Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002204

Physical Location & Seq

Genomic Coordinates Reverse (-)

36926793 .. 36931196

Gene Structure 4404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1238500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGCGGCGTCGGCGGCGTCAAATTCGAAGGAGGAAGTGAAGATGAAGAAGAAGGAAGGATTGGGATGGATAGAGTGGATGAGAGGGTGGTTGTATCTGGCGTACGAGATGCTGTTCCAGAGAATCACGGCCAGCCACTTGCAAAACCCCATGCCTTTGCCTCCCATCAACGATCTCACTTGCATTGTCACTGGATCCACCAGCGGCATCGGCCGCGAAATCGCCAGGCAACTAGCGGAATCAGGCGCGCATGTTGTAATGGCGGTGAGGAATACGAAAGCAGCTAATGAGCTGATCCAGAAGTGGCAAAATGAATGGTCTGGAATGGGGCTTCCTCTCAATATTGAGGTTATGGAACTTGATCTGCTTTCATTGGATTCTGTTGTGAGATTTGCCGAAGCATGGAACGCTCGTTTAGGACCTCTCCATGTTCTTATCAACAATGCTGGGATATTTTCAATTGGAGAGCCGCAAAAGTTTTCTAAAGATGGTTACGAAGAACACTTGCAAGTGAATCATCTGGCTCCAGCGTTGCTATCCGTCTTGCTATTGCCTTCCCTTATTAGAGGATCTCCGAGTCGAATTGTTAATTTGAATTCCGTTATGCATTATGTTGGCTTTGTTGACACAGAAGACATGAACGTTGTTTCCGGGAAAAGAAAATATACAAGCTTAGTGGGTTACTCAAGCAGCAAGTTGGCACAGATTATGTTTAGTAGTGTCCTACATAAGCGGCTGCCTGCTGAATCTGGCATTAGTGTAGTGTGCGTTTCACCTGGAATTGTCCAGACTAATGTTGCAAGGGATCTACCCAGGATTGTTCAGGCTGCGTATCATCTAGTACCTTATTTTATGTTTAGTGCTCAAGAAGGTTCTAGGAGTGCTCTGTTTGCAGCCACTGATCCACAGGTTCCGGAGTACTGTGAATTGTTGAAAACGGATGACTTTCCTGTTTGTGCCTTTATTTCTCAAGATTGCCGTCCAACGAATCCTTCAGAAGAATCGCATAATGTTGACACTTGTCACGAAGTGTGGGAGAAGACATTTGAGATGATTGGCCTTCCTTCTGATGCAGTGGAGAGGCTTATAGAAGGGGAAGAAGTTGCATGCAGATACGGGGCTCATGGGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001523 GO:0003674 GO:0003824 GO:0004033 GO:0004745 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006066 GO:0006081 GO:0006629 GO:0006720 GO:0006721 GO:0008106 GO:0008150 GO:0008152 GO:0009888 GO:0009987 GO:0010073 GO:0010817 GO:0012505 GO:0016020 GO:0016101 GO:0016491 GO:0016614 GO:0016616 GO:0031984 GO:0032501 GO:0032502 GO:0034308 GO:0034754 GO:0042175 GO:0042445 GO:0042572 GO:0042574 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048507 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051239 GO:0052650 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0098827 GO:1901615 GO:2000024 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

adh_short

Protein Analysis

378

Amino Acids

41.73

Weight (kDa)

5.53

Isoelectric Point (pI)

