
ApoI	RAATTY	1 cut(s)	368
AspLEI	GCGC	1 cut(s)	851
AspS9I	GGNCC	2 cut(s)	220, 311
AsuC2I	CCSGG	1 cut(s)	669
AsuHPI	GGTGA	1 cut(s)	253
AvaII	GGWCC	1 cut(s)	311
AxyI	CCTNAGG	1 cut(s)	798
BaeGI	GKGCMC	1 cut(s)	142
BamHI	GGATCC	1 cut(s)	86
BanII	GRGCYC	1 cut(s)	1015
Bbv12I	GWGCWC	3 cut(s)	142, 396, 778
BbvI	GCAGC	4 cut(s)	185, 594, 615, 797
BccI	CCATC	3 cut(s)	238, 374, 454
BcgI	CGANNNNNNTGC	2 cut(s)	571, 605
BciT130I	CCWGG	1 cut(s)	118
BcnI	CCSGG	1 cut(s)	669
BcoDI	GTCTC	1 cut(s)	902
BfaI	CTAG	1 cut(s)	93
BisI	GCNGC	6 cut(s)	106, 174, 583, 626, 629, 786
BlsI	GCNGC	6 cut(s)	107, 175, 584, 627, 630, 787
BmcAI	AGTACT	1 cut(s)	812
Bme1390I	CCNGG	2 cut(s)	118, 669
Bme18I	GGWCC	1 cut(s)	311
BmgT120I	GGNCC	2 cut(s)	220, 311
BmiI	GGNNCC	4 cut(s)	88, 312, 417, 804
BmrFI	CCNGG	2 cut(s)	118, 669
BmsI	GCATC	1 cut(s)	952
BplI	GAGNNNNNCTC	2 cut(s)	760, 792
BpmI	CTGGAG	1 cut(s)	402
Bpu10I	CCTNAGC	1 cut(s)	177
BpuEI	CTTGAG	2 cut(s)	741, 846
BpuMI	CCSGG	1 cut(s)	669
BsaBI	GATNNNNATC	1 cut(s)	870
BsaJI	CCNNGG	1 cut(s)	264
Bsc4I	CCNNNNNNNGG	1 cut(s)	761
Bse1I	ACTGG	1 cut(s)	88
Bse21I	CCTNAGG	1 cut(s)	798
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	1 cut(s)	118
BseDI	CCNNGG	1 cut(s)	264
BseGI	GGATG	1 cut(s)	446
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	1 cut(s)	761
BseMII	CTCAG	1 cut(s)	812
BseNI	ACTGG	1 cut(s)	88
BseRI	GAGGAG	1 cut(s)	784
BseSI	GKGCMC	1 cut(s)	142
BseX3I	CGGCCG	1 cut(s)	103
BseXI	GCAGC	4 cut(s)	185, 594, 615, 797
BseYI	CCCAGC	1 cut(s)	338
Bsh1236I	CGCG	1 cut(s)	108
Bsh1285I	CGRYCG	1 cut(s)	106
BshFI	GGCC	3 cut(s)	105, 222, 951
BsiEI	CGRYCG	1 cut(s)	106
BsiHKAI	GWGCWC	3 cut(s)	142, 396, 778
BsiSI	CCGG	1 cut(s)	669
BslFI	GGGAC	1 cut(s)	324
BslI	CCNNNNNNNGG	1 cut(s)	761
BsmAI	GTCTC	1 cut(s)	902
BsmFI	GGGAC	1 cut(s)	324
BsmI	GAATGC	1 cut(s)	304
BsnI	GGCC	3 cut(s)	105, 222, 951
Bsp1286I	GDGCHC	4 cut(s)	142, 396, 778, 1015
Bsp143I	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
BspACI	CCGC	4 cut(s)	96, 106, 128, 625
BspANI	GGCC	3 cut(s)	105, 222, 951
BspCNI	CTCAG	1 cut(s)	811
BspFNI	CGCG	1 cut(s)	108
BspLI	GGNNCC	4 cut(s)	88, 312, 417, 804
BspPI	GGATC	5 cut(s)	81, 94, 181, 705, 788
BspQI	GCTCTTC	1 cut(s)	384
BsrI	ACTGG	1 cut(s)	88
