AT4G28360

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002533
Physical Location & Seq
Reverse (-)
14028983 .. 14031049
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28360.1

Sequence Viewer

Length: 816 bp
ATGGTGGGTTGGAAGAGGAATTTGCAGACTGTTATTCGTCAAGTTGGTAGAAGAGTGAAGAACAGTCACATTTCTACAGCAAATTACTCTTCTTCTACTCGGAATTTAGAATCCCCTTTCTCACAAGGTTACTTGCAGAGTCTCCTGAGACCATCCTACTCCTCCAGACCACTGTATCATCATCTACAACAACTGGGGATCTCTACCTCGAGACAATTGCAGGCGAGTGAAGAGCCTGTATCATCACCTTTGTCATCTCCAGCTCTGTTGGGTAGTGGAAAAGAAGAAGAGCAGAAGATTATCCCAAAGCGTCAGAAAGTTCAGGCTGTCCTCAAGTCTATAAAGCAGAGTCCTAAGAAGGTCAACCTGGTTGCAGCACTAGTCCGTGGCATGCGTGTTGAAGATGCTTTGATCCAATTGCAGGTCACAGTCAAACGAGCTGCACAAACTGTGTACCGGGTTATCCACGCTGCCCGGGCAAATGCTACTCATAACCATGGACTAGATCCTGACCGTCTCCTTGTTGCGGAAGCATTTGTTGGGAAGGGACTGTTTGGGAAGAAGGTAGCTTACCATGCAAAAGGAAGAAGCGGGATTATATCAATACCCCGGTGTCGCCTAACAGTCATAGTTAGAGAGACGACTCCAGAGGAAGAAGCTGAGATTGCAAGGCTCAAAGTTCACAATTTTAAGAAGAAAAGCAAACGGGAGAGACAGCTTGTACCACACAAGCTCATCGAGACAAGTCCAATATGGAACCGCAGAGGTACCAAAGCCAATCATCGGTCCTCAGAGTTGGTACGGTCGTCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

271

Amino Acids

30.46

Weight (kDa)

10.92

Isoelectric Point (pI)

