MD14G1042400.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002533
Physical Location & Seq
Reverse (-)
4023566 .. 4026185
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1042400.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGCGGCTTGGCAGAGGCATCTGCAATCAGCAATCCGTCAAATTGGCAAAAGGGTTGAATGTAGCTACAACCCTTCAGCCAATTTCTCTTCTTCTTCTTCCTTTTCTCGCTTGGAGGGTCTATTGCCTTATTTCCAAACTCTTTGCAAGCCATCTTCTCCAACCATTTCAAGACCCCTTCATCAATATTTTCAGCACTTGGGAATTTCGAGTTCGAGAAACTTGAGAGCTGAGGAAGCGCCTCTTCCATCTCCATTGACTCCTGTTTTGGCATCAAATACTGCGACAAACGAAGAGCACAAGCAGAAATCGCTCACTAAACCTGATAAAGTTCAAGCGATCTTAAAGGGCATCAAACAGAGTCCTAAAAAGGTCAACTTGGTTGCTGCACTGGTACGCGGTATGCGTGTTGAAGATGCATTGTTACAGTTGCAAGTGACAGTAAAGCGAGCTTCAAAAACTGTGTATCAGGTCATTCATTCAGCCCGAGCAAATGCAACTCATAATCATGGGTTAGATCCAGACCGTCTCCTTGTTGCTGAGGCTTTTGTTGGAAAGGGATTCTTCAAAAAGAGACTGTCCTACCATGCCAAAGGCAAATGTGGAGTTAAAGTGAGACCAGAATGCCGCCTTACGGTCGTAGTAAGGGAGATTACCCCTGACGAGGAGGCGGAGATAGCCAAACTGAAGGTTAAGAATTTCATTAAGATGACTAAGCGCGAAAGAAAGCTTGTTCCTCACCAGCTTATTGAGACAACTCCAATTTGGGGCCGCAAAGGCAGAAGTCGCGATCTTGAACAAAATGGTGTGGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

273

Amino Acids

30.46

Weight (kDa)