44.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KR	PF08659	60 - 170	1.6e-09	KR domain
adh_short	PF00106	61 - 205	2.7e-25	short chain dehydrogenase
adh_short_C2	PF13561	67 - 272	5.9e-18	Enoyl-(Acyl carrier protein) reductase
adh_short	PF00106	215 - 270	2.9e-07	short chain dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27760 AT5G53090 AT5G53100
fragaria_vesca	FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31670 FvH4_5g31671
malus_domestica	MD02G1050600.v1.1 MD02G1208700.v1.1 MD06G1238500.v1.1 MD14G1244800.v1.1 MD16G1170300.v1.1
prunus_persica	Prupe.5G242400_v2.0.a1
pyrus_communis	pycom06g21400 pycom14g20600
rosa_chinensis	RchiOBHm_Chr6g0266921 RchiOBHm_Chr7g0227471
rosa_laevigata	RLG00000001643 RLG00000014057
rosa_multiflora	Rmu_sc0001772.1_g000037 Rmu_sc0001772.1_g000038 Rmu_ssc0000486.1_g000008
rosa_roxburghii	Rroxscaffold_3G00232120 Rroxscaffold_7G00201290
rosa_rugosa	Rorug06G0028300 Rorug06G0028400
rosa_samantha	Rh6AG149400 Rh6BG150300 Rh6CG145200 Rh6DG134500 Rh7AG394300 Rh7CG413400 Rh7DG390500
rosa_wichuraiana	Rw6G012920 Rw7G032830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	216, 248
AccIII	TCCGGA	1 cut(s)	913
AciI	CCGC	8 cut(s)	5, 14, 204, 214, 236, 263, 470, 733
AclI	AACGTT	1 cut(s)	642
AclWI	GGATC	6 cut(s)	189, 202, 289, 577, 813, 896
AcoI	YGGCCR	2 cut(s)	129, 211
AcsI	RAATTY	2 cut(s)	22, 595
AcuI	CTGAAG	1 cut(s)	978
AcyI	GRCGYC	2 cut(s)	8, 17
AdeI	CACNNNGTG	1 cut(s)	1030
AfaI	GTAC	3 cut(s)	104, 843, 920
AfiI	CCNNNNNNNGG	1 cut(s)	402
AgsI	TTSAA	3 cut(s)	459, 595, 934
AjnI	CCWGG	3 cut(s)	224, 775, 812
AjuI	GAANNNNNNNTTGG	4 cut(s)	44, 76, 976, 1008
AloI	GAACNNNNNNTCC	2 cut(s)	632, 664
AluBI	AGCT	3 cut(s)	284, 292, 672
AluI	AGCT	3 cut(s)	284, 292, 672
Alw21I	GWGCWC	2 cut(s)	865, 886
AlwI	GGATC	6 cut(s)	189, 202, 289, 577, 813, 896
AlwNI	CAGNNNCTG	1 cut(s)	899
Aor13HI	TCCGGA	1 cut(s)	913
AoxI	GGCC	3 cut(s)	129, 211, 1057
ApeKI	GCWGC	5 cut(s)	281, 690, 736, 827, 893
ApoI	RAATTY	2 cut(s)	22, 595
AspLEI	GCGC	2 cut(s)	248, 250
AspS9I	GGNCC	1 cut(s)	419
AsuC2I	CCSGG	1 cut(s)	652
AsuHPI	GGTGA	2 cut(s)	277, 765
AsuII	TTCGAA	1 cut(s)	26
AvaII	GGWCC	1 cut(s)	419
BamHI	GGATCC	1 cut(s)	194