BssECI	CCNNGG	1 cut(s)	264
BssMI	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
BssT1I	CCWWGG	1 cut(s)	264
Bst2UI	CCWGG	1 cut(s)	118
Bst4CI	ACNGT	1 cut(s)	815
Bst6I	CTCTTC	2 cut(s)	285, 384
BstC8I	GCNNGC	5 cut(s)	25, 587, 591, 633, 1000
BstDEI	CTNAG	3 cut(s)	177, 565, 798
BstENI	CCTNNNNNAGG	1 cut(s)	759
BstF5I	GGATG	1 cut(s)	446
BstFNI	CGCG	1 cut(s)	108
BstHHI	GCGC	1 cut(s)	851
BstKTI	GATC	6 cut(s)	67, 89, 189, 256, 700, 796
BstMAI	GTCTC	1 cut(s)	902
BstMBI	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
BstMCI	CGRYCG	1 cut(s)	106
BstMWI	GCNNNNNNNGC	2 cut(s)	102, 782
BstNI	CCWGG	1 cut(s)	118
BstNSI	RCATGY	5 cut(s)	75, 146, 323, 660, 1002
BstSCI	CCNGG	2 cut(s)	116, 667
BstSLI	GKGCMC	1 cut(s)	142
BstUI	CGCG	1 cut(s)	108
BstV1I	GCAGC	4 cut(s)	185, 594, 615, 797
BstX2I	RGATCY	2 cut(s)	86, 697
BstXI	CCANNNNNNTGG	1 cut(s)	124
BstYI	RGATCY	2 cut(s)	86, 697
BstZI	CGGCCG	1 cut(s)	103
Bsu36I	CCTNAGG	1 cut(s)	798
BsuRI	GGCC	3 cut(s)	105, 222, 951
BtsCI	GGATG	1 cut(s)	446
BtsI	GCAGTG	3 cut(s)	18, 162, 422
BtsIMutI	CAGTG	5 cut(s)	18, 81, 162, 422, 789
Cac8I	GCNNGC	5 cut(s)	25, 587, 591, 633, 1000
CaiI	CAGNNNCTG	1 cut(s)	791
CfoI	GCGC	1 cut(s)	851
Cfr13I	GGNCC	2 cut(s)	220, 311
Csp6I	GTAC	1 cut(s)	811
CspCI	CAANNNNNGTGG	2 cut(s)	549, 584
CviAII	CATG	6 cut(s)	72, 143, 320, 529, 657, 999
CviQI	GTAC	1 cut(s)	811
DdeI	CTNAG	3 cut(s)	177, 565, 798
DpnI	GATC	6 cut(s)	66, 88, 188, 255, 699, 795
DpnII	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
DraIII	CACNNNGTG	1 cut(s)	922
EaeI	YGGCCR	1 cut(s)	103
EagI	CGGCCG	1 cut(s)	103
Eam1104I	CTCTTC	2 cut(s)	285, 384
EarI	CTCTTC	2 cut(s)	285, 384
EciI	GGCGGA	1 cut(s)	143
EclXI	CGGCCG	1 cut(s)	103
Eco130I	CCWWGG	1 cut(s)	264
Eco24I	GRGCYC	1 cut(s)	1015
Eco47I	GGWCC	1 cut(s)	311
Eco52I	CGGCCG	1 cut(s)	103
Eco57I	CTGAAG	3 cut(s)	309, 657, 909
Eco81I	CCTNAGG	1 cut(s)	798
EcoNI	CCTNNNNNAGG	1 cut(s)	759
EcoO109I	RGGNCCY	1 cut(s)	311
EcoRII	CCWGG	1 cut(s)	116
EcoT14I	CCWWGG	1 cut(s)	264
EcoT22I	ATGCAT	2 cut(s)	77, 501
EcoT38I	GRGCYC	1 cut(s)	1015
ErhI	CCWWGG	1 cut(s)	264
FaeI	CATG	6 cut(s)	75, 146, 323, 532, 660, 1002
FaqI	GGGAC	1 cut(s)	324
FatI	CATG	6 cut(s)	71, 142, 319, 528, 656, 998