10.23

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 113 - 216 7.4e-29 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 399, 720, 789
AciI CCGC 5 cut(s) 5, 399, 628, 671, 772
AclWI GGATC 1 cut(s) 512
AcsI RAATTY 2 cut(s) 204, 697
AcuI CTGAAG 2 cut(s) 60, 707
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 3 cut(s) 634, 664, 767
AgsI TTSAA 7 cut(s) 59, 171, 335, 413, 456, 568, 797
AluBI AGCT 5 cut(s) 66, 230, 452, 730, 745
AluI AGCT 5 cut(s) 66, 230, 452, 730, 745
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 4 cut(s) 533, 568, 610, 746
AlwI GGATC 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 1 cut(s) 769
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 2 cut(s) 204, 697
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AspLEI GCGC 2 cut(s) 241, 720
AspS9I GGNCC 1 cut(s) 769
AsuHPI GGTGA 1 cut(s) 731
AvaI CYCGRG 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 2 cut(s) 231, 540
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 160, 256
BcoDI GTCTC 4 cut(s) 533, 568, 610, 746
BfoI RGCGCY 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 777
BisI GCNGC 4 cut(s) 6, 387, 628, 772
BlsI GCNGC 4 cut(s) 7, 388, 629, 773
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 769
BmiI GGNNCC 1 cut(s) 770
BmsI GCATC 4 cut(s) 28, 281, 360, 406
Bpu10I CCTNAGC 2 cut(s) 231, 540
BpuEI CTTGAG 1 cut(s) 244
BsaI GGTCTC 1 cut(s) 610
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 3 cut(s) 634, 664, 767
Bse1I ACTGG 1 cut(s) 396
BseLI CCNNNNNNNGG 3 cut(s) 634, 664, 767
BseMII CTCAG 2 cut(s) 222, 531
BseNI ACTGG 1 cut(s) 396
BseRI GAGGAG 1 cut(s) 680
BseXI GCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 372
Bsh1236I CGCG 3 cut(s) 399, 720, 789
Bsh1285I CGRYCG 1 cut(s) 639
BshFI GGCC 1 cut(s) 771
BsiEI CGRYCG 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 486
BslI CCNNNNNNNGG 3 cut(s) 634, 664, 767
BsmAI GTCTC 4 cut(s) 533, 568, 610, 746
BsmBI CGTCTC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 1 cut(s) 771
Bso31I GGTCTC 1 cut(s) 610
BsoBI CYCGRG 1 cut(s) 486
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 3 cut(s) 339, 517, 790
Bsp68I TCGCGA 1 cut(s) 789
BspACI CCGC 5 cut(s) 5, 399, 628, 671, 772
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 2 cut(s) 223, 532
BspFNI CGCG 3 cut(s) 399, 720, 789
BspHI TCATGA 1 cut(s) 815
BspLI GGNNCC 1 cut(s) 770
BspPI GGATC 1 cut(s) 512
BspQI GCTCTTC 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 610
BsrI ACTGG 1 cut(s) 396
BssMI GATC 3 cut(s) 339, 517, 790
Bst4CI ACNGT 6 cut(s) 429, 442, 463, 527, 579, 637
Bst6I CTCTTC 3 cut(s) 94, 249, 288
BstC8I GCNNGC 2 cut(s) 149, 450
BstDEI CTNAG 3 cut(s) 231, 540, 714
BstFNI CGCG 3 cut(s) 399, 720, 789
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 2 cut(s) 241, 720
BstKTI GATC 3 cut(s) 342, 520, 793
BstMAI GTCTC 4 cut(s) 533, 568, 610, 746
BstMBI GATC 3 cut(s) 339, 517, 790
BstMCI CGRYCG 1 cut(s) 639
BstMWI GCNNNNNNNGC 5 cut(s) 236, 310, 677, 777, 786
BstUI CGCG 3 cut(s) 399, 720, 789
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BsuRI GGCC 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 389
BtuMI TCGCGA 1 cut(s) 789
Cac8I GCNNGC 2 cut(s) 149, 450
CciI TCATGA 1 cut(s) 815
CfoI GCGC 2 cut(s) 241, 720
Cfr13I GGNCC 1 cut(s) 769
Csp6I GTAC 1 cut(s) 395
CviAII CATG 3 cut(s) 509, 587, 816
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 3 cut(s) 231, 540, 714
DpnI GATC 3 cut(s) 341, 519, 792
DpnII GATC 3 cut(s) 339, 517, 790
Eam1104I CTCTTC 3 cut(s) 94, 249, 288
EarI CTCTTC 3 cut(s) 94, 249, 288
EciI GGCGGA 1 cut(s) 686