BanII	GRGCYC	1 cut(s)	1123
BbsI	GAAGAC	2 cut(s)	639, 1046
Bbv12I	GWGCWC	2 cut(s)	865, 886
BbvI	GCAGC	5 cut(s)	293, 702, 723, 814, 905
BccI	CCATC	3 cut(s)	60, 173, 482
BceAI	ACGGC	2 cut(s)	144, 963
BciT130I	CCWGG	3 cut(s)	226, 777, 814
BcnI	CCSGG	1 cut(s)	652
BfaI	CTAG	3 cut(s)	233, 839, 876
BmcAI	AGTACT	1 cut(s)	920
Bme1390I	CCNGG	4 cut(s)	226, 652, 777, 814
Bme18I	GGWCC	1 cut(s)	419
BmgT120I	GGNCC	1 cut(s)	419
BmiI	GGNNCC	3 cut(s)	196, 525, 912
BmrFI	CCNGG	4 cut(s)	226, 652, 777, 814
BmsI	GCATC	3 cut(s)	99, 216, 1060
BoxI	GACNNNNGTC	1 cut(s)	1020
BpiI	GAAGAC	2 cut(s)	639, 1046
BpmI	CTGGAG	1 cut(s)	510
Bpu14I	TTCGAA	1 cut(s)	26
BpuEI	CTTGAG	3 cut(s)	670, 849, 954
BpuMI	CCSGG	1 cut(s)	652
BsaHI	GRCGYC	2 cut(s)	8, 17
BsaJI	CCNNGG	1 cut(s)	812
BsaWI	WCCGGW	1 cut(s)	913
Bsc4I	CCNNNNNNNGG	1 cut(s)	402
Bse1I	ACTGG	1 cut(s)	196
BseAI	TCCGGA	1 cut(s)	913
BseBI	CCWGG	3 cut(s)	226, 777, 814
BseDI	CCNNGG	1 cut(s)	812
BseGI	GGATG	3 cut(s)	71, 84, 946
BseLI	CCNNNNNNNGG	1 cut(s)	402
BseNI	ACTGG	1 cut(s)	196
BsePI	GCGCGC	1 cut(s)	246
BseX3I	CGGCCG	1 cut(s)	211
BseXI	GCAGC	5 cut(s)	293, 702, 723, 814, 905
BseYI	CCCAGC	1 cut(s)	446
Bsh1236I	CGCG	2 cut(s)	216, 248
Bsh1285I	CGRYCG	1 cut(s)	214
BshFI	GGCC	3 cut(s)	131, 213, 1059
BsiEI	CGRYCG	1 cut(s)	214
BsiHKAI	GWGCWC	2 cut(s)	865, 886
BsiSI	CCGG	2 cut(s)	651, 914
BsiWI	CGTACG	1 cut(s)	102
BslI	CCNNNNNNNGG	1 cut(s)	402
BsnI	GGCC	3 cut(s)	131, 213, 1059
Bsp119I	TTCGAA	1 cut(s)	26
Bsp1286I	GDGCHC	3 cut(s)	865, 886, 1123
Bsp13I	TCCGGA	1 cut(s)	913
Bsp143I	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
BspACI	CCGC	8 cut(s)	5, 14, 204, 214, 236, 263, 470, 733
BspANI	GGCC	3 cut(s)	131, 213, 1059
BspEI	TCCGGA	1 cut(s)	913
BspFNI	CGCG	2 cut(s)	216, 248
BspLI	GGNNCC	3 cut(s)	196, 525, 912
BspPI	GGATC	6 cut(s)	189, 202, 289, 577, 813, 896
BspT104I	TTCGAA	1 cut(s)	26
BsrI	ACTGG	1 cut(s)	196
BssECI	CCNNGG	1 cut(s)	812
BssHII	GCGCGC	1 cut(s)	246
BssMI	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
BssNI	GRCGYC	2 cut(s)	8, 17
Bst2UI	CCWGG	3 cut(s)	226, 777, 814
Bst4CI	ACNGT	1 cut(s)	923
BstACI	GRCGYC	2 cut(s)	8, 17
BstBI	TTCGAA	1 cut(s)	26
BstC8I	GCNNGC	4 cut(s)	133, 248, 741, 1108
