MD12G1043300.v1.1

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002533
Physical Location & Seq
Reverse (-)
4866021 .. 4866916
896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1043300.v1.1.491

Sequence Viewer

Length: 363 bp
ATGCATCTTCTTATCGTCATTCATTCAGCCCGAGCAAATGCAACTCATAATCATGGGTTAGACCCAGACCGTCTCCTTGTTGCCGAGGCTTTTGTTGGAAAGGGATTCTTCAAAAAGAGACTGTCCTACCATGCCAAAGGCAAATGTGGAGTGAAAGTGAGACCAGAATGCCGCCTTACGGTCGTAGTGAGGGAGATTACCCCTGACGAGGAGGCAGAGATAGCCAAACTGAAGGTTAAGAATTTCATTAAGATGACTAAGCGCGAAAGAAAGCTTGTTCCTCACCAACTTATTGAGACAACTCCAATTTGGGGCCGCAAAGGCAGAAGTCGTGATAGTGAACGAAATGGTGAGGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

121

Amino Acids

13.66

Weight (kDa)

10.12

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 4 - 64 2.4e-13 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 264
AciI CCGC 2 cut(s) 172, 316
AcsI RAATTY 1 cut(s) 241
AcuI CTGAAG 1 cut(s) 251
AfiI CCNNNNNNNGG 3 cut(s) 178, 208, 311
AgsI TTSAA 1 cut(s) 112
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw26I GTCTC 4 cut(s) 77, 112, 154, 290
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 1 cut(s) 313
ApoI RAATTY 1 cut(s) 241
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 313
AsuHPI GGTGA 2 cut(s) 275, 362
AvaI CYCGRG 1 cut(s) 30
BcoDI GTCTC 4 cut(s) 77, 112, 154, 290
BglI GCCNNNNNGGC 1 cut(s) 321
BisI GCNGC 2 cut(s) 172, 316
BlsI GCNGC 2 cut(s) 173, 317
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 314
BmsI GCATC 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 84
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 208, 311
BseDI CCNNGG 1 cut(s) 84
BseLI CCNNNNNNNGG 3 cut(s) 178, 208, 311
BseRI GAGGAG 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 315
BsiEI CGRYCG 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 30
BslI CCNNNNNNNGG 3 cut(s) 178, 208, 311
BsmAI GTCTC 4 cut(s) 77, 112, 154, 290
BsmBI CGTCTC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 173
BsnI GGCC 1 cut(s) 315
Bso31I GGTCTC 1 cut(s) 154
BsoBI CYCGRG 1 cut(s) 30
BspACI CCGC 2 cut(s) 172, 316
BspANI GGCC 1 cut(s) 315
BspFNI CGCG 1 cut(s) 264
BspHI TCATGA 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 84
Bst4CI ACNGT 3 cut(s) 71, 123, 181
BstDEI CTNAG 1 cut(s) 258
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 1 cut(s) 264
BstMAI GTCTC 4 cut(s) 77, 112, 154, 290
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 2 cut(s) 221, 321
BstUI CGCG 1 cut(s) 264
BsuRI GGCC 1 cut(s) 315
CciI TCATGA 1 cut(s) 359
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 313
CviAII CATG 3 cut(s) 53, 131, 360
CviJI RGCY 6 cut(s) 29, 89, 224, 274, 315, 356
CviKI_1 RGCY 6 cut(s) 29, 89, 224, 274, 315, 356
DdeI CTNAG 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 3 cut(s) 56, 134, 363
FaiI YATR 4 cut(s) 48, 54, 132, 361
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 3 cut(s) 52, 130, 359
Fnu4HI GCNGC 2 cut(s) 172, 316
Fsp4HI GCNGC 2 cut(s) 172, 316
GlaI GCGC 1 cut(s) 263
GluI GCNGC 2 cut(s) 172, 316
HaeIII GGCC 1 cut(s) 315
HhaI GCGC 1 cut(s) 264
Hin1II CATG 3 cut(s) 56, 134, 363
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 1 cut(s) 105
HphI GGTGA 2 cut(s) 275, 362
Hpy166II GTNNAC 1 cut(s) 341
Hpy188III TCNNGA 2 cut(s) 332, 360
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 3 cut(s) 71, 123, 181
HpyCH4V TGCA 2 cut(s) 4, 41
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 321
HpyF3I CTNAG 1 cut(s) 258
Hsp92II CATG 3 cut(s) 56, 134, 363
HspAI GCGC 1 cut(s) 262
LpnPI CCDG 3 cut(s) 78, 177, 216
LweI GCATC 1 cut(s) 13
MboII GAAGA 1 cut(s) 100
MluCI AATT 2 cut(s) 241, 306
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 6 cut(s) 79, 183, 202, 205, 291, 346
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 237, 249
MslI CAYNNNNRTG 2 cut(s) 51, 251
Mva1269I GAATGC 1 cut(s) 173
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 221, 321
NlaIII CATG 3 cut(s) 56, 134, 363
NlaIV GGNNCC 1 cut(s) 314
NmeAIII GCCGAG 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 359
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 105
PkrI GCNGC 2 cut(s) 173, 317
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 313
RseI CAYNNNNRTG 2 cut(s) 51, 251
SaqAI TTAA 2 cut(s) 237, 249
SatI GCNGC 2 cut(s) 172, 316
Sau96I GGNCC 1 cut(s) 313
SetI ASST 2 cut(s) 237, 276
SfaNI GCATC 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 51, 251
Sse9I AATT 2 cut(s) 241, 306
SsiI CCGC 2 cut(s) 172, 316
TaaI ACNGT 3 cut(s) 71, 123, 181
TasI AATT 2 cut(s) 241, 306
TauI GCSGC 2 cut(s) 174, 318
TfiI GAWTC 1 cut(s) 105
Tru1I TTAA 2 cut(s) 237, 249
Tru9I TTAA 2 cut(s) 237, 249
TspDTI ATGAA 3 cut(s) 11, 235, 348
XapI RAATTY 1 cut(s) 241
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.