Eco31I GGTCTC 1 cut(s) 610
Eco57I CTGAAG 2 cut(s) 60, 707
Eco88I CYCGRG 1 cut(s) 486
EcoT22I ATGCAT 1 cut(s) 421
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 3 cut(s) 512, 590, 819
FaiI YATR 5 cut(s) 404, 504, 510, 588, 817
FalI AAGNNNNNCTT 6 cut(s) 228, 260, 362, 394, 548, 580
FatI CATG 3 cut(s) 508, 586, 815
Fnu4HI GCNGC 4 cut(s) 6, 387, 628, 772
Fsp4HI GCNGC 4 cut(s) 6, 387, 628, 772
GlaI GCGC 2 cut(s) 240, 719
GluI GCNGC 4 cut(s) 6, 387, 628, 772
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 1 cut(s) 771
HhaI GCGC 2 cut(s) 241, 720
Hin1II CATG 3 cut(s) 512, 590, 819
Hin6I GCGC 2 cut(s) 239, 718
HinP1I GCGC 2 cut(s) 239, 718
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 3 cut(s) 259, 361, 561
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 1 cut(s) 376
Hpy188III TCNNGA 6 cut(s) 171, 216, 521, 788, 794, 816
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 3 cut(s) 84, 188, 682
HpyCH4III ACNGT 6 cut(s) 429, 442, 463, 527, 579, 637
HpyCH4V TGCA 6 cut(s) 25, 147, 389, 419, 433, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 310, 677, 777, 786
HpyF3I CTNAG 3 cut(s) 231, 540, 714
Hsp92II CATG 3 cut(s) 512, 590, 819
HspAI GCGC 2 cut(s) 239, 718
Kzo9I GATC 3 cut(s) 339, 517, 790
LguI GCTCTTC 1 cut(s) 288
LpnPI CCDG 8 cut(s) 276, 336, 377, 455, 534, 633, 672, 755
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 4 cut(s) 28, 281, 360, 406
MaeIII GTNAC 2 cut(s) 423, 436
MalI GATC 3 cut(s) 341, 519, 792
MboI GATC 3 cut(s) 339, 517, 790
MboII GAAGA 9 cut(s) 81, 84, 87, 90, 147, 236, 305, 425, 556
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 5 cut(s) 42, 82, 204, 697, 762
MlyI GAGTC 2 cut(s) 253, 370
MmeI TCCRAC 2 cut(s) 185, 532
MnlI CCTC 8 cut(s) 9, 109, 226, 252, 535, 658, 661, 747
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 4 cut(s) 344, 609, 693, 705
MslI CAYNNNNRTG 2 cut(s) 507, 707
Mva1269I GAATGC 1 cut(s) 629
MvnI CGCG 3 cut(s) 399, 720, 789
MwoI GCNNNNNNNGC 5 cut(s) 236, 310, 677, 777, 786
NdeII GATC 3 cut(s) 339, 517, 790
NlaIII CATG 3 cut(s) 512, 590, 819
NlaIV GGNNCC 1 cut(s) 770
NmuCI GTSAC 1 cut(s) 436
NruI TCGCGA 1 cut(s) 789
NsiI ATGCAT 1 cut(s) 421
PagI TCATGA 1 cut(s) 815
PciSI GCTCTTC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 403
PctI GAATGC 1 cut(s) 629
PfeI GAWTC 1 cut(s) 561
PkrI GCNGC 4 cut(s) 7, 388, 629, 773
PleI GAGTC 2 cut(s) 253, 369
PpsI GAGTC 2 cut(s) 253, 369
PspN4I GGNNCC 1 cut(s) 770
PspPI GGNCC 1 cut(s) 769
PsuI RGATCY 1 cut(s) 517
RruI TCGCGA 1 cut(s) 789
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
RseI CAYNNNNRTG 2 cut(s) 507, 707
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 4 cut(s) 344, 609, 693, 705
SatI GCNGC 4 cut(s) 6, 387, 628, 772
Sau3AI GATC 3 cut(s) 339, 517, 790
Sau96I GGNCC 1 cut(s) 769
SchI GAGTC 2 cut(s) 253, 370
SduI GDGCHC 1 cut(s) 300
SetI ASST 9 cut(s) 68, 232, 325, 375, 454, 474, 693, 732, 747
SfaNI GCATC 4 cut(s) 28, 281, 360, 406
SmiMI CAYNNNNRTG 2 cut(s) 507, 707
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 5 cut(s) 42, 82, 204, 697, 762
SsiI CCGC 5 cut(s) 5, 399, 628, 671, 772
SspI AATATT 1 cut(s) 188
TaaI ACNGT 6 cut(s) 429, 442, 463, 527, 579, 637
TaqI TCGA 2 cut(s) 209, 215
TasI AATT 5 cut(s) 42, 82, 204, 697, 762
TauI GCSGC 3 cut(s) 8, 630, 774
TfiI GAWTC 1 cut(s) 561
Tru1I TTAA 4 cut(s) 344, 609, 693, 705
Tru9I TTAA 4 cut(s) 344, 609, 693, 705
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 4 cut(s) 170, 467, 691, 804
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 396
XapI RAATTY 2 cut(s) 204, 697
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.