66.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 109 - 212 1.1e-28 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
Acc65I GGTACC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 767
AciI CCGC 3 cut(s) 527, 591, 760
AclWI GGATC 3 cut(s) 206, 406, 500
AcsI RAATTY 2 cut(s) 19, 103
AfaI GTAC 4 cut(s) 455, 723, 769, 801
AfiI CCNNNNNNNGG 3 cut(s) 421, 526, 783
AgsI TTSAA 1 cut(s) 401
AhlI ACTAGT 1 cut(s) 379
AjnI CCWGG 1 cut(s) 366
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 6 cut(s) 263, 440, 569, 659, 718, 733
AluI AGCT 6 cut(s) 263, 440, 569, 659, 718, 733
Alw26I GTCTC 7 cut(s) 142, 146, 205, 521, 632, 706, 734
AlwI GGATC 3 cut(s) 206, 406, 500
Ama87I CYCGRG 2 cut(s) 208, 474
ApeKI GCWGC 3 cut(s) 374, 440, 470
ApoI RAATTY 2 cut(s) 19, 103
Asp718I GGTACC 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 786
AsuC2I CCSGG 4 cut(s) 458, 475, 476, 610
AsuHPI GGTGA 1 cut(s) 237
AvaI CYCGRG 2 cut(s) 208, 474
AvaII GGWCC 1 cut(s) 786
BaeI ACNNNNGTAYC 3 cut(s) 158, 191, 791
BanI GGYRCC 1 cut(s) 767
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BbvI GCAGC 3 cut(s) 386, 427, 457
BccI CCATC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BciT130I CCWGG 1 cut(s) 368
BcnI CCSGG 4 cut(s) 458, 475, 476, 610
BcoDI GTCTC 7 cut(s) 142, 146, 205, 521, 632, 706, 734
BcuI ACTAGT 1 cut(s) 379
BfaI CTAG 3 cut(s) 380, 503, 814
BfmI CTRYAG 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 3 cut(s) 375, 441, 471
BlsI GCNGC 3 cut(s) 376, 442, 472
Bme1390I CCNGG 5 cut(s) 368, 458, 475, 476, 610
Bme18I GGWCC 1 cut(s) 786
BmeT110I CYCGRG 2 cut(s) 208, 474
BmgT120I GGNCC 1 cut(s) 786
BmiI GGNNCC 2 cut(s) 758, 769
BmrFI CCNGG 5 cut(s) 368, 458, 475, 476, 610
BmrI ACTGGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 394
BmuI ACTGGG 1 cut(s) 203
BpmI CTGGAG 3 cut(s) 148, 243, 630
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 4 cut(s) 458, 475, 476, 610
BsaI GGTCTC 1 cut(s) 142
BsaJI CCNNGG 4 cut(s) 385, 474, 496, 608
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 526, 783
Bse1I ACTGG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 4 cut(s) 385, 474, 496, 608
BseGI GGATG 1 cut(s) 152
BseLI CCNNNNNNNGG 3 cut(s) 421, 526, 783
BseMII CTCAG 3 cut(s) 137, 651, 804
BseNI ACTGG 1 cut(s) 198
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 386, 427, 457
BsgI GTGCAG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 806
BshNI GGYRCC 1 cut(s) 767
BsiEI CGRYCG 1 cut(s) 806
BsiHKCI CYCGRG 2 cut(s) 208, 474
BsiSI CCGG 3 cut(s) 457, 475, 610
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 3 cut(s) 421, 526, 783
BsmAI GTCTC 7 cut(s) 142, 146, 205, 521, 632, 706, 734
BsmBI CGTCTC 2 cut(s) 521, 632
BsmFI GGGAC 1 cut(s) 561
Bso31I GGTCTC 1 cut(s) 142
BsoBI CYCGRG 2 cut(s) 208, 474
Bsp143I GATC 3 cut(s) 198, 411, 505
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 3 cut(s) 527, 591, 760
BspCNI CTCAG 3 cut(s) 138, 652, 803
BspLI GGNNCC 2 cut(s) 758, 769
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 3 cut(s) 206, 406, 500
BspQI GCTCTTC 2 cut(s) 225, 282
BspT107I GGYRCC 1 cut(s) 767
BspTNI GGTCTC 1 cut(s) 142
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 4 cut(s) 385, 474, 496, 608
BssMI GATC 3 cut(s) 198, 411, 505
BssT1I CCWWGG 1 cut(s) 496
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 9 cut(s) 31, 65, 174, 430, 451, 515, 552, 625, 804
Bst6I CTCTTC 5 cut(s) 8, 46, 94, 225, 282
BstC8I GCNNGC 2 cut(s) 222, 392
BstDEI CTNAG 4 cut(s) 146, 354, 660, 790
BstDSI CCRYGG 2 cut(s) 385, 496
BstF5I GGATG 1 cut(s) 152
BstKTI GATC 3 cut(s) 201, 414, 508
BstMAI GTCTC 7 cut(s) 142, 146, 205, 521, 632, 706, 734
BstMBI GATC 3 cut(s) 198, 411, 505
BstMCI CGRYCG 1 cut(s) 806
BstMWI GCNNNNNNNGC 3 cut(s) 476, 575, 665
BstNI CCWGG 1 cut(s) 368
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 5 cut(s) 366, 456, 473, 474, 608
BstSFI CTRYAG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 386, 427, 457
BstX2I RGATCY 2 cut(s) 198, 505
BstYI RGATCY 2 cut(s) 198, 505
BtgI CCRYGG 2 cut(s) 385, 496
BtsCI GGATG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 170
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 222, 392
Cfr13I GGNCC 1 cut(s) 786
Cfr9I CCCGGG 1 cut(s) 474
CseI GACGC 1 cut(s) 299
CsiI ACCWGGT 1 cut(s) 366
Csp6I GTAC 4 cut(s) 454, 722, 768, 800
CviAII CATG 3 cut(s) 391, 497, 575