Fnu4HI	GCNGC	6 cut(s)	106, 174, 583, 626, 629, 786
FokI	GGATG	1 cut(s)	433
FriOI	GRGCYC	1 cut(s)	1015
Fsp4HI	GCNGC	6 cut(s)	106, 174, 583, 626, 629, 786
FspBI	CTAG	1 cut(s)	93
GlaI	GCGC	1 cut(s)	850
GluI	GCNGC	6 cut(s)	106, 174, 583, 626, 629, 786
GsaI	CCCAGC	1 cut(s)	342
GsuI	CTGGAG	1 cut(s)	402
HaeIII	GGCC	3 cut(s)	105, 222, 951
HapII	CCGG	1 cut(s)	669
HhaI	GCGC	1 cut(s)	851
Hin1II	CATG	6 cut(s)	75, 146, 323, 532, 660, 1002
Hin6I	GCGC	1 cut(s)	849
HinP1I	GCGC	1 cut(s)	849
HincII	GTYRAC	2 cut(s)	61, 517
HindII	GTYRAC	2 cut(s)	61, 517
HindIII	AAGCTT	2 cut(s)	562, 974
HinfI	GANTC	5 cut(s)	15, 131, 269, 406, 708
HpaII	CCGG	1 cut(s)	669
HphI	GGTGA	1 cut(s)	253
Hpy166II	GTNNAC	3 cut(s)	61, 140, 517
Hpy188I	TCNGA	4 cut(s)	289, 832, 889, 961
Hpy188III	TCNNGA	7 cut(s)	10, 190, 213, 679, 758, 806, 863
Hpy8I	GTNNAC	3 cut(s)	61, 140, 517
HpyAV	CCTTC	6 cut(s)	233, 755, 894, 962, 966, 977
HpyCH4III	ACNGT	1 cut(s)	815
HpyCH4IV	ACGT	1 cut(s)	534
HpyF10VI	GCNNNNNNNGC	2 cut(s)	102, 782
HpyF3I	CTNAG	3 cut(s)	177, 565, 798
HpySE526I	ACGT	1 cut(s)	534
Hsp92II	CATG	6 cut(s)	75, 146, 323, 532, 660, 1002
HspAI	GCGC	1 cut(s)	849
Kzo9I	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
LguI	GCTCTTC	1 cut(s)	384
LmnI	GCTCC	2 cut(s)	181, 421
Lsp1109I	GCAGC	4 cut(s)	185, 594, 615, 797
LweI	GCATC	1 cut(s)	952
MaeI	CTAG	1 cut(s)	93
MaeII	ACGT	1 cut(s)	534
MaeIII	GTNAC	2 cut(s)	79, 652
MalI	GATC	6 cut(s)	66, 88, 188, 255, 699, 795
MboI	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
MboII	GAAGA	5 cut(s)	302, 401, 692, 902, 1001
MfeI	CAATTG	1 cut(s)	818
MflI	RGATCY	2 cut(s)	86, 697
MhlI	GDGCHC	4 cut(s)	142, 396, 778, 1015
MluCI	AATT	4 cut(s)	368, 474, 487, 818
MlyI	GAGTC	1 cut(s)	140
MmeI	TCCRAC	1 cut(s)	899
MnlI	CCTC	9 cut(s)	63, 232, 237, 324, 452, 687, 762, 765, 807
Mph1103I	ATGCAT	2 cut(s)	77, 501
MseI	TTAA	1 cut(s)	480
MspI	CCGG	1 cut(s)	669
MspR9I	CCNGG	2 cut(s)	118, 669
MunI	CAATTG	1 cut(s)	818
Mva1269I	GAATGC	1 cut(s)	304
MvaI	CCWGG	1 cut(s)	118
MvnI	CGCG	1 cut(s)	108
MwoI	GCNNNNNNNGC	2 cut(s)	102, 782
NciI	CCSGG	1 cut(s)	669
NdeII	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
NlaIII	CATG	6 cut(s)	75, 146, 323, 532, 660, 1002
NlaIV	GGNNCC	4 cut(s)	88, 312, 417, 804