BstDEI	CTNAG	1 cut(s)	673
BstF5I	GGATG	3 cut(s)	71, 84, 946
BstFNI	CGCG	2 cut(s)	216, 248
BstHHI	GCGC	2 cut(s)	248, 250
BstKTI	GATC	7 cut(s)	175, 197, 297, 364, 572, 808, 904
BstMBI	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
BstMCI	CGRYCG	1 cut(s)	214
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 14, 210, 213, 407, 890
BstNI	CCWGG	3 cut(s)	226, 777, 814
BstNSI	RCATGY	2 cut(s)	254, 1110
BstPAI	GACNNNNGTC	1 cut(s)	1020
BstSCI	CCNGG	4 cut(s)	224, 650, 775, 812
BstUI	CGCG	2 cut(s)	216, 248
BstV1I	GCAGC	5 cut(s)	293, 702, 723, 814, 905
BstV2I	GAAGAC	2 cut(s)	639, 1046
BstX2I	RGATCY	3 cut(s)	194, 569, 805
BstYI	RGATCY	3 cut(s)	194, 569, 805
BstZI	CGGCCG	1 cut(s)	211
BsuRI	GGCC	3 cut(s)	131, 213, 1059
BtsCI	GGATG	3 cut(s)	71, 84, 946
BtsI	GCAGTG	1 cut(s)	1080
BtsIMutI	CAGTG	3 cut(s)	189, 897, 1080
Cac8I	GCNNGC	4 cut(s)	133, 248, 741, 1108
CaiI	CAGNNNCTG	1 cut(s)	899
CfoI	GCGC	2 cut(s)	248, 250
Cfr13I	GGNCC	1 cut(s)	419
CseI	GACGC	1 cut(s)	6
Csp6I	GTAC	3 cut(s)	103, 842, 919
CspCI	CAANNNNNGTGG	2 cut(s)	657, 692
CviAII	CATG	7 cut(s)	151, 251, 402, 428, 637, 1107, 1124
CviQI	GTAC	3 cut(s)	103, 842, 919
DdeI	CTNAG	1 cut(s)	673
DpnI	GATC	7 cut(s)	174, 196, 296, 363, 571, 807, 903
DpnII	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
DraIII	CACNNNGTG	1 cut(s)	1030
EaeI	YGGCCR	2 cut(s)	129, 211
EagI	CGGCCG	1 cut(s)	211
EclXI	CGGCCG	1 cut(s)	211
Eco24I	GRGCYC	1 cut(s)	1123
Eco47I	GGWCC	1 cut(s)	419
Eco52I	CGGCCG	1 cut(s)	211
Eco57I	CTGAAG	1 cut(s)	978
EcoO109I	RGGNCCY	1 cut(s)	419
EcoRI	GAATTC	1 cut(s)	595
EcoRII	CCWGG	3 cut(s)	224, 775, 812
EcoT22I	ATGCAT	1 cut(s)	609
EcoT38I	GRGCYC	1 cut(s)	1123
FaeI	CATG	7 cut(s)	154, 254, 405, 431, 640, 1110, 1127
FatI	CATG	7 cut(s)	150, 250, 401, 427, 636, 1106, 1123
FokI	GGATG	3 cut(s)	78, 91, 953
FriOI	GRGCYC	1 cut(s)	1123
FspBI	CTAG	3 cut(s)	233, 839, 876
GlaI	GCGC	2 cut(s)	247, 249
GsaI	CCCAGC	1 cut(s)	450
GsuI	CTGGAG	1 cut(s)	510
HaeIII	GGCC	3 cut(s)	131, 213, 1059
HapII	CCGG	2 cut(s)	651, 914
HgaI	GACGC	1 cut(s)	6
HhaI	GCGC	2 cut(s)	248, 250
Hin1I	GRCGYC	2 cut(s)	8, 17
Hin1II	CATG	7 cut(s)	154, 254, 405, 431, 640, 1110, 1127
Hin6I	GCGC	2 cut(s)	246, 248