CviQI GTAC 4 cut(s) 454, 722, 768, 800
DdeI CTNAG 4 cut(s) 146, 354, 660, 790
DpnI GATC 3 cut(s) 200, 413, 507
DpnII GATC 3 cut(s) 198, 411, 505
Eam1104I CTCTTC 5 cut(s) 8, 46, 94, 225, 282
EarI CTCTTC 5 cut(s) 8, 46, 94, 225, 282
Eco130I CCWWGG 1 cut(s) 496
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 786
Eco88I CYCGRG 2 cut(s) 208, 474
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 496
Esp3I CGTCTC 2 cut(s) 521, 632
FaeI CATG 3 cut(s) 394, 500, 578
FaiI YATR 8 cut(s) 341, 392, 492, 498, 576, 599, 629, 754
FaqI GGGAC 1 cut(s) 561
FatI CATG 3 cut(s) 390, 496, 574
FauI CCCGC 1 cut(s) 584
Fnu4HI GCNGC 3 cut(s) 375, 441, 471
FokI GGATG 1 cut(s) 139
Fsp4HI GCNGC 3 cut(s) 375, 441, 471
FspBI CTAG 3 cut(s) 380, 503, 814
GluI GCNGC 3 cut(s) 375, 441, 471
GsuI CTGGAG 3 cut(s) 148, 243, 630
HapII CCGG 3 cut(s) 457, 475, 610
HgaI GACGC 1 cut(s) 299
Hin1II CATG 3 cut(s) 394, 500, 578
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 4 cut(s) 110, 139, 349, 643
HpaII CCGG 3 cut(s) 457, 475, 610
HphI GGTGA 1 cut(s) 237
Hpy166II GTNNAC 3 cut(s) 364, 454, 682
Hpy188I TCNGA 3 cut(s) 102, 315, 793
Hpy188III TCNNGA 6 cut(s) 145, 165, 210, 509, 647, 739
Hpy8I GTNNAC 3 cut(s) 364, 454, 682
HpyAV CCTTC 3 cut(s) 352, 538, 556
HpyCH4III ACNGT 9 cut(s) 31, 65, 174, 430, 451, 515, 552, 625, 804
HpyCH4V TGCA 8 cut(s) 25, 136, 220, 374, 421, 443, 578, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 575, 665
HpyF3I CTNAG 4 cut(s) 146, 354, 660, 790
Hsp92II CATG 3 cut(s) 394, 500, 578
KpnI GGTACC 1 cut(s) 771
Kzo9I GATC 3 cut(s) 198, 411, 505
LguI GCTCTTC 2 cut(s) 225, 282
Lsp1109I GCAGC 3 cut(s) 386, 427, 457
LweI GCATC 1 cut(s) 394
MabI ACCWGGT 1 cut(s) 366
MaeI CTAG 3 cut(s) 380, 503, 814
MaeIII GTNAC 3 cut(s) 65, 128, 424
MalI GATC 3 cut(s) 200, 413, 507
MboI GATC 3 cut(s) 198, 411, 505
MfeI CAATTG 2 cut(s) 215, 416
MflI RGATCY 2 cut(s) 198, 505
MluCI AATT 6 cut(s) 19, 82, 103, 215, 416, 685
MlyI GAGTC 3 cut(s) 148, 358, 637
MnlI CCTC 7 cut(s) 9, 172, 217, 341, 643, 758, 799
MseI TTAA 1 cut(s) 690
MslI CAYNNNNRTG 1 cut(s) 495
MspI CCGG 3 cut(s) 457, 475, 610
MspR9I CCNGG 5 cut(s) 368, 458, 475, 476, 610
MunI CAATTG 2 cut(s) 215, 416
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 3 cut(s) 476, 575, 665
NciI CCSGG 4 cut(s) 458, 475, 476, 610
NcoI CCATGG 1 cut(s) 496
NdeII GATC 3 cut(s) 198, 411, 505
NlaIII CATG 3 cut(s) 394, 500, 578
NlaIV GGNNCC 2 cut(s) 758, 769
NmuCI GTSAC 2 cut(s) 65, 424
NspI RCATGY 1 cut(s) 394
PaeI GCATGC 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 208
PciSI GCTCTTC 2 cut(s) 225, 282
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 376, 442, 472
PleI GAGTC 3 cut(s) 147, 357, 637
PpsI GAGTC 3 cut(s) 147, 357, 637
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 2 cut(s) 758, 769
PspPI GGNCC 1 cut(s) 786
PsuI RGATCY 2 cut(s) 198, 505
RsaI GTAC 4 cut(s) 455, 723, 769, 801
RsaNI GTAC 4 cut(s) 454, 722, 768, 800
RseI CAYNNNNRTG 1 cut(s) 495
SapI GCTCTTC 2 cut(s) 225, 282
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 3 cut(s) 375, 441, 471
Sau3AI GATC 3 cut(s) 198, 411, 505
Sau96I GGNCC 1 cut(s) 786
SchI GAGTC 3 cut(s) 148, 358, 637
ScrFI CCNGG 5 cut(s) 368, 458, 475, 476, 610
SexAI ACCWGGT 1 cut(s) 366
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 208
SinI GGWCC 1 cut(s) 786
SlaI CTCGAG 1 cut(s) 208
SmaI CCCGGG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 2 cut(s) 208, 332
SmoI CTYRAG 2 cut(s) 208, 332
SpeI ACTAGT 1 cut(s) 379
SphI GCATGC 1 cut(s) 394
SrfI GCCCGGGC 1 cut(s) 476
Sse9I AATT 6 cut(s) 19, 82, 103, 215, 416, 685
SsiI CCGC 3 cut(s) 527, 591, 760
SspMI CTAG 3 cut(s) 380, 503, 814
StyD4I CCNGG 5 cut(s) 366, 456, 473, 474, 608
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 9 cut(s) 31, 65, 174, 430, 451, 515, 552, 625, 804
TaqI TCGA 2 cut(s) 209, 738
TaqII GACCGA 1 cut(s) 774
TasI AATT 6 cut(s) 19, 82, 103, 215, 416, 685
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 2 cut(s) 65, 424
TseI GCWGC 3 cut(s) 374, 440, 470
Tsp45I GTSAC 2 cut(s) 65, 424
TspGWI ACGGA 1 cut(s) 374
TspMI CCCGGG 1 cut(s) 474
TspRI CASTG 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 786
XapI RAATTY 2 cut(s) 19, 103
XceI RCATGY 1 cut(s) 394
XhoI CTCGAG 1 cut(s) 208
XmaI CCCGGG 1 cut(s) 474
XspI CTAG 3 cut(s) 380, 503, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.