NmuCI	GTSAC	1 cut(s)	79
NsiI	ATGCAT	2 cut(s)	77, 501
NspI	RCATGY	5 cut(s)	75, 146, 323, 660, 1002
PaeI	GCATGC	1 cut(s)	1002
PciI	ACATGT	2 cut(s)	142, 656
PciSI	GCTCTTC	1 cut(s)	384
PcsI	WCGNNNNNNNCGW	1 cut(s)	531
PctI	GAATGC	1 cut(s)	304
PfeI	GAWTC	4 cut(s)	15, 269, 406, 708
PkrI	GCNGC	6 cut(s)	107, 175, 584, 627, 630, 787
PleI	GAGTC	1 cut(s)	139
PpsI	GAGTC	1 cut(s)	139
PpuMI	RGGWCCY	1 cut(s)	311
PscI	ACATGT	2 cut(s)	142, 656
Psp1406I	AACGTT	1 cut(s)	534
Psp5II	RGGWCCY	1 cut(s)	311
Psp6I	CCWGG	1 cut(s)	116
PspFI	CCCAGC	1 cut(s)	338
PspGI	CCWGG	1 cut(s)	116
PspN4I	GGNNCC	4 cut(s)	88, 312, 417, 804
PspPI	GGNCC	2 cut(s)	220, 311
PspPPI	RGGWCCY	1 cut(s)	311
PsrI	GAACNNNNNNTAC	2 cut(s)	240, 272
PstNI	CAGNNNCTG	1 cut(s)	791
PsuI	RGATCY	2 cut(s)	86, 697
RsaI	GTAC	1 cut(s)	812
RsaNI	GTAC	1 cut(s)	811
SapI	GCTCTTC	1 cut(s)	384
SaqAI	TTAA	1 cut(s)	480
SatI	GCNGC	6 cut(s)	106, 174, 583, 626, 629, 786
Sau3AI	GATC	6 cut(s)	64, 86, 186, 253, 697, 793
Sau96I	GGNCC	2 cut(s)	220, 311
ScaI	AGTACT	1 cut(s)	812
SchI	GAGTC	1 cut(s)	140
ScrFI	CCNGG	2 cut(s)	118, 669
SduI	GDGCHC	4 cut(s)	142, 396, 778, 1015
SfaNI	GCATC	1 cut(s)	952
SinI	GGWCC	1 cut(s)	311
SmlI	CTYRAG	2 cut(s)	756, 861
SmoI	CTYRAG	2 cut(s)	756, 861
SphI	GCATGC	1 cut(s)	1002
Sse9I	AATT	4 cut(s)	368, 474, 487, 818
SsiI	CCGC	4 cut(s)	96, 106, 128, 625
SspMI	CTAG	1 cut(s)	93
StyD4I	CCNGG	2 cut(s)	116, 667
StyI	CCWWGG	1 cut(s)	264
TaaI	ACNGT	1 cut(s)	815
TaiI	ACGT	1 cut(s)	537
TaqI	TCGA	2 cut(s)	472, 767
TasI	AATT	4 cut(s)	368, 474, 487, 818
TatI	WGTACW	1 cut(s)	810
TauI	GCSGC	2 cut(s)	108, 628
TfiI	GAWTC	4 cut(s)	15, 269, 406, 708
Tru1I	TTAA	1 cut(s)	480
Tru9I	TTAA	1 cut(s)	480
TscAI	CASTG	5 cut(s)	25, 88, 162, 429, 796
TseFI	GTSAC	1 cut(s)	79
TseI	GCWGC	4 cut(s)	173, 582, 628, 785
Tsp45I	GTSAC	1 cut(s)	79
TspDTI	ATGAA	5 cut(s)	50, 219, 402, 545, 844
TspGWI	ACGGA	2 cut(s)	481, 536
TspRI	CASTG	5 cut(s)	25, 88, 162, 429, 796
VneI	GTGCAC	1 cut(s)	138
VpaK11BI	GGWCC	1 cut(s)	311
XagI	CCTNNNNNAGG	1 cut(s)	759
XapI	RAATTY	1 cut(s)	368
XceI	RCATGY	5 cut(s)	75, 146, 323, 660, 1002
XspI	CTAG	1 cut(s)	93
ZrmI	AGTACT	1 cut(s)	812
Zsp2I	ATGCAT	2 cut(s)	77, 501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.