HinP1I	GCGC	2 cut(s)	246, 248
HincII	GTYRAC	2 cut(s)	625, 1015
HindII	GTYRAC	2 cut(s)	625, 1015
HindIII	AAGCTT	1 cut(s)	670
HinfI	GANTC	8 cut(s)	123, 239, 377, 514, 577, 746, 988, 1001
HpaII	CCGG	2 cut(s)	651, 914
HphI	GGTGA	2 cut(s)	277, 765
Hpy166II	GTNNAC	2 cut(s)	625, 1015
Hpy188I	TCNGA	3 cut(s)	576, 997, 1069
Hpy188III	TCNNGA	8 cut(s)	118, 298, 321, 787, 866, 914, 971, 1025
Hpy8I	GTNNAC	2 cut(s)	625, 1015
Hpy99I	CGWCG	1 cut(s)	13
HpyAV	CCTTC	9 cut(s)	22, 46, 50, 564, 863, 1002, 1070, 1074, 1085
HpyCH4III	ACNGT	1 cut(s)	923
HpyCH4IV	ACGT	1 cut(s)	642
HpyCH4V	TGCA	9 cut(s)	142, 183, 508, 607, 800, 893, 1073, 1106, 1110
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 14, 210, 213, 407, 890
HpyF3I	CTNAG	1 cut(s)	673
HpySE526I	ACGT	1 cut(s)	642
Hsp92I	GRCGYC	2 cut(s)	8, 17
Hsp92II	CATG	7 cut(s)	154, 254, 405, 431, 640, 1110, 1127
HspAI	GCGC	2 cut(s)	246, 248
Kpn2I	TCCGGA	1 cut(s)	913
Kzo9I	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
LmnI	GCTCC	1 cut(s)	529
Lsp1109I	GCAGC	5 cut(s)	293, 702, 723, 814, 905
LweI	GCATC	3 cut(s)	99, 216, 1060
MaeI	CTAG	3 cut(s)	233, 839, 876
MaeII	ACGT	1 cut(s)	642
MaeIII	GTNAC	4 cut(s)	187, 491, 680, 1022
MalI	GATC	7 cut(s)	174, 196, 296, 363, 571, 807, 903
MboI	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
MboII	GAAGA	8 cut(s)	52, 58, 61, 509, 644, 1010, 1051, 1109
MfeI	CAATTG	1 cut(s)	459
MflI	RGATCY	3 cut(s)	194, 569, 805
MhlI	GDGCHC	3 cut(s)	865, 886, 1123
MluCI	AATT	7 cut(s)	22, 459, 582, 589, 595, 780, 926
MlyI	GAGTC	1 cut(s)	586
MmeI	TCCRAC	1 cut(s)	1007
MnlI	CCTC	9 cut(s)	25, 77, 171, 261, 340, 345, 432, 560, 1074
Mph1103I	ATGCAT	1 cut(s)	609
MroI	TCCGGA	1 cut(s)	913
MseI	TTAA	1 cut(s)	588
MspA1I	CMGCKG	1 cut(s)	204
MspI	CCGG	2 cut(s)	651, 914
MspR9I	CCNGG	4 cut(s)	226, 652, 777, 814
MunI	CAATTG	1 cut(s)	459
MvaI	CCWGG	3 cut(s)	226, 777, 814
MvnI	CGCG	2 cut(s)	216, 248
MwoI	GCNNNNNNNGC	6 cut(s)	11, 14, 210, 213, 407, 890
NciI	CCSGG	1 cut(s)	652
NdeII	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
NlaIII	CATG	7 cut(s)	154, 254, 405, 431, 640, 1110, 1127
NlaIV	GGNNCC	3 cut(s)	196, 525, 912
NmuCI	GTSAC	2 cut(s)	187, 1022
NsiI	ATGCAT	1 cut(s)	609
NspI	RCATGY	2 cut(s)	254, 1110
NspV	TTCGAA	1 cut(s)	26
PaeI	GCATGC	1 cut(s)	1110
PauI	GCGCGC	1 cut(s)	246
PfeI	GAWTC	7 cut(s)	123, 239, 377, 514, 746, 988, 1001
Pfl23II	CGTACG	1 cut(s)	102
PfoI	TCCNGGA	1 cut(s)	650
PleI	GAGTC	1 cut(s)	585
PpsI	GAGTC	1 cut(s)	585
PpuMI	RGGWCCY	1 cut(s)	419
PshAI	GACNNNNGTC	1 cut(s)	1020
Psp1406I	AACGTT	1 cut(s)	642
Psp5II	RGGWCCY	1 cut(s)	419
Psp6I	CCWGG	3 cut(s)	224, 775, 812
PspFI	CCCAGC	1 cut(s)	446
PspGI	CCWGG	3 cut(s)	224, 775, 812
PspLI	CGTACG	1 cut(s)	102
PspN4I	GGNNCC	3 cut(s)	196, 525, 912
PspPI	GGNCC	1 cut(s)	419
PspPPI	RGGWCCY	1 cut(s)	419
PstNI	CAGNNNCTG	1 cut(s)	899
PsuI	RGATCY	3 cut(s)	194, 569, 805
PteI	GCGCGC	1 cut(s)	246
RsaI	GTAC	3 cut(s)	104, 843, 920
RsaNI	GTAC	3 cut(s)	103, 842, 919
SaqAI	TTAA	1 cut(s)	588
Sau3AI	GATC	7 cut(s)	172, 194, 294, 361, 569, 805, 901
Sau96I	GGNCC	1 cut(s)	419
ScaI	AGTACT	1 cut(s)	920
SchI	GAGTC	1 cut(s)	586
ScrFI	CCNGG	4 cut(s)	226, 652, 777, 814
SduI	GDGCHC	3 cut(s)	865, 886, 1123
SfaNI	GCATC	3 cut(s)	99, 216, 1060
SfuI	TTCGAA	1 cut(s)	26
SinI	GGWCC	1 cut(s)	419
SmlI	CTYRAG	3 cut(s)	685, 864, 969
SmoI	CTYRAG	3 cut(s)	685, 864, 969
SphI	GCATGC	1 cut(s)	1110
Sse9I	AATT	7 cut(s)	22, 459, 582, 589, 595, 780, 926
SsiI	CCGC	8 cut(s)	5, 14, 204, 214, 236, 263, 470, 733
SspI	AATATT	1 cut(s)	343
SspMI	CTAG	3 cut(s)	233, 839, 876
StyD4I	CCNGG	4 cut(s)	224, 650, 775, 812
TaaI	ACNGT	1 cut(s)	923
TaiI	ACGT	1 cut(s)	645
TaqI	TCGA	2 cut(s)	26, 580
TasI	AATT	7 cut(s)	22, 459, 582, 589, 595, 780, 926
TatI	WGTACW	1 cut(s)	918
TauI	GCSGC	6 cut(s)	8, 17, 207, 216, 472, 736
TfiI	GAWTC	7 cut(s)	123, 239, 377, 514, 746, 988, 1001
Tru1I	TTAA	1 cut(s)	588
Tru9I	TTAA	1 cut(s)	588
TscAI	CASTG	3 cut(s)	196, 904, 1080
TseFI	GTSAC	2 cut(s)	187, 1022
TseI	GCWGC	5 cut(s)	281, 690, 736, 827, 893
Tsp45I	GTSAC	2 cut(s)	187, 1022
TspDTI	ATGAA	4 cut(s)	59, 327, 360, 653
TspGWI	ACGGA	3 cut(s)	529, 589, 953
TspRI	CASTG	3 cut(s)	196, 904, 1080
VpaK11BI	GGWCC	1 cut(s)	419
XapI	RAATTY	2 cut(s)	22, 595
XceI	RCATGY	2 cut(s)	254, 1110
XspI	CTAG	3 cut(s)	233, 839, 876
ZrmI	AGTACT	1 cut(s)	920
Zsp2I	ATGCAT	1 cut(s)